Assessment of angiogenesis in rabbit orthotropic liver tumors using three-dimensional dynamic contrast-enhanced ultrasound compared with two-dimensional DCE-US
Objectives To evaluate quantitative three-dimensional (3D) dynamic contrast-enhanced ultrasound (DCE-US) in the assessment of tumor angiogenesis using an orthotropic liver tumor model. Methods Nine New Zealand white rabbits with liver orthotropic VX2 tumors were established and imaged by two-dimensi...
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| Veröffentlicht in: | Japanese journal of radiology 2019-10, Vol.37 (10), p.701-709 |
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| container_title | Japanese journal of radiology |
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| creator | Zheng, Qiao Zhang, Jian-chao Wang, Zhu Ruan, Si-Min Li, Wei Pan, Fu-Shun Chen, Li-Da Zhang, Yu-Chen Wu, Wen-Xin Xie, Xiao-Yan Lu, Ming-De Shan, Quan-Yuan Wang, Wei |
| description | Objectives
To evaluate quantitative three-dimensional (3D) dynamic contrast-enhanced ultrasound (DCE-US) in the assessment of tumor angiogenesis using an orthotropic liver tumor model.
Methods
Nine New Zealand white rabbits with liver orthotropic VX2 tumors were established and imaged by two-dimensional (2D) and 3D DCE-US after SonoVue
®
bolus injections. The intraclass correlation coefficients of perfusion parameters, including peak intensity (PI), mean transit time, time to peak, and area under the curve, were calculated based on time-intensity curve. The percentage area of microvascular (PAMV) and the expression of vascular endothelial growth factor (VEGF) were both evaluated by immunohistochemical analysis and weighted by the tumor activity area ratio. Correlations between quantitative and histologic parameters were analyzed.
Results
The reproducibility of 3D DCE-US quantitative parameters was excellent (ICC 0.91–0.99); but only PI showed high reproducibility (ICC 0.97) in 2D. None of the parameters of quantitative 2D DCE-US were significantly correlated with weighted PAMV or VEGF. For 3D DCE-US, there was a positive correlation between PI and weighted PAMV (
r
= 0.74,
P
= 0.04) as well as VEGF (
r
= 0.79,
P
= 0.02).
Conclusion
Quantitative parameters of 3D DCE-US show feasibility, higher reproducibility and accuracy for the assessment of tumor angiogenesis using an orthotropic liver tumor model compared with 2D DCE-US. |
| doi_str_mv | 10.1007/s11604-019-00861-z |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_2272222222</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>2270822779</sourcerecordid><originalsourceid>FETCH-LOGICAL-c375t-cab46234c06c083e289b9f0f30beb542691c98c3ee02933f2e5aca23812e88543</originalsourceid><addsrcrecordid>eNp9kc1u1DAUhS0EoqXwAiyQJTZsDP6bxFlWQ_mRKrGASuwsx7mZcZXYg6_Tqn0ZXhV3phTBAi_sq3u-e2z5EPJS8LeC8_YdCtFwzbjoGOemEez2ETkWpmmZ4Ob744e6FUfkGeIl541WWj8lR0poLlopj8nPU0RAnCEWmkbq4iakDUTAgDREml3fh6rksk0lp13wdApXkGlZ5pSRLhjihpZtBmBDqC4YUnQTHW6imyvsUyzZYWEQty56GOgy3TXSEocqzjuXa-86lC0t1-kvi_frM3bx9Tl5MroJ4cX9eUIuPpx9W39i518-fl6fnjOv2lVh3vW6kUp73nhuFEjT9d3IR8V76FdaNp3wnfEKgMtOqVHCynknlRESjFlpdULeHHx3Of1YAIudA3qYJhchLWilrN-1XxV9_Q96mZZcn7ynuKlb21VKHiifE2KG0e5ymF2-sYLbu_jsIT5b47P7-OxtHXp1b730MwwPI7_zqoA6AFiluIH85-7_2P4ClgepdA</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>2270822779</pqid></control><display><type>article</type><title>Assessment of angiogenesis in rabbit orthotropic liver tumors using three-dimensional dynamic contrast-enhanced ultrasound compared with two-dimensional DCE-US</title><source>MEDLINE</source><source>Springer Nature - Complete Springer Journals</source><creator>Zheng, Qiao ; Zhang, Jian-chao ; Wang, Zhu ; Ruan, Si-Min ; Li, Wei ; Pan, Fu-Shun ; Chen, Li-Da ; Zhang, Yu-Chen ; Wu, Wen-Xin ; Xie, Xiao-Yan ; Lu, Ming-De ; Shan, Quan-Yuan ; Wang, Wei</creator><creatorcontrib>Zheng, Qiao ; Zhang, Jian-chao ; Wang, Zhu ; Ruan, Si-Min ; Li, Wei ; Pan, Fu-Shun ; Chen, Li-Da ; Zhang, Yu-Chen ; Wu, Wen-Xin ; Xie, Xiao-Yan ; Lu, Ming-De ; Shan, Quan-Yuan ; Wang, Wei</creatorcontrib><description>Objectives
To evaluate quantitative three-dimensional (3D) dynamic contrast-enhanced ultrasound (DCE-US) in the assessment of tumor angiogenesis using an orthotropic liver tumor model.
Methods
Nine New Zealand white rabbits with liver orthotropic VX2 tumors were established and imaged by two-dimensional (2D) and 3D DCE-US after SonoVue
®
bolus injections. The intraclass correlation coefficients of perfusion parameters, including peak intensity (PI), mean transit time, time to peak, and area under the curve, were calculated based on time-intensity curve. The percentage area of microvascular (PAMV) and the expression of vascular endothelial growth factor (VEGF) were both evaluated by immunohistochemical analysis and weighted by the tumor activity area ratio. Correlations between quantitative and histologic parameters were analyzed.
Results
The reproducibility of 3D DCE-US quantitative parameters was excellent (ICC 0.91–0.99); but only PI showed high reproducibility (ICC 0.97) in 2D. None of the parameters of quantitative 2D DCE-US were significantly correlated with weighted PAMV or VEGF. For 3D DCE-US, there was a positive correlation between PI and weighted PAMV (
r
= 0.74,
P
= 0.04) as well as VEGF (
r
= 0.79,
P
= 0.02).
Conclusion
Quantitative parameters of 3D DCE-US show feasibility, higher reproducibility and accuracy for the assessment of tumor angiogenesis using an orthotropic liver tumor model compared with 2D DCE-US.</description><identifier>ISSN: 1867-1071</identifier><identifier>EISSN: 1867-108X</identifier><identifier>DOI: 10.1007/s11604-019-00861-z</identifier><identifier>PMID: 31401722</identifier><language>eng</language><publisher>Tokyo: Springer Japan</publisher><subject>Angiogenesis ; Animals ; Contrast Media ; Correlation coefficients ; Disease Models, Animal ; Drug delivery systems ; Feasibility studies ; Growth factors ; Humans ; Image Enhancement - methods ; Imaging ; Imaging, Three-Dimensional - methods ; Liver ; Liver - diagnostic imaging ; Liver - pathology ; Liver cancer ; Liver Neoplasms - diagnostic imaging ; Liver Neoplasms - pathology ; Mathematical models ; Medicine ; Medicine & Public Health ; Microvasculature ; Neovascularization, Pathologic - diagnostic imaging ; Neovascularization, Pathologic - pathology ; Nuclear Medicine ; Original Article ; Parameters ; Perfusion ; Rabbits ; Radiology ; Radiotherapy ; Reproducibility ; Reproducibility of Results ; Transit time ; Tumors ; Two dimensional models ; Ultrasonic imaging ; Ultrasonography - methods ; Ultrasound ; Vascular endothelial growth factor</subject><ispartof>Japanese journal of radiology, 2019-10, Vol.37 (10), p.701-709</ispartof><rights>Japan Radiological Society 2019</rights><rights>Japanese Journal of Radiology is a copyright of Springer, (2019). All Rights Reserved.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c375t-cab46234c06c083e289b9f0f30beb542691c98c3ee02933f2e5aca23812e88543</citedby><cites>FETCH-LOGICAL-c375t-cab46234c06c083e289b9f0f30beb542691c98c3ee02933f2e5aca23812e88543</cites><orcidid>0000-0002-9485-583X</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://link.springer.com/content/pdf/10.1007/s11604-019-00861-z$$EPDF$$P50$$Gspringer$$H</linktopdf><linktohtml>$$Uhttps://link.springer.com/10.1007/s11604-019-00861-z$$EHTML$$P50$$Gspringer$$H</linktohtml><link.rule.ids>314,776,780,27901,27902,41464,42533,51294</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/31401722$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Zheng, Qiao</creatorcontrib><creatorcontrib>Zhang, Jian-chao</creatorcontrib><creatorcontrib>Wang, Zhu</creatorcontrib><creatorcontrib>Ruan, Si-Min</creatorcontrib><creatorcontrib>Li, Wei</creatorcontrib><creatorcontrib>Pan, Fu-Shun</creatorcontrib><creatorcontrib>Chen, Li-Da</creatorcontrib><creatorcontrib>Zhang, Yu-Chen</creatorcontrib><creatorcontrib>Wu, Wen-Xin</creatorcontrib><creatorcontrib>Xie, Xiao-Yan</creatorcontrib><creatorcontrib>Lu, Ming-De</creatorcontrib><creatorcontrib>Shan, Quan-Yuan</creatorcontrib><creatorcontrib>Wang, Wei</creatorcontrib><title>Assessment of angiogenesis in rabbit orthotropic liver tumors using three-dimensional dynamic contrast-enhanced ultrasound compared with two-dimensional DCE-US</title><title>Japanese journal of radiology</title><addtitle>Jpn J Radiol</addtitle><addtitle>Jpn J Radiol</addtitle><description>Objectives
To evaluate quantitative three-dimensional (3D) dynamic contrast-enhanced ultrasound (DCE-US) in the assessment of tumor angiogenesis using an orthotropic liver tumor model.
Methods
Nine New Zealand white rabbits with liver orthotropic VX2 tumors were established and imaged by two-dimensional (2D) and 3D DCE-US after SonoVue
®
bolus injections. The intraclass correlation coefficients of perfusion parameters, including peak intensity (PI), mean transit time, time to peak, and area under the curve, were calculated based on time-intensity curve. The percentage area of microvascular (PAMV) and the expression of vascular endothelial growth factor (VEGF) were both evaluated by immunohistochemical analysis and weighted by the tumor activity area ratio. Correlations between quantitative and histologic parameters were analyzed.
Results
The reproducibility of 3D DCE-US quantitative parameters was excellent (ICC 0.91–0.99); but only PI showed high reproducibility (ICC 0.97) in 2D. None of the parameters of quantitative 2D DCE-US were significantly correlated with weighted PAMV or VEGF. For 3D DCE-US, there was a positive correlation between PI and weighted PAMV (
r
= 0.74,
P
= 0.04) as well as VEGF (
r
= 0.79,
P
= 0.02).
Conclusion
Quantitative parameters of 3D DCE-US show feasibility, higher reproducibility and accuracy for the assessment of tumor angiogenesis using an orthotropic liver tumor model compared with 2D DCE-US.</description><subject>Angiogenesis</subject><subject>Animals</subject><subject>Contrast Media</subject><subject>Correlation coefficients</subject><subject>Disease Models, Animal</subject><subject>Drug delivery systems</subject><subject>Feasibility studies</subject><subject>Growth factors</subject><subject>Humans</subject><subject>Image Enhancement - methods</subject><subject>Imaging</subject><subject>Imaging, Three-Dimensional - methods</subject><subject>Liver</subject><subject>Liver - diagnostic imaging</subject><subject>Liver - pathology</subject><subject>Liver cancer</subject><subject>Liver Neoplasms - diagnostic imaging</subject><subject>Liver Neoplasms - pathology</subject><subject>Mathematical models</subject><subject>Medicine</subject><subject>Medicine & Public Health</subject><subject>Microvasculature</subject><subject>Neovascularization, Pathologic - diagnostic imaging</subject><subject>Neovascularization, Pathologic - pathology</subject><subject>Nuclear Medicine</subject><subject>Original Article</subject><subject>Parameters</subject><subject>Perfusion</subject><subject>Rabbits</subject><subject>Radiology</subject><subject>Radiotherapy</subject><subject>Reproducibility</subject><subject>Reproducibility of Results</subject><subject>Transit time</subject><subject>Tumors</subject><subject>Two dimensional models</subject><subject>Ultrasonic imaging</subject><subject>Ultrasonography - methods</subject><subject>Ultrasound</subject><subject>Vascular endothelial growth factor</subject><issn>1867-1071</issn><issn>1867-108X</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2019</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>BENPR</sourceid><recordid>eNp9kc1u1DAUhS0EoqXwAiyQJTZsDP6bxFlWQ_mRKrGASuwsx7mZcZXYg6_Tqn0ZXhV3phTBAi_sq3u-e2z5EPJS8LeC8_YdCtFwzbjoGOemEez2ETkWpmmZ4Ob744e6FUfkGeIl541WWj8lR0poLlopj8nPU0RAnCEWmkbq4iakDUTAgDREml3fh6rksk0lp13wdApXkGlZ5pSRLhjihpZtBmBDqC4YUnQTHW6imyvsUyzZYWEQty56GOgy3TXSEocqzjuXa-86lC0t1-kvi_frM3bx9Tl5MroJ4cX9eUIuPpx9W39i518-fl6fnjOv2lVh3vW6kUp73nhuFEjT9d3IR8V76FdaNp3wnfEKgMtOqVHCynknlRESjFlpdULeHHx3Of1YAIudA3qYJhchLWilrN-1XxV9_Q96mZZcn7ynuKlb21VKHiifE2KG0e5ymF2-sYLbu_jsIT5b47P7-OxtHXp1b730MwwPI7_zqoA6AFiluIH85-7_2P4ClgepdA</recordid><startdate>20191001</startdate><enddate>20191001</enddate><creator>Zheng, Qiao</creator><creator>Zhang, Jian-chao</creator><creator>Wang, Zhu</creator><creator>Ruan, Si-Min</creator><creator>Li, Wei</creator><creator>Pan, Fu-Shun</creator><creator>Chen, Li-Da</creator><creator>Zhang, Yu-Chen</creator><creator>Wu, Wen-Xin</creator><creator>Xie, Xiao-Yan</creator><creator>Lu, Ming-De</creator><creator>Shan, Quan-Yuan</creator><creator>Wang, Wei</creator><general>Springer Japan</general><general>Springer Nature B.V</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7QO</scope><scope>7RV</scope><scope>7TK</scope><scope>7U7</scope><scope>7X7</scope><scope>7XB</scope><scope>88E</scope><scope>8AO</scope><scope>8FD</scope><scope>8FE</scope><scope>8FG</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>ARAPS</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BGLVJ</scope><scope>BHPHI</scope><scope>C1K</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FR3</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>KB0</scope><scope>LK8</scope><scope>M0S</scope><scope>M1P</scope><scope>M7P</scope><scope>NAPCQ</scope><scope>P5Z</scope><scope>P62</scope><scope>P64</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>7X8</scope><orcidid>https://orcid.org/0000-0002-9485-583X</orcidid></search><sort><creationdate>20191001</creationdate><title>Assessment of angiogenesis in rabbit orthotropic liver tumors using three-dimensional dynamic contrast-enhanced ultrasound compared with two-dimensional DCE-US</title><author>Zheng, Qiao ; Zhang, Jian-chao ; Wang, Zhu ; Ruan, Si-Min ; Li, Wei ; Pan, Fu-Shun ; Chen, Li-Da ; Zhang, Yu-Chen ; Wu, Wen-Xin ; Xie, Xiao-Yan ; Lu, Ming-De ; Shan, Quan-Yuan ; Wang, Wei</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c375t-cab46234c06c083e289b9f0f30beb542691c98c3ee02933f2e5aca23812e88543</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2019</creationdate><topic>Angiogenesis</topic><topic>Animals</topic><topic>Contrast Media</topic><topic>Correlation coefficients</topic><topic>Disease Models, Animal</topic><topic>Drug delivery systems</topic><topic>Feasibility studies</topic><topic>Growth factors</topic><topic>Humans</topic><topic>Image Enhancement - methods</topic><topic>Imaging</topic><topic>Imaging, Three-Dimensional - methods</topic><topic>Liver</topic><topic>Liver - diagnostic imaging</topic><topic>Liver - pathology</topic><topic>Liver cancer</topic><topic>Liver Neoplasms - diagnostic imaging</topic><topic>Liver Neoplasms - pathology</topic><topic>Mathematical models</topic><topic>Medicine</topic><topic>Medicine & Public Health</topic><topic>Microvasculature</topic><topic>Neovascularization, Pathologic - diagnostic imaging</topic><topic>Neovascularization, Pathologic - pathology</topic><topic>Nuclear Medicine</topic><topic>Original Article</topic><topic>Parameters</topic><topic>Perfusion</topic><topic>Rabbits</topic><topic>Radiology</topic><topic>Radiotherapy</topic><topic>Reproducibility</topic><topic>Reproducibility of Results</topic><topic>Transit time</topic><topic>Tumors</topic><topic>Two dimensional models</topic><topic>Ultrasonic imaging</topic><topic>Ultrasonography - methods</topic><topic>Ultrasound</topic><topic>Vascular endothelial growth factor</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Zheng, Qiao</creatorcontrib><creatorcontrib>Zhang, Jian-chao</creatorcontrib><creatorcontrib>Wang, Zhu</creatorcontrib><creatorcontrib>Ruan, Si-Min</creatorcontrib><creatorcontrib>Li, Wei</creatorcontrib><creatorcontrib>Pan, Fu-Shun</creatorcontrib><creatorcontrib>Chen, Li-Da</creatorcontrib><creatorcontrib>Zhang, Yu-Chen</creatorcontrib><creatorcontrib>Wu, Wen-Xin</creatorcontrib><creatorcontrib>Xie, Xiao-Yan</creatorcontrib><creatorcontrib>Lu, Ming-De</creatorcontrib><creatorcontrib>Shan, Quan-Yuan</creatorcontrib><creatorcontrib>Wang, Wei</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Biotechnology Research Abstracts</collection><collection>Proquest Nursing & Allied Health Source</collection><collection>Neurosciences Abstracts</collection><collection>Toxicology Abstracts</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Medical Database (Alumni Edition)</collection><collection>ProQuest Pharma Collection</collection><collection>Technology Research Database</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Technology Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest Central UK/Ireland</collection><collection>Advanced Technologies & Aerospace Collection</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>ProQuest Central</collection><collection>Technology Collection</collection><collection>Natural Science Collection</collection><collection>Environmental Sciences and Pollution Management</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central Korea</collection><collection>Engineering Research Database</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>SciTech Premium Collection</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Nursing & Allied Health Database (Alumni Edition)</collection><collection>ProQuest Biological Science Collection</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Medical Database</collection><collection>Biological Science Database</collection><collection>Nursing & Allied Health Premium</collection><collection>Advanced Technologies & Aerospace Database</collection><collection>ProQuest Advanced Technologies & Aerospace Collection</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central China</collection><collection>MEDLINE - Academic</collection><jtitle>Japanese journal of radiology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Zheng, Qiao</au><au>Zhang, Jian-chao</au><au>Wang, Zhu</au><au>Ruan, Si-Min</au><au>Li, Wei</au><au>Pan, Fu-Shun</au><au>Chen, Li-Da</au><au>Zhang, Yu-Chen</au><au>Wu, Wen-Xin</au><au>Xie, Xiao-Yan</au><au>Lu, Ming-De</au><au>Shan, Quan-Yuan</au><au>Wang, Wei</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Assessment of angiogenesis in rabbit orthotropic liver tumors using three-dimensional dynamic contrast-enhanced ultrasound compared with two-dimensional DCE-US</atitle><jtitle>Japanese journal of radiology</jtitle><stitle>Jpn J Radiol</stitle><addtitle>Jpn J Radiol</addtitle><date>2019-10-01</date><risdate>2019</risdate><volume>37</volume><issue>10</issue><spage>701</spage><epage>709</epage><pages>701-709</pages><issn>1867-1071</issn><eissn>1867-108X</eissn><abstract>Objectives
To evaluate quantitative three-dimensional (3D) dynamic contrast-enhanced ultrasound (DCE-US) in the assessment of tumor angiogenesis using an orthotropic liver tumor model.
Methods
Nine New Zealand white rabbits with liver orthotropic VX2 tumors were established and imaged by two-dimensional (2D) and 3D DCE-US after SonoVue
®
bolus injections. The intraclass correlation coefficients of perfusion parameters, including peak intensity (PI), mean transit time, time to peak, and area under the curve, were calculated based on time-intensity curve. The percentage area of microvascular (PAMV) and the expression of vascular endothelial growth factor (VEGF) were both evaluated by immunohistochemical analysis and weighted by the tumor activity area ratio. Correlations between quantitative and histologic parameters were analyzed.
Results
The reproducibility of 3D DCE-US quantitative parameters was excellent (ICC 0.91–0.99); but only PI showed high reproducibility (ICC 0.97) in 2D. None of the parameters of quantitative 2D DCE-US were significantly correlated with weighted PAMV or VEGF. For 3D DCE-US, there was a positive correlation between PI and weighted PAMV (
r
= 0.74,
P
= 0.04) as well as VEGF (
r
= 0.79,
P
= 0.02).
Conclusion
Quantitative parameters of 3D DCE-US show feasibility, higher reproducibility and accuracy for the assessment of tumor angiogenesis using an orthotropic liver tumor model compared with 2D DCE-US.</abstract><cop>Tokyo</cop><pub>Springer Japan</pub><pmid>31401722</pmid><doi>10.1007/s11604-019-00861-z</doi><tpages>9</tpages><orcidid>https://orcid.org/0000-0002-9485-583X</orcidid></addata></record> |
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| ispartof | Japanese journal of radiology, 2019-10, Vol.37 (10), p.701-709 |
| issn | 1867-1071 1867-108X |
| language | eng |
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| source | MEDLINE; Springer Nature - Complete Springer Journals |
| subjects | Angiogenesis Animals Contrast Media Correlation coefficients Disease Models, Animal Drug delivery systems Feasibility studies Growth factors Humans Image Enhancement - methods Imaging Imaging, Three-Dimensional - methods Liver Liver - diagnostic imaging Liver - pathology Liver cancer Liver Neoplasms - diagnostic imaging Liver Neoplasms - pathology Mathematical models Medicine Medicine & Public Health Microvasculature Neovascularization, Pathologic - diagnostic imaging Neovascularization, Pathologic - pathology Nuclear Medicine Original Article Parameters Perfusion Rabbits Radiology Radiotherapy Reproducibility Reproducibility of Results Transit time Tumors Two dimensional models Ultrasonic imaging Ultrasonography - methods Ultrasound Vascular endothelial growth factor |
| title | Assessment of angiogenesis in rabbit orthotropic liver tumors using three-dimensional dynamic contrast-enhanced ultrasound compared with two-dimensional DCE-US |
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