A simple method for the isolation and detailed characterization of primary human proximal tubule cells for renal replacement therapy

The main physiological functions of renal proximal tubule cells in vivo are reabsorption of essential nutrients from the glomerular filtrate and secretion of waste products and xenobiotics into urine. Currently, there are several established cell lines of human origin available as in vitro models of...

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Veröffentlicht in:	International journal of artificial organs 2020-01, Vol.43 (1), p.45-57
Hauptverfasser:	Sánchez-Romero, Natalia, Martínez-Gimeno, Laura, Caetano-Pinto, Pedro, Saez, Berta, Sánchez-Zalabardo, José Manuel, Masereeuw, Rosalinde, Giménez, Ignacio
Format:	Artikel
Sprache:	eng
Schlagworte:	ATP Binding Cassette Transporter, Subfamily B - metabolism ATP Binding Cassette Transporter, Subfamily B, Member 1 - metabolism ATP Binding Cassette Transporter, Subfamily G, Member 2 - metabolism Breast cancer Cell culture Cell Culture Techniques - methods Cilia Dipeptidyl Peptidase 4 - metabolism Dipeptidyl-peptidase IV Enzymatic activity Essential nutrients Filtrate gamma-Glutamyltransferase - metabolism Glycoproteins Humans Kidney Tubules, Proximal - cytology Kidneys Markers Mimicry Multidrug resistance Na+/glucose cotransporter Neoplasm Proteins - metabolism Nephrectomy Nutrients Organic Anion Transporters - metabolism P-Glycoprotein Peptidase Proteins Reabsorption Regenerative medicine Renal cell carcinoma Renal function Renal Replacement Therapy Sodium Tight junctions Tissue engineering Urine Xenobiotics
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