Quantitative real time polymerase chain reaction (qRT‐PCR) and RNAscope in situ hybridization (RNA‐ISH) as effective tools to diagnose feline herpesvirus‐1‐associated dermatitis

Background Felid herpesvirus type 1 (FHV‐1)‐associated dermatitis is characterized by facial and nasal involvement; clinical and histopathological manifestations may overlap with other dermatitides. Objective To evaluate the realibility of qRT‐PCR‐2−ΔΔCq and RNAscope in situ hybridization (RNA‐ISH)...

Ausführliche Beschreibung

Gespeichert in:

Bibliographische Detailangaben
Veröffentlicht in:	Veterinary dermatology 2019-12, Vol.30 (6), p.491-e147
Hauptverfasser:	Mazzei, Maurizio, Vascellari, Marta, Zanardello, Claudia, Melchiotti, Erica, Vannini, Susanna, Forzan, Mario, Marchetti, Veronica, Albanese, Francesco, Abramo, Francesca
Format:	Artikel
Sprache:	eng
Schlagworte:	Animal tissues Animals Cat Diseases - diagnosis Cat Diseases - virology Cats Dermatitis Dermatitis - diagnosis Dermatitis - veterinary Dermatitis - virology DNA, Viral - genetics Face - pathology Face - virology Female Formaldehyde Genes Genomes Herpes viruses Herpesviridae Infections - diagnosis Herpesviridae Infections - veterinary Hybridization In Situ Hybridization - veterinary Inclusion bodies Kinases Male mRNA Paraffin Paraffin Embedding Pets Polymerase chain reaction Real-Time Polymerase Chain Reaction - veterinary Retrospective Studies RNA, Messenger Sensitivity and Specificity Thymidine Varicellovirus - isolation & purification
Online-Zugang:	Volltext
Tags:	Tag hinzufügen Keine Tags, Fügen Sie den ersten Tag hinzu!

Schreiben Sie den ersten Kommentar!