A phenotypic screening bioassay for Escherichia coli stress and antibiotic responses based on Fourier‐transform infrared (FTIR) spectroscopy and multivariate analysis

Aims To develop and optimize a Fourier‐transform infrared spectroscopy (FTIRS) phenotypic screening bioassay for stress responses, regarding the effect of nutrient content, bacterial growth phase and stress agent exposure time. Methods and Results A high‐throughput FTIRS bioassay was developed to di...

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Veröffentlicht in:	Journal of applied microbiology 2019-12, Vol.127 (6), p.1776-1789
Hauptverfasser:	Ribeiro da Cunha, B., Fonseca, L.P., Calado, C.R.C.
Format:	Artikel
Sprache:	eng
Schlagworte:	Anti-Bacterial Agents - pharmacology Antibiotics Bacteria Bacteriological Techniques - methods Bioassays Cellular stress response Cluster analysis Clustering E coli Empirical analysis Escherichia coli Escherichia coli - drug effects Ethanol Exposure Fourier‐transform infrared spectroscopy High-Throughput Screening Assays Hydrochloric acid Infrared analysis Infrared spectroscopy Metabolism Multivariate Analysis Nutrient content Nutrients Optimization Parameters Phenotype Principal components analysis Reproducibility Reproducibility of Results Screening Sodium chloride Sodium hydroxide Sodium hypochlorite Spectra Spectroscopy, Fourier Transform Infrared Spectrum analysis stress response Stress, Physiological - drug effects
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