Identification of differential abundances of mRNA transcript in cumulus cells and CCND1 associated with yak oocyte developmental competence
The development of an accurate and noninvasive preselection process for competent oocytes is essential to achieve a highly efficient in vitro production (IVP) of embryos. Cumulus cells (CCs) have important functions in oocyte growth, development, maturation, and fertilization. It, therefore, is impo...
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| Veröffentlicht in: | Animal reproduction science 2019-09, Vol.208, p.106135-106135, Article 106135 |
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| creator | Xiong, X.R. Lan, D.L. Li, J. Yin, S. Xiong, Y. Zi, X.D. |
| description | The development of an accurate and noninvasive preselection process for competent oocytes is essential to achieve a highly efficient in vitro production (IVP) of embryos. Cumulus cells (CCs) have important functions in oocyte growth, development, maturation, and fertilization. It, therefore, is important to know if the quality of oocytes can be ascertained by assessment of gene expression of the surrounding CCs or not. The aim of this study was to identify differentially expressed genes in yak CCs from oocytes with varying developmental competences as possible biomarkers for distinguishing oocyte competence. The isolated CCs were pooled into immature and mature groups in accordance with the maturation outcome of oocytes. A total of 9516 genes were differentially expressed in the two CC categories (P |
| doi_str_mv | 10.1016/j.anireprosci.2019.106135 |
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Cumulus cells (CCs) have important functions in oocyte growth, development, maturation, and fertilization. It, therefore, is important to know if the quality of oocytes can be ascertained by assessment of gene expression of the surrounding CCs or not. The aim of this study was to identify differentially expressed genes in yak CCs from oocytes with varying developmental competences as possible biomarkers for distinguishing oocyte competence. The isolated CCs were pooled into immature and mature groups in accordance with the maturation outcome of oocytes. A total of 9516 genes were differentially expressed in the two CC categories (P < 0.05). With a minimum change of 2.5-fold, 45 up-regulated and 79 down-regulated genes were observed in CCs belonging to the mature group compared with those in the immature group (P < 0.01). These genes were primarily enriched for the cell cycle, meiosis, cell signaling, metabolism, and apoptosis. The selected candidate genes (CCND1, BMP15, GDF9, H19, KLF4, GPC1, SYCP3, and CTSB) were validated using quantitative real-time polymerase chain reaction (RT-qPCR) and there were expression patterns similar to those detected with transcriptome analysis. The CCs from fertilized oocytes arrested at the 2-cell (2-cell group), or 8-cell (8-cell group) stages or that developed into blastocysts (the blastocyst group) had a 1.5-, 1.8-, and 2.3-fold increase, respectively, in mRNA relative abundance of CCND1 compared with CCs from unfertilized oocytes (P < 0.05). The results with the RT-qPCR analysis confirmed that the relative abundance of CCND1 mRNA in CCs was associated with oocyte developmental competence. In conclusion, RNA-Seq is useful in extracting transcriptomes and selecting markers associated with oocyte developmental competence. Furthermore, the expression of the CCND1 gene in yak CCs can be used to preselect oocytes for IVP efficiency.</description><identifier>ISSN: 0378-4320</identifier><identifier>EISSN: 1873-2232</identifier><identifier>DOI: 10.1016/j.anireprosci.2019.106135</identifier><identifier>PMID: 31405458</identifier><language>eng</language><publisher>Netherlands: Elsevier B.V</publisher><subject>Animals ; Base Sequence ; Cattle ; CCND1 ; Cumulus cells ; Cumulus Cells - physiology ; Cyclin D1 - genetics ; Cyclin D1 - metabolism ; Embryo Culture Techniques - veterinary ; Expression ; Female ; Fertilization in Vitro - veterinary ; In Vitro Oocyte Maturation Techniques - veterinary ; Oocyte quality ; Oocytes - physiology ; Reproducibility of Results ; RNA, Messenger - metabolism ; Transcriptome ; Yak</subject><ispartof>Animal reproduction science, 2019-09, Vol.208, p.106135-106135, Article 106135</ispartof><rights>2019 Elsevier B.V.</rights><rights>Copyright © 2019 Elsevier B.V. All rights reserved.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c377t-118eb73cdd5d0fb3751ee7ec3f4df27a42aad3a2bdba48036df0208c7712e4ab3</citedby><cites>FETCH-LOGICAL-c377t-118eb73cdd5d0fb3751ee7ec3f4df27a42aad3a2bdba48036df0208c7712e4ab3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://www.sciencedirect.com/science/article/pii/S0378432019303549$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,776,780,3537,27901,27902,65306</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/31405458$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Xiong, X.R.</creatorcontrib><creatorcontrib>Lan, D.L.</creatorcontrib><creatorcontrib>Li, J.</creatorcontrib><creatorcontrib>Yin, S.</creatorcontrib><creatorcontrib>Xiong, Y.</creatorcontrib><creatorcontrib>Zi, X.D.</creatorcontrib><title>Identification of differential abundances of mRNA transcript in cumulus cells and CCND1 associated with yak oocyte developmental competence</title><title>Animal reproduction science</title><addtitle>Anim Reprod Sci</addtitle><description>The development of an accurate and noninvasive preselection process for competent oocytes is essential to achieve a highly efficient in vitro production (IVP) of embryos. Cumulus cells (CCs) have important functions in oocyte growth, development, maturation, and fertilization. It, therefore, is important to know if the quality of oocytes can be ascertained by assessment of gene expression of the surrounding CCs or not. The aim of this study was to identify differentially expressed genes in yak CCs from oocytes with varying developmental competences as possible biomarkers for distinguishing oocyte competence. The isolated CCs were pooled into immature and mature groups in accordance with the maturation outcome of oocytes. A total of 9516 genes were differentially expressed in the two CC categories (P < 0.05). With a minimum change of 2.5-fold, 45 up-regulated and 79 down-regulated genes were observed in CCs belonging to the mature group compared with those in the immature group (P < 0.01). These genes were primarily enriched for the cell cycle, meiosis, cell signaling, metabolism, and apoptosis. The selected candidate genes (CCND1, BMP15, GDF9, H19, KLF4, GPC1, SYCP3, and CTSB) were validated using quantitative real-time polymerase chain reaction (RT-qPCR) and there were expression patterns similar to those detected with transcriptome analysis. The CCs from fertilized oocytes arrested at the 2-cell (2-cell group), or 8-cell (8-cell group) stages or that developed into blastocysts (the blastocyst group) had a 1.5-, 1.8-, and 2.3-fold increase, respectively, in mRNA relative abundance of CCND1 compared with CCs from unfertilized oocytes (P < 0.05). The results with the RT-qPCR analysis confirmed that the relative abundance of CCND1 mRNA in CCs was associated with oocyte developmental competence. In conclusion, RNA-Seq is useful in extracting transcriptomes and selecting markers associated with oocyte developmental competence. Furthermore, the expression of the CCND1 gene in yak CCs can be used to preselect oocytes for IVP efficiency.</description><subject>Animals</subject><subject>Base Sequence</subject><subject>Cattle</subject><subject>CCND1</subject><subject>Cumulus cells</subject><subject>Cumulus Cells - physiology</subject><subject>Cyclin D1 - genetics</subject><subject>Cyclin D1 - metabolism</subject><subject>Embryo Culture Techniques - veterinary</subject><subject>Expression</subject><subject>Female</subject><subject>Fertilization in Vitro - veterinary</subject><subject>In Vitro Oocyte Maturation Techniques - veterinary</subject><subject>Oocyte quality</subject><subject>Oocytes - physiology</subject><subject>Reproducibility of Results</subject><subject>RNA, Messenger - metabolism</subject><subject>Transcriptome</subject><subject>Yak</subject><issn>0378-4320</issn><issn>1873-2232</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2019</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqNUcmO1DAQtRCIaRp-AZkblzRekjh9HIVtpNEgIThbFbss3CRxsJ1B_Q38NI56QBw5lVT1Fvs9Ql5xduCMt29OB5h9xCWGZPxBMH4s-5bL5hHZ8U7JSggpHpMdk6qrainYFXmW0okxptr2-JRcSV6zpm66Hfl1Y3HO3nkD2YeZBketdw7jtoWRwrDOFmaDaTtNn--uaY4wJxP9kqmfqVmndVwTNTiOicJsad_fveUUUgrGQ0ZLf_r8jZ7hOw3BnDNSi_c4hmUqFsXBhGnBjMXiOXniYEz44mHuydf37770H6vbTx9u-uvbykilcsV5h4OSxtrGMjdI1XBEhUa62jqhoBYAVoIY7AB1x2RrHROsM0pxgTUMck9eX3RLgD9WTFlPPm3vhxnDmrQQSijZscLdk-MFakrWKaLTS_QTxLPmTG9d6JP-pwu9daEvXRTuywebdZjQ_mX-Cb8A-gsAy2fvPUZdJLYgbBE0Wdvg_8PmNxBtpGM</recordid><startdate>201909</startdate><enddate>201909</enddate><creator>Xiong, X.R.</creator><creator>Lan, D.L.</creator><creator>Li, J.</creator><creator>Yin, S.</creator><creator>Xiong, Y.</creator><creator>Zi, X.D.</creator><general>Elsevier B.V</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>201909</creationdate><title>Identification of differential abundances of mRNA transcript in cumulus cells and CCND1 associated with yak oocyte developmental competence</title><author>Xiong, X.R. ; Lan, D.L. ; Li, J. ; Yin, S. ; Xiong, Y. ; Zi, X.D.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c377t-118eb73cdd5d0fb3751ee7ec3f4df27a42aad3a2bdba48036df0208c7712e4ab3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2019</creationdate><topic>Animals</topic><topic>Base Sequence</topic><topic>Cattle</topic><topic>CCND1</topic><topic>Cumulus cells</topic><topic>Cumulus Cells - physiology</topic><topic>Cyclin D1 - genetics</topic><topic>Cyclin D1 - metabolism</topic><topic>Embryo Culture Techniques - veterinary</topic><topic>Expression</topic><topic>Female</topic><topic>Fertilization in Vitro - veterinary</topic><topic>In Vitro Oocyte Maturation Techniques - veterinary</topic><topic>Oocyte quality</topic><topic>Oocytes - physiology</topic><topic>Reproducibility of Results</topic><topic>RNA, Messenger - metabolism</topic><topic>Transcriptome</topic><topic>Yak</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Xiong, X.R.</creatorcontrib><creatorcontrib>Lan, D.L.</creatorcontrib><creatorcontrib>Li, J.</creatorcontrib><creatorcontrib>Yin, S.</creatorcontrib><creatorcontrib>Xiong, Y.</creatorcontrib><creatorcontrib>Zi, X.D.</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Animal reproduction science</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Xiong, X.R.</au><au>Lan, D.L.</au><au>Li, J.</au><au>Yin, S.</au><au>Xiong, Y.</au><au>Zi, X.D.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Identification of differential abundances of mRNA transcript in cumulus cells and CCND1 associated with yak oocyte developmental competence</atitle><jtitle>Animal reproduction science</jtitle><addtitle>Anim Reprod Sci</addtitle><date>2019-09</date><risdate>2019</risdate><volume>208</volume><spage>106135</spage><epage>106135</epage><pages>106135-106135</pages><artnum>106135</artnum><issn>0378-4320</issn><eissn>1873-2232</eissn><abstract>The development of an accurate and noninvasive preselection process for competent oocytes is essential to achieve a highly efficient in vitro production (IVP) of embryos. Cumulus cells (CCs) have important functions in oocyte growth, development, maturation, and fertilization. It, therefore, is important to know if the quality of oocytes can be ascertained by assessment of gene expression of the surrounding CCs or not. The aim of this study was to identify differentially expressed genes in yak CCs from oocytes with varying developmental competences as possible biomarkers for distinguishing oocyte competence. The isolated CCs were pooled into immature and mature groups in accordance with the maturation outcome of oocytes. A total of 9516 genes were differentially expressed in the two CC categories (P < 0.05). With a minimum change of 2.5-fold, 45 up-regulated and 79 down-regulated genes were observed in CCs belonging to the mature group compared with those in the immature group (P < 0.01). These genes were primarily enriched for the cell cycle, meiosis, cell signaling, metabolism, and apoptosis. The selected candidate genes (CCND1, BMP15, GDF9, H19, KLF4, GPC1, SYCP3, and CTSB) were validated using quantitative real-time polymerase chain reaction (RT-qPCR) and there were expression patterns similar to those detected with transcriptome analysis. The CCs from fertilized oocytes arrested at the 2-cell (2-cell group), or 8-cell (8-cell group) stages or that developed into blastocysts (the blastocyst group) had a 1.5-, 1.8-, and 2.3-fold increase, respectively, in mRNA relative abundance of CCND1 compared with CCs from unfertilized oocytes (P < 0.05). The results with the RT-qPCR analysis confirmed that the relative abundance of CCND1 mRNA in CCs was associated with oocyte developmental competence. In conclusion, RNA-Seq is useful in extracting transcriptomes and selecting markers associated with oocyte developmental competence. Furthermore, the expression of the CCND1 gene in yak CCs can be used to preselect oocytes for IVP efficiency.</abstract><cop>Netherlands</cop><pub>Elsevier B.V</pub><pmid>31405458</pmid><doi>10.1016/j.anireprosci.2019.106135</doi><tpages>1</tpages></addata></record> |
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| subjects | Animals Base Sequence Cattle CCND1 Cumulus cells Cumulus Cells - physiology Cyclin D1 - genetics Cyclin D1 - metabolism Embryo Culture Techniques - veterinary Expression Female Fertilization in Vitro - veterinary In Vitro Oocyte Maturation Techniques - veterinary Oocyte quality Oocytes - physiology Reproducibility of Results RNA, Messenger - metabolism Transcriptome Yak |
| title | Identification of differential abundances of mRNA transcript in cumulus cells and CCND1 associated with yak oocyte developmental competence |
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