Effect of heat stress and Hsp90 inhibition on T-type calcium currents and voltage-dependent potassium currents in leydig cells
Heat can trigger testicular damage and impair fertility. Leydig cells produce testosterone in response to stimulation by luteinizing hormone (LH), which induces Ca2+ entry and K+ efflux through ion channels in their plasma membrane. Considering that mechanisms coordinating the Leydig cell responses...
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| Veröffentlicht in: | Journal of thermal biology 2019-08, Vol.84, p.1-7 |
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| container_title | Journal of thermal biology |
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| creator | Tenorio, Bruno Mendes Pereira da Silva, Reginaldo Tenorio, Fernanda das Chagas Angelo Mendes Costa Rosales, Roberta Ribeiro Amaro da Silva Junior, Valdemiro de Albuquerque Nogueira, Romildo |
| description | Heat can trigger testicular damage and impair fertility. Leydig cells produce testosterone in response to stimulation by luteinizing hormone (LH), which induces Ca2+ entry and K+ efflux through ion channels in their plasma membrane. Considering that mechanisms coordinating the Leydig cell responses to hyperthermic stress remain unclear; the present study analyzed the effects of heat stress (HS, 43°C, 15 min) and inhibition of Hsp90 on T-type calcium currents and voltage-dependent potassium currents (VKC) in mice Leydig cells. Results show that HS reduced the VKC steady state currents at +80 mV (45.3%) and maximum conductance (71.5%), as well as increased the activation time constant (31.7%) and the voltage for which half the channels are open (30%). Hsp90 inhibition did not change the VKC currents. T-type calcium currents were not affected by HS or Hsp90 inhibition. In conclusion, HS can slow the activation, reduce the currents and voltage dependence of the VKC, suggesting a possible role of these currents in the response to hyperthermic stress in Leydig cells.
[Display omitted]
•Effect of heat stress and Hsp90 inhibition on K+ and Ca2+ currents in Leydig cells.•Heat stress reduced voltage-dependent K+ currents.•T-type Ca2+ currents did not change after heat stress.•Hsp90 inhibition did not change the K+ and Ca2+ currents. |
| doi_str_mv | 10.1016/j.jtherbio.2019.05.022 |
| format | Article |
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[Display omitted]
•Effect of heat stress and Hsp90 inhibition on K+ and Ca2+ currents in Leydig cells.•Heat stress reduced voltage-dependent K+ currents.•T-type Ca2+ currents did not change after heat stress.•Hsp90 inhibition did not change the K+ and Ca2+ currents.</description><identifier>ISSN: 0306-4565</identifier><identifier>EISSN: 1879-0992</identifier><identifier>DOI: 10.1016/j.jtherbio.2019.05.022</identifier><identifier>PMID: 31466741</identifier><language>eng</language><publisher>England: Elsevier Ltd</publisher><subject>Animals ; Benzoquinones - pharmacology ; Calcium channels (T-type) ; Calcium channels (voltage-gated) ; Calcium Channels, T-Type - physiology ; Calcium currents ; Calcium efflux ; Calcium influx ; Conductance ; Fertility ; Heat stress ; Heat-Shock Response - physiology ; High temperature ; Hot Temperature ; Hsp90 ; HSP90 Heat-Shock Proteins - antagonists & inhibitors ; HSP90 Heat-Shock Proteins - physiology ; Hsp90 protein ; Ion channels ; Lactams, Macrocyclic - pharmacology ; Leydig cells ; Leydig Cells - drug effects ; Leydig Cells - physiology ; Luteinizing hormone ; Male ; Menopause ; Mice ; Potassium ; Potassium channels (voltage-gated) ; Potassium Channels, Voltage-Gated - physiology ; Potassium currents ; Testosterone</subject><ispartof>Journal of thermal biology, 2019-08, Vol.84, p.1-7</ispartof><rights>2019 Elsevier Ltd</rights><rights>Copyright © 2019 Elsevier Ltd. All rights reserved.</rights><rights>Copyright Elsevier BV Aug 2019</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c396t-52f409d994b1f6d45da6187430962b060a9a97ad49e9e2069d49397a168f6fab3</citedby><cites>FETCH-LOGICAL-c396t-52f409d994b1f6d45da6187430962b060a9a97ad49e9e2069d49397a168f6fab3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://www.sciencedirect.com/science/article/pii/S0306456518304285$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,776,780,3537,27901,27902,65306</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/31466741$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Tenorio, Bruno Mendes</creatorcontrib><creatorcontrib>Pereira da Silva, Reginaldo</creatorcontrib><creatorcontrib>Tenorio, Fernanda das Chagas Angelo Mendes</creatorcontrib><creatorcontrib>Costa Rosales, Roberta Ribeiro</creatorcontrib><creatorcontrib>Amaro da Silva Junior, Valdemiro</creatorcontrib><creatorcontrib>de Albuquerque Nogueira, Romildo</creatorcontrib><title>Effect of heat stress and Hsp90 inhibition on T-type calcium currents and voltage-dependent potassium currents in leydig cells</title><title>Journal of thermal biology</title><addtitle>J Therm Biol</addtitle><description>Heat can trigger testicular damage and impair fertility. Leydig cells produce testosterone in response to stimulation by luteinizing hormone (LH), which induces Ca2+ entry and K+ efflux through ion channels in their plasma membrane. Considering that mechanisms coordinating the Leydig cell responses to hyperthermic stress remain unclear; the present study analyzed the effects of heat stress (HS, 43°C, 15 min) and inhibition of Hsp90 on T-type calcium currents and voltage-dependent potassium currents (VKC) in mice Leydig cells. Results show that HS reduced the VKC steady state currents at +80 mV (45.3%) and maximum conductance (71.5%), as well as increased the activation time constant (31.7%) and the voltage for which half the channels are open (30%). Hsp90 inhibition did not change the VKC currents. T-type calcium currents were not affected by HS or Hsp90 inhibition. In conclusion, HS can slow the activation, reduce the currents and voltage dependence of the VKC, suggesting a possible role of these currents in the response to hyperthermic stress in Leydig cells.
[Display omitted]
•Effect of heat stress and Hsp90 inhibition on K+ and Ca2+ currents in Leydig cells.•Heat stress reduced voltage-dependent K+ currents.•T-type Ca2+ currents did not change after heat stress.•Hsp90 inhibition did not change the K+ and Ca2+ currents.</description><subject>Animals</subject><subject>Benzoquinones - pharmacology</subject><subject>Calcium channels (T-type)</subject><subject>Calcium channels (voltage-gated)</subject><subject>Calcium Channels, T-Type - physiology</subject><subject>Calcium currents</subject><subject>Calcium efflux</subject><subject>Calcium influx</subject><subject>Conductance</subject><subject>Fertility</subject><subject>Heat stress</subject><subject>Heat-Shock Response - physiology</subject><subject>High temperature</subject><subject>Hot Temperature</subject><subject>Hsp90</subject><subject>HSP90 Heat-Shock Proteins - antagonists & inhibitors</subject><subject>HSP90 Heat-Shock Proteins - physiology</subject><subject>Hsp90 protein</subject><subject>Ion channels</subject><subject>Lactams, Macrocyclic - pharmacology</subject><subject>Leydig cells</subject><subject>Leydig Cells - drug effects</subject><subject>Leydig Cells - physiology</subject><subject>Luteinizing hormone</subject><subject>Male</subject><subject>Menopause</subject><subject>Mice</subject><subject>Potassium</subject><subject>Potassium channels (voltage-gated)</subject><subject>Potassium Channels, Voltage-Gated - physiology</subject><subject>Potassium currents</subject><subject>Testosterone</subject><issn>0306-4565</issn><issn>1879-0992</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2019</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkc9rFDEUgIModq3-CyXgxcuML8lMdnNTSrVCwUs9h0zy0s0wOxmTTGEv_u1m2VbQixBIeHzvR95HyBWDlgGTH8d2LHtMQ4gtB6Za6Fvg_AXZsN1WNaAUf0k2IEA2XS_7C_Im5xGA9aKH1-RCsE7Kbcc25NeN92gLjZ7u0RSaS8KcqZkdvc2LAhrmfRhCCXGm9dw35bggtWayYT1Qu6aEcznzj3Eq5gEbhwvOrobpEovJ-S8wzHTCowsP1OI05bfklTdTxndP9yX58eXm_vq2ufv-9dv157vGCiVL03PfgXJKdQPz0nW9M7J-tBOgJB9AglFGbY3rFCrkIFV9iRpgcuelN4O4JB_OdZcUf66Yiz6EfJrAzBjXrDnfCcY4E6qi7_9Bx7imuU5XKSVl3acSlZJnyqaYc0KvlxQOJh01A30ypEf9bEifDGnodTVUE6-eyq_DAd2ftGclFfh0BrDu4zFg0tkGnC26kKop7WL4X4_fxLClzg</recordid><startdate>201908</startdate><enddate>201908</enddate><creator>Tenorio, Bruno Mendes</creator><creator>Pereira da Silva, Reginaldo</creator><creator>Tenorio, Fernanda das Chagas Angelo Mendes</creator><creator>Costa Rosales, Roberta Ribeiro</creator><creator>Amaro da Silva Junior, Valdemiro</creator><creator>de Albuquerque Nogueira, Romildo</creator><general>Elsevier Ltd</general><general>Elsevier BV</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QG</scope><scope>7QP</scope><scope>7SS</scope><scope>7TK</scope><scope>7TS</scope><scope>7X8</scope></search><sort><creationdate>201908</creationdate><title>Effect of heat stress and Hsp90 inhibition on T-type calcium currents and voltage-dependent potassium currents in leydig cells</title><author>Tenorio, Bruno Mendes ; Pereira da Silva, Reginaldo ; Tenorio, Fernanda das Chagas Angelo Mendes ; Costa Rosales, Roberta Ribeiro ; Amaro da Silva Junior, Valdemiro ; de Albuquerque Nogueira, Romildo</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c396t-52f409d994b1f6d45da6187430962b060a9a97ad49e9e2069d49397a168f6fab3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2019</creationdate><topic>Animals</topic><topic>Benzoquinones - pharmacology</topic><topic>Calcium channels (T-type)</topic><topic>Calcium channels (voltage-gated)</topic><topic>Calcium Channels, T-Type - physiology</topic><topic>Calcium currents</topic><topic>Calcium efflux</topic><topic>Calcium influx</topic><topic>Conductance</topic><topic>Fertility</topic><topic>Heat stress</topic><topic>Heat-Shock Response - physiology</topic><topic>High temperature</topic><topic>Hot Temperature</topic><topic>Hsp90</topic><topic>HSP90 Heat-Shock Proteins - antagonists & inhibitors</topic><topic>HSP90 Heat-Shock Proteins - physiology</topic><topic>Hsp90 protein</topic><topic>Ion channels</topic><topic>Lactams, Macrocyclic - pharmacology</topic><topic>Leydig cells</topic><topic>Leydig Cells - drug effects</topic><topic>Leydig Cells - physiology</topic><topic>Luteinizing hormone</topic><topic>Male</topic><topic>Menopause</topic><topic>Mice</topic><topic>Potassium</topic><topic>Potassium channels (voltage-gated)</topic><topic>Potassium Channels, Voltage-Gated - physiology</topic><topic>Potassium currents</topic><topic>Testosterone</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Tenorio, Bruno Mendes</creatorcontrib><creatorcontrib>Pereira da Silva, Reginaldo</creatorcontrib><creatorcontrib>Tenorio, Fernanda das Chagas Angelo Mendes</creatorcontrib><creatorcontrib>Costa Rosales, Roberta Ribeiro</creatorcontrib><creatorcontrib>Amaro da Silva Junior, Valdemiro</creatorcontrib><creatorcontrib>de Albuquerque Nogueira, Romildo</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Animal Behavior Abstracts</collection><collection>Calcium & Calcified Tissue Abstracts</collection><collection>Entomology Abstracts (Full archive)</collection><collection>Neurosciences Abstracts</collection><collection>Physical Education Index</collection><collection>MEDLINE - Academic</collection><jtitle>Journal of thermal biology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Tenorio, Bruno Mendes</au><au>Pereira da Silva, Reginaldo</au><au>Tenorio, Fernanda das Chagas Angelo Mendes</au><au>Costa Rosales, Roberta Ribeiro</au><au>Amaro da Silva Junior, Valdemiro</au><au>de Albuquerque Nogueira, Romildo</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Effect of heat stress and Hsp90 inhibition on T-type calcium currents and voltage-dependent potassium currents in leydig cells</atitle><jtitle>Journal of thermal biology</jtitle><addtitle>J Therm Biol</addtitle><date>2019-08</date><risdate>2019</risdate><volume>84</volume><spage>1</spage><epage>7</epage><pages>1-7</pages><issn>0306-4565</issn><eissn>1879-0992</eissn><abstract>Heat can trigger testicular damage and impair fertility. Leydig cells produce testosterone in response to stimulation by luteinizing hormone (LH), which induces Ca2+ entry and K+ efflux through ion channels in their plasma membrane. Considering that mechanisms coordinating the Leydig cell responses to hyperthermic stress remain unclear; the present study analyzed the effects of heat stress (HS, 43°C, 15 min) and inhibition of Hsp90 on T-type calcium currents and voltage-dependent potassium currents (VKC) in mice Leydig cells. Results show that HS reduced the VKC steady state currents at +80 mV (45.3%) and maximum conductance (71.5%), as well as increased the activation time constant (31.7%) and the voltage for which half the channels are open (30%). Hsp90 inhibition did not change the VKC currents. T-type calcium currents were not affected by HS or Hsp90 inhibition. In conclusion, HS can slow the activation, reduce the currents and voltage dependence of the VKC, suggesting a possible role of these currents in the response to hyperthermic stress in Leydig cells.
[Display omitted]
•Effect of heat stress and Hsp90 inhibition on K+ and Ca2+ currents in Leydig cells.•Heat stress reduced voltage-dependent K+ currents.•T-type Ca2+ currents did not change after heat stress.•Hsp90 inhibition did not change the K+ and Ca2+ currents.</abstract><cop>England</cop><pub>Elsevier Ltd</pub><pmid>31466741</pmid><doi>10.1016/j.jtherbio.2019.05.022</doi><tpages>7</tpages></addata></record> |
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| subjects | Animals Benzoquinones - pharmacology Calcium channels (T-type) Calcium channels (voltage-gated) Calcium Channels, T-Type - physiology Calcium currents Calcium efflux Calcium influx Conductance Fertility Heat stress Heat-Shock Response - physiology High temperature Hot Temperature Hsp90 HSP90 Heat-Shock Proteins - antagonists & inhibitors HSP90 Heat-Shock Proteins - physiology Hsp90 protein Ion channels Lactams, Macrocyclic - pharmacology Leydig cells Leydig Cells - drug effects Leydig Cells - physiology Luteinizing hormone Male Menopause Mice Potassium Potassium channels (voltage-gated) Potassium Channels, Voltage-Gated - physiology Potassium currents Testosterone |
| title | Effect of heat stress and Hsp90 inhibition on T-type calcium currents and voltage-dependent potassium currents in leydig cells |
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