MiR-202-5p/MATN2 are associated with regulatory T-cells differentiation and function in allergic rhinitis
Regulatory T cells (Tregs) play a crucial role in allergic rhinitis (AR). However, the mechanism of how Tregs are regulated in AR is poorly understood. Here, we aimed to explore the role of Tregs in AR and how Tregs were regulated by miR-202-5P, which was demonstrated to be important in AR. Peripher...
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| Veröffentlicht in: | Human cell : official journal of Human Cell Research Society 2019-10, Vol.32 (4), p.411-417 |
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| description | Regulatory T cells (Tregs) play a crucial role in allergic rhinitis (AR). However, the mechanism of how Tregs are regulated in AR is poorly understood. Here, we aimed to explore the role of Tregs in AR and how Tregs were regulated by miR-202-5P, which was demonstrated to be important in AR. Peripheral blood mononuclear cells (PBMC) were isolated from collected blood samples. Tregs were purified using Regulatory T Cell Isolation Kit, and differentiated from isolated CD4 T cells using recombinant human interleukin-2 (rhIL-2) and transforming growth factor beta (TGF-β). mRNA expression levels of miR-202-5p, matrilin-2 (MATN2), TGF-β1 and interleukin-10 (IL-10) were detected by real-time PCR. The concentrations of IL-4, interleukin-17 (IL-17), IL-10, interferon gamma (IFN-γ) and TGF-β1 were detected by enzyme-linked immunosorbent assay (ELISA). MATN2 protein level was detected by Western blot. MiR-202-5p expression dramatically elevated in PBMCs, CD4+ T cells and Tregs of AR patients. In vitro, miR-202-5p promoted Tregs differentiation via targeting MATN2. MiR-202-5p/MATN2 axis mediated Tregs proliferation and functions. MiR-202-5p/MATN2 are associated with regulatory T-cells differentiation and function in allergic rhinitis. |
| doi_str_mv | 10.1007/s13577-019-00266-0 |
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However, the mechanism of how Tregs are regulated in AR is poorly understood. Here, we aimed to explore the role of Tregs in AR and how Tregs were regulated by miR-202-5P, which was demonstrated to be important in AR. Peripheral blood mononuclear cells (PBMC) were isolated from collected blood samples. Tregs were purified using Regulatory T Cell Isolation Kit, and differentiated from isolated CD4 T cells using recombinant human interleukin-2 (rhIL-2) and transforming growth factor beta (TGF-β). mRNA expression levels of miR-202-5p, matrilin-2 (MATN2), TGF-β1 and interleukin-10 (IL-10) were detected by real-time PCR. The concentrations of IL-4, interleukin-17 (IL-17), IL-10, interferon gamma (IFN-γ) and TGF-β1 were detected by enzyme-linked immunosorbent assay (ELISA). MATN2 protein level was detected by Western blot. MiR-202-5p expression dramatically elevated in PBMCs, CD4+ T cells and Tregs of AR patients. In vitro, miR-202-5p promoted Tregs differentiation via targeting MATN2. MiR-202-5p/MATN2 axis mediated Tregs proliferation and functions. MiR-202-5p/MATN2 are associated with regulatory T-cells differentiation and function in allergic rhinitis.</description><identifier>ISSN: 1749-0774</identifier><identifier>ISSN: 0914-7470</identifier><identifier>EISSN: 1749-0774</identifier><identifier>DOI: 10.1007/s13577-019-00266-0</identifier><identifier>PMID: 31493245</identifier><language>eng</language><publisher>Tokyo: Springer Japan</publisher><subject>Adult ; Allergic rhinitis ; Biomedical and Life Sciences ; CD4 antigen ; Cell Biology ; Cell differentiation ; Cell Differentiation - genetics ; Cell proliferation ; Cytokines ; Enzyme-linked immunosorbent assay ; Female ; Gene Expression ; Gynecology ; Humans ; Immunoregulation ; Interleukin 10 ; Interleukin 17 ; Interleukin 2 ; Interleukin 4 ; Leukocytes (mononuclear) ; Life Sciences ; Lymphocytes ; Lymphocytes T ; Male ; Matrilin Proteins - genetics ; Matrilin Proteins - metabolism ; Matrilin Proteins - physiology ; MicroRNAs - genetics ; MicroRNAs - physiology ; Middle Aged ; Molecular Targeted Therapy ; Nose ; Oncology ; Peripheral blood mononuclear cells ; Reproductive Medicine ; Research Article ; Rhinitis, Allergic - drug therapy ; Rhinitis, Allergic - genetics ; Rhinitis, Allergic - immunology ; RNA, Messenger - genetics ; RNA, Messenger - metabolism ; Stem Cells ; Surgery ; T-Lymphocytes, Regulatory - immunology ; T-Lymphocytes, Regulatory - physiology ; Transforming growth factor-b1 ; Young Adult ; γ-Interferon</subject><ispartof>Human cell : official journal of Human Cell Research Society, 2019-10, Vol.32 (4), p.411-417</ispartof><rights>Japan Human Cell Society and Springer Japan KK, part of Springer Nature 2019</rights><rights>Copyright Springer Nature B.V. 2019</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c399t-8e9d6805882d0b6ecce7c7d65f5c13f32da6d12c9ffe43c7dc922f7f83f11a983</citedby><cites>FETCH-LOGICAL-c399t-8e9d6805882d0b6ecce7c7d65f5c13f32da6d12c9ffe43c7dc922f7f83f11a983</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://link.springer.com/content/pdf/10.1007/s13577-019-00266-0$$EPDF$$P50$$Gspringer$$H</linktopdf><linktohtml>$$Uhttps://link.springer.com/10.1007/s13577-019-00266-0$$EHTML$$P50$$Gspringer$$H</linktohtml><link.rule.ids>315,781,785,27929,27930,41493,42562,51324</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/31493245$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Wang, Li</creatorcontrib><creatorcontrib>Yang, Xin</creatorcontrib><creatorcontrib>Li, Wencai</creatorcontrib><creatorcontrib>Song, Xicheng</creatorcontrib><creatorcontrib>Kang, Shasha</creatorcontrib><title>MiR-202-5p/MATN2 are associated with regulatory T-cells differentiation and function in allergic rhinitis</title><title>Human cell : official journal of Human Cell Research Society</title><addtitle>Human Cell</addtitle><addtitle>Hum Cell</addtitle><description>Regulatory T cells (Tregs) play a crucial role in allergic rhinitis (AR). However, the mechanism of how Tregs are regulated in AR is poorly understood. Here, we aimed to explore the role of Tregs in AR and how Tregs were regulated by miR-202-5P, which was demonstrated to be important in AR. Peripheral blood mononuclear cells (PBMC) were isolated from collected blood samples. Tregs were purified using Regulatory T Cell Isolation Kit, and differentiated from isolated CD4 T cells using recombinant human interleukin-2 (rhIL-2) and transforming growth factor beta (TGF-β). mRNA expression levels of miR-202-5p, matrilin-2 (MATN2), TGF-β1 and interleukin-10 (IL-10) were detected by real-time PCR. The concentrations of IL-4, interleukin-17 (IL-17), IL-10, interferon gamma (IFN-γ) and TGF-β1 were detected by enzyme-linked immunosorbent assay (ELISA). MATN2 protein level was detected by Western blot. MiR-202-5p expression dramatically elevated in PBMCs, CD4+ T cells and Tregs of AR patients. In vitro, miR-202-5p promoted Tregs differentiation via targeting MATN2. MiR-202-5p/MATN2 axis mediated Tregs proliferation and functions. MiR-202-5p/MATN2 are associated with regulatory T-cells differentiation and function in allergic rhinitis.</description><subject>Adult</subject><subject>Allergic rhinitis</subject><subject>Biomedical and Life Sciences</subject><subject>CD4 antigen</subject><subject>Cell Biology</subject><subject>Cell differentiation</subject><subject>Cell Differentiation - genetics</subject><subject>Cell proliferation</subject><subject>Cytokines</subject><subject>Enzyme-linked immunosorbent assay</subject><subject>Female</subject><subject>Gene Expression</subject><subject>Gynecology</subject><subject>Humans</subject><subject>Immunoregulation</subject><subject>Interleukin 10</subject><subject>Interleukin 17</subject><subject>Interleukin 2</subject><subject>Interleukin 4</subject><subject>Leukocytes (mononuclear)</subject><subject>Life Sciences</subject><subject>Lymphocytes</subject><subject>Lymphocytes T</subject><subject>Male</subject><subject>Matrilin Proteins - genetics</subject><subject>Matrilin Proteins - metabolism</subject><subject>Matrilin Proteins - physiology</subject><subject>MicroRNAs - genetics</subject><subject>MicroRNAs - physiology</subject><subject>Middle Aged</subject><subject>Molecular Targeted Therapy</subject><subject>Nose</subject><subject>Oncology</subject><subject>Peripheral blood mononuclear cells</subject><subject>Reproductive Medicine</subject><subject>Research Article</subject><subject>Rhinitis, Allergic - drug therapy</subject><subject>Rhinitis, Allergic - genetics</subject><subject>Rhinitis, Allergic - immunology</subject><subject>RNA, Messenger - genetics</subject><subject>RNA, Messenger - metabolism</subject><subject>Stem Cells</subject><subject>Surgery</subject><subject>T-Lymphocytes, Regulatory - immunology</subject><subject>T-Lymphocytes, Regulatory - physiology</subject><subject>Transforming growth factor-b1</subject><subject>Young Adult</subject><subject>γ-Interferon</subject><issn>1749-0774</issn><issn>0914-7470</issn><issn>1749-0774</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2019</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9kUlLBDEQhYMoLqN_wIMEvHiJZunOchRxAxeQ8RxilpkMPd1j0o3MvzfOuOHBU_Kor14V9QA4JPiUYCzOMmG1EAgThTCmnCO8AXaJqIoUotr89d8BeznPMK7qitNtsMNIpRit6l0Q7-MTopiienF2fz5-oNAkD03OnY2m9w6-xX4Kk58Mjem7tIRjZH3TZOhiCD75ti9Y7FpoWgfD0NqViEU3jU-TaGGaxjb2Me-DrWCa7A8-3xF4vrocX9ygu8fr24vzO2SZUj2SXjkucS0ldfiFe2u9sMLxOtSWsMCoM9wRalUZX7FSsYrSIIJkgRCjJBuBk7XvInWvg8-9nsf8sbNpfTdkTankiuJK0YIe_0Fn3ZDasl2hlBRc1kIViq4pm7qckw96keLcpKUmWH8EoddB6BKEXgWhcWk6-rQeXubefbd8Xb4AbA3kUmonPv3M_sf2HR43kwI</recordid><startdate>20191001</startdate><enddate>20191001</enddate><creator>Wang, Li</creator><creator>Yang, Xin</creator><creator>Li, Wencai</creator><creator>Song, Xicheng</creator><creator>Kang, Shasha</creator><general>Springer Japan</general><general>Springer Nature B.V</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>20191001</creationdate><title>MiR-202-5p/MATN2 are associated with regulatory T-cells differentiation and function in allergic rhinitis</title><author>Wang, Li ; Yang, Xin ; Li, Wencai ; Song, Xicheng ; Kang, Shasha</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c399t-8e9d6805882d0b6ecce7c7d65f5c13f32da6d12c9ffe43c7dc922f7f83f11a983</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2019</creationdate><topic>Adult</topic><topic>Allergic rhinitis</topic><topic>Biomedical and Life Sciences</topic><topic>CD4 antigen</topic><topic>Cell Biology</topic><topic>Cell differentiation</topic><topic>Cell Differentiation - genetics</topic><topic>Cell proliferation</topic><topic>Cytokines</topic><topic>Enzyme-linked immunosorbent assay</topic><topic>Female</topic><topic>Gene Expression</topic><topic>Gynecology</topic><topic>Humans</topic><topic>Immunoregulation</topic><topic>Interleukin 10</topic><topic>Interleukin 17</topic><topic>Interleukin 2</topic><topic>Interleukin 4</topic><topic>Leukocytes (mononuclear)</topic><topic>Life Sciences</topic><topic>Lymphocytes</topic><topic>Lymphocytes T</topic><topic>Male</topic><topic>Matrilin Proteins - genetics</topic><topic>Matrilin Proteins - metabolism</topic><topic>Matrilin Proteins - physiology</topic><topic>MicroRNAs - genetics</topic><topic>MicroRNAs - physiology</topic><topic>Middle Aged</topic><topic>Molecular Targeted Therapy</topic><topic>Nose</topic><topic>Oncology</topic><topic>Peripheral blood mononuclear cells</topic><topic>Reproductive Medicine</topic><topic>Research Article</topic><topic>Rhinitis, Allergic - drug therapy</topic><topic>Rhinitis, Allergic - genetics</topic><topic>Rhinitis, Allergic - immunology</topic><topic>RNA, Messenger - genetics</topic><topic>RNA, Messenger - metabolism</topic><topic>Stem Cells</topic><topic>Surgery</topic><topic>T-Lymphocytes, Regulatory - immunology</topic><topic>T-Lymphocytes, Regulatory - physiology</topic><topic>Transforming growth factor-b1</topic><topic>Young Adult</topic><topic>γ-Interferon</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Wang, Li</creatorcontrib><creatorcontrib>Yang, Xin</creatorcontrib><creatorcontrib>Li, Wencai</creatorcontrib><creatorcontrib>Song, Xicheng</creatorcontrib><creatorcontrib>Kang, Shasha</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Human cell : official journal of Human Cell Research Society</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Wang, Li</au><au>Yang, Xin</au><au>Li, Wencai</au><au>Song, Xicheng</au><au>Kang, Shasha</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>MiR-202-5p/MATN2 are associated with regulatory T-cells differentiation and function in allergic rhinitis</atitle><jtitle>Human cell : official journal of Human Cell Research Society</jtitle><stitle>Human Cell</stitle><addtitle>Hum Cell</addtitle><date>2019-10-01</date><risdate>2019</risdate><volume>32</volume><issue>4</issue><spage>411</spage><epage>417</epage><pages>411-417</pages><issn>1749-0774</issn><issn>0914-7470</issn><eissn>1749-0774</eissn><abstract>Regulatory T cells (Tregs) play a crucial role in allergic rhinitis (AR). However, the mechanism of how Tregs are regulated in AR is poorly understood. Here, we aimed to explore the role of Tregs in AR and how Tregs were regulated by miR-202-5P, which was demonstrated to be important in AR. Peripheral blood mononuclear cells (PBMC) were isolated from collected blood samples. Tregs were purified using Regulatory T Cell Isolation Kit, and differentiated from isolated CD4 T cells using recombinant human interleukin-2 (rhIL-2) and transforming growth factor beta (TGF-β). mRNA expression levels of miR-202-5p, matrilin-2 (MATN2), TGF-β1 and interleukin-10 (IL-10) were detected by real-time PCR. The concentrations of IL-4, interleukin-17 (IL-17), IL-10, interferon gamma (IFN-γ) and TGF-β1 were detected by enzyme-linked immunosorbent assay (ELISA). MATN2 protein level was detected by Western blot. MiR-202-5p expression dramatically elevated in PBMCs, CD4+ T cells and Tregs of AR patients. In vitro, miR-202-5p promoted Tregs differentiation via targeting MATN2. MiR-202-5p/MATN2 axis mediated Tregs proliferation and functions. MiR-202-5p/MATN2 are associated with regulatory T-cells differentiation and function in allergic rhinitis.</abstract><cop>Tokyo</cop><pub>Springer Japan</pub><pmid>31493245</pmid><doi>10.1007/s13577-019-00266-0</doi><tpages>7</tpages></addata></record> |
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| subjects | Adult Allergic rhinitis Biomedical and Life Sciences CD4 antigen Cell Biology Cell differentiation Cell Differentiation - genetics Cell proliferation Cytokines Enzyme-linked immunosorbent assay Female Gene Expression Gynecology Humans Immunoregulation Interleukin 10 Interleukin 17 Interleukin 2 Interleukin 4 Leukocytes (mononuclear) Life Sciences Lymphocytes Lymphocytes T Male Matrilin Proteins - genetics Matrilin Proteins - metabolism Matrilin Proteins - physiology MicroRNAs - genetics MicroRNAs - physiology Middle Aged Molecular Targeted Therapy Nose Oncology Peripheral blood mononuclear cells Reproductive Medicine Research Article Rhinitis, Allergic - drug therapy Rhinitis, Allergic - genetics Rhinitis, Allergic - immunology RNA, Messenger - genetics RNA, Messenger - metabolism Stem Cells Surgery T-Lymphocytes, Regulatory - immunology T-Lymphocytes, Regulatory - physiology Transforming growth factor-b1 Young Adult γ-Interferon |
| title | MiR-202-5p/MATN2 are associated with regulatory T-cells differentiation and function in allergic rhinitis |
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