Preparative separation of isoquinoline alkaloids from Corydalis impatiens using a middle‐pressure chromatogram isolated gel column coupled with two‐dimensional liquid chromatography
We established a two‐dimensional strong cation exchange/reversed‐phase liquid chromatography protocol to isolate and purify isoquinoline alkaloids from Corydalis impatiens. Isoquinoline alkaloids were first enriched from a C. impatiens extract in which liposoluble components were removed using a med...
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| Veröffentlicht in: | Journal of separation science 2019-10, Vol.42 (20), p.3182-3190 |
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| container_title | Journal of separation science |
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| creator | Pan, Guoqing Shen, Jianwei Ma, Yuhua He, Yanfeng Bao, Yi Li, Rongrong Wang, Shunshan Wang, Qi Lin, Pengcheng Dang, Jun |
| description | We established a two‐dimensional strong cation exchange/reversed‐phase liquid chromatography protocol to isolate and purify isoquinoline alkaloids from Corydalis impatiens. Isoquinoline alkaloids were first enriched from a C. impatiens extract in which liposoluble components were removed using a medium‐pressure chromatographic tower containing middle chromatogram isolated gel. A strong cation exchange column was employed to separate and obtain 30 fractions. We chose fractions 22–29 for reversed‐phase liquid chromatography purification using characteristic isoquinoline alkaloid ultraviolet absorption spectra. Several isoquinoline alkaloid fractions (22–29) were further separated, and those of low resolution were isolated via two‐dimensional liquid chromatography in the orthogonal plane. A total of eight novel isoquinoline alkaloids with characteristic ultraviolet spectra were obtained from C. impatiens. We thus demonstrate the benefits of off‐line two‐dimensional strong cation exchange/reversed‐phase liquid chromatography to isolate isoquinoline alkaloids from C. impatiens. |
| doi_str_mv | 10.1002/jssc.201900252 |
| format | Article |
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Isoquinoline alkaloids were first enriched from a C. impatiens extract in which liposoluble components were removed using a medium‐pressure chromatographic tower containing middle chromatogram isolated gel. A strong cation exchange column was employed to separate and obtain 30 fractions. We chose fractions 22–29 for reversed‐phase liquid chromatography purification using characteristic isoquinoline alkaloid ultraviolet absorption spectra. Several isoquinoline alkaloid fractions (22–29) were further separated, and those of low resolution were isolated via two‐dimensional liquid chromatography in the orthogonal plane. A total of eight novel isoquinoline alkaloids with characteristic ultraviolet spectra were obtained from C. impatiens. We thus demonstrate the benefits of off‐line two‐dimensional strong cation exchange/reversed‐phase liquid chromatography to isolate isoquinoline alkaloids from C. impatiens.</description><identifier>ISSN: 1615-9306</identifier><identifier>EISSN: 1615-9314</identifier><identifier>DOI: 10.1002/jssc.201900252</identifier><identifier>PMID: 31429171</identifier><language>eng</language><publisher>Germany: Wiley Subscription Services, Inc</publisher><subject>Absorption spectra ; Alkaloids ; Cation exchanging ; Chromatography ; Corydalis impatiens ; Ions ; isoquinoline alkaloids ; Liquid chromatography ; preparative separation ; two‐dimensional liquid chromatography ; Ultraviolet absorption ; Ultraviolet spectra</subject><ispartof>Journal of separation science, 2019-10, Vol.42 (20), p.3182-3190</ispartof><rights>2019 WILEY‐VCH Verlag GmbH & Co. KGaA, Weinheim</rights><rights>2019 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c4055-847c79ae8dd50e9b952af3953fa4b4b747c50a4aa9211843a4eabe82a40fcbb73</citedby><cites>FETCH-LOGICAL-c4055-847c79ae8dd50e9b952af3953fa4b4b747c50a4aa9211843a4eabe82a40fcbb73</cites><orcidid>0000-0003-4206-2587</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1002%2Fjssc.201900252$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1002%2Fjssc.201900252$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>314,780,784,1417,27924,27925,45574,45575</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/31429171$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Pan, Guoqing</creatorcontrib><creatorcontrib>Shen, Jianwei</creatorcontrib><creatorcontrib>Ma, Yuhua</creatorcontrib><creatorcontrib>He, Yanfeng</creatorcontrib><creatorcontrib>Bao, Yi</creatorcontrib><creatorcontrib>Li, Rongrong</creatorcontrib><creatorcontrib>Wang, Shunshan</creatorcontrib><creatorcontrib>Wang, Qi</creatorcontrib><creatorcontrib>Lin, Pengcheng</creatorcontrib><creatorcontrib>Dang, Jun</creatorcontrib><title>Preparative separation of isoquinoline alkaloids from Corydalis impatiens using a middle‐pressure chromatogram isolated gel column coupled with two‐dimensional liquid chromatography</title><title>Journal of separation science</title><addtitle>J Sep Sci</addtitle><description>We established a two‐dimensional strong cation exchange/reversed‐phase liquid chromatography protocol to isolate and purify isoquinoline alkaloids from Corydalis impatiens. Isoquinoline alkaloids were first enriched from a C. impatiens extract in which liposoluble components were removed using a medium‐pressure chromatographic tower containing middle chromatogram isolated gel. A strong cation exchange column was employed to separate and obtain 30 fractions. We chose fractions 22–29 for reversed‐phase liquid chromatography purification using characteristic isoquinoline alkaloid ultraviolet absorption spectra. Several isoquinoline alkaloid fractions (22–29) were further separated, and those of low resolution were isolated via two‐dimensional liquid chromatography in the orthogonal plane. A total of eight novel isoquinoline alkaloids with characteristic ultraviolet spectra were obtained from C. impatiens. We thus demonstrate the benefits of off‐line two‐dimensional strong cation exchange/reversed‐phase liquid chromatography to isolate isoquinoline alkaloids from C. impatiens.</description><subject>Absorption spectra</subject><subject>Alkaloids</subject><subject>Cation exchanging</subject><subject>Chromatography</subject><subject>Corydalis impatiens</subject><subject>Ions</subject><subject>isoquinoline alkaloids</subject><subject>Liquid chromatography</subject><subject>preparative separation</subject><subject>two‐dimensional liquid chromatography</subject><subject>Ultraviolet absorption</subject><subject>Ultraviolet spectra</subject><issn>1615-9306</issn><issn>1615-9314</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2019</creationdate><recordtype>article</recordtype><recordid>eNqFkcFuEzEQhi1ERUvhyhFZ4sIlqe21s-sjikoBVWqlwnk1u55NHOz1Yu8S5cYj9HV4HZ4ERwkR4sJpxp7v_zX2T8grzuacMXG1SamdC8Z1PijxhFzwBVczXXD59NSzxTl5ntKGMV5Wmj0j53ksNC_5Bfl5H3GACKP9jjQd29DT0FGbwrfJ9sHZHim4r-CCNYl2MXi6DHFnwNlErR-yAvtEp2T7FQXqrTEOf_14HCKmNEWk7TprYAyrCH5v62BEQ1foaBvc5PtcpsHlq60d13Tchiw21mfTvAo46mxexPxtM6x3L8hZBy7hy2O9JF_eX39efpjd3t18XL67nbWSKTWrZNmWGrAyRjHUjVYCukKrogPZyKbMY8VAAmjBeSULkAgNVgIk69qmKYtL8vbgO8T8H5jG2tvUonPQY5hSLURVKi4LxTP65h90E6aYX5CpgpUyJ1HtqfmBamNIKWJXD9F6iLuas3ofar0PtT6FmgWvj7ZT49Gc8D8pZkAegK11uPuPXf3p4WG5UEoVvwEfJLWo</recordid><startdate>201910</startdate><enddate>201910</enddate><creator>Pan, Guoqing</creator><creator>Shen, Jianwei</creator><creator>Ma, Yuhua</creator><creator>He, Yanfeng</creator><creator>Bao, Yi</creator><creator>Li, Rongrong</creator><creator>Wang, Shunshan</creator><creator>Wang, Qi</creator><creator>Lin, Pengcheng</creator><creator>Dang, Jun</creator><general>Wiley Subscription Services, Inc</general><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7U5</scope><scope>8FD</scope><scope>L7M</scope><scope>7X8</scope><orcidid>https://orcid.org/0000-0003-4206-2587</orcidid></search><sort><creationdate>201910</creationdate><title>Preparative separation of isoquinoline alkaloids from Corydalis impatiens using a middle‐pressure chromatogram isolated gel column coupled with two‐dimensional liquid chromatography</title><author>Pan, Guoqing ; Shen, Jianwei ; Ma, Yuhua ; He, Yanfeng ; Bao, Yi ; Li, Rongrong ; Wang, Shunshan ; Wang, Qi ; Lin, Pengcheng ; Dang, Jun</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c4055-847c79ae8dd50e9b952af3953fa4b4b747c50a4aa9211843a4eabe82a40fcbb73</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2019</creationdate><topic>Absorption spectra</topic><topic>Alkaloids</topic><topic>Cation exchanging</topic><topic>Chromatography</topic><topic>Corydalis impatiens</topic><topic>Ions</topic><topic>isoquinoline alkaloids</topic><topic>Liquid chromatography</topic><topic>preparative separation</topic><topic>two‐dimensional liquid chromatography</topic><topic>Ultraviolet absorption</topic><topic>Ultraviolet spectra</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Pan, Guoqing</creatorcontrib><creatorcontrib>Shen, Jianwei</creatorcontrib><creatorcontrib>Ma, Yuhua</creatorcontrib><creatorcontrib>He, Yanfeng</creatorcontrib><creatorcontrib>Bao, Yi</creatorcontrib><creatorcontrib>Li, Rongrong</creatorcontrib><creatorcontrib>Wang, Shunshan</creatorcontrib><creatorcontrib>Wang, Qi</creatorcontrib><creatorcontrib>Lin, Pengcheng</creatorcontrib><creatorcontrib>Dang, Jun</creatorcontrib><collection>PubMed</collection><collection>CrossRef</collection><collection>Solid State and Superconductivity Abstracts</collection><collection>Technology Research Database</collection><collection>Advanced Technologies Database with Aerospace</collection><collection>MEDLINE - Academic</collection><jtitle>Journal of separation science</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Pan, Guoqing</au><au>Shen, Jianwei</au><au>Ma, Yuhua</au><au>He, Yanfeng</au><au>Bao, Yi</au><au>Li, Rongrong</au><au>Wang, Shunshan</au><au>Wang, Qi</au><au>Lin, Pengcheng</au><au>Dang, Jun</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Preparative separation of isoquinoline alkaloids from Corydalis impatiens using a middle‐pressure chromatogram isolated gel column coupled with two‐dimensional liquid chromatography</atitle><jtitle>Journal of separation science</jtitle><addtitle>J Sep Sci</addtitle><date>2019-10</date><risdate>2019</risdate><volume>42</volume><issue>20</issue><spage>3182</spage><epage>3190</epage><pages>3182-3190</pages><issn>1615-9306</issn><eissn>1615-9314</eissn><abstract>We established a two‐dimensional strong cation exchange/reversed‐phase liquid chromatography protocol to isolate and purify isoquinoline alkaloids from Corydalis impatiens. Isoquinoline alkaloids were first enriched from a C. impatiens extract in which liposoluble components were removed using a medium‐pressure chromatographic tower containing middle chromatogram isolated gel. A strong cation exchange column was employed to separate and obtain 30 fractions. We chose fractions 22–29 for reversed‐phase liquid chromatography purification using characteristic isoquinoline alkaloid ultraviolet absorption spectra. Several isoquinoline alkaloid fractions (22–29) were further separated, and those of low resolution were isolated via two‐dimensional liquid chromatography in the orthogonal plane. A total of eight novel isoquinoline alkaloids with characteristic ultraviolet spectra were obtained from C. impatiens. We thus demonstrate the benefits of off‐line two‐dimensional strong cation exchange/reversed‐phase liquid chromatography to isolate isoquinoline alkaloids from C. impatiens.</abstract><cop>Germany</cop><pub>Wiley Subscription Services, Inc</pub><pmid>31429171</pmid><doi>10.1002/jssc.201900252</doi><tpages>9</tpages><orcidid>https://orcid.org/0000-0003-4206-2587</orcidid></addata></record> |
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| subjects | Absorption spectra Alkaloids Cation exchanging Chromatography Corydalis impatiens Ions isoquinoline alkaloids Liquid chromatography preparative separation two‐dimensional liquid chromatography Ultraviolet absorption Ultraviolet spectra |
| title | Preparative separation of isoquinoline alkaloids from Corydalis impatiens using a middle‐pressure chromatogram isolated gel column coupled with two‐dimensional liquid chromatography |
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