Mechanism of lipid metabolism regulation by soluble dietary fibre from micronized and non-micronized powders of lotus root nodes as revealed by their adsorption and activity inhibition of pancreatic lipase
Soluble dietary fibre (SDF) of micronized and non-micronized powders of lotus root nodes were investigated based on its adsorption and activity inhibition of pancreatic lipase (PL) by using circular dichroism, fluorescence spectroscopy and modification. Results showed that SDF2 (SDF from micronized...
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| Veröffentlicht in: | Food chemistry 2020-02, Vol.305, p.125435-125435, Article 125435 |
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| creator | Chen, Huanhuan Li, Jie Yao, Ruixue Yan, Shoulei Wang, Qingzhang |
| description | Soluble dietary fibre (SDF) of micronized and non-micronized powders of lotus root nodes were investigated based on its adsorption and activity inhibition of pancreatic lipase (PL) by using circular dichroism, fluorescence spectroscopy and modification. Results showed that SDF2 (SDF from micronized powders of lotus root nodes) had stronger PL adsorption and enzyme activity inhibition than SDF1 (SDF from non-micronized powders of lotus root nodes). Specifically, SDF2 showed more binding sites than SDF1 in PL. There were hydrogen bonds and van der Waals interactions between SDF and PL, with Trp on PL probably serving as the main binding site. Carboxyl groups exhibited a stronger inhibition on PL by carboxymethyl and hydroxypropyl modification. The common mechanisms between SDF1 and SDF2 can be attributed to the combination between Trp and carboxyl groups, while the differences may be generated by the variations in structures or chemical groups induced by micronization.
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•SDF2 had stronger adsorption and enzyme activity inhibition ability for PL.•The difference effect of SDF1 and SDF2 on PL may be generated by micronization.•PL inhibition by SDF was dependent on formation of PL-SDF complex.•PL-SDF complex may be combined by the interaction between Trp and carboxyl groups. |
| doi_str_mv | 10.1016/j.foodchem.2019.125435 |
| format | Article |
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[Display omitted]
•SDF2 had stronger adsorption and enzyme activity inhibition ability for PL.•The difference effect of SDF1 and SDF2 on PL may be generated by micronization.•PL inhibition by SDF was dependent on formation of PL-SDF complex.•PL-SDF complex may be combined by the interaction between Trp and carboxyl groups.</description><identifier>ISSN: 0308-8146</identifier><identifier>EISSN: 1873-7072</identifier><identifier>DOI: 10.1016/j.foodchem.2019.125435</identifier><identifier>PMID: 31494497</identifier><language>eng</language><publisher>England: Elsevier Ltd</publisher><subject>Adsorption ; Binding site ; Carboxymethyl and hydroxypropyl modification ; Dietary Fiber ; Fluorescence quenching ; Hydrolysis ; Lipase - metabolism ; Lipid Metabolism ; Lipid metabolism regulation ; Lotus - chemistry ; Lotus root nodes ; Plant Preparations - chemistry ; Plant Roots - chemistry ; Powders - chemistry ; Solubility ; Soluble dietary fibre</subject><ispartof>Food chemistry, 2020-02, Vol.305, p.125435-125435, Article 125435</ispartof><rights>2019 Elsevier Ltd</rights><rights>Copyright © 2019 Elsevier Ltd. All rights reserved.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c405t-4dff7d5c8e80ff8e03083e2d784fafa9c76799eee3c550cf6f7a6b8cb99f646b3</citedby><cites>FETCH-LOGICAL-c405t-4dff7d5c8e80ff8e03083e2d784fafa9c76799eee3c550cf6f7a6b8cb99f646b3</cites><orcidid>0000-0003-4141-1802</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/j.foodchem.2019.125435$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,780,784,3549,27923,27924,45994</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/31494497$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Chen, Huanhuan</creatorcontrib><creatorcontrib>Li, Jie</creatorcontrib><creatorcontrib>Yao, Ruixue</creatorcontrib><creatorcontrib>Yan, Shoulei</creatorcontrib><creatorcontrib>Wang, Qingzhang</creatorcontrib><title>Mechanism of lipid metabolism regulation by soluble dietary fibre from micronized and non-micronized powders of lotus root nodes as revealed by their adsorption and activity inhibition of pancreatic lipase</title><title>Food chemistry</title><addtitle>Food Chem</addtitle><description>Soluble dietary fibre (SDF) of micronized and non-micronized powders of lotus root nodes were investigated based on its adsorption and activity inhibition of pancreatic lipase (PL) by using circular dichroism, fluorescence spectroscopy and modification. Results showed that SDF2 (SDF from micronized powders of lotus root nodes) had stronger PL adsorption and enzyme activity inhibition than SDF1 (SDF from non-micronized powders of lotus root nodes). Specifically, SDF2 showed more binding sites than SDF1 in PL. There were hydrogen bonds and van der Waals interactions between SDF and PL, with Trp on PL probably serving as the main binding site. Carboxyl groups exhibited a stronger inhibition on PL by carboxymethyl and hydroxypropyl modification. The common mechanisms between SDF1 and SDF2 can be attributed to the combination between Trp and carboxyl groups, while the differences may be generated by the variations in structures or chemical groups induced by micronization.
[Display omitted]
•SDF2 had stronger adsorption and enzyme activity inhibition ability for PL.•The difference effect of SDF1 and SDF2 on PL may be generated by micronization.•PL inhibition by SDF was dependent on formation of PL-SDF complex.•PL-SDF complex may be combined by the interaction between Trp and carboxyl groups.</description><subject>Adsorption</subject><subject>Binding site</subject><subject>Carboxymethyl and hydroxypropyl modification</subject><subject>Dietary Fiber</subject><subject>Fluorescence quenching</subject><subject>Hydrolysis</subject><subject>Lipase - metabolism</subject><subject>Lipid Metabolism</subject><subject>Lipid metabolism regulation</subject><subject>Lotus - chemistry</subject><subject>Lotus root nodes</subject><subject>Plant Preparations - chemistry</subject><subject>Plant Roots - chemistry</subject><subject>Powders - chemistry</subject><subject>Solubility</subject><subject>Soluble dietary fibre</subject><issn>0308-8146</issn><issn>1873-7072</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2020</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkcFu1DAQhi1ERZfCK1Q-csliJ07s3EAVpZWKuJSz5dhj1qvEDrazaHlH3glnt0XcOFkefTP_zP8jdE3JlhLavd9vbQhG72Da1oT2W1q3rGlfoA0VvKk44fVLtCENEZWgrLtEr1PaE0IKK16hy4aynrGeb9DvL6B3yrs04WDx6GZn8ARZDWFcaxG-L6PKLng8HHEK4zKMgI0rRDxi64YI2MYw4cnpGLz7BQYrb7APvvqnNIefBmI6SYS8JBxDyAUykLAqPziAGgtXNPIOXMTKpBDnk-46TunsDi4fsfM7N7hTvcyaldcRynp63VwleIMurBoTvH16r9C320-PN3fVw9fP9zcfHyrNSJsrZqzlptUCBLFWwOpTA7XhglllVa95x_seABrdtkTbznLVDUIPfW871g3NFXp3njvH8GOBlOXkkoZxVB7CkmRdC95SxpquoN0ZLWakFMHKObqpuCcpkWuUci-fo5RrlPIcZWm8ftJYhgnM37bn7Arw4QxAufTgIMqkHXgNxkXQWZrg_qfxB685unU</recordid><startdate>20200201</startdate><enddate>20200201</enddate><creator>Chen, Huanhuan</creator><creator>Li, Jie</creator><creator>Yao, Ruixue</creator><creator>Yan, Shoulei</creator><creator>Wang, Qingzhang</creator><general>Elsevier Ltd</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><orcidid>https://orcid.org/0000-0003-4141-1802</orcidid></search><sort><creationdate>20200201</creationdate><title>Mechanism of lipid metabolism regulation by soluble dietary fibre from micronized and non-micronized powders of lotus root nodes as revealed by their adsorption and activity inhibition of pancreatic lipase</title><author>Chen, Huanhuan ; Li, Jie ; Yao, Ruixue ; Yan, Shoulei ; Wang, Qingzhang</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c405t-4dff7d5c8e80ff8e03083e2d784fafa9c76799eee3c550cf6f7a6b8cb99f646b3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2020</creationdate><topic>Adsorption</topic><topic>Binding site</topic><topic>Carboxymethyl and hydroxypropyl modification</topic><topic>Dietary Fiber</topic><topic>Fluorescence quenching</topic><topic>Hydrolysis</topic><topic>Lipase - metabolism</topic><topic>Lipid Metabolism</topic><topic>Lipid metabolism regulation</topic><topic>Lotus - chemistry</topic><topic>Lotus root nodes</topic><topic>Plant Preparations - chemistry</topic><topic>Plant Roots - chemistry</topic><topic>Powders - chemistry</topic><topic>Solubility</topic><topic>Soluble dietary fibre</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Chen, Huanhuan</creatorcontrib><creatorcontrib>Li, Jie</creatorcontrib><creatorcontrib>Yao, Ruixue</creatorcontrib><creatorcontrib>Yan, Shoulei</creatorcontrib><creatorcontrib>Wang, Qingzhang</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Food chemistry</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Chen, Huanhuan</au><au>Li, Jie</au><au>Yao, Ruixue</au><au>Yan, Shoulei</au><au>Wang, Qingzhang</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Mechanism of lipid metabolism regulation by soluble dietary fibre from micronized and non-micronized powders of lotus root nodes as revealed by their adsorption and activity inhibition of pancreatic lipase</atitle><jtitle>Food chemistry</jtitle><addtitle>Food Chem</addtitle><date>2020-02-01</date><risdate>2020</risdate><volume>305</volume><spage>125435</spage><epage>125435</epage><pages>125435-125435</pages><artnum>125435</artnum><issn>0308-8146</issn><eissn>1873-7072</eissn><abstract>Soluble dietary fibre (SDF) of micronized and non-micronized powders of lotus root nodes were investigated based on its adsorption and activity inhibition of pancreatic lipase (PL) by using circular dichroism, fluorescence spectroscopy and modification. Results showed that SDF2 (SDF from micronized powders of lotus root nodes) had stronger PL adsorption and enzyme activity inhibition than SDF1 (SDF from non-micronized powders of lotus root nodes). Specifically, SDF2 showed more binding sites than SDF1 in PL. There were hydrogen bonds and van der Waals interactions between SDF and PL, with Trp on PL probably serving as the main binding site. Carboxyl groups exhibited a stronger inhibition on PL by carboxymethyl and hydroxypropyl modification. The common mechanisms between SDF1 and SDF2 can be attributed to the combination between Trp and carboxyl groups, while the differences may be generated by the variations in structures or chemical groups induced by micronization.
[Display omitted]
•SDF2 had stronger adsorption and enzyme activity inhibition ability for PL.•The difference effect of SDF1 and SDF2 on PL may be generated by micronization.•PL inhibition by SDF was dependent on formation of PL-SDF complex.•PL-SDF complex may be combined by the interaction between Trp and carboxyl groups.</abstract><cop>England</cop><pub>Elsevier Ltd</pub><pmid>31494497</pmid><doi>10.1016/j.foodchem.2019.125435</doi><tpages>1</tpages><orcidid>https://orcid.org/0000-0003-4141-1802</orcidid></addata></record> |
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| subjects | Adsorption Binding site Carboxymethyl and hydroxypropyl modification Dietary Fiber Fluorescence quenching Hydrolysis Lipase - metabolism Lipid Metabolism Lipid metabolism regulation Lotus - chemistry Lotus root nodes Plant Preparations - chemistry Plant Roots - chemistry Powders - chemistry Solubility Soluble dietary fibre |
| title | Mechanism of lipid metabolism regulation by soluble dietary fibre from micronized and non-micronized powders of lotus root nodes as revealed by their adsorption and activity inhibition of pancreatic lipase |
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