CRISPR-Cas experiments for schools and the public

Graphical summary of CRISPR-Cas experiments for schools and the public – CRISPR-Cas mediated lacZ knock out in E. coli. Plasmid borne lacZ is targeted to convert blue E. coli to white E. coli. (A) Cells transformed with lacZ containing plasmid pKW5 turn blue in the presence of the chromogenic substr...

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Veröffentlicht in:	Methods (San Diego, Calif.) Calif.), 2020-02, Vol.172, p.86-94
Hauptverfasser:	Ziegler, Heike, Nellen, Wolfgang
Format:	Artikel
Sprache:	eng
Schlagworte:	Adolescent Adult Aged beta-Galactosidase - genetics beta-Galactosidase - metabolism Cas9 Chromogenic Compounds - metabolism Class room experiment Color CRISPR CRISPR-Cas Systems - genetics DNA Breaks, Double-Stranded E. coli Education - methods Educational module Escherichia coli - enzymology Escherichia coli - genetics Escherichia coli Proteins - genetics Escherichia coli Proteins - metabolism Galactosides - metabolism Gene Editing - methods Humans Indoles - metabolism Lac Operon - genetics lacZ Middle Aged Molecular Biology - education Plasmids - genetics Schools Students Young Adult
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