Preparation and characterization of hybrid chitosan-silver nanoparticles (Chi-Ag NPs); A potential antibacterial agent

A graphic abstract representing the key steps involved the green synthesis of hybrid Chi-Ag NPs from Talinum portulacifolium. Talinum portulacifolium leaf extracts were acting as a reducing agent to reduce the Ag ions with addition of chitosan to form hybrid Chi-Ag NPs. [Display omitted] •Hybrid chitosan-silver nanoparticles were prepared using leaf extract of Talinum portulacifolium.•XRD pattern revealed that hybrid Chi-Ag NPs have a crystalline and face-centered cubic configuration.•TEM image shows that the prepared hybrid Chi-Ag NPs were spherical in shape with an average particles size various from 3.24 nm to 44.80 nm.•FESEM images explains the interaction of hybrid Chi-Ag NPs with the surface membrane of E. coli and S. marcescens and its antibacterial activity. In this study, a novel ecofriendly chitosan- silver nanoparticles hybrid was developed. Biological method using leaf extract of T. portulacifolium was used as reducing agent for its synthesis and the antibacterial efficiency of these hybrid nanoparticles were evaluated against the bacteria E. coli and S. marcescens organisms. The intense peak observed around 419 nm in the UV–Vis indicates the formation of silver nanoparticles. The XRD analysis showed that the hybrid chitosan-silver nanoparticles have a polycrystalline and face-centered cubic configuration. FTIR spectrum hybrid chitosan-silver nanoparticles indicated speaks vibration of NH and OH. The EDS analysis confirmed the presence of Ag, O, C and N elements in the prepared sample. The spherical shape was obtained from TEM analysis and it indicated that with average particles around 3.24 nm to 44.80 nm. The prepared hybrid chitosan-silver nanoparticles showed significant antibacterial activities against E. coli and S. marcescens. In addition, the surface membrane damages and surface morphology of test pathogens were visualized using FESEM analysis.