Molecular characterization of the influenza A(H1N1)pdm09 isolates collected in the 2015‐2016 season and comparison of HA mutations detected in Turkey since 2009
Influenza A(H1N1)pdm09 pandemic virus causing the 2009 global outbreak moved into the post‐pandemic period, but its variants continued to be the prevailing subtype in the 2015‐2016 influenza season in Europe and Asia. To determine the molecular characteristics of influenza A(H1N1)pdm09 isolates circ...
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| Veröffentlicht in: | Journal of medical virology 2019-12, Vol.91 (12), p.2074-2082 |
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| creator | Guldemir, Dilek Coskun‐Ari, Fatma Filiz Altas, Ayse Basak Bakkaloglu, Zekiye Unaldi, Ozlem Bayraktar, Fatma Korukluoglu, Gulay Aktas, Ali Riza Durmaz, Riza |
| description | Influenza A(H1N1)pdm09 pandemic virus causing the 2009 global outbreak moved into the post‐pandemic period, but its variants continued to be the prevailing subtype in the 2015‐2016 influenza season in Europe and Asia. To determine the molecular characteristics of influenza A(H1N1)pdm09 isolates circulating during the 2015‐2016 season in Turkey, we identified mutations in the hemagglutinin (HA) genes and investigated the presence of H275Y alteration in the neuraminidase genes in the randomly selected isolates. The comparison of the HA nucleotide sequences revealed a very high homology (>99.5%) among the studied influenza A(H1N1)pdm09 isolates, while a relatively low homology (96.6%‐97.2%), was observed between Turkish isolates and the A/California/07/2009 vaccine virus. Overall 14 common mutations were detected in HA sequences of all 2015‐2016 influenza A(H1N1)pdm09 isolates with respect to the A/California/07/2009 virus, four of which located in three different antigenic sites. Eleven rare mutations in 12 HA sequences were also detected. Phylogenetic analysis revealed that all characterized influenza A(H1N1)pdm09 isolates formed a single genetic cluster, belonging to the genetic subclade 6B.1, defined by HA amino acid substitutions S84N, S162N, and I216T. Furthermore, all isolates showed an oseltamivir‐sensitive genotype, suggesting that Tamiflu (Oseltamivir) could still be the drug of choice in Turkey. |
| doi_str_mv | 10.1002/jmv.25565 |
| format | Article |
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To determine the molecular characteristics of influenza A(H1N1)pdm09 isolates circulating during the 2015‐2016 season in Turkey, we identified mutations in the hemagglutinin (HA) genes and investigated the presence of H275Y alteration in the neuraminidase genes in the randomly selected isolates. The comparison of the HA nucleotide sequences revealed a very high homology (>99.5%) among the studied influenza A(H1N1)pdm09 isolates, while a relatively low homology (96.6%‐97.2%), was observed between Turkish isolates and the A/California/07/2009 vaccine virus. Overall 14 common mutations were detected in HA sequences of all 2015‐2016 influenza A(H1N1)pdm09 isolates with respect to the A/California/07/2009 virus, four of which located in three different antigenic sites. Eleven rare mutations in 12 HA sequences were also detected. Phylogenetic analysis revealed that all characterized influenza A(H1N1)pdm09 isolates formed a single genetic cluster, belonging to the genetic subclade 6B.1, defined by HA amino acid substitutions S84N, S162N, and I216T. Furthermore, all isolates showed an oseltamivir‐sensitive genotype, suggesting that Tamiflu (Oseltamivir) could still be the drug of choice in Turkey.</description><identifier>ISSN: 0146-6615</identifier><identifier>EISSN: 1096-9071</identifier><identifier>DOI: 10.1002/jmv.25565</identifier><identifier>PMID: 31389035</identifier><language>eng</language><publisher>United States: Wiley Subscription Services, Inc</publisher><subject>2015‐2016 influenza season ; Amino acids ; Antigens ; Antiviral drugs ; Exo-a-sialidase ; Genes ; Genotypes ; hemagglutinin ; Hemagglutinins ; Homology ; Influenza ; Influenza A ; influenza A(H1N1)pdm09 ; Mutation ; neuraminidase ; Nucleotides ; Oseltamivir ; oseltamivir resistance ; Pandemics ; Phylogeny ; Vaccines ; Virology ; Viruses</subject><ispartof>Journal of medical virology, 2019-12, Vol.91 (12), p.2074-2082</ispartof><rights>2019 Wiley Periodicals, Inc.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c3535-f73a705ae2eb4f107bd6b31da383f9e2d84c7de50a393ed79e07a6f26411e00d3</citedby><cites>FETCH-LOGICAL-c3535-f73a705ae2eb4f107bd6b31da383f9e2d84c7de50a393ed79e07a6f26411e00d3</cites><orcidid>0000-0001-6561-778X ; 0000-0002-8107-732X</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1002%2Fjmv.25565$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1002%2Fjmv.25565$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>314,776,780,1411,27901,27902,45550,45551</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/31389035$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Guldemir, Dilek</creatorcontrib><creatorcontrib>Coskun‐Ari, Fatma Filiz</creatorcontrib><creatorcontrib>Altas, Ayse Basak</creatorcontrib><creatorcontrib>Bakkaloglu, Zekiye</creatorcontrib><creatorcontrib>Unaldi, Ozlem</creatorcontrib><creatorcontrib>Bayraktar, Fatma</creatorcontrib><creatorcontrib>Korukluoglu, Gulay</creatorcontrib><creatorcontrib>Aktas, Ali Riza</creatorcontrib><creatorcontrib>Durmaz, Riza</creatorcontrib><title>Molecular characterization of the influenza A(H1N1)pdm09 isolates collected in the 2015‐2016 season and comparison of HA mutations detected in Turkey since 2009</title><title>Journal of medical virology</title><addtitle>J Med Virol</addtitle><description>Influenza A(H1N1)pdm09 pandemic virus causing the 2009 global outbreak moved into the post‐pandemic period, but its variants continued to be the prevailing subtype in the 2015‐2016 influenza season in Europe and Asia. To determine the molecular characteristics of influenza A(H1N1)pdm09 isolates circulating during the 2015‐2016 season in Turkey, we identified mutations in the hemagglutinin (HA) genes and investigated the presence of H275Y alteration in the neuraminidase genes in the randomly selected isolates. The comparison of the HA nucleotide sequences revealed a very high homology (>99.5%) among the studied influenza A(H1N1)pdm09 isolates, while a relatively low homology (96.6%‐97.2%), was observed between Turkish isolates and the A/California/07/2009 vaccine virus. Overall 14 common mutations were detected in HA sequences of all 2015‐2016 influenza A(H1N1)pdm09 isolates with respect to the A/California/07/2009 virus, four of which located in three different antigenic sites. Eleven rare mutations in 12 HA sequences were also detected. Phylogenetic analysis revealed that all characterized influenza A(H1N1)pdm09 isolates formed a single genetic cluster, belonging to the genetic subclade 6B.1, defined by HA amino acid substitutions S84N, S162N, and I216T. Furthermore, all isolates showed an oseltamivir‐sensitive genotype, suggesting that Tamiflu (Oseltamivir) could still be the drug of choice in Turkey.</description><subject>2015‐2016 influenza season</subject><subject>Amino acids</subject><subject>Antigens</subject><subject>Antiviral drugs</subject><subject>Exo-a-sialidase</subject><subject>Genes</subject><subject>Genotypes</subject><subject>hemagglutinin</subject><subject>Hemagglutinins</subject><subject>Homology</subject><subject>Influenza</subject><subject>Influenza A</subject><subject>influenza A(H1N1)pdm09</subject><subject>Mutation</subject><subject>neuraminidase</subject><subject>Nucleotides</subject><subject>Oseltamivir</subject><subject>oseltamivir resistance</subject><subject>Pandemics</subject><subject>Phylogeny</subject><subject>Vaccines</subject><subject>Virology</subject><subject>Viruses</subject><issn>0146-6615</issn><issn>1096-9071</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2019</creationdate><recordtype>article</recordtype><recordid>eNp10UFu1DAYhmELgehQWHABZIlNu0j7247teDmqgAG1sClsLU_8R83gJIOdFE1XHIEzcLSepO6kdIHEypvHry19hLxmcMIA-Ommuz7hUir5hCwYGFUY0OwpWQArVaEUkwfkRUobAKgM58_JgWCiMiDkgvy5GALWU3CR1lcuunrE2N64sR16OjR0vELa9k2YsL9xdHm0Yp_Z8dZ3YGibhuBGTLQeQk6M6LPcX-DA5O2v3_lQNKFLOeV6n123dbFNc3m1pN007h9K1OP4WLic4nfc0dT29X0KzEvyrHEh4auH85B8ff_u8mxVnH_58PFseV7UQgpZNFo4DdIhx3XZMNBrr9aCeScq0Rjkvipr7VGCE0ag1wZBO9VwVTKGAF4ckqO5u43DjwnTaLs21RiC63GYkuVcGWEkMJbp23_oZphin39nuQBZlVIrndXxrOo4pBSxsdvYdi7uLAN7P5zNw9n9cNm-eShO6w79o_y7VAanM_jZBtz9v2Q_XXybk3fdTaHl</recordid><startdate>201912</startdate><enddate>201912</enddate><creator>Guldemir, Dilek</creator><creator>Coskun‐Ari, Fatma Filiz</creator><creator>Altas, Ayse Basak</creator><creator>Bakkaloglu, Zekiye</creator><creator>Unaldi, Ozlem</creator><creator>Bayraktar, Fatma</creator><creator>Korukluoglu, Gulay</creator><creator>Aktas, Ali Riza</creator><creator>Durmaz, Riza</creator><general>Wiley Subscription Services, Inc</general><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QL</scope><scope>7TK</scope><scope>7U9</scope><scope>8FD</scope><scope>C1K</scope><scope>FR3</scope><scope>H94</scope><scope>K9.</scope><scope>M7N</scope><scope>P64</scope><scope>RC3</scope><scope>7X8</scope><orcidid>https://orcid.org/0000-0001-6561-778X</orcidid><orcidid>https://orcid.org/0000-0002-8107-732X</orcidid></search><sort><creationdate>201912</creationdate><title>Molecular characterization of the influenza A(H1N1)pdm09 isolates collected in the 2015‐2016 season and comparison of HA mutations detected in Turkey since 2009</title><author>Guldemir, Dilek ; Coskun‐Ari, Fatma Filiz ; Altas, Ayse Basak ; Bakkaloglu, Zekiye ; Unaldi, Ozlem ; Bayraktar, Fatma ; Korukluoglu, Gulay ; Aktas, Ali Riza ; Durmaz, Riza</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c3535-f73a705ae2eb4f107bd6b31da383f9e2d84c7de50a393ed79e07a6f26411e00d3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2019</creationdate><topic>2015‐2016 influenza season</topic><topic>Amino acids</topic><topic>Antigens</topic><topic>Antiviral drugs</topic><topic>Exo-a-sialidase</topic><topic>Genes</topic><topic>Genotypes</topic><topic>hemagglutinin</topic><topic>Hemagglutinins</topic><topic>Homology</topic><topic>Influenza</topic><topic>Influenza A</topic><topic>influenza A(H1N1)pdm09</topic><topic>Mutation</topic><topic>neuraminidase</topic><topic>Nucleotides</topic><topic>Oseltamivir</topic><topic>oseltamivir resistance</topic><topic>Pandemics</topic><topic>Phylogeny</topic><topic>Vaccines</topic><topic>Virology</topic><topic>Viruses</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Guldemir, Dilek</creatorcontrib><creatorcontrib>Coskun‐Ari, Fatma Filiz</creatorcontrib><creatorcontrib>Altas, Ayse Basak</creatorcontrib><creatorcontrib>Bakkaloglu, Zekiye</creatorcontrib><creatorcontrib>Unaldi, Ozlem</creatorcontrib><creatorcontrib>Bayraktar, Fatma</creatorcontrib><creatorcontrib>Korukluoglu, Gulay</creatorcontrib><creatorcontrib>Aktas, Ali Riza</creatorcontrib><creatorcontrib>Durmaz, Riza</creatorcontrib><collection>PubMed</collection><collection>CrossRef</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Neurosciences Abstracts</collection><collection>Virology and AIDS Abstracts</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>Engineering Research Database</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Genetics Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Journal of medical virology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Guldemir, Dilek</au><au>Coskun‐Ari, Fatma Filiz</au><au>Altas, Ayse Basak</au><au>Bakkaloglu, Zekiye</au><au>Unaldi, Ozlem</au><au>Bayraktar, Fatma</au><au>Korukluoglu, Gulay</au><au>Aktas, Ali Riza</au><au>Durmaz, Riza</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Molecular characterization of the influenza A(H1N1)pdm09 isolates collected in the 2015‐2016 season and comparison of HA mutations detected in Turkey since 2009</atitle><jtitle>Journal of medical virology</jtitle><addtitle>J Med Virol</addtitle><date>2019-12</date><risdate>2019</risdate><volume>91</volume><issue>12</issue><spage>2074</spage><epage>2082</epage><pages>2074-2082</pages><issn>0146-6615</issn><eissn>1096-9071</eissn><abstract>Influenza A(H1N1)pdm09 pandemic virus causing the 2009 global outbreak moved into the post‐pandemic period, but its variants continued to be the prevailing subtype in the 2015‐2016 influenza season in Europe and Asia. To determine the molecular characteristics of influenza A(H1N1)pdm09 isolates circulating during the 2015‐2016 season in Turkey, we identified mutations in the hemagglutinin (HA) genes and investigated the presence of H275Y alteration in the neuraminidase genes in the randomly selected isolates. The comparison of the HA nucleotide sequences revealed a very high homology (>99.5%) among the studied influenza A(H1N1)pdm09 isolates, while a relatively low homology (96.6%‐97.2%), was observed between Turkish isolates and the A/California/07/2009 vaccine virus. Overall 14 common mutations were detected in HA sequences of all 2015‐2016 influenza A(H1N1)pdm09 isolates with respect to the A/California/07/2009 virus, four of which located in three different antigenic sites. Eleven rare mutations in 12 HA sequences were also detected. Phylogenetic analysis revealed that all characterized influenza A(H1N1)pdm09 isolates formed a single genetic cluster, belonging to the genetic subclade 6B.1, defined by HA amino acid substitutions S84N, S162N, and I216T. Furthermore, all isolates showed an oseltamivir‐sensitive genotype, suggesting that Tamiflu (Oseltamivir) could still be the drug of choice in Turkey.</abstract><cop>United States</cop><pub>Wiley Subscription Services, Inc</pub><pmid>31389035</pmid><doi>10.1002/jmv.25565</doi><tpages>9</tpages><orcidid>https://orcid.org/0000-0001-6561-778X</orcidid><orcidid>https://orcid.org/0000-0002-8107-732X</orcidid></addata></record> |
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| subjects | 2015‐2016 influenza season Amino acids Antigens Antiviral drugs Exo-a-sialidase Genes Genotypes hemagglutinin Hemagglutinins Homology Influenza Influenza A influenza A(H1N1)pdm09 Mutation neuraminidase Nucleotides Oseltamivir oseltamivir resistance Pandemics Phylogeny Vaccines Virology Viruses |
| title | Molecular characterization of the influenza A(H1N1)pdm09 isolates collected in the 2015‐2016 season and comparison of HA mutations detected in Turkey since 2009 |
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