Structural and functional characterisation of human RNA helicase DHX8 provides insights into the mechanism of RNA-stimulated ADP release

DHX8 is a crucial DEAH-box RNA helicase involved in splicing and required for the release of mature mRNA from the spliceosome. Here, we report the biochemical characterisation of full-length human DHX8 and the catalytically active helicase core DHX8Δ547, alongside crystal structures of DHX8Δ547 boun...

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Veröffentlicht in:	Biochemical journal 2019-09, Vol.476 (18), p.2521-2543
Hauptverfasser:	Felisberto-Rodrigues, Catarina, Thomas, Jemima C, McAndrew, Craig, Le Bihan, Yann-Vaï, Burke, Rosemary, Workman, Paul, van Montfort, Rob L M
Format:	Artikel
Sprache:	eng
Schlagworte:	Adenosine Diphosphate - chemistry Adenosine Diphosphate - genetics Adenosine Diphosphate - metabolism Amino Acid Motifs DEAD-box RNA Helicases - chemistry DEAD-box RNA Helicases - genetics DEAD-box RNA Helicases - metabolism Humans Poly A - chemistry Poly A - genetics Poly A - metabolism Protein Binding RNA - chemistry RNA - genetics RNA - metabolism RNA Splicing Factors - chemistry RNA Splicing Factors - genetics RNA Splicing Factors - metabolism Structure-Activity Relationship
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