Changes in the oxidative stress factors and inflammatory proteins following the treatment of BPH‐induced dogs with an anti‐proliferative agent called tadalafil

Background Finding a medical treatment which can combat cell proliferation and relax smooth muscles in canine benign prostatic hyperplasia (BPH) appears to be imperative. Aims This study aimed to evaluate the oxidative stress and inflammatory proteins following the treatment of dogs induced for BPH with an anti‐proliferative agent called tadalafil. Materials and Methods Twenty‐five adult intact male dogs were randomly designated into five groups (n = 5): Control group was not induced for BPH and not treated with tadalafil; dogs induced for BPH by testosterone enanthate and estradiol benzoate and treated with tadalafil (5 mg/day P.O.); dogs which received tadalafil (5 mg/day P.O.); dogs induced for BPH and treated with castration; and dogs induced for BPH. Oxidative stress factors (glutathione peroxidase [GPX], superoxide dismutase [SOD], catalase) and inflammatory proteins (haptoglobin, serum amyloid A [SAA], malondialdehyde [MDA]) were measured in the blood serum for four sequential weeks. Results Glutathione peroxidase and SOD serum levels declined in dogs in the BPH‐induced group compared to those in the control group. Those levels diminished in BPH‐induced castrated and tadalafil‐treated groups. The changes in the GPX and SOD serum concentrations were not significant between the BPH‐induced castrated group and BPH‐induced tadalafil‐treated group. Moreover, MDA concentration increased slightly in groups with BPH and groups which were castrated. Generally, however, there were no significant differences in the MDA serum concentrations between other groups. Haptoglobin and SAA concentrations increased in BPH‐castrated group. Also, the differences in haptoglobin and SAA were not significant between the groups. Conclusion Tadalafil could not control oxidative stress and inflammatory mediators which happened during BPH in dogs.