In vitro study of the cytotoxicity of thymoquinone/curcumin fluorescent liposomes
In the present study, thymoquinone-loaded liposomes (Lip (TQ)), curcumin-encapsulated liposome (Lip (CUR)), and thymoquinone/curcumin-encapsulated liposome (Lip (TQ + CUR)) in addition to rhodamine-labeled thymoquinone/curcumin liposome (Lip (TQ + CUR + ROD)) were prepared with encapsulation efficie...
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| Veröffentlicht in: | Naunyn-Schmiedeberg's archives of pharmacology 2019-11, Vol.392 (11), p.1465-1476 |
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| description | In the present study, thymoquinone-loaded liposomes (Lip (TQ)), curcumin-encapsulated liposome (Lip (CUR)), and thymoquinone/curcumin-encapsulated liposome (Lip (TQ + CUR)) in addition to rhodamine-labeled thymoquinone/curcumin liposome (Lip (TQ + CUR + ROD)) were prepared with encapsulation efficiency exceeding 99%. The aim of the present study was to evaluate the effect of the different prepared formulations either labeled with the fluorescent dye (rhodamine B) or not on A549 lung cancer cells. Cytotoxicity of different formulations was assessed by MTT assay. Proliferation of A549 cells was significantly inhibited by the different formulations in a concentration-dependent manner in 72 h. The Lip (TQ + CUR + ROD) formulation demonstrated the lowest IC
50
value. To investigate its mechanism of action on A549 lung cancer cells, the Comet assay (for DNA damage) was done, the measurement of some oxidative stress parameters in addition to performing inverted fluorescence microscopy imaging. The results of the present study demonstrated the increased DNA damage, oxidative stress damage, and cell apoptosis in A549 treated with TQ, CUR, and rhodamine-encapsulated fluorescent liposome formulation as compared to untreated cells. The results obtained from the present study demonstrate the significant role of the TQ/CUR fluorescent liposomes on decreasing the viability of A549 lung cancer cells.
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| doi_str_mv | 10.1007/s00210-019-01688-1 |
| format | Article |
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50
value. To investigate its mechanism of action on A549 lung cancer cells, the Comet assay (for DNA damage) was done, the measurement of some oxidative stress parameters in addition to performing inverted fluorescence microscopy imaging. The results of the present study demonstrated the increased DNA damage, oxidative stress damage, and cell apoptosis in A549 treated with TQ, CUR, and rhodamine-encapsulated fluorescent liposome formulation as compared to untreated cells. The results obtained from the present study demonstrate the significant role of the TQ/CUR fluorescent liposomes on decreasing the viability of A549 lung cancer cells.
Graphical abstract</description><identifier>ISSN: 0028-1298</identifier><identifier>EISSN: 1432-1912</identifier><identifier>DOI: 10.1007/s00210-019-01688-1</identifier><identifier>PMID: 31377882</identifier><language>eng</language><publisher>Berlin/Heidelberg: Springer Berlin Heidelberg</publisher><subject>Apoptosis ; Biomedical and Life Sciences ; Biomedicine ; Cell proliferation ; Comet assay ; Curcumin ; Cytotoxicity ; Deoxyribonucleic acid ; DNA ; DNA damage ; Encapsulation ; Fluorescence ; Fluorescence microscopy ; Fluorescent dyes ; Fluorescent indicators ; Formulations ; Liposomes ; Lung cancer ; Neurosciences ; Original Article ; Oxidative stress ; Pharmacology/Toxicology ; Rhodamine ; Viability</subject><ispartof>Naunyn-Schmiedeberg's archives of pharmacology, 2019-11, Vol.392 (11), p.1465-1476</ispartof><rights>Springer-Verlag GmbH Germany, part of Springer Nature 2019</rights><rights>Naunyn-Schmiedeberg's Archives of Pharmacology is a copyright of Springer, (2019). All Rights Reserved.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c375t-1765a392791133c9e8ed69f0a386b800210460cedd3b2570d1c5ac3f644c99f83</citedby><cites>FETCH-LOGICAL-c375t-1765a392791133c9e8ed69f0a386b800210460cedd3b2570d1c5ac3f644c99f83</cites><orcidid>0000-0001-8689-1198</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://link.springer.com/content/pdf/10.1007/s00210-019-01688-1$$EPDF$$P50$$Gspringer$$H</linktopdf><linktohtml>$$Uhttps://link.springer.com/10.1007/s00210-019-01688-1$$EHTML$$P50$$Gspringer$$H</linktohtml><link.rule.ids>314,776,780,27901,27902,41464,42533,51294</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/31377882$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Fahmy, Heba Mohamed</creatorcontrib><title>In vitro study of the cytotoxicity of thymoquinone/curcumin fluorescent liposomes</title><title>Naunyn-Schmiedeberg's archives of pharmacology</title><addtitle>Naunyn-Schmiedeberg's Arch Pharmacol</addtitle><addtitle>Naunyn Schmiedebergs Arch Pharmacol</addtitle><description>In the present study, thymoquinone-loaded liposomes (Lip (TQ)), curcumin-encapsulated liposome (Lip (CUR)), and thymoquinone/curcumin-encapsulated liposome (Lip (TQ + CUR)) in addition to rhodamine-labeled thymoquinone/curcumin liposome (Lip (TQ + CUR + ROD)) were prepared with encapsulation efficiency exceeding 99%. The aim of the present study was to evaluate the effect of the different prepared formulations either labeled with the fluorescent dye (rhodamine B) or not on A549 lung cancer cells. Cytotoxicity of different formulations was assessed by MTT assay. Proliferation of A549 cells was significantly inhibited by the different formulations in a concentration-dependent manner in 72 h. The Lip (TQ + CUR + ROD) formulation demonstrated the lowest IC
50
value. To investigate its mechanism of action on A549 lung cancer cells, the Comet assay (for DNA damage) was done, the measurement of some oxidative stress parameters in addition to performing inverted fluorescence microscopy imaging. The results of the present study demonstrated the increased DNA damage, oxidative stress damage, and cell apoptosis in A549 treated with TQ, CUR, and rhodamine-encapsulated fluorescent liposome formulation as compared to untreated cells. The results obtained from the present study demonstrate the significant role of the TQ/CUR fluorescent liposomes on decreasing the viability of A549 lung cancer cells.
Graphical abstract</description><subject>Apoptosis</subject><subject>Biomedical and Life Sciences</subject><subject>Biomedicine</subject><subject>Cell proliferation</subject><subject>Comet assay</subject><subject>Curcumin</subject><subject>Cytotoxicity</subject><subject>Deoxyribonucleic acid</subject><subject>DNA</subject><subject>DNA damage</subject><subject>Encapsulation</subject><subject>Fluorescence</subject><subject>Fluorescence microscopy</subject><subject>Fluorescent dyes</subject><subject>Fluorescent indicators</subject><subject>Formulations</subject><subject>Liposomes</subject><subject>Lung cancer</subject><subject>Neurosciences</subject><subject>Original Article</subject><subject>Oxidative stress</subject><subject>Pharmacology/Toxicology</subject><subject>Rhodamine</subject><subject>Viability</subject><issn>0028-1298</issn><issn>1432-1912</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2019</creationdate><recordtype>article</recordtype><sourceid>BENPR</sourceid><recordid>eNp9kE9LAzEQxYMotla_gAdZ8OJlbSbpZpOjFP8UCiLoOWyzWU3Z3dQkK-63N-1WBQ8ehoGZ37y8PITOAV8DxvnUY0wApxhELMZ5CgdoDDNKUhBADtE47uOQCD5CJ96vMcYMsuwYjSjQPOecjNHTok0-THA28aEr-8RWSXjTieqDDfbTKBP2s76x751pbaunqnOqa0ybVHVnnfZKtyGpzcZ622h_io6qovb6bN8n6OXu9nn-kC4f7xfzm2WqaJ6FFHKWFVSQXABQqoTmumSiwgXlbMV3P5sxrHRZ0hXJclyCygpFKzabKSEqTifoatDduOhM-yAbE63UddFq23lJCONZnm3VJ-jyD7q2nWujux0FmBNGIkUGSjnrvdOV3DjTFK6XgOU2cDkELmPgche4hHh0sZfuVo0uf06-E44AHQAfV-2rdr9v_yP7BZEQiwo</recordid><startdate>20191101</startdate><enddate>20191101</enddate><creator>Fahmy, Heba Mohamed</creator><general>Springer Berlin Heidelberg</general><general>Springer Nature B.V</general><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7QP</scope><scope>7TK</scope><scope>7X7</scope><scope>7XB</scope><scope>88E</scope><scope>8AO</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>BENPR</scope><scope>CCPQU</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>K9.</scope><scope>M0S</scope><scope>M1P</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>7X8</scope><orcidid>https://orcid.org/0000-0001-8689-1198</orcidid></search><sort><creationdate>20191101</creationdate><title>In vitro study of the cytotoxicity of thymoquinone/curcumin fluorescent liposomes</title><author>Fahmy, Heba Mohamed</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c375t-1765a392791133c9e8ed69f0a386b800210460cedd3b2570d1c5ac3f644c99f83</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2019</creationdate><topic>Apoptosis</topic><topic>Biomedical and Life Sciences</topic><topic>Biomedicine</topic><topic>Cell proliferation</topic><topic>Comet assay</topic><topic>Curcumin</topic><topic>Cytotoxicity</topic><topic>Deoxyribonucleic acid</topic><topic>DNA</topic><topic>DNA damage</topic><topic>Encapsulation</topic><topic>Fluorescence</topic><topic>Fluorescence microscopy</topic><topic>Fluorescent dyes</topic><topic>Fluorescent indicators</topic><topic>Formulations</topic><topic>Liposomes</topic><topic>Lung cancer</topic><topic>Neurosciences</topic><topic>Original Article</topic><topic>Oxidative stress</topic><topic>Pharmacology/Toxicology</topic><topic>Rhodamine</topic><topic>Viability</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Fahmy, Heba Mohamed</creatorcontrib><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Calcium & Calcified Tissue Abstracts</collection><collection>Neurosciences Abstracts</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Medical Database (Alumni Edition)</collection><collection>ProQuest Pharma Collection</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>ProQuest Central (Alumni)</collection><collection>ProQuest Central</collection><collection>ProQuest Central</collection><collection>ProQuest One Community College</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Medical Database</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central China</collection><collection>MEDLINE - Academic</collection><jtitle>Naunyn-Schmiedeberg's archives of pharmacology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Fahmy, Heba Mohamed</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>In vitro study of the cytotoxicity of thymoquinone/curcumin fluorescent liposomes</atitle><jtitle>Naunyn-Schmiedeberg's archives of pharmacology</jtitle><stitle>Naunyn-Schmiedeberg's Arch Pharmacol</stitle><addtitle>Naunyn Schmiedebergs Arch Pharmacol</addtitle><date>2019-11-01</date><risdate>2019</risdate><volume>392</volume><issue>11</issue><spage>1465</spage><epage>1476</epage><pages>1465-1476</pages><issn>0028-1298</issn><eissn>1432-1912</eissn><abstract>In the present study, thymoquinone-loaded liposomes (Lip (TQ)), curcumin-encapsulated liposome (Lip (CUR)), and thymoquinone/curcumin-encapsulated liposome (Lip (TQ + CUR)) in addition to rhodamine-labeled thymoquinone/curcumin liposome (Lip (TQ + CUR + ROD)) were prepared with encapsulation efficiency exceeding 99%. The aim of the present study was to evaluate the effect of the different prepared formulations either labeled with the fluorescent dye (rhodamine B) or not on A549 lung cancer cells. Cytotoxicity of different formulations was assessed by MTT assay. Proliferation of A549 cells was significantly inhibited by the different formulations in a concentration-dependent manner in 72 h. The Lip (TQ + CUR + ROD) formulation demonstrated the lowest IC
50
value. To investigate its mechanism of action on A549 lung cancer cells, the Comet assay (for DNA damage) was done, the measurement of some oxidative stress parameters in addition to performing inverted fluorescence microscopy imaging. The results of the present study demonstrated the increased DNA damage, oxidative stress damage, and cell apoptosis in A549 treated with TQ, CUR, and rhodamine-encapsulated fluorescent liposome formulation as compared to untreated cells. The results obtained from the present study demonstrate the significant role of the TQ/CUR fluorescent liposomes on decreasing the viability of A549 lung cancer cells.
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| subjects | Apoptosis Biomedical and Life Sciences Biomedicine Cell proliferation Comet assay Curcumin Cytotoxicity Deoxyribonucleic acid DNA DNA damage Encapsulation Fluorescence Fluorescence microscopy Fluorescent dyes Fluorescent indicators Formulations Liposomes Lung cancer Neurosciences Original Article Oxidative stress Pharmacology/Toxicology Rhodamine Viability |
| title | In vitro study of the cytotoxicity of thymoquinone/curcumin fluorescent liposomes |
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