The role of silk in courtship and communication in mygalomorph spiders: Do males regulate their courtship in response to female mating status?

•Females do not use airborne or cuticular pheromones to trigger male courtship.•A.centralis females use silk-borne signals to trigger male courtship•Different female body conditions have no effect on male mate choice.•Males invest higher courtship effort on silk from unmated than mated females. In spiders, pheromones are known to be responsible for attracting the opposite sex, eliciting male searching and courtship behaviors, as well as for synchronizing potential mates in space and time. Most spiders are cannibalistic and aggressive. Thus, early recognition of a female as a possible mate is essential for males, who may suffer high energetic or reproductive costs to the extreme of losing all fitness opportunities. In Acanthogonatus centralis Goloboff 1995, a mygalomorph spider, what female signs might be triggering male courtship behavior remain unknown, as well as whether males can discriminate between females. The aims of the present work were (1) establishing whether males can detect the presence of females using airborne and silk-borne signals and (2) determining whether males can discriminate the reproductive status and body condition of females. We found no evidence that airborne pheromones play a role in the sexual communication of A. centralis, but silk-bound contact signals function as a female advertisement. Also, this is the first study that demonstrates that male mygalomorph spiders can discriminate between different signals on silk through direct contact, showing a preference for unmated females.