Early Detection of Localized Immunity in Experimental Autoimmune Myocarditis Using [99mTc]Fucoidan SPECT
Purpose The aim of the study was to evaluate the ability of technetium-99m-fucoidan ([ 99m Tc]fucoidan), a molecular imaging agent specific for selectins, in the assessment of early localized immunity in a rat model of experimental autoimmune myocarditis (EAM). Procedures EAM was induced in Lewis ra...
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| Veröffentlicht in: | Molecular imaging and biology 2020-06, Vol.22 (3), p.643-652 |
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| creator | Vigne, Jonathan Cognet, Thomas Guedj, Kevin Morvan, Marion Merceron, Olivier Louedec, Liliane Choqueux, Christine Nicoletti, Antonino Escoubet, Brigitte Chaubet, Frederic Michel, Jean-Baptiste Rouzet, François |
| description | Purpose
The aim of the study was to evaluate the ability of technetium-99m-fucoidan ([
99m
Tc]fucoidan), a molecular imaging agent specific for selectins, in the assessment of early localized immunity in a rat model of experimental autoimmune myocarditis (EAM).
Procedures
EAM was induced in Lewis rats and troponin T; brain natriuretic peptide (BNP) and anti-myosin antibodies were measured in plasma. Separately, [
99m
Tc]fucoidan single-photon emission computed tomography (SPECT)/x-ray computed tomography (CT) was performed in the very early phase of myocarditis at 10, 15, and 21 days after immunization. Then, hearts were collected and used for autoradiography, well counting, histology, and flow cytometry analysis.
Results
The EAM acute phase is characterized by extensive myocardial necrosis, release of troponin and BNP, and pericardial effusion. [
99m
Tc]Fucoidan uptake was significantly increased in EAM compared with controls starting from D15. There was a close relationship between uptake of the tracer and myocardial content in CD45+, CD8+, CD11b+, and CD31+ cells.
Conclusions
[
99m
Tc]Fucoidan SPECT/CT accurately diagnosed the autoimmune attack in the early steps of EAM and could be used to monitor disease evolution and therapy efficiency. |
| doi_str_mv | 10.1007/s11307-019-01420-8 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_2282494891</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>2276693237</sourcerecordid><originalsourceid>FETCH-LOGICAL-c403t-3c673fe33fbda357a4797d2d16a9394781de6d8fc0bf6693f45b84c51caff7b43</originalsourceid><addsrcrecordid>eNp9kUuLFDEURoMozkP_gAsJuHFTmlflsRzannGgRcGelUhI5TFmqErapApsf71pe1RwMYuQwD33u5ccAF5g9AYjJN5WjCkSHcKqHUZQJx-BUyw56ghC5HF795R3mFNyAs5qvUMIC0zoU3BCMaOESnUKvq1NGffwnZ-9nWNOMAe4ydaM8ad38HqalhTnPYwJrn_sfImTT7MZ4cUy53goevhh3_Di4hwrvKkx3cIvSk1b-_VysTk6k-DnT-vV9hl4EsxY_fP7-xzcXK63q_fd5uPV9epi01mG6NxRywUNntIwOEN7YZhQwhGHuVFUMSGx89zJYNEQOFc0sH6QzPbYmhDEwOg5eH3M3ZX8ffF11lOs1o-jST4vVRMiCVNMKtzQV_-hd3kpqW2nCUNc8V4y8iBFxGEHQkWjyJGyJddafNC79lmm7DVG-mBLH23pZkv_tqVla3p5H70Mk3d_W_7oaQA9ArWV0q0v_2Y_EPsLT02egA</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>2276693237</pqid></control><display><type>article</type><title>Early Detection of Localized Immunity in Experimental Autoimmune Myocarditis Using [99mTc]Fucoidan SPECT</title><source>SpringerLink Journals</source><creator>Vigne, Jonathan ; Cognet, Thomas ; Guedj, Kevin ; Morvan, Marion ; Merceron, Olivier ; Louedec, Liliane ; Choqueux, Christine ; Nicoletti, Antonino ; Escoubet, Brigitte ; Chaubet, Frederic ; Michel, Jean-Baptiste ; Rouzet, François</creator><creatorcontrib>Vigne, Jonathan ; Cognet, Thomas ; Guedj, Kevin ; Morvan, Marion ; Merceron, Olivier ; Louedec, Liliane ; Choqueux, Christine ; Nicoletti, Antonino ; Escoubet, Brigitte ; Chaubet, Frederic ; Michel, Jean-Baptiste ; Rouzet, François</creatorcontrib><description>Purpose
The aim of the study was to evaluate the ability of technetium-99m-fucoidan ([
99m
Tc]fucoidan), a molecular imaging agent specific for selectins, in the assessment of early localized immunity in a rat model of experimental autoimmune myocarditis (EAM).
Procedures
EAM was induced in Lewis rats and troponin T; brain natriuretic peptide (BNP) and anti-myosin antibodies were measured in plasma. Separately, [
99m
Tc]fucoidan single-photon emission computed tomography (SPECT)/x-ray computed tomography (CT) was performed in the very early phase of myocarditis at 10, 15, and 21 days after immunization. Then, hearts were collected and used for autoradiography, well counting, histology, and flow cytometry analysis.
Results
The EAM acute phase is characterized by extensive myocardial necrosis, release of troponin and BNP, and pericardial effusion. [
99m
Tc]Fucoidan uptake was significantly increased in EAM compared with controls starting from D15. There was a close relationship between uptake of the tracer and myocardial content in CD45+, CD8+, CD11b+, and CD31+ cells.
Conclusions
[
99m
Tc]Fucoidan SPECT/CT accurately diagnosed the autoimmune attack in the early steps of EAM and could be used to monitor disease evolution and therapy efficiency.</description><identifier>ISSN: 1536-1632</identifier><identifier>EISSN: 1860-2002</identifier><identifier>DOI: 10.1007/s11307-019-01420-8</identifier><identifier>PMID: 31432389</identifier><language>eng</language><publisher>Cham: Springer International Publishing</publisher><subject>Antibodies ; Autoradiography ; Brain ; Brain natriuretic peptide ; Calcium-binding protein ; CD11b antigen ; CD45 antigen ; CD8 antigen ; Computation ; Computed tomography ; Effusion ; Flow cytometry ; Fucoidan ; Heart diseases ; Histology ; Imaging ; Immunity ; Immunization ; Medicine ; Medicine & Public Health ; Myocarditis ; Myosin ; Necrosis ; Neuroimaging ; Photon emission ; Radiology ; Research Article ; Selectins ; Single photon emission computed tomography ; Technetium ; Technetium isotopes ; Tomography ; Troponin ; Troponin T</subject><ispartof>Molecular imaging and biology, 2020-06, Vol.22 (3), p.643-652</ispartof><rights>World Molecular Imaging Society 2019</rights><rights>Molecular Imaging and Biology is a copyright of Springer, (2019). All Rights Reserved.</rights><rights>World Molecular Imaging Society 2019.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c403t-3c673fe33fbda357a4797d2d16a9394781de6d8fc0bf6693f45b84c51caff7b43</citedby><cites>FETCH-LOGICAL-c403t-3c673fe33fbda357a4797d2d16a9394781de6d8fc0bf6693f45b84c51caff7b43</cites><orcidid>0000-0002-6575-9224</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://link.springer.com/content/pdf/10.1007/s11307-019-01420-8$$EPDF$$P50$$Gspringer$$H</linktopdf><linktohtml>$$Uhttps://link.springer.com/10.1007/s11307-019-01420-8$$EHTML$$P50$$Gspringer$$H</linktohtml><link.rule.ids>314,777,781,27905,27906,41469,42538,51300</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/31432389$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Vigne, Jonathan</creatorcontrib><creatorcontrib>Cognet, Thomas</creatorcontrib><creatorcontrib>Guedj, Kevin</creatorcontrib><creatorcontrib>Morvan, Marion</creatorcontrib><creatorcontrib>Merceron, Olivier</creatorcontrib><creatorcontrib>Louedec, Liliane</creatorcontrib><creatorcontrib>Choqueux, Christine</creatorcontrib><creatorcontrib>Nicoletti, Antonino</creatorcontrib><creatorcontrib>Escoubet, Brigitte</creatorcontrib><creatorcontrib>Chaubet, Frederic</creatorcontrib><creatorcontrib>Michel, Jean-Baptiste</creatorcontrib><creatorcontrib>Rouzet, François</creatorcontrib><title>Early Detection of Localized Immunity in Experimental Autoimmune Myocarditis Using [99mTc]Fucoidan SPECT</title><title>Molecular imaging and biology</title><addtitle>Mol Imaging Biol</addtitle><addtitle>Mol Imaging Biol</addtitle><description>Purpose
The aim of the study was to evaluate the ability of technetium-99m-fucoidan ([
99m
Tc]fucoidan), a molecular imaging agent specific for selectins, in the assessment of early localized immunity in a rat model of experimental autoimmune myocarditis (EAM).
Procedures
EAM was induced in Lewis rats and troponin T; brain natriuretic peptide (BNP) and anti-myosin antibodies were measured in plasma. Separately, [
99m
Tc]fucoidan single-photon emission computed tomography (SPECT)/x-ray computed tomography (CT) was performed in the very early phase of myocarditis at 10, 15, and 21 days after immunization. Then, hearts were collected and used for autoradiography, well counting, histology, and flow cytometry analysis.
Results
The EAM acute phase is characterized by extensive myocardial necrosis, release of troponin and BNP, and pericardial effusion. [
99m
Tc]Fucoidan uptake was significantly increased in EAM compared with controls starting from D15. There was a close relationship between uptake of the tracer and myocardial content in CD45+, CD8+, CD11b+, and CD31+ cells.
Conclusions
[
99m
Tc]Fucoidan SPECT/CT accurately diagnosed the autoimmune attack in the early steps of EAM and could be used to monitor disease evolution and therapy efficiency.</description><subject>Antibodies</subject><subject>Autoradiography</subject><subject>Brain</subject><subject>Brain natriuretic peptide</subject><subject>Calcium-binding protein</subject><subject>CD11b antigen</subject><subject>CD45 antigen</subject><subject>CD8 antigen</subject><subject>Computation</subject><subject>Computed tomography</subject><subject>Effusion</subject><subject>Flow cytometry</subject><subject>Fucoidan</subject><subject>Heart diseases</subject><subject>Histology</subject><subject>Imaging</subject><subject>Immunity</subject><subject>Immunization</subject><subject>Medicine</subject><subject>Medicine & Public Health</subject><subject>Myocarditis</subject><subject>Myosin</subject><subject>Necrosis</subject><subject>Neuroimaging</subject><subject>Photon emission</subject><subject>Radiology</subject><subject>Research Article</subject><subject>Selectins</subject><subject>Single photon emission computed tomography</subject><subject>Technetium</subject><subject>Technetium isotopes</subject><subject>Tomography</subject><subject>Troponin</subject><subject>Troponin T</subject><issn>1536-1632</issn><issn>1860-2002</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2020</creationdate><recordtype>article</recordtype><recordid>eNp9kUuLFDEURoMozkP_gAsJuHFTmlflsRzannGgRcGelUhI5TFmqErapApsf71pe1RwMYuQwD33u5ccAF5g9AYjJN5WjCkSHcKqHUZQJx-BUyw56ghC5HF795R3mFNyAs5qvUMIC0zoU3BCMaOESnUKvq1NGffwnZ-9nWNOMAe4ydaM8ad38HqalhTnPYwJrn_sfImTT7MZ4cUy53goevhh3_Di4hwrvKkx3cIvSk1b-_VysTk6k-DnT-vV9hl4EsxY_fP7-xzcXK63q_fd5uPV9epi01mG6NxRywUNntIwOEN7YZhQwhGHuVFUMSGx89zJYNEQOFc0sH6QzPbYmhDEwOg5eH3M3ZX8ffF11lOs1o-jST4vVRMiCVNMKtzQV_-hd3kpqW2nCUNc8V4y8iBFxGEHQkWjyJGyJddafNC79lmm7DVG-mBLH23pZkv_tqVla3p5H70Mk3d_W_7oaQA9ArWV0q0v_2Y_EPsLT02egA</recordid><startdate>20200601</startdate><enddate>20200601</enddate><creator>Vigne, Jonathan</creator><creator>Cognet, Thomas</creator><creator>Guedj, Kevin</creator><creator>Morvan, Marion</creator><creator>Merceron, Olivier</creator><creator>Louedec, Liliane</creator><creator>Choqueux, Christine</creator><creator>Nicoletti, Antonino</creator><creator>Escoubet, Brigitte</creator><creator>Chaubet, Frederic</creator><creator>Michel, Jean-Baptiste</creator><creator>Rouzet, François</creator><general>Springer International Publishing</general><general>Springer Nature B.V</general><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QO</scope><scope>8FD</scope><scope>FR3</scope><scope>K9.</scope><scope>NAPCQ</scope><scope>P64</scope><scope>7X8</scope><orcidid>https://orcid.org/0000-0002-6575-9224</orcidid></search><sort><creationdate>20200601</creationdate><title>Early Detection of Localized Immunity in Experimental Autoimmune Myocarditis Using [99mTc]Fucoidan SPECT</title><author>Vigne, Jonathan ; Cognet, Thomas ; Guedj, Kevin ; Morvan, Marion ; Merceron, Olivier ; Louedec, Liliane ; Choqueux, Christine ; Nicoletti, Antonino ; Escoubet, Brigitte ; Chaubet, Frederic ; Michel, Jean-Baptiste ; Rouzet, François</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c403t-3c673fe33fbda357a4797d2d16a9394781de6d8fc0bf6693f45b84c51caff7b43</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2020</creationdate><topic>Antibodies</topic><topic>Autoradiography</topic><topic>Brain</topic><topic>Brain natriuretic peptide</topic><topic>Calcium-binding protein</topic><topic>CD11b antigen</topic><topic>CD45 antigen</topic><topic>CD8 antigen</topic><topic>Computation</topic><topic>Computed tomography</topic><topic>Effusion</topic><topic>Flow cytometry</topic><topic>Fucoidan</topic><topic>Heart diseases</topic><topic>Histology</topic><topic>Imaging</topic><topic>Immunity</topic><topic>Immunization</topic><topic>Medicine</topic><topic>Medicine & Public Health</topic><topic>Myocarditis</topic><topic>Myosin</topic><topic>Necrosis</topic><topic>Neuroimaging</topic><topic>Photon emission</topic><topic>Radiology</topic><topic>Research Article</topic><topic>Selectins</topic><topic>Single photon emission computed tomography</topic><topic>Technetium</topic><topic>Technetium isotopes</topic><topic>Tomography</topic><topic>Troponin</topic><topic>Troponin T</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Vigne, Jonathan</creatorcontrib><creatorcontrib>Cognet, Thomas</creatorcontrib><creatorcontrib>Guedj, Kevin</creatorcontrib><creatorcontrib>Morvan, Marion</creatorcontrib><creatorcontrib>Merceron, Olivier</creatorcontrib><creatorcontrib>Louedec, Liliane</creatorcontrib><creatorcontrib>Choqueux, Christine</creatorcontrib><creatorcontrib>Nicoletti, Antonino</creatorcontrib><creatorcontrib>Escoubet, Brigitte</creatorcontrib><creatorcontrib>Chaubet, Frederic</creatorcontrib><creatorcontrib>Michel, Jean-Baptiste</creatorcontrib><creatorcontrib>Rouzet, François</creatorcontrib><collection>PubMed</collection><collection>CrossRef</collection><collection>Biotechnology Research Abstracts</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Nursing & Allied Health Premium</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Molecular imaging and biology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Vigne, Jonathan</au><au>Cognet, Thomas</au><au>Guedj, Kevin</au><au>Morvan, Marion</au><au>Merceron, Olivier</au><au>Louedec, Liliane</au><au>Choqueux, Christine</au><au>Nicoletti, Antonino</au><au>Escoubet, Brigitte</au><au>Chaubet, Frederic</au><au>Michel, Jean-Baptiste</au><au>Rouzet, François</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Early Detection of Localized Immunity in Experimental Autoimmune Myocarditis Using [99mTc]Fucoidan SPECT</atitle><jtitle>Molecular imaging and biology</jtitle><stitle>Mol Imaging Biol</stitle><addtitle>Mol Imaging Biol</addtitle><date>2020-06-01</date><risdate>2020</risdate><volume>22</volume><issue>3</issue><spage>643</spage><epage>652</epage><pages>643-652</pages><issn>1536-1632</issn><eissn>1860-2002</eissn><abstract>Purpose
The aim of the study was to evaluate the ability of technetium-99m-fucoidan ([
99m
Tc]fucoidan), a molecular imaging agent specific for selectins, in the assessment of early localized immunity in a rat model of experimental autoimmune myocarditis (EAM).
Procedures
EAM was induced in Lewis rats and troponin T; brain natriuretic peptide (BNP) and anti-myosin antibodies were measured in plasma. Separately, [
99m
Tc]fucoidan single-photon emission computed tomography (SPECT)/x-ray computed tomography (CT) was performed in the very early phase of myocarditis at 10, 15, and 21 days after immunization. Then, hearts were collected and used for autoradiography, well counting, histology, and flow cytometry analysis.
Results
The EAM acute phase is characterized by extensive myocardial necrosis, release of troponin and BNP, and pericardial effusion. [
99m
Tc]Fucoidan uptake was significantly increased in EAM compared with controls starting from D15. There was a close relationship between uptake of the tracer and myocardial content in CD45+, CD8+, CD11b+, and CD31+ cells.
Conclusions
[
99m
Tc]Fucoidan SPECT/CT accurately diagnosed the autoimmune attack in the early steps of EAM and could be used to monitor disease evolution and therapy efficiency.</abstract><cop>Cham</cop><pub>Springer International Publishing</pub><pmid>31432389</pmid><doi>10.1007/s11307-019-01420-8</doi><tpages>10</tpages><orcidid>https://orcid.org/0000-0002-6575-9224</orcidid></addata></record> |
| fulltext | fulltext |
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| ispartof | Molecular imaging and biology, 2020-06, Vol.22 (3), p.643-652 |
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| source | SpringerLink Journals |
| subjects | Antibodies Autoradiography Brain Brain natriuretic peptide Calcium-binding protein CD11b antigen CD45 antigen CD8 antigen Computation Computed tomography Effusion Flow cytometry Fucoidan Heart diseases Histology Imaging Immunity Immunization Medicine Medicine & Public Health Myocarditis Myosin Necrosis Neuroimaging Photon emission Radiology Research Article Selectins Single photon emission computed tomography Technetium Technetium isotopes Tomography Troponin Troponin T |
| title | Early Detection of Localized Immunity in Experimental Autoimmune Myocarditis Using [99mTc]Fucoidan SPECT |
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