Changes in saliva of dogs with canine leishmaniosis: A proteomic approach
In the present study, a quantitative proteomic approach to study changes in saliva proteins associated with canine leishmaniosis (CanL) was performed. For this, canine salivary proteins were analysed and compared between dogs before (T0) and after (T1) experimental infection with Leishmania infantum...
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| Veröffentlicht in: | Veterinary parasitology 2019-08, Vol.272, p.44-52 |
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| creator | Franco-Martínez, Lorena Tvarijonaviciute, Asta Horvatić, Anita Guillemin, Nicolas Bernal, Luis Jesús Barić Rafaj, Renata Cerón, José Joaquín Thomas, María Del Carmen López, Manuel C Tecles, Fernando Martínez-Subiela, Silvia Mrljak, Vladimir |
| description | In the present study, a quantitative proteomic approach to study changes in saliva proteins associated with canine leishmaniosis (CanL) was performed. For this, canine salivary proteins were analysed and compared between dogs before (T0) and after (T1) experimental infection with Leishmania infantum by high-throughput label-based quantitative LC-MS/MS proteomic approach and bioinformatic analysis of the in silico inferred interactome protein network was created from the initial list of differential proteins. More than 2000 proteins were identified, and of the 90 differentially expressed proteins between T0 and T1, 12 were down-regulated with log2 fold change lower than -0.5849, and 19 were up-regulated with log2 fold change greater than 0.5849. This study provides evidence of changes in salivary proteome that can occur in canine leishmaniosis and revealed biological pathways in saliva modulated in canine leishmaniosis with potential for further targeted research. |
| doi_str_mv | 10.1016/j.vetpar.2019.06.014 |
| format | Article |
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For this, canine salivary proteins were analysed and compared between dogs before (T0) and after (T1) experimental infection with Leishmania infantum by high-throughput label-based quantitative LC-MS/MS proteomic approach and bioinformatic analysis of the in silico inferred interactome protein network was created from the initial list of differential proteins. More than 2000 proteins were identified, and of the 90 differentially expressed proteins between T0 and T1, 12 were down-regulated with log2 fold change lower than -0.5849, and 19 were up-regulated with log2 fold change greater than 0.5849. This study provides evidence of changes in salivary proteome that can occur in canine leishmaniosis and revealed biological pathways in saliva modulated in canine leishmaniosis with potential for further targeted research.</description><identifier>ISSN: 0304-4017</identifier><identifier>EISSN: 1873-2550</identifier><identifier>DOI: 10.1016/j.vetpar.2019.06.014</identifier><identifier>PMID: 31395204</identifier><language>eng</language><publisher>Netherlands</publisher><subject>Animals ; Chromatography, Liquid ; Computer Simulation ; Dog Diseases - physiopathology ; Dogs ; Gene Expression Regulation ; Leishmaniasis - physiopathology ; Leishmaniasis - veterinary ; Proteome - genetics ; Proteome - metabolism ; Proteomics ; Saliva - chemistry ; Saliva - metabolism ; Salivary Proteins and Peptides - genetics ; Salivary Proteins and Peptides - metabolism ; Tandem Mass Spectrometry</subject><ispartof>Veterinary parasitology, 2019-08, Vol.272, p.44-52</ispartof><rights>Copyright © 2019 Elsevier B.V. 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This study provides evidence of changes in salivary proteome that can occur in canine leishmaniosis and revealed biological pathways in saliva modulated in canine leishmaniosis with potential for further targeted research.</description><subject>Animals</subject><subject>Chromatography, Liquid</subject><subject>Computer Simulation</subject><subject>Dog Diseases - physiopathology</subject><subject>Dogs</subject><subject>Gene Expression Regulation</subject><subject>Leishmaniasis - physiopathology</subject><subject>Leishmaniasis - veterinary</subject><subject>Proteome - genetics</subject><subject>Proteome - metabolism</subject><subject>Proteomics</subject><subject>Saliva - chemistry</subject><subject>Saliva - metabolism</subject><subject>Salivary Proteins and Peptides - genetics</subject><subject>Salivary Proteins and Peptides - metabolism</subject><subject>Tandem Mass Spectrometry</subject><issn>0304-4017</issn><issn>1873-2550</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2019</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNo9kEtPwzAQhC0EoqXwDxDykUvC2rHz4FZVPCpV4gJna-tsGld5lDgF8e9x1cJpR6uZHe3H2K2AWIBIH7bxF407HGIJooghjUGoMzYVeZZEUms4Z1NIQEUKRDZhV95vAUBBml2ySSKSQktQU7Zc1NhtyHPXcY-N-0LeV7zsN55_u7HmFjvXEW_I-boNuvfOP_I53w39SH3rLMdd0Gjra3ZRYePp5jRn7OP56X3xGq3eXpaL-Sqyic7HiFCXRKLQiiRghkFXoiLQGamwVqqidWorzNeVRStQEOY55VrnZVZIJZMZuz_eDbWfe_KjaZ231DTYUb_3RsosPKoLDcGqjlY79N4PVJnd4FocfowAc4BotuYI0RwgGkhNgBhid6eG_bql8j_0Ry35BWaXcHE</recordid><startdate>201908</startdate><enddate>201908</enddate><creator>Franco-Martínez, Lorena</creator><creator>Tvarijonaviciute, Asta</creator><creator>Horvatić, Anita</creator><creator>Guillemin, Nicolas</creator><creator>Bernal, Luis Jesús</creator><creator>Barić Rafaj, Renata</creator><creator>Cerón, José Joaquín</creator><creator>Thomas, María Del Carmen</creator><creator>López, Manuel C</creator><creator>Tecles, Fernando</creator><creator>Martínez-Subiela, Silvia</creator><creator>Mrljak, Vladimir</creator><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><orcidid>https://orcid.org/0000-0002-1524-1558</orcidid><orcidid>https://orcid.org/0000-0003-3375-4274</orcidid><orcidid>https://orcid.org/0000-0002-0723-7815</orcidid><orcidid>https://orcid.org/0000-0002-8654-1793</orcidid></search><sort><creationdate>201908</creationdate><title>Changes in saliva of dogs with canine leishmaniosis: A proteomic approach</title><author>Franco-Martínez, Lorena ; 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For this, canine salivary proteins were analysed and compared between dogs before (T0) and after (T1) experimental infection with Leishmania infantum by high-throughput label-based quantitative LC-MS/MS proteomic approach and bioinformatic analysis of the in silico inferred interactome protein network was created from the initial list of differential proteins. More than 2000 proteins were identified, and of the 90 differentially expressed proteins between T0 and T1, 12 were down-regulated with log2 fold change lower than -0.5849, and 19 were up-regulated with log2 fold change greater than 0.5849. 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| subjects | Animals Chromatography, Liquid Computer Simulation Dog Diseases - physiopathology Dogs Gene Expression Regulation Leishmaniasis - physiopathology Leishmaniasis - veterinary Proteome - genetics Proteome - metabolism Proteomics Saliva - chemistry Saliva - metabolism Salivary Proteins and Peptides - genetics Salivary Proteins and Peptides - metabolism Tandem Mass Spectrometry |
| title | Changes in saliva of dogs with canine leishmaniosis: A proteomic approach |
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