Increased Biomass of Nursery-Grown Douglas-Fir Seedlings upon Inoculation with Diazotrophic Endophytic Consortia
Douglas-fir (Pseudotsuga menziesii) seedlings are periodically challenged by biotic and abiotic stresses. The ability of endophytes to colonize the interior of plants could confer benefits to host plants that may play an important role in plant adaptation to environmental changes. In this greenhouse...
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| Veröffentlicht in: | Forests 2015-10, Vol.6 (10), p.3582-3593 |
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| creator | Khan, Zareen Kandel, Shyam L Ramos, Daniela N Ettl, Gregory J Kim, Soo-Hyung Doty, Sharon L |
| description | Douglas-fir (Pseudotsuga menziesii) seedlings are periodically challenged by biotic and abiotic stresses. The ability of endophytes to colonize the interior of plants could confer benefits to host plants that may play an important role in plant adaptation to environmental changes. In this greenhouse study, nursery-grown Douglas-fir seedlings were inoculated with diazotrophic endophytes previously isolated from poplar and willow trees and grown for fifteen months in nutrient-poor conditions. Inoculated seedlings had significant increases in biomass (48%), root length (13%) and shoot height (16%) compared to the control seedlings. Characterization of these endophytes for symbiotic traits in addition to nitrogen fixation revealed that they can also solubilize phosphate and produce siderophores. Colonization was observed through fluorescent microscopy in seedlings inoculated with gfp- and mkate-tagged strains. Inoculation with beneficial endophytes could prove to be valuable for increasing the production of planting stocks in forest nurseries. |
| doi_str_mv | 10.3390/f6103582 |
| format | Article |
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Inoculation with beneficial endophytes could prove to be valuable for increasing the production of planting stocks in forest nurseries.</description><identifier>ISSN: 1999-4907</identifier><identifier>EISSN: 1999-4907</identifier><identifier>DOI: 10.3390/f6103582</identifier><language>eng</language><publisher>Basel: MDPI AG</publisher><subject>abiotic stress ; Bacteria ; Biomass ; biotic stress ; Endophytes ; Environmental changes ; fluorescence microscopy ; forest nurseries ; Forests ; Fungi ; greenhouse experimentation ; Host plants ; Nitrogen ; Nitrogen fixation ; phosphates ; plant adaptation ; Plant growth ; planting ; Populus ; Pseudotsuga menziesii ; Reforestation ; Seedlings ; siderophores ; trees ; Willow trees</subject><ispartof>Forests, 2015-10, Vol.6 (10), p.3582-3593</ispartof><rights>Copyright MDPI AG 2015</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c355t-b207d9fb25691cac8cf5495061a523bc930fba6059c3829b33c736d2743536003</citedby><cites>FETCH-LOGICAL-c355t-b207d9fb25691cac8cf5495061a523bc930fba6059c3829b33c736d2743536003</cites><orcidid>0000-0002-9546-315X</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,776,780,27901,27902</link.rule.ids></links><search><creatorcontrib>Khan, Zareen</creatorcontrib><creatorcontrib>Kandel, Shyam L</creatorcontrib><creatorcontrib>Ramos, Daniela N</creatorcontrib><creatorcontrib>Ettl, Gregory J</creatorcontrib><creatorcontrib>Kim, Soo-Hyung</creatorcontrib><creatorcontrib>Doty, Sharon L</creatorcontrib><title>Increased Biomass of Nursery-Grown Douglas-Fir Seedlings upon Inoculation with Diazotrophic Endophytic Consortia</title><title>Forests</title><description>Douglas-fir (Pseudotsuga menziesii) seedlings are periodically challenged by biotic and abiotic stresses. 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| source | MDPI - Multidisciplinary Digital Publishing Institute; EZB Electronic Journals Library |
| subjects | abiotic stress Bacteria Biomass biotic stress Endophytes Environmental changes fluorescence microscopy forest nurseries Forests Fungi greenhouse experimentation Host plants Nitrogen Nitrogen fixation phosphates plant adaptation Plant growth planting Populus Pseudotsuga menziesii Reforestation Seedlings siderophores trees Willow trees |
| title | Increased Biomass of Nursery-Grown Douglas-Fir Seedlings upon Inoculation with Diazotrophic Endophytic Consortia |
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