Expression of RAGE in asthmatic rats and the intervention of Roxithromycin

Expression of RAGE in asthmatic rats and the intervention of Roxithromycin

To observe the expression of the Receptor of Advanced glycation end products (RAGE) in asthmatic rats, and explore the intervention of Roxithromycin. A total of 18 Specific Pathogen Free-class Brown Norway male rats were randomly divided into control group, asthma model group and Roxithromycin group...

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Veröffentlicht in:Zhong hua yi xue za zhi 2019-08, Vol.99 (32), p.2542-2546
Hauptverfasser: Gu, X F, Chen, H J, Chen, X M, Xu, T T, Qiu, Z W, Wu, L Q, Dai, W, Ying, S M, Dai, Y R
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container_issue 32
container_start_page 2542
container_title Zhong hua yi xue za zhi
container_volume 99
creator Gu, X F
Chen, H J
Chen, X M
Xu, T T
Qiu, Z W
Wu, L Q
Dai, W
Ying, S M
Dai, Y R
description To observe the expression of the Receptor of Advanced glycation end products (RAGE) in asthmatic rats, and explore the intervention of Roxithromycin. A total of 18 Specific Pathogen Free-class Brown Norway male rats were randomly divided into control group, asthma model group and Roxithromycin group, with 6 rats in each group. The asthmatic model was sensitized by intraperitoneal injection of Ovalbumin (OVA)+Al(OH)(3), and challenged with OVA. Rats in Roxithromycin group were given Roxithromycin 30 mg/kg 30 minutes before each challenge. Rats in control group and asthma model group were treated with equal volume of saline. The concentrations of RAGE and interleukin (IL)-4 in serum and bronchoalveolar lavage fluid (BALF) were measured by enzyme-linked immunosorbent (ELISA); the pathological changes of lung tissues were observed by HE-staining; the thickness of airway wall and airway smooth muscle were measured by Image-Pro Plus; the relative expression of RAGE in lung tissues were detected by Western blot. In
doi_str_mv 10.3760/cma.j.issn.0376-2491.2019.32.015
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