Au@Pd Nanopopcorn and Aptamer Nanoflower Assisted Lateral Flow Strip for Thermal Detection of Exosomes
Conventional lateral flow biosensing technologies face the dual formidable challenges of poor sensitivity and cumbersome quantitative devices. Here, we developed a Au@Pd nanopopcorn and aptamer nanoflower assisted lateral flow strip (ANAN-LFS) with a thermal signal output to improve detection sensit...
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| Veröffentlicht in: | Analytical chemistry (Washington) 2019-11, Vol.91 (21), p.13986-13993 |
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| creator | Cheng, Nan Song, Yang Shi, Qiurong Du, Dan Liu, Dong Luo, Yunbo Xu, Wentao Lin, Yuehe |
| description | Conventional lateral flow biosensing technologies face the dual formidable challenges of poor sensitivity and cumbersome quantitative devices. Here, we developed a Au@Pd nanopopcorn and aptamer nanoflower assisted lateral flow strip (ANAN-LFS) with a thermal signal output to improve detection sensitivity. Moreover, a smartphone-based thermal reader was designed and meticulously optimized to hand-held style, realizing the essential portability of this quantitative device. Experimental studies revealed that the synthesized Au@Pd nanopopcorns clearly red-shifted into the near-infrared region, thus resulting in a higher photothermal response than the standard gold nanoparticles. Aptamer nanoflowers enhanced the system’s biorecognition ability significantly compared with single-stranded aptamers due to their functional spatial structure, thus resulting in an even greater improvement in the sensitivity of the ANAN-LFS. With exosomes as model targets, the limit of detection (LOD) was calculated to be 1.4 × 104 exosomes/μL, which exhibited a 71-fold improved analytical performance. The feasibility of this system for detecting spiked biological samples at clinical concentrations was also confirmed. These results suggest that the proposed strategy of integrating a ANAN-LFS with a smartphone-based thermal reader has great potential as a powerful tool for bioanalytical applications, offering the combined unique advantages of high sensitivity and expedient portability. |
| doi_str_mv | 10.1021/acs.analchem.9b03562 |
| format | Article |
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Here, we developed a Au@Pd nanopopcorn and aptamer nanoflower assisted lateral flow strip (ANAN-LFS) with a thermal signal output to improve detection sensitivity. Moreover, a smartphone-based thermal reader was designed and meticulously optimized to hand-held style, realizing the essential portability of this quantitative device. Experimental studies revealed that the synthesized Au@Pd nanopopcorns clearly red-shifted into the near-infrared region, thus resulting in a higher photothermal response than the standard gold nanoparticles. Aptamer nanoflowers enhanced the system’s biorecognition ability significantly compared with single-stranded aptamers due to their functional spatial structure, thus resulting in an even greater improvement in the sensitivity of the ANAN-LFS. With exosomes as model targets, the limit of detection (LOD) was calculated to be 1.4 × 104 exosomes/μL, which exhibited a 71-fold improved analytical performance. The feasibility of this system for detecting spiked biological samples at clinical concentrations was also confirmed. These results suggest that the proposed strategy of integrating a ANAN-LFS with a smartphone-based thermal reader has great potential as a powerful tool for bioanalytical applications, offering the combined unique advantages of high sensitivity and expedient portability.</description><identifier>ISSN: 0003-2700</identifier><identifier>EISSN: 1520-6882</identifier><identifier>DOI: 10.1021/acs.analchem.9b03562</identifier><identifier>PMID: 31486634</identifier><language>eng</language><publisher>United States: American Chemical Society</publisher><subject>Aptamers ; Biological properties ; Biological samples ; Biosensors ; Chemistry ; Exosomes ; Nanoparticles ; Palladium ; Portability ; Sensitivity ; Smartphones ; Strip ; Target detection</subject><ispartof>Analytical chemistry (Washington), 2019-11, Vol.91 (21), p.13986-13993</ispartof><rights>Copyright American Chemical Society Nov 5, 2019</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-a442t-51acaa28e4d61f3fecab7eb4b1edf846794125a0091b4c002a975063fd9ad05d3</citedby><cites>FETCH-LOGICAL-a442t-51acaa28e4d61f3fecab7eb4b1edf846794125a0091b4c002a975063fd9ad05d3</cites><orcidid>0000-0003-1952-4042 ; 0000-0003-0848-4831 ; 0000-0003-3791-7587 ; 0000-0002-8572-8257</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://pubs.acs.org/doi/pdf/10.1021/acs.analchem.9b03562$$EPDF$$P50$$Gacs$$H</linktopdf><linktohtml>$$Uhttps://pubs.acs.org/doi/10.1021/acs.analchem.9b03562$$EHTML$$P50$$Gacs$$H</linktohtml><link.rule.ids>314,777,781,2752,27057,27905,27906,56719,56769</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/31486634$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Cheng, Nan</creatorcontrib><creatorcontrib>Song, Yang</creatorcontrib><creatorcontrib>Shi, Qiurong</creatorcontrib><creatorcontrib>Du, Dan</creatorcontrib><creatorcontrib>Liu, Dong</creatorcontrib><creatorcontrib>Luo, Yunbo</creatorcontrib><creatorcontrib>Xu, Wentao</creatorcontrib><creatorcontrib>Lin, Yuehe</creatorcontrib><title>Au@Pd Nanopopcorn and Aptamer Nanoflower Assisted Lateral Flow Strip for Thermal Detection of Exosomes</title><title>Analytical chemistry (Washington)</title><addtitle>Anal. Chem</addtitle><description>Conventional lateral flow biosensing technologies face the dual formidable challenges of poor sensitivity and cumbersome quantitative devices. Here, we developed a Au@Pd nanopopcorn and aptamer nanoflower assisted lateral flow strip (ANAN-LFS) with a thermal signal output to improve detection sensitivity. Moreover, a smartphone-based thermal reader was designed and meticulously optimized to hand-held style, realizing the essential portability of this quantitative device. Experimental studies revealed that the synthesized Au@Pd nanopopcorns clearly red-shifted into the near-infrared region, thus resulting in a higher photothermal response than the standard gold nanoparticles. Aptamer nanoflowers enhanced the system’s biorecognition ability significantly compared with single-stranded aptamers due to their functional spatial structure, thus resulting in an even greater improvement in the sensitivity of the ANAN-LFS. With exosomes as model targets, the limit of detection (LOD) was calculated to be 1.4 × 104 exosomes/μL, which exhibited a 71-fold improved analytical performance. The feasibility of this system for detecting spiked biological samples at clinical concentrations was also confirmed. These results suggest that the proposed strategy of integrating a ANAN-LFS with a smartphone-based thermal reader has great potential as a powerful tool for bioanalytical applications, offering the combined unique advantages of high sensitivity and expedient portability.</description><subject>Aptamers</subject><subject>Biological properties</subject><subject>Biological samples</subject><subject>Biosensors</subject><subject>Chemistry</subject><subject>Exosomes</subject><subject>Nanoparticles</subject><subject>Palladium</subject><subject>Portability</subject><subject>Sensitivity</subject><subject>Smartphones</subject><subject>Strip</subject><subject>Target detection</subject><issn>0003-2700</issn><issn>1520-6882</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2019</creationdate><recordtype>article</recordtype><recordid>eNp9kU9v1DAQxS0EokvhGyBkiUsvWcZ_4iQ3VqUFpBWtRDlHE3uspkriYCeCfntcdtsDB04zevq9N9I8xt4K2AqQ4gPatMUJB3tL47bpQJVGPmMbUUooTF3L52wDAKqQFcAJe5XSHYAQIMxLdqKEro1ResP8bv147fg3nMIcZhvixHFyfDcvOFL8q_sh_MrrLqU-LeT4HheKOPDLrPPvS-xn7kPkN7cUxyx_ooXs0oeJB88vfocURkqv2QuPQ6I3x3nKflxe3Jx_KfZXn7-e7_YFai2XohRoEWVN2hnhlSeLXUWd7gQ5X2tTNVrIEgEa0WkLILGpSjDKuwYdlE6dsrND7hzDz5XS0o59sjQMOFFYUytlXQpQRqmMvv8HvQtrzA_NlBKyqioDOlP6QNkYUork2zn2I8b7VkD70EObe2gfe2iPPWTbu2P42o3knkyPj88AHIAH-9Ph_2b-AQ-cl0Q</recordid><startdate>20191105</startdate><enddate>20191105</enddate><creator>Cheng, Nan</creator><creator>Song, Yang</creator><creator>Shi, Qiurong</creator><creator>Du, Dan</creator><creator>Liu, Dong</creator><creator>Luo, Yunbo</creator><creator>Xu, Wentao</creator><creator>Lin, Yuehe</creator><general>American Chemical Society</general><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QF</scope><scope>7QO</scope><scope>7QQ</scope><scope>7SC</scope><scope>7SE</scope><scope>7SP</scope><scope>7SR</scope><scope>7TA</scope><scope>7TB</scope><scope>7TM</scope><scope>7U5</scope><scope>7U7</scope><scope>7U9</scope><scope>8BQ</scope><scope>8FD</scope><scope>C1K</scope><scope>F28</scope><scope>FR3</scope><scope>H8D</scope><scope>H8G</scope><scope>H94</scope><scope>JG9</scope><scope>JQ2</scope><scope>KR7</scope><scope>L7M</scope><scope>L~C</scope><scope>L~D</scope><scope>P64</scope><scope>7X8</scope><orcidid>https://orcid.org/0000-0003-1952-4042</orcidid><orcidid>https://orcid.org/0000-0003-0848-4831</orcidid><orcidid>https://orcid.org/0000-0003-3791-7587</orcidid><orcidid>https://orcid.org/0000-0002-8572-8257</orcidid></search><sort><creationdate>20191105</creationdate><title>Au@Pd Nanopopcorn and Aptamer Nanoflower Assisted Lateral Flow Strip for Thermal Detection of Exosomes</title><author>Cheng, Nan ; Song, Yang ; Shi, Qiurong ; Du, Dan ; Liu, Dong ; Luo, Yunbo ; Xu, Wentao ; Lin, Yuehe</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-a442t-51acaa28e4d61f3fecab7eb4b1edf846794125a0091b4c002a975063fd9ad05d3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2019</creationdate><topic>Aptamers</topic><topic>Biological properties</topic><topic>Biological samples</topic><topic>Biosensors</topic><topic>Chemistry</topic><topic>Exosomes</topic><topic>Nanoparticles</topic><topic>Palladium</topic><topic>Portability</topic><topic>Sensitivity</topic><topic>Smartphones</topic><topic>Strip</topic><topic>Target detection</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Cheng, Nan</creatorcontrib><creatorcontrib>Song, Yang</creatorcontrib><creatorcontrib>Shi, Qiurong</creatorcontrib><creatorcontrib>Du, Dan</creatorcontrib><creatorcontrib>Liu, Dong</creatorcontrib><creatorcontrib>Luo, Yunbo</creatorcontrib><creatorcontrib>Xu, Wentao</creatorcontrib><creatorcontrib>Lin, Yuehe</creatorcontrib><collection>PubMed</collection><collection>CrossRef</collection><collection>Aluminium Industry Abstracts</collection><collection>Biotechnology Research Abstracts</collection><collection>Ceramic Abstracts</collection><collection>Computer and Information Systems Abstracts</collection><collection>Corrosion Abstracts</collection><collection>Electronics & Communications Abstracts</collection><collection>Engineered Materials Abstracts</collection><collection>Materials Business File</collection><collection>Mechanical & Transportation Engineering Abstracts</collection><collection>Nucleic Acids Abstracts</collection><collection>Solid State and Superconductivity Abstracts</collection><collection>Toxicology Abstracts</collection><collection>Virology and AIDS Abstracts</collection><collection>METADEX</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>ANTE: Abstracts in New Technology & Engineering</collection><collection>Engineering Research Database</collection><collection>Aerospace Database</collection><collection>Copper Technical Reference Library</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>Materials Research Database</collection><collection>ProQuest Computer Science Collection</collection><collection>Civil Engineering Abstracts</collection><collection>Advanced Technologies Database with Aerospace</collection><collection>Computer and Information Systems Abstracts Academic</collection><collection>Computer and Information Systems Abstracts Professional</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Analytical chemistry (Washington)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Cheng, Nan</au><au>Song, Yang</au><au>Shi, Qiurong</au><au>Du, Dan</au><au>Liu, Dong</au><au>Luo, Yunbo</au><au>Xu, Wentao</au><au>Lin, Yuehe</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Au@Pd Nanopopcorn and Aptamer Nanoflower Assisted Lateral Flow Strip for Thermal Detection of Exosomes</atitle><jtitle>Analytical chemistry (Washington)</jtitle><addtitle>Anal. Chem</addtitle><date>2019-11-05</date><risdate>2019</risdate><volume>91</volume><issue>21</issue><spage>13986</spage><epage>13993</epage><pages>13986-13993</pages><issn>0003-2700</issn><eissn>1520-6882</eissn><abstract>Conventional lateral flow biosensing technologies face the dual formidable challenges of poor sensitivity and cumbersome quantitative devices. Here, we developed a Au@Pd nanopopcorn and aptamer nanoflower assisted lateral flow strip (ANAN-LFS) with a thermal signal output to improve detection sensitivity. Moreover, a smartphone-based thermal reader was designed and meticulously optimized to hand-held style, realizing the essential portability of this quantitative device. Experimental studies revealed that the synthesized Au@Pd nanopopcorns clearly red-shifted into the near-infrared region, thus resulting in a higher photothermal response than the standard gold nanoparticles. 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| source | ACS Publications |
| subjects | Aptamers Biological properties Biological samples Biosensors Chemistry Exosomes Nanoparticles Palladium Portability Sensitivity Smartphones Strip Target detection |
| title | Au@Pd Nanopopcorn and Aptamer Nanoflower Assisted Lateral Flow Strip for Thermal Detection of Exosomes |
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