An Automated and Qualified Platform Method for Site-Specific Succinimide and Deamidation Quantitation Using Low-pH Peptide Mapping

mAbs undergo several post-translational modifications, including the formation of succinimide from the deamidation of asparagine or the isomerization of aspartic acid. Because of the potential impact of succinimide formation on the biological activity of mAbs, detection and quantification of this sp...

Ausführliche Beschreibung

Bibliographische Detailangaben

Veröffentlicht in:	Journal of pharmaceutical sciences 2019-11, Vol.108 (11), p.3540-3549
Hauptverfasser:	Cao, Mingyan, Xu, Weichen, Niu, Ben, Kabundi, Ivy, Luo, Haibin, Prophet, Meagan, Chen, Weimin, Liu, Dengfeng, Saveliev, Sergei V., Urh, Marjeta, Wang, Jihong
Format:	Artikel
Sprache:	eng
Schlagworte:	analytical chemistry automation biopharmaceutical characterization deamidation liquid chromatography-mass spectrometry (LC-MS) mAb(s) physicochemical properties
Online-Zugang:	Volltext
Tags:	Tag hinzufügen Keine Tags, Fügen Sie den ersten Tag hinzu!