Morphometric parameters of foodborne related-pathogens estimated by transmission electron microscopy and their relation to optical density and colony forming units
The different morphological characteristics of five bacterial pathogen strains were analyzed through transmission electron microscopy for addressing the particular relationship between optical density and colony-forming units for each strain. Generated linear equations will allow a reliable calculat...
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Veröffentlicht in: | Journal of microbiological methods 2019-10, Vol.165, p.105691-105691, Article 105691 |
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container_title | Journal of microbiological methods |
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creator | González-Pérez, C.J. Tanori-Cordova, J. Aispuro-Hernández, E. Vargas-Arispuro, I. Martínez-Téllez, M.A. |
description | The different morphological characteristics of five bacterial pathogen strains were analyzed through transmission electron microscopy for addressing the particular relationship between optical density and colony-forming units for each strain. Generated linear equations will allow a reliable calculation of bacterial concentrations through simple optical density measurements.
•Morphology is related to bacterial concentrations obtained by optical density.•Equations using optical densities can predict the number of colony-forming units.•The bigger the bacterial size, the lower the slope in the linear equation. |
doi_str_mv | 10.1016/j.mimet.2019.105691 |
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•Morphology is related to bacterial concentrations obtained by optical density.•Equations using optical densities can predict the number of colony-forming units.•The bigger the bacterial size, the lower the slope in the linear equation.</description><identifier>ISSN: 0167-7012</identifier><identifier>EISSN: 1872-8359</identifier><identifier>DOI: 10.1016/j.mimet.2019.105691</identifier><identifier>PMID: 31437554</identifier><language>eng</language><publisher>Netherlands: Elsevier B.V</publisher><subject>Bacterial cell morphology ; CFU·mL−1 calculation ; Escherichia coli O157:H7 ; Listeria monocytogenes ; Salmonella Saintpaul ; Salmonella Typhimurium ; Staphylococcus aureus ; Transmission electron microscopy</subject><ispartof>Journal of microbiological methods, 2019-10, Vol.165, p.105691-105691, Article 105691</ispartof><rights>2019 Elsevier B.V.</rights><rights>Copyright © 2019 Elsevier B.V. All rights reserved.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c359t-f056ca4c6e5a1f709c6b6b07c89f395d3ff2265d0340e49fc9206d07f4160a613</citedby><cites>FETCH-LOGICAL-c359t-f056ca4c6e5a1f709c6b6b07c89f395d3ff2265d0340e49fc9206d07f4160a613</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/j.mimet.2019.105691$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,777,781,3537,27905,27906,45976</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/31437554$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>González-Pérez, C.J.</creatorcontrib><creatorcontrib>Tanori-Cordova, J.</creatorcontrib><creatorcontrib>Aispuro-Hernández, E.</creatorcontrib><creatorcontrib>Vargas-Arispuro, I.</creatorcontrib><creatorcontrib>Martínez-Téllez, M.A.</creatorcontrib><title>Morphometric parameters of foodborne related-pathogens estimated by transmission electron microscopy and their relation to optical density and colony forming units</title><title>Journal of microbiological methods</title><addtitle>J Microbiol Methods</addtitle><description>The different morphological characteristics of five bacterial pathogen strains were analyzed through transmission electron microscopy for addressing the particular relationship between optical density and colony-forming units for each strain. Generated linear equations will allow a reliable calculation of bacterial concentrations through simple optical density measurements.
•Morphology is related to bacterial concentrations obtained by optical density.•Equations using optical densities can predict the number of colony-forming units.•The bigger the bacterial size, the lower the slope in the linear equation.</description><subject>Bacterial cell morphology</subject><subject>CFU·mL−1 calculation</subject><subject>Escherichia coli O157:H7</subject><subject>Listeria monocytogenes</subject><subject>Salmonella Saintpaul</subject><subject>Salmonella Typhimurium</subject><subject>Staphylococcus aureus</subject><subject>Transmission electron microscopy</subject><issn>0167-7012</issn><issn>1872-8359</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2019</creationdate><recordtype>article</recordtype><recordid>eNp9kc9u1DAQxi0EokvLEyAhH7lk8Z_EiQ8cUEVLpSIu9Gw59rjrVWIH24u0z8OL4pDCsacZjX7zjb75EHpHyZ4SKj4e97OfoewZobJOOiHpC7SjQ8-agXfyJdpVqm96QtkFepPzkRDa8XZ4jS44bXnfde0O_f4W03KIVSd5gxeddG0hZRwddjHaMaYAOMGkC9hm0eUQHyFkDLn4eZ3h8YxL0iHPPmcfA4YJTEm1mb1JMZu4nLEOFpcD-LQprViJOC7FGz1hWwV92SgTpxjO9XSafXjEp-BLvkKvnJ4yvH2ql-jh5suP66_N_ffbu-vP942pdkvj6guMbo2ATlPXE2nEKEbSm0E6LjvLnWNMdJbwlkArnZGMCEt611JBtKD8En3YdJcUf56qQ1U9GZgmHSCesmJs4JQMXLKK8g1dLeYETi2p_iOdFSVqTUcd1d901JqO2tKpW--fDpzGGez_nX9xVODTBkC1-ctDUtl4CAasT_Wrykb_7IE_cPOmmw</recordid><startdate>201910</startdate><enddate>201910</enddate><creator>González-Pérez, C.J.</creator><creator>Tanori-Cordova, J.</creator><creator>Aispuro-Hernández, E.</creator><creator>Vargas-Arispuro, I.</creator><creator>Martínez-Téllez, M.A.</creator><general>Elsevier B.V</general><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>201910</creationdate><title>Morphometric parameters of foodborne related-pathogens estimated by transmission electron microscopy and their relation to optical density and colony forming units</title><author>González-Pérez, C.J. ; Tanori-Cordova, J. ; Aispuro-Hernández, E. ; Vargas-Arispuro, I. ; Martínez-Téllez, M.A.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c359t-f056ca4c6e5a1f709c6b6b07c89f395d3ff2265d0340e49fc9206d07f4160a613</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2019</creationdate><topic>Bacterial cell morphology</topic><topic>CFU·mL−1 calculation</topic><topic>Escherichia coli O157:H7</topic><topic>Listeria monocytogenes</topic><topic>Salmonella Saintpaul</topic><topic>Salmonella Typhimurium</topic><topic>Staphylococcus aureus</topic><topic>Transmission electron microscopy</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>González-Pérez, C.J.</creatorcontrib><creatorcontrib>Tanori-Cordova, J.</creatorcontrib><creatorcontrib>Aispuro-Hernández, E.</creatorcontrib><creatorcontrib>Vargas-Arispuro, I.</creatorcontrib><creatorcontrib>Martínez-Téllez, M.A.</creatorcontrib><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Journal of microbiological methods</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>González-Pérez, C.J.</au><au>Tanori-Cordova, J.</au><au>Aispuro-Hernández, E.</au><au>Vargas-Arispuro, I.</au><au>Martínez-Téllez, M.A.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Morphometric parameters of foodborne related-pathogens estimated by transmission electron microscopy and their relation to optical density and colony forming units</atitle><jtitle>Journal of microbiological methods</jtitle><addtitle>J Microbiol Methods</addtitle><date>2019-10</date><risdate>2019</risdate><volume>165</volume><spage>105691</spage><epage>105691</epage><pages>105691-105691</pages><artnum>105691</artnum><issn>0167-7012</issn><eissn>1872-8359</eissn><abstract>The different morphological characteristics of five bacterial pathogen strains were analyzed through transmission electron microscopy for addressing the particular relationship between optical density and colony-forming units for each strain. Generated linear equations will allow a reliable calculation of bacterial concentrations through simple optical density measurements.
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subjects | Bacterial cell morphology CFU·mL−1 calculation Escherichia coli O157:H7 Listeria monocytogenes Salmonella Saintpaul Salmonella Typhimurium Staphylococcus aureus Transmission electron microscopy |
title | Morphometric parameters of foodborne related-pathogens estimated by transmission electron microscopy and their relation to optical density and colony forming units |
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