Pinosylvin reduced migration and invasion of oral cancer carcinoma by regulating matrix metalloproteinase-2 expression and extracellular signal-regulated kinase pathway
•Pinosylvin restrained the migration and invasion of oral cancer cells.•Pinosylvin reduced the enzyme activity of MMP-2 and increased the TIMP-2 expression in oral cancer cells.•Pinosylvin inhibited the ERK pathway in human oral cancer cells.•ERK signaling pathway in pinosylvin-mediated suppression...
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| Veröffentlicht in: | Biomedicine & pharmacotherapy 2019-09, Vol.117, p.109160-109160, Article 109160 |
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| container_title | Biomedicine & pharmacotherapy |
| container_volume | 117 |
| creator | Chen, Mu-Kuan Liu, Yen-Tze Lin, Jen-Tsun Lin, Chia-Chieh Chuang, Yi-Ching Lo, Yu-Sheng Hsi, Yi-Ting Hsieh, Ming-Ju |
| description | •Pinosylvin restrained the migration and invasion of oral cancer cells.•Pinosylvin reduced the enzyme activity of MMP-2 and increased the TIMP-2 expression in oral cancer cells.•Pinosylvin inhibited the ERK pathway in human oral cancer cells.•ERK signaling pathway in pinosylvin-mediated suppression of OSCC cells.
[Display omitted]
Pinosylvin possesses several biological properties, including anti-inflammatory, antitumor, and antioxidant characteristics. However, the effects of pinosylvin on the migration and invasion of human oral cancer cells and the underlying mechanisms remain unclear.
In this research, we investigated the outcome of different concentrations of pinosylvin (0–80 μM) on the metastatic and invasive abilities of SAS, SCC-9, and HSC-3 cells.
Western blotting assay and Gelatin zymography assay indicated that pinosylvin inhibited the enzymatic activity of matrix metalloproteinase-2 (MMP-2) and reduced its protein level but increased the expression of tissue inhibitor of metalloproteinase-2 (TIMP-2). Additionally, the wound healing assay and Transwell method showed that pinosylvin reduced the migration of SAS, SCC-9 and HSC-3 oral cancer cells. Besides, pinosylvin decreased the phosphorylation of ERK1/2 protein experssion in both SAS and SCC-9 cells.
These results indicate that pinosylvin is a potential anticancer agent for preventing oral cancer metastasis. |
| doi_str_mv | 10.1016/j.biopha.2019.109160 |
| format | Article |
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[Display omitted]
Pinosylvin possesses several biological properties, including anti-inflammatory, antitumor, and antioxidant characteristics. However, the effects of pinosylvin on the migration and invasion of human oral cancer cells and the underlying mechanisms remain unclear.
In this research, we investigated the outcome of different concentrations of pinosylvin (0–80 μM) on the metastatic and invasive abilities of SAS, SCC-9, and HSC-3 cells.
Western blotting assay and Gelatin zymography assay indicated that pinosylvin inhibited the enzymatic activity of matrix metalloproteinase-2 (MMP-2) and reduced its protein level but increased the expression of tissue inhibitor of metalloproteinase-2 (TIMP-2). Additionally, the wound healing assay and Transwell method showed that pinosylvin reduced the migration of SAS, SCC-9 and HSC-3 oral cancer cells. Besides, pinosylvin decreased the phosphorylation of ERK1/2 protein experssion in both SAS and SCC-9 cells.
These results indicate that pinosylvin is a potential anticancer agent for preventing oral cancer metastasis.</description><identifier>ISSN: 0753-3322</identifier><identifier>EISSN: 1950-6007</identifier><identifier>DOI: 10.1016/j.biopha.2019.109160</identifier><identifier>PMID: 31387166</identifier><language>eng</language><publisher>France: Elsevier Masson SAS</publisher><subject>Carcinoma, Squamous Cell - drug therapy ; Carcinoma, Squamous Cell - metabolism ; Cell Line, Tumor ; Cell Movement - drug effects ; Extracellular Signal-Regulated MAP Kinases - metabolism ; Humans ; MAP Kinase Signaling System - drug effects ; Matrix Metalloproteinase 2 - metabolism ; Matrix Metalloproteinase Inhibitors - pharmacology ; Migration ; MMP-2 ; Mouth Neoplasms - drug therapy ; Mouth Neoplasms - metabolism ; Neoplasm Invasiveness - pathology ; Oral squamous cell carcinoma ; Phosphorylation - drug effects ; Pinosylvin ; Signal Transduction - drug effects ; Stilbenes - pharmacology ; TIMP-2 ; Tissue Inhibitor of Metalloproteinase-2 - metabolism</subject><ispartof>Biomedicine & pharmacotherapy, 2019-09, Vol.117, p.109160-109160, Article 109160</ispartof><rights>2019</rights><rights>Copyright © 2019. Published by Elsevier Masson SAS.</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c408t-baeb382187a1a533f1420c60f6ccc1cafa316df88611ca2e4eac825efae1fa5c3</citedby><cites>FETCH-LOGICAL-c408t-baeb382187a1a533f1420c60f6ccc1cafa316df88611ca2e4eac825efae1fa5c3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://www.sciencedirect.com/science/article/pii/S0753332219324783$$EHTML$$P50$$Gelsevier$$Hfree_for_read</linktohtml><link.rule.ids>314,776,780,3537,27901,27902,65306</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/31387166$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Chen, Mu-Kuan</creatorcontrib><creatorcontrib>Liu, Yen-Tze</creatorcontrib><creatorcontrib>Lin, Jen-Tsun</creatorcontrib><creatorcontrib>Lin, Chia-Chieh</creatorcontrib><creatorcontrib>Chuang, Yi-Ching</creatorcontrib><creatorcontrib>Lo, Yu-Sheng</creatorcontrib><creatorcontrib>Hsi, Yi-Ting</creatorcontrib><creatorcontrib>Hsieh, Ming-Ju</creatorcontrib><title>Pinosylvin reduced migration and invasion of oral cancer carcinoma by regulating matrix metalloproteinase-2 expression and extracellular signal-regulated kinase pathway</title><title>Biomedicine & pharmacotherapy</title><addtitle>Biomed Pharmacother</addtitle><description>•Pinosylvin restrained the migration and invasion of oral cancer cells.•Pinosylvin reduced the enzyme activity of MMP-2 and increased the TIMP-2 expression in oral cancer cells.•Pinosylvin inhibited the ERK pathway in human oral cancer cells.•ERK signaling pathway in pinosylvin-mediated suppression of OSCC cells.
[Display omitted]
Pinosylvin possesses several biological properties, including anti-inflammatory, antitumor, and antioxidant characteristics. However, the effects of pinosylvin on the migration and invasion of human oral cancer cells and the underlying mechanisms remain unclear.
In this research, we investigated the outcome of different concentrations of pinosylvin (0–80 μM) on the metastatic and invasive abilities of SAS, SCC-9, and HSC-3 cells.
Western blotting assay and Gelatin zymography assay indicated that pinosylvin inhibited the enzymatic activity of matrix metalloproteinase-2 (MMP-2) and reduced its protein level but increased the expression of tissue inhibitor of metalloproteinase-2 (TIMP-2). Additionally, the wound healing assay and Transwell method showed that pinosylvin reduced the migration of SAS, SCC-9 and HSC-3 oral cancer cells. Besides, pinosylvin decreased the phosphorylation of ERK1/2 protein experssion in both SAS and SCC-9 cells.
These results indicate that pinosylvin is a potential anticancer agent for preventing oral cancer metastasis.</description><subject>Carcinoma, Squamous Cell - drug therapy</subject><subject>Carcinoma, Squamous Cell - metabolism</subject><subject>Cell Line, Tumor</subject><subject>Cell Movement - drug effects</subject><subject>Extracellular Signal-Regulated MAP Kinases - metabolism</subject><subject>Humans</subject><subject>MAP Kinase Signaling System - drug effects</subject><subject>Matrix Metalloproteinase 2 - metabolism</subject><subject>Matrix Metalloproteinase Inhibitors - pharmacology</subject><subject>Migration</subject><subject>MMP-2</subject><subject>Mouth Neoplasms - drug therapy</subject><subject>Mouth Neoplasms - metabolism</subject><subject>Neoplasm Invasiveness - pathology</subject><subject>Oral squamous cell carcinoma</subject><subject>Phosphorylation - drug effects</subject><subject>Pinosylvin</subject><subject>Signal Transduction - drug effects</subject><subject>Stilbenes - pharmacology</subject><subject>TIMP-2</subject><subject>Tissue Inhibitor of Metalloproteinase-2 - metabolism</subject><issn>0753-3322</issn><issn>1950-6007</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2019</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9kU2P0zAQhi0EYsvCP0DIRy7p-iNx0wsSWvGx0kpwgLM1cSZdF8cOtlPaf8TPxCVdjpxGY73PzDt-CXnN2Zozrm72686G6QHWgvFtedpyxZ6QFd82rFKMbZ6SFds0spJSiCvyIqU9Y6xRsn1OriSX7YYrtSK_v1of0skdrKcR-9lgT0e7i5Bt8BR8T60_QDo3YaAhgqMGvMFYSjSFHYF2p4LuZlcYv6Mj5GiPdMQMzoUphozWQ8JKUDxOEVN6nIzHHMGgcwWNNNmdB1ddJhUbP_5idIL88AtOL8mzAVzCV5d6Tb5__PDt9nN1_-XT3e37-8rUrM1VB9jJVvB2AxwaKQdeC2YUG5QxhhsYQHLVD22reOkE1gimFQ0OgHyAxshr8naZW5z_nDFlPdp0Ngkew5y0EGpbM1ErXqT1IjUxpBRx0FO0I8ST5kyfM9J7vWSkzxnpJaOCvblsmLsR-3_QYyhF8G4RYLnzYDHqZCyWT-9tRJN1H-z_N_wBv-aqqQ</recordid><startdate>201909</startdate><enddate>201909</enddate><creator>Chen, Mu-Kuan</creator><creator>Liu, Yen-Tze</creator><creator>Lin, Jen-Tsun</creator><creator>Lin, Chia-Chieh</creator><creator>Chuang, Yi-Ching</creator><creator>Lo, Yu-Sheng</creator><creator>Hsi, Yi-Ting</creator><creator>Hsieh, Ming-Ju</creator><general>Elsevier Masson SAS</general><scope>6I.</scope><scope>AAFTH</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>201909</creationdate><title>Pinosylvin reduced migration and invasion of oral cancer carcinoma by regulating matrix metalloproteinase-2 expression and extracellular signal-regulated kinase pathway</title><author>Chen, Mu-Kuan ; Liu, Yen-Tze ; Lin, Jen-Tsun ; Lin, Chia-Chieh ; Chuang, Yi-Ching ; Lo, Yu-Sheng ; Hsi, Yi-Ting ; Hsieh, Ming-Ju</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c408t-baeb382187a1a533f1420c60f6ccc1cafa316df88611ca2e4eac825efae1fa5c3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2019</creationdate><topic>Carcinoma, Squamous Cell - drug therapy</topic><topic>Carcinoma, Squamous Cell - metabolism</topic><topic>Cell Line, Tumor</topic><topic>Cell Movement - drug effects</topic><topic>Extracellular Signal-Regulated MAP Kinases - metabolism</topic><topic>Humans</topic><topic>MAP Kinase Signaling System - drug effects</topic><topic>Matrix Metalloproteinase 2 - metabolism</topic><topic>Matrix Metalloproteinase Inhibitors - pharmacology</topic><topic>Migration</topic><topic>MMP-2</topic><topic>Mouth Neoplasms - drug therapy</topic><topic>Mouth Neoplasms - metabolism</topic><topic>Neoplasm Invasiveness - pathology</topic><topic>Oral squamous cell carcinoma</topic><topic>Phosphorylation - drug effects</topic><topic>Pinosylvin</topic><topic>Signal Transduction - drug effects</topic><topic>Stilbenes - pharmacology</topic><topic>TIMP-2</topic><topic>Tissue Inhibitor of Metalloproteinase-2 - metabolism</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Chen, Mu-Kuan</creatorcontrib><creatorcontrib>Liu, Yen-Tze</creatorcontrib><creatorcontrib>Lin, Jen-Tsun</creatorcontrib><creatorcontrib>Lin, Chia-Chieh</creatorcontrib><creatorcontrib>Chuang, Yi-Ching</creatorcontrib><creatorcontrib>Lo, Yu-Sheng</creatorcontrib><creatorcontrib>Hsi, Yi-Ting</creatorcontrib><creatorcontrib>Hsieh, Ming-Ju</creatorcontrib><collection>ScienceDirect Open Access Titles</collection><collection>Elsevier:ScienceDirect:Open Access</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Biomedicine & pharmacotherapy</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Chen, Mu-Kuan</au><au>Liu, Yen-Tze</au><au>Lin, Jen-Tsun</au><au>Lin, Chia-Chieh</au><au>Chuang, Yi-Ching</au><au>Lo, Yu-Sheng</au><au>Hsi, Yi-Ting</au><au>Hsieh, Ming-Ju</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Pinosylvin reduced migration and invasion of oral cancer carcinoma by regulating matrix metalloproteinase-2 expression and extracellular signal-regulated kinase pathway</atitle><jtitle>Biomedicine & pharmacotherapy</jtitle><addtitle>Biomed Pharmacother</addtitle><date>2019-09</date><risdate>2019</risdate><volume>117</volume><spage>109160</spage><epage>109160</epage><pages>109160-109160</pages><artnum>109160</artnum><issn>0753-3322</issn><eissn>1950-6007</eissn><abstract>•Pinosylvin restrained the migration and invasion of oral cancer cells.•Pinosylvin reduced the enzyme activity of MMP-2 and increased the TIMP-2 expression in oral cancer cells.•Pinosylvin inhibited the ERK pathway in human oral cancer cells.•ERK signaling pathway in pinosylvin-mediated suppression of OSCC cells.
[Display omitted]
Pinosylvin possesses several biological properties, including anti-inflammatory, antitumor, and antioxidant characteristics. However, the effects of pinosylvin on the migration and invasion of human oral cancer cells and the underlying mechanisms remain unclear.
In this research, we investigated the outcome of different concentrations of pinosylvin (0–80 μM) on the metastatic and invasive abilities of SAS, SCC-9, and HSC-3 cells.
Western blotting assay and Gelatin zymography assay indicated that pinosylvin inhibited the enzymatic activity of matrix metalloproteinase-2 (MMP-2) and reduced its protein level but increased the expression of tissue inhibitor of metalloproteinase-2 (TIMP-2). Additionally, the wound healing assay and Transwell method showed that pinosylvin reduced the migration of SAS, SCC-9 and HSC-3 oral cancer cells. Besides, pinosylvin decreased the phosphorylation of ERK1/2 protein experssion in both SAS and SCC-9 cells.
These results indicate that pinosylvin is a potential anticancer agent for preventing oral cancer metastasis.</abstract><cop>France</cop><pub>Elsevier Masson SAS</pub><pmid>31387166</pmid><doi>10.1016/j.biopha.2019.109160</doi><tpages>1</tpages><oa>free_for_read</oa></addata></record> |
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| ispartof | Biomedicine & pharmacotherapy, 2019-09, Vol.117, p.109160-109160, Article 109160 |
| issn | 0753-3322 1950-6007 |
| language | eng |
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| source | MEDLINE; Elsevier ScienceDirect Journals Complete; EZB Electronic Journals Library |
| subjects | Carcinoma, Squamous Cell - drug therapy Carcinoma, Squamous Cell - metabolism Cell Line, Tumor Cell Movement - drug effects Extracellular Signal-Regulated MAP Kinases - metabolism Humans MAP Kinase Signaling System - drug effects Matrix Metalloproteinase 2 - metabolism Matrix Metalloproteinase Inhibitors - pharmacology Migration MMP-2 Mouth Neoplasms - drug therapy Mouth Neoplasms - metabolism Neoplasm Invasiveness - pathology Oral squamous cell carcinoma Phosphorylation - drug effects Pinosylvin Signal Transduction - drug effects Stilbenes - pharmacology TIMP-2 Tissue Inhibitor of Metalloproteinase-2 - metabolism |
| title | Pinosylvin reduced migration and invasion of oral cancer carcinoma by regulating matrix metalloproteinase-2 expression and extracellular signal-regulated kinase pathway |
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