A seminested RT-PCR for molecular genotyping of the Brazilian BR-I Infectious Bronchitis Virus Strain (GI-11)
Infectious bronchitis (IB) is one of the avian diseases with the greatest impact on poultry farming worldwide. In Brazil, strain BR-I (GI-11) is the most prevalent in poultry flocks. The present study aimed to develop a seminested RT-PCR assay specific for the diagnosis of BR-I IBV in Brazilian samp...
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| Veröffentlicht in: | Molecular and cellular probes 2019-10, Vol.47, p.101426-101426, Article 101426 |
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| container_title | Molecular and cellular probes |
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| creator | Chacón, Ruy D. Astolfi-Ferreira, Claudete S. Chacón, Jorge L. Nuñez, Luis F.N. De la Torre, David I. Piantino Ferreira, Antonio J. |
| description | Infectious bronchitis (IB) is one of the avian diseases with the greatest impact on poultry farming worldwide. In Brazil, strain BR-I (GI-11) is the most prevalent in poultry flocks. The present study aimed to develop a seminested RT-PCR assay specific for the diagnosis of BR-I IBV in Brazilian samples, targeting subunit 1 of the S gene. The detection limit of this assay was 10 copies of the IBV genome. In this study, 62.24% of 572 organ pools from the 5 regions of Brazil tested positive in a 3′UTR screening, and 84.83% were typed as BR-I IBV. BR-I was detected in the respiratory, digestive and urogenital tracts in pooled samples from all Brazilian geographical regions and in all the breeding systems analyzed. Specificity and sensitivity tests as well as phylogenetic analysis successfully confirmed the expected clustering of the sequences detected by this assay with the BR-I (GI-11) group. The nested PCR described in this study represents a suitable and valuable tool in the diagnosis, epidemiology, monitoring and vaccination decisions of IBV.
•The seminested RT-PCR represents a useful tool in the diagnosis and epidemiology for monitoring IBV traits.•This IBV genotype GI-11 is highly disseminated in Latin America and its molecular characterization was presented in comparison with other genotypes.•The seminested RT-PCR represents a useful tool in the diagnosis, epidemiology, and monitoring of vaccination programs for IBV. |
| doi_str_mv | 10.1016/j.mcp.2019.101426 |
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•The seminested RT-PCR represents a useful tool in the diagnosis and epidemiology for monitoring IBV traits.•This IBV genotype GI-11 is highly disseminated in Latin America and its molecular characterization was presented in comparison with other genotypes.•The seminested RT-PCR represents a useful tool in the diagnosis, epidemiology, and monitoring of vaccination programs for IBV.</description><identifier>ISSN: 0890-8508</identifier><identifier>EISSN: 1096-1194</identifier><identifier>DOI: 10.1016/j.mcp.2019.101426</identifier><identifier>PMID: 31365883</identifier><language>eng</language><publisher>England: Elsevier Ltd</publisher><subject>3' Untranslated Regions ; Animals ; BR-I genotype ; Brazil ; Breeding ; Chicken ; Coronavirus Infections - diagnosis ; Coronavirus Infections - veterinary ; Detection ; Genotyping Techniques - veterinary ; GI-11 ; Infectious bronchitis virus ; Infectious bronchitis virus - classification ; Infectious bronchitis virus - genetics ; Infectious bronchitis virus - isolation & purification ; Limit of Detection ; Nested PCR ; Phylogeny ; Poultry ; Poultry Diseases - virology ; Reverse Transcriptase Polymerase Chain Reaction - veterinary ; Spike Glycoprotein, Coronavirus - genetics</subject><ispartof>Molecular and cellular probes, 2019-10, Vol.47, p.101426-101426, Article 101426</ispartof><rights>2019</rights><rights>Copyright © 2019. Published by Elsevier Ltd.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c353t-cf9ecb8a53536d4c876e00a7ba7f22c86fe6d117ad95531483ff8af9f6854d343</citedby><cites>FETCH-LOGICAL-c353t-cf9ecb8a53536d4c876e00a7ba7f22c86fe6d117ad95531483ff8af9f6854d343</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://www.sciencedirect.com/science/article/pii/S0890850819300854$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,776,780,3537,27901,27902,65306</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/31365883$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Chacón, Ruy D.</creatorcontrib><creatorcontrib>Astolfi-Ferreira, Claudete S.</creatorcontrib><creatorcontrib>Chacón, Jorge L.</creatorcontrib><creatorcontrib>Nuñez, Luis F.N.</creatorcontrib><creatorcontrib>De la Torre, David I.</creatorcontrib><creatorcontrib>Piantino Ferreira, Antonio J.</creatorcontrib><title>A seminested RT-PCR for molecular genotyping of the Brazilian BR-I Infectious Bronchitis Virus Strain (GI-11)</title><title>Molecular and cellular probes</title><addtitle>Mol Cell Probes</addtitle><description>Infectious bronchitis (IB) is one of the avian diseases with the greatest impact on poultry farming worldwide. In Brazil, strain BR-I (GI-11) is the most prevalent in poultry flocks. The present study aimed to develop a seminested RT-PCR assay specific for the diagnosis of BR-I IBV in Brazilian samples, targeting subunit 1 of the S gene. The detection limit of this assay was 10 copies of the IBV genome. In this study, 62.24% of 572 organ pools from the 5 regions of Brazil tested positive in a 3′UTR screening, and 84.83% were typed as BR-I IBV. BR-I was detected in the respiratory, digestive and urogenital tracts in pooled samples from all Brazilian geographical regions and in all the breeding systems analyzed. Specificity and sensitivity tests as well as phylogenetic analysis successfully confirmed the expected clustering of the sequences detected by this assay with the BR-I (GI-11) group. The nested PCR described in this study represents a suitable and valuable tool in the diagnosis, epidemiology, monitoring and vaccination decisions of IBV.
•The seminested RT-PCR represents a useful tool in the diagnosis and epidemiology for monitoring IBV traits.•This IBV genotype GI-11 is highly disseminated in Latin America and its molecular characterization was presented in comparison with other genotypes.•The seminested RT-PCR represents a useful tool in the diagnosis, epidemiology, and monitoring of vaccination programs for IBV.</description><subject>3' Untranslated Regions</subject><subject>Animals</subject><subject>BR-I genotype</subject><subject>Brazil</subject><subject>Breeding</subject><subject>Chicken</subject><subject>Coronavirus Infections - diagnosis</subject><subject>Coronavirus Infections - veterinary</subject><subject>Detection</subject><subject>Genotyping Techniques - veterinary</subject><subject>GI-11</subject><subject>Infectious bronchitis virus</subject><subject>Infectious bronchitis virus - classification</subject><subject>Infectious bronchitis virus - genetics</subject><subject>Infectious bronchitis virus - isolation & purification</subject><subject>Limit of Detection</subject><subject>Nested PCR</subject><subject>Phylogeny</subject><subject>Poultry</subject><subject>Poultry Diseases - virology</subject><subject>Reverse Transcriptase Polymerase Chain Reaction - veterinary</subject><subject>Spike Glycoprotein, Coronavirus - genetics</subject><issn>0890-8508</issn><issn>1096-1194</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2019</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9kMtOwzAQRS0EglL4ADbIS1ik2HHiOGJFKx6VkEDlsbVcZwyuErvYKRJ8Pa5aWLIazcydqzsHoRNKRpRQfrEYdXo5ygmt132R8x00oKTmGaV1sYsGRNQkEyURB-gwxgUhpC6I2EcHjDJeCsEGqLvCETrrIPbQ4Nlz9jiZYeMD7nwLetWqgN_A-f5rad0b9gb374DHQX3b1iqHx7NsiqfOgO6tX8W08U6_295G_GpDGjz1QVmHz26nKdT5Edozqo1wvK1D9HJz_Ty5y-4fbqeTq_tMs5L1mTY16LlQZep4U2hRcSBEVXNVmTzXghvgDaWVauqyZLQQzBihTG24KIuGFWyIzja-y-A_Vuk32dmooW2VgxRT5jmvKkFKQZKUbqQ6-BgDGLkMtlPhS1Ii15TlQibKck1Zbiinm9Ot_WreQfN38Ys1CS43AkhPfloIMmoLTkNjQ0IlG2__sf8B14KLwQ</recordid><startdate>201910</startdate><enddate>201910</enddate><creator>Chacón, Ruy D.</creator><creator>Astolfi-Ferreira, Claudete S.</creator><creator>Chacón, Jorge L.</creator><creator>Nuñez, Luis F.N.</creator><creator>De la Torre, David I.</creator><creator>Piantino Ferreira, Antonio J.</creator><general>Elsevier Ltd</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>201910</creationdate><title>A seminested RT-PCR for molecular genotyping of the Brazilian BR-I Infectious Bronchitis Virus Strain (GI-11)</title><author>Chacón, Ruy D. ; Astolfi-Ferreira, Claudete S. ; Chacón, Jorge L. ; Nuñez, Luis F.N. ; De la Torre, David I. ; Piantino Ferreira, Antonio J.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c353t-cf9ecb8a53536d4c876e00a7ba7f22c86fe6d117ad95531483ff8af9f6854d343</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2019</creationdate><topic>3' Untranslated Regions</topic><topic>Animals</topic><topic>BR-I genotype</topic><topic>Brazil</topic><topic>Breeding</topic><topic>Chicken</topic><topic>Coronavirus Infections - diagnosis</topic><topic>Coronavirus Infections - veterinary</topic><topic>Detection</topic><topic>Genotyping Techniques - veterinary</topic><topic>GI-11</topic><topic>Infectious bronchitis virus</topic><topic>Infectious bronchitis virus - classification</topic><topic>Infectious bronchitis virus - genetics</topic><topic>Infectious bronchitis virus - isolation & purification</topic><topic>Limit of Detection</topic><topic>Nested PCR</topic><topic>Phylogeny</topic><topic>Poultry</topic><topic>Poultry Diseases - virology</topic><topic>Reverse Transcriptase Polymerase Chain Reaction - veterinary</topic><topic>Spike Glycoprotein, Coronavirus - genetics</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Chacón, Ruy D.</creatorcontrib><creatorcontrib>Astolfi-Ferreira, Claudete S.</creatorcontrib><creatorcontrib>Chacón, Jorge L.</creatorcontrib><creatorcontrib>Nuñez, Luis F.N.</creatorcontrib><creatorcontrib>De la Torre, David I.</creatorcontrib><creatorcontrib>Piantino Ferreira, Antonio J.</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Molecular and cellular probes</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Chacón, Ruy D.</au><au>Astolfi-Ferreira, Claudete S.</au><au>Chacón, Jorge L.</au><au>Nuñez, Luis F.N.</au><au>De la Torre, David I.</au><au>Piantino Ferreira, Antonio J.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>A seminested RT-PCR for molecular genotyping of the Brazilian BR-I Infectious Bronchitis Virus Strain (GI-11)</atitle><jtitle>Molecular and cellular probes</jtitle><addtitle>Mol Cell Probes</addtitle><date>2019-10</date><risdate>2019</risdate><volume>47</volume><spage>101426</spage><epage>101426</epage><pages>101426-101426</pages><artnum>101426</artnum><issn>0890-8508</issn><eissn>1096-1194</eissn><abstract>Infectious bronchitis (IB) is one of the avian diseases with the greatest impact on poultry farming worldwide. In Brazil, strain BR-I (GI-11) is the most prevalent in poultry flocks. The present study aimed to develop a seminested RT-PCR assay specific for the diagnosis of BR-I IBV in Brazilian samples, targeting subunit 1 of the S gene. The detection limit of this assay was 10 copies of the IBV genome. In this study, 62.24% of 572 organ pools from the 5 regions of Brazil tested positive in a 3′UTR screening, and 84.83% were typed as BR-I IBV. BR-I was detected in the respiratory, digestive and urogenital tracts in pooled samples from all Brazilian geographical regions and in all the breeding systems analyzed. Specificity and sensitivity tests as well as phylogenetic analysis successfully confirmed the expected clustering of the sequences detected by this assay with the BR-I (GI-11) group. The nested PCR described in this study represents a suitable and valuable tool in the diagnosis, epidemiology, monitoring and vaccination decisions of IBV.
•The seminested RT-PCR represents a useful tool in the diagnosis and epidemiology for monitoring IBV traits.•This IBV genotype GI-11 is highly disseminated in Latin America and its molecular characterization was presented in comparison with other genotypes.•The seminested RT-PCR represents a useful tool in the diagnosis, epidemiology, and monitoring of vaccination programs for IBV.</abstract><cop>England</cop><pub>Elsevier Ltd</pub><pmid>31365883</pmid><doi>10.1016/j.mcp.2019.101426</doi><tpages>1</tpages></addata></record> |
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| subjects | 3' Untranslated Regions Animals BR-I genotype Brazil Breeding Chicken Coronavirus Infections - diagnosis Coronavirus Infections - veterinary Detection Genotyping Techniques - veterinary GI-11 Infectious bronchitis virus Infectious bronchitis virus - classification Infectious bronchitis virus - genetics Infectious bronchitis virus - isolation & purification Limit of Detection Nested PCR Phylogeny Poultry Poultry Diseases - virology Reverse Transcriptase Polymerase Chain Reaction - veterinary Spike Glycoprotein, Coronavirus - genetics |
| title | A seminested RT-PCR for molecular genotyping of the Brazilian BR-I Infectious Bronchitis Virus Strain (GI-11) |
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