Inorganic pyrophosphatase from the red flour beetle (Tribolium castaneum) modulates mitochondrial polyphosphate metabolism
Polyphosphates (polyPs) have been found in all cell types examined to date and play diverse roles, depending on the cell type. In eukaryotic organisms, polyPs have been mainly investigated in mammalian cells, with few studies on insects. In this study, we investigated mitochondrial polyphosphate met...
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| Veröffentlicht in: | Archives of insect biochemistry and physiology 2019-12, Vol.102 (4), p.e21606-n/a |
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| creator | Mirra, Bianca Carvalho, Klébea Curitiba, Bianca Ribeiro, Lupis Moraes, Jorge da Silva, José R. Costa, Evenilton P. da Fonseca, Rodrigo N. Campos, Eldo |
| description | Polyphosphates (polyPs) have been found in all cell types examined to date and play diverse roles, depending on the cell type. In eukaryotic organisms, polyPs have been mainly investigated in mammalian cells, with few studies on insects. In this study, we investigated mitochondrial polyphosphate metabolism in the red flour beetle, Tribolium castaneum. Substrate specificity for different chain lengths demonstrated the presence of two exopolyphosphatase isoforms in mitochondria. T. castaneum mitochondrial polyP levels decreased after injection with soluble pyrophosphatase (Tc‐sPPase) dsRNA, while the membrane exopolyphosphate activity increased. Mitochondrial respiration modulated exopolyphosphatase activity only in wild‐type beetles. Tripolyphosphate was able to increase the F‐ATPase activity in wild‐type and Tc‐sPPase RNAi beetles. We suggest that inorganic pyrophosphatase modulates polyphosphate metabolism in mitochondria and affects the link between mitochondrial activity and polyphosphate metabolism in T. castaneum.
The inorganic pyrophosphatase affects polyphosphate and mitochondrial metabolism |
| doi_str_mv | 10.1002/arch.21606 |
| format | Article |
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The inorganic pyrophosphatase affects polyphosphate and mitochondrial metabolism</description><identifier>ISSN: 0739-4462</identifier><identifier>EISSN: 1520-6327</identifier><identifier>DOI: 10.1002/arch.21606</identifier><identifier>PMID: 31498484</identifier><language>eng</language><publisher>United States: Wiley Subscription Services, Inc</publisher><subject>Adenosine triphosphatase ; Adenosine Triphosphatases ; Animals ; Beetles ; Coleoptera ; Double-stranded RNA ; Exopolyphosphatase ; Female ; Flour ; Inorganic pyrophosphatase ; Inorganic Pyrophosphatase - chemistry ; Inorganic Pyrophosphatase - metabolism ; insect ; Insect Proteins - metabolism ; Insects ; Isoforms ; Male ; Mammalian cells ; Metabolism ; Mitochondria ; Mitochondria - metabolism ; polyphosphate ; Polyphosphates ; Polyphosphates - metabolism ; Pyrophosphatase ; RNA Interference ; RNA-mediated interference ; Substrate specificity ; Substrates ; Tribolium - enzymology ; Tribolium - metabolism ; Tribolium castaneum ; Tripolyphosphate</subject><ispartof>Archives of insect biochemistry and physiology, 2019-12, Vol.102 (4), p.e21606-n/a</ispartof><rights>2019 Wiley Periodicals, Inc.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c3576-8ecddcdef72c7ff31a1af141d058f00e28455bcf57cf3ae58f527f61e1719e2e3</citedby><cites>FETCH-LOGICAL-c3576-8ecddcdef72c7ff31a1af141d058f00e28455bcf57cf3ae58f527f61e1719e2e3</cites><orcidid>0000-0003-3668-0397</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1002%2Farch.21606$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1002%2Farch.21606$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>314,776,780,1411,27901,27902,45550,45551</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/31498484$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Mirra, Bianca</creatorcontrib><creatorcontrib>Carvalho, Klébea</creatorcontrib><creatorcontrib>Curitiba, Bianca</creatorcontrib><creatorcontrib>Ribeiro, Lupis</creatorcontrib><creatorcontrib>Moraes, Jorge</creatorcontrib><creatorcontrib>da Silva, José R.</creatorcontrib><creatorcontrib>Costa, Evenilton P.</creatorcontrib><creatorcontrib>da Fonseca, Rodrigo N.</creatorcontrib><creatorcontrib>Campos, Eldo</creatorcontrib><title>Inorganic pyrophosphatase from the red flour beetle (Tribolium castaneum) modulates mitochondrial polyphosphate metabolism</title><title>Archives of insect biochemistry and physiology</title><addtitle>Arch Insect Biochem Physiol</addtitle><description>Polyphosphates (polyPs) have been found in all cell types examined to date and play diverse roles, depending on the cell type. In eukaryotic organisms, polyPs have been mainly investigated in mammalian cells, with few studies on insects. In this study, we investigated mitochondrial polyphosphate metabolism in the red flour beetle, Tribolium castaneum. Substrate specificity for different chain lengths demonstrated the presence of two exopolyphosphatase isoforms in mitochondria. T. castaneum mitochondrial polyP levels decreased after injection with soluble pyrophosphatase (Tc‐sPPase) dsRNA, while the membrane exopolyphosphate activity increased. Mitochondrial respiration modulated exopolyphosphatase activity only in wild‐type beetles. Tripolyphosphate was able to increase the F‐ATPase activity in wild‐type and Tc‐sPPase RNAi beetles. We suggest that inorganic pyrophosphatase modulates polyphosphate metabolism in mitochondria and affects the link between mitochondrial activity and polyphosphate metabolism in T. castaneum.
The inorganic pyrophosphatase affects polyphosphate and mitochondrial metabolism</description><subject>Adenosine triphosphatase</subject><subject>Adenosine Triphosphatases</subject><subject>Animals</subject><subject>Beetles</subject><subject>Coleoptera</subject><subject>Double-stranded RNA</subject><subject>Exopolyphosphatase</subject><subject>Female</subject><subject>Flour</subject><subject>Inorganic pyrophosphatase</subject><subject>Inorganic Pyrophosphatase - chemistry</subject><subject>Inorganic Pyrophosphatase - metabolism</subject><subject>insect</subject><subject>Insect Proteins - metabolism</subject><subject>Insects</subject><subject>Isoforms</subject><subject>Male</subject><subject>Mammalian cells</subject><subject>Metabolism</subject><subject>Mitochondria</subject><subject>Mitochondria - metabolism</subject><subject>polyphosphate</subject><subject>Polyphosphates</subject><subject>Polyphosphates - metabolism</subject><subject>Pyrophosphatase</subject><subject>RNA Interference</subject><subject>RNA-mediated interference</subject><subject>Substrate specificity</subject><subject>Substrates</subject><subject>Tribolium - enzymology</subject><subject>Tribolium - metabolism</subject><subject>Tribolium castaneum</subject><subject>Tripolyphosphate</subject><issn>0739-4462</issn><issn>1520-6327</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2019</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9kcFq3DAQhkVpabZpL32AIuglLTjVjC3LPoalbQKBQkjPQiuPagfJciWbsn36erNJDznkNDB88_EPP2PvQZyDEPjFJNufI9SifsE2IFEUdYnqJdsIVbZFVdV4wt7kfCeEaGtoXrOTEqq2qZpqw_5ejTH9MuNg-bRPcepjnnozm0zcpRj43BNP1HHn45L4jmj2xM9u07CLflgCtybPZqQlfOIhdos3M2UehjnaPo5dGoznU_T7Ry_xQLM53Obwlr1yxmd69zBP2c9vX2-3l8X1j-9X24vrwpZS1UVDtutsR06hVc6VYMA4qKATsnFCEDaVlDvrpLKuNLQuJSpXA4GClpDKU3Z29E4p_l4ozzoM2ZL3a-64ZI3YKAkSFK7oxyfo3fr2uKbTWAIiqhYP1OcjZVPMOZHTUxqCSXsNQh8a0YdG9H0jK_zhQbnsAnX_0ccKVgCOwJ_B0_4Zlb642V4epf8ArciZGQ</recordid><startdate>201912</startdate><enddate>201912</enddate><creator>Mirra, Bianca</creator><creator>Carvalho, Klébea</creator><creator>Curitiba, Bianca</creator><creator>Ribeiro, Lupis</creator><creator>Moraes, Jorge</creator><creator>da Silva, José R.</creator><creator>Costa, Evenilton P.</creator><creator>da Fonseca, Rodrigo N.</creator><creator>Campos, Eldo</creator><general>Wiley Subscription Services, Inc</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QR</scope><scope>7SS</scope><scope>7U9</scope><scope>8FD</scope><scope>FR3</scope><scope>H94</scope><scope>P64</scope><scope>RC3</scope><scope>7X8</scope><orcidid>https://orcid.org/0000-0003-3668-0397</orcidid></search><sort><creationdate>201912</creationdate><title>Inorganic pyrophosphatase from the red flour beetle (Tribolium castaneum) modulates mitochondrial polyphosphate metabolism</title><author>Mirra, Bianca ; Carvalho, Klébea ; Curitiba, Bianca ; Ribeiro, Lupis ; Moraes, Jorge ; da Silva, José R. ; Costa, Evenilton P. ; da Fonseca, Rodrigo N. ; Campos, Eldo</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c3576-8ecddcdef72c7ff31a1af141d058f00e28455bcf57cf3ae58f527f61e1719e2e3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2019</creationdate><topic>Adenosine triphosphatase</topic><topic>Adenosine Triphosphatases</topic><topic>Animals</topic><topic>Beetles</topic><topic>Coleoptera</topic><topic>Double-stranded RNA</topic><topic>Exopolyphosphatase</topic><topic>Female</topic><topic>Flour</topic><topic>Inorganic pyrophosphatase</topic><topic>Inorganic Pyrophosphatase - chemistry</topic><topic>Inorganic Pyrophosphatase - metabolism</topic><topic>insect</topic><topic>Insect Proteins - metabolism</topic><topic>Insects</topic><topic>Isoforms</topic><topic>Male</topic><topic>Mammalian cells</topic><topic>Metabolism</topic><topic>Mitochondria</topic><topic>Mitochondria - metabolism</topic><topic>polyphosphate</topic><topic>Polyphosphates</topic><topic>Polyphosphates - metabolism</topic><topic>Pyrophosphatase</topic><topic>RNA Interference</topic><topic>RNA-mediated interference</topic><topic>Substrate specificity</topic><topic>Substrates</topic><topic>Tribolium - enzymology</topic><topic>Tribolium - metabolism</topic><topic>Tribolium castaneum</topic><topic>Tripolyphosphate</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Mirra, Bianca</creatorcontrib><creatorcontrib>Carvalho, Klébea</creatorcontrib><creatorcontrib>Curitiba, Bianca</creatorcontrib><creatorcontrib>Ribeiro, Lupis</creatorcontrib><creatorcontrib>Moraes, Jorge</creatorcontrib><creatorcontrib>da Silva, José R.</creatorcontrib><creatorcontrib>Costa, Evenilton P.</creatorcontrib><creatorcontrib>da Fonseca, Rodrigo N.</creatorcontrib><creatorcontrib>Campos, Eldo</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Chemoreception Abstracts</collection><collection>Entomology Abstracts (Full archive)</collection><collection>Virology and AIDS Abstracts</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Genetics Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Archives of insect biochemistry and physiology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Mirra, Bianca</au><au>Carvalho, Klébea</au><au>Curitiba, Bianca</au><au>Ribeiro, Lupis</au><au>Moraes, Jorge</au><au>da Silva, José R.</au><au>Costa, Evenilton P.</au><au>da Fonseca, Rodrigo N.</au><au>Campos, Eldo</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Inorganic pyrophosphatase from the red flour beetle (Tribolium castaneum) modulates mitochondrial polyphosphate metabolism</atitle><jtitle>Archives of insect biochemistry and physiology</jtitle><addtitle>Arch Insect Biochem Physiol</addtitle><date>2019-12</date><risdate>2019</risdate><volume>102</volume><issue>4</issue><spage>e21606</spage><epage>n/a</epage><pages>e21606-n/a</pages><issn>0739-4462</issn><eissn>1520-6327</eissn><abstract>Polyphosphates (polyPs) have been found in all cell types examined to date and play diverse roles, depending on the cell type. In eukaryotic organisms, polyPs have been mainly investigated in mammalian cells, with few studies on insects. In this study, we investigated mitochondrial polyphosphate metabolism in the red flour beetle, Tribolium castaneum. Substrate specificity for different chain lengths demonstrated the presence of two exopolyphosphatase isoforms in mitochondria. T. castaneum mitochondrial polyP levels decreased after injection with soluble pyrophosphatase (Tc‐sPPase) dsRNA, while the membrane exopolyphosphate activity increased. Mitochondrial respiration modulated exopolyphosphatase activity only in wild‐type beetles. Tripolyphosphate was able to increase the F‐ATPase activity in wild‐type and Tc‐sPPase RNAi beetles. We suggest that inorganic pyrophosphatase modulates polyphosphate metabolism in mitochondria and affects the link between mitochondrial activity and polyphosphate metabolism in T. castaneum.
The inorganic pyrophosphatase affects polyphosphate and mitochondrial metabolism</abstract><cop>United States</cop><pub>Wiley Subscription Services, Inc</pub><pmid>31498484</pmid><doi>10.1002/arch.21606</doi><tpages>11</tpages><orcidid>https://orcid.org/0000-0003-3668-0397</orcidid></addata></record> |
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| ispartof | Archives of insect biochemistry and physiology, 2019-12, Vol.102 (4), p.e21606-n/a |
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| subjects | Adenosine triphosphatase Adenosine Triphosphatases Animals Beetles Coleoptera Double-stranded RNA Exopolyphosphatase Female Flour Inorganic pyrophosphatase Inorganic Pyrophosphatase - chemistry Inorganic Pyrophosphatase - metabolism insect Insect Proteins - metabolism Insects Isoforms Male Mammalian cells Metabolism Mitochondria Mitochondria - metabolism polyphosphate Polyphosphates Polyphosphates - metabolism Pyrophosphatase RNA Interference RNA-mediated interference Substrate specificity Substrates Tribolium - enzymology Tribolium - metabolism Tribolium castaneum Tripolyphosphate |
| title | Inorganic pyrophosphatase from the red flour beetle (Tribolium castaneum) modulates mitochondrial polyphosphate metabolism |
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