Exploiting the diversity of streptococcal hyaluronan synthases for the production of molecular weight–tailored hyaluronan
The molecular weight (Mw) of hyaluronic acid (HA) determines its suitability for medical and cosmetic applications. Here, we characterize in vitro and in vivo HA synthesis of streptococcal HA synthases (HASs) with a special focus on HA Mw. To date, four streptococcal HA producers are described ( Str...
Gespeichert in:
| Veröffentlicht in: | Applied microbiology and biotechnology 2019-09, Vol.103 (18), p.7567-7581 |
|---|---|
| Hauptverfasser: | , , , , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 7581 |
|---|---|
| container_issue | 18 |
| container_start_page | 7567 |
| container_title | Applied microbiology and biotechnology |
| container_volume | 103 |
| creator | Schulte, Sandra Doss, Sreeja Shanmuga Jeeva, Pandeeswari Ananth, Mythili Blank, Lars M. Jayaraman, Guhan |
| description | The molecular weight (Mw) of hyaluronic acid (HA) determines its suitability for medical and cosmetic applications. Here, we characterize in vitro and in vivo HA synthesis of streptococcal HA synthases (HASs) with a special focus on HA Mw. To date, four streptococcal HA producers are described (
Streptococcus equi
subsp.
equi
,
S. equi
subsp.
zooepidemicus
,
S. pyogenes
, and
S. uberis
). We identified two more potential HA producers in this study:
S. iniae
and
S. parauberis
. Indeed, the HA Mw produced by the different streptococcal HASs differs in vitro. To exploit these different HA Mw synthesis capacities,
Lactococcus lactis
strains expressing the streptococcal HASs were constructed. HA of different Mw was also produced in vivo by these engineered strains, strongly suggesting that the protein sequences of the HASs influence HA Mw. Since the HA Mw in vivo is also influenced by metabolic factors like specific growth rate and HA precursor availability, these were also determined. In summary, the maximal Mw of HA synthesized is specific for the individual synthase, while any decrease from the maximal HA Mw is influenced by physiological and metabolic factors. The results open new avenues for Mw-tailored HA synthesis. |
| doi_str_mv | 10.1007/s00253-019-10023-w |
| format | Article |
| fullrecord | <record><control><sourceid>gale_proqu</sourceid><recordid>TN_cdi_proquest_miscellaneous_2267747107</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><galeid>A597980580</galeid><sourcerecordid>A597980580</sourcerecordid><originalsourceid>FETCH-LOGICAL-c513t-b0a441f420beaeb2c7e34bcd08ef660d98e0f1b584d29fa86385afd14b5ced373</originalsourceid><addsrcrecordid>eNp9ks1u1DAUhSMEokPhBVigSGxgkeKfJHaWVVWgUiUkftaW41xnXDn2YDtMR2z6Dn1DngTPTKEMQsgLy9ffObr36hTFc4xOMELsTUSINLRCuKvym9Bq_aBY4JqSCrW4flgsEGZNxZqOHxVPYrxCCBPeto-LI4ppy3jDFsX38-uV9SYZN5ZpCeVgvkGIJm1Kr8uYAqySV14pacvlRto5eCddGTcuLWWEWGofdrpV8MOskvFuK5y8BTVbGco1mHGZftzcJmmsDzD8YfO0eKSljfDs7j4uvrw9_3z2vrr88O7i7PSyUg2mqeqRrGusa4J6kNATxYDWvRoQB922aOg4II37htcD6bTkLeWN1AOu-0bBQBk9Ll7tfXOTX2eISUwmKrBWOvBzFIS0jNUMoy368i_0ys_B5e52FOF5md09NUoLwjjtU5BqaypOm451HDUcZerkH1Q-A0xGeQfa5PqB4PWBIDMJrtMo5xjFxaePhyzZsyr4GANosQpmkmEjMBLbdIh9OkROh9ilQ6yz6MXddHM_wfBb8isOGaB7IOYvN0K4H_8_tj8B7OfH4Q</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>2267285989</pqid></control><display><type>article</type><title>Exploiting the diversity of streptococcal hyaluronan synthases for the production of molecular weight–tailored hyaluronan</title><source>MEDLINE</source><source>SpringerLink Journals</source><creator>Schulte, Sandra ; Doss, Sreeja Shanmuga ; Jeeva, Pandeeswari ; Ananth, Mythili ; Blank, Lars M. ; Jayaraman, Guhan</creator><creatorcontrib>Schulte, Sandra ; Doss, Sreeja Shanmuga ; Jeeva, Pandeeswari ; Ananth, Mythili ; Blank, Lars M. ; Jayaraman, Guhan</creatorcontrib><description>The molecular weight (Mw) of hyaluronic acid (HA) determines its suitability for medical and cosmetic applications. Here, we characterize in vitro and in vivo HA synthesis of streptococcal HA synthases (HASs) with a special focus on HA Mw. To date, four streptococcal HA producers are described (
Streptococcus equi
subsp.
equi
,
S. equi
subsp.
zooepidemicus
,
S. pyogenes
, and
S. uberis
). We identified two more potential HA producers in this study:
S. iniae
and
S. parauberis
. Indeed, the HA Mw produced by the different streptococcal HASs differs in vitro. To exploit these different HA Mw synthesis capacities,
Lactococcus lactis
strains expressing the streptococcal HASs were constructed. HA of different Mw was also produced in vivo by these engineered strains, strongly suggesting that the protein sequences of the HASs influence HA Mw. Since the HA Mw in vivo is also influenced by metabolic factors like specific growth rate and HA precursor availability, these were also determined. In summary, the maximal Mw of HA synthesized is specific for the individual synthase, while any decrease from the maximal HA Mw is influenced by physiological and metabolic factors. The results open new avenues for Mw-tailored HA synthesis.</description><identifier>ISSN: 0175-7598</identifier><identifier>EISSN: 1432-0614</identifier><identifier>DOI: 10.1007/s00253-019-10023-w</identifier><identifier>PMID: 31367857</identifier><language>eng</language><publisher>Berlin/Heidelberg: Springer Berlin Heidelberg</publisher><subject>Bacterial Proteins - genetics ; Bacterial Proteins - metabolism ; Biomedical and Life Sciences ; Biotechnologically Relevant Enzymes and Proteins ; Biotechnology ; Growth rate ; Hyaluronan Synthases - genetics ; Hyaluronan Synthases - metabolism ; Hyaluronic acid ; Hyaluronic Acid - biosynthesis ; Lactococcus lactis ; Lactococcus lactis - genetics ; Lactococcus lactis - metabolism ; Life Sciences ; Metabolism ; Microbial Genetics and Genomics ; Microbiology ; Molecular Weight ; Physiological effects ; Streptococcus ; Streptococcus - enzymology ; Streptococcus - genetics ; Synthesis</subject><ispartof>Applied microbiology and biotechnology, 2019-09, Vol.103 (18), p.7567-7581</ispartof><rights>Springer-Verlag GmbH Germany, part of Springer Nature 2019</rights><rights>COPYRIGHT 2019 Springer</rights><rights>Applied Microbiology and Biotechnology is a copyright of Springer, (2019). All Rights Reserved.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c513t-b0a441f420beaeb2c7e34bcd08ef660d98e0f1b584d29fa86385afd14b5ced373</citedby><cites>FETCH-LOGICAL-c513t-b0a441f420beaeb2c7e34bcd08ef660d98e0f1b584d29fa86385afd14b5ced373</cites><orcidid>0000-0003-0961-4976</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://link.springer.com/content/pdf/10.1007/s00253-019-10023-w$$EPDF$$P50$$Gspringer$$H</linktopdf><linktohtml>$$Uhttps://link.springer.com/10.1007/s00253-019-10023-w$$EHTML$$P50$$Gspringer$$H</linktohtml><link.rule.ids>314,776,780,27901,27902,41464,42533,51294</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/31367857$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Schulte, Sandra</creatorcontrib><creatorcontrib>Doss, Sreeja Shanmuga</creatorcontrib><creatorcontrib>Jeeva, Pandeeswari</creatorcontrib><creatorcontrib>Ananth, Mythili</creatorcontrib><creatorcontrib>Blank, Lars M.</creatorcontrib><creatorcontrib>Jayaraman, Guhan</creatorcontrib><title>Exploiting the diversity of streptococcal hyaluronan synthases for the production of molecular weight–tailored hyaluronan</title><title>Applied microbiology and biotechnology</title><addtitle>Appl Microbiol Biotechnol</addtitle><addtitle>Appl Microbiol Biotechnol</addtitle><description>The molecular weight (Mw) of hyaluronic acid (HA) determines its suitability for medical and cosmetic applications. Here, we characterize in vitro and in vivo HA synthesis of streptococcal HA synthases (HASs) with a special focus on HA Mw. To date, four streptococcal HA producers are described (
Streptococcus equi
subsp.
equi
,
S. equi
subsp.
zooepidemicus
,
S. pyogenes
, and
S. uberis
). We identified two more potential HA producers in this study:
S. iniae
and
S. parauberis
. Indeed, the HA Mw produced by the different streptococcal HASs differs in vitro. To exploit these different HA Mw synthesis capacities,
Lactococcus lactis
strains expressing the streptococcal HASs were constructed. HA of different Mw was also produced in vivo by these engineered strains, strongly suggesting that the protein sequences of the HASs influence HA Mw. Since the HA Mw in vivo is also influenced by metabolic factors like specific growth rate and HA precursor availability, these were also determined. In summary, the maximal Mw of HA synthesized is specific for the individual synthase, while any decrease from the maximal HA Mw is influenced by physiological and metabolic factors. The results open new avenues for Mw-tailored HA synthesis.</description><subject>Bacterial Proteins - genetics</subject><subject>Bacterial Proteins - metabolism</subject><subject>Biomedical and Life Sciences</subject><subject>Biotechnologically Relevant Enzymes and Proteins</subject><subject>Biotechnology</subject><subject>Growth rate</subject><subject>Hyaluronan Synthases - genetics</subject><subject>Hyaluronan Synthases - metabolism</subject><subject>Hyaluronic acid</subject><subject>Hyaluronic Acid - biosynthesis</subject><subject>Lactococcus lactis</subject><subject>Lactococcus lactis - genetics</subject><subject>Lactococcus lactis - metabolism</subject><subject>Life Sciences</subject><subject>Metabolism</subject><subject>Microbial Genetics and Genomics</subject><subject>Microbiology</subject><subject>Molecular Weight</subject><subject>Physiological effects</subject><subject>Streptococcus</subject><subject>Streptococcus - enzymology</subject><subject>Streptococcus - genetics</subject><subject>Synthesis</subject><issn>0175-7598</issn><issn>1432-0614</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2019</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>BENPR</sourceid><recordid>eNp9ks1u1DAUhSMEokPhBVigSGxgkeKfJHaWVVWgUiUkftaW41xnXDn2YDtMR2z6Dn1DngTPTKEMQsgLy9ffObr36hTFc4xOMELsTUSINLRCuKvym9Bq_aBY4JqSCrW4flgsEGZNxZqOHxVPYrxCCBPeto-LI4ppy3jDFsX38-uV9SYZN5ZpCeVgvkGIJm1Kr8uYAqySV14pacvlRto5eCddGTcuLWWEWGofdrpV8MOskvFuK5y8BTVbGco1mHGZftzcJmmsDzD8YfO0eKSljfDs7j4uvrw9_3z2vrr88O7i7PSyUg2mqeqRrGusa4J6kNATxYDWvRoQB922aOg4II37htcD6bTkLeWN1AOu-0bBQBk9Ll7tfXOTX2eISUwmKrBWOvBzFIS0jNUMoy368i_0ys_B5e52FOF5md09NUoLwjjtU5BqaypOm451HDUcZerkH1Q-A0xGeQfa5PqB4PWBIDMJrtMo5xjFxaePhyzZsyr4GANosQpmkmEjMBLbdIh9OkROh9ilQ6yz6MXddHM_wfBb8isOGaB7IOYvN0K4H_8_tj8B7OfH4Q</recordid><startdate>20190901</startdate><enddate>20190901</enddate><creator>Schulte, Sandra</creator><creator>Doss, Sreeja Shanmuga</creator><creator>Jeeva, Pandeeswari</creator><creator>Ananth, Mythili</creator><creator>Blank, Lars M.</creator><creator>Jayaraman, Guhan</creator><general>Springer Berlin Heidelberg</general><general>Springer</general><general>Springer Nature B.V</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>ISR</scope><scope>3V.</scope><scope>7QL</scope><scope>7T7</scope><scope>7WY</scope><scope>7WZ</scope><scope>7X7</scope><scope>7XB</scope><scope>87Z</scope><scope>88A</scope><scope>88E</scope><scope>88I</scope><scope>8AO</scope><scope>8FD</scope><scope>8FE</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>8FL</scope><scope>ABUWG</scope><scope>AEUYN</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BEZIV</scope><scope>BHPHI</scope><scope>C1K</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FR3</scope><scope>FRNLG</scope><scope>FYUFA</scope><scope>F~G</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>HCIFZ</scope><scope>K60</scope><scope>K6~</scope><scope>K9.</scope><scope>L.-</scope><scope>LK8</scope><scope>M0C</scope><scope>M0S</scope><scope>M1P</scope><scope>M2P</scope><scope>M7N</scope><scope>M7P</scope><scope>P64</scope><scope>PQBIZ</scope><scope>PQBZA</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>Q9U</scope><scope>7X8</scope><orcidid>https://orcid.org/0000-0003-0961-4976</orcidid></search><sort><creationdate>20190901</creationdate><title>Exploiting the diversity of streptococcal hyaluronan synthases for the production of molecular weight–tailored hyaluronan</title><author>Schulte, Sandra ; Doss, Sreeja Shanmuga ; Jeeva, Pandeeswari ; Ananth, Mythili ; Blank, Lars M. ; Jayaraman, Guhan</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c513t-b0a441f420beaeb2c7e34bcd08ef660d98e0f1b584d29fa86385afd14b5ced373</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2019</creationdate><topic>Bacterial Proteins - genetics</topic><topic>Bacterial Proteins - metabolism</topic><topic>Biomedical and Life Sciences</topic><topic>Biotechnologically Relevant Enzymes and Proteins</topic><topic>Biotechnology</topic><topic>Growth rate</topic><topic>Hyaluronan Synthases - genetics</topic><topic>Hyaluronan Synthases - metabolism</topic><topic>Hyaluronic acid</topic><topic>Hyaluronic Acid - biosynthesis</topic><topic>Lactococcus lactis</topic><topic>Lactococcus lactis - genetics</topic><topic>Lactococcus lactis - metabolism</topic><topic>Life Sciences</topic><topic>Metabolism</topic><topic>Microbial Genetics and Genomics</topic><topic>Microbiology</topic><topic>Molecular Weight</topic><topic>Physiological effects</topic><topic>Streptococcus</topic><topic>Streptococcus - enzymology</topic><topic>Streptococcus - genetics</topic><topic>Synthesis</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Schulte, Sandra</creatorcontrib><creatorcontrib>Doss, Sreeja Shanmuga</creatorcontrib><creatorcontrib>Jeeva, Pandeeswari</creatorcontrib><creatorcontrib>Ananth, Mythili</creatorcontrib><creatorcontrib>Blank, Lars M.</creatorcontrib><creatorcontrib>Jayaraman, Guhan</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Gale In Context: Science</collection><collection>ProQuest Central (Corporate)</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Industrial and Applied Microbiology Abstracts (Microbiology A)</collection><collection>ABI/INFORM Collection</collection><collection>ABI/INFORM Global (PDF only)</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>ABI/INFORM Global (Alumni Edition)</collection><collection>Biology Database (Alumni Edition)</collection><collection>Medical Database (Alumni Edition)</collection><collection>Science Database (Alumni Edition)</collection><collection>ProQuest Pharma Collection</collection><collection>Technology Research Database</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>ABI/INFORM Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest One Sustainability</collection><collection>ProQuest Central UK/Ireland</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>ProQuest Central</collection><collection>Business Premium Collection</collection><collection>Natural Science Collection</collection><collection>Environmental Sciences and Pollution Management</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central Korea</collection><collection>Engineering Research Database</collection><collection>Business Premium Collection (Alumni)</collection><collection>Health Research Premium Collection</collection><collection>ABI/INFORM Global (Corporate)</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>SciTech Premium Collection</collection><collection>ProQuest Business Collection (Alumni Edition)</collection><collection>ProQuest Business Collection</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>ABI/INFORM Professional Advanced</collection><collection>ProQuest Biological Science Collection</collection><collection>ABI/INFORM Global</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Medical Database</collection><collection>Science Database</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>Biological Science Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>One Business (ProQuest)</collection><collection>ProQuest One Business (Alumni)</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central Basic</collection><collection>MEDLINE - Academic</collection><jtitle>Applied microbiology and biotechnology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Schulte, Sandra</au><au>Doss, Sreeja Shanmuga</au><au>Jeeva, Pandeeswari</au><au>Ananth, Mythili</au><au>Blank, Lars M.</au><au>Jayaraman, Guhan</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Exploiting the diversity of streptococcal hyaluronan synthases for the production of molecular weight–tailored hyaluronan</atitle><jtitle>Applied microbiology and biotechnology</jtitle><stitle>Appl Microbiol Biotechnol</stitle><addtitle>Appl Microbiol Biotechnol</addtitle><date>2019-09-01</date><risdate>2019</risdate><volume>103</volume><issue>18</issue><spage>7567</spage><epage>7581</epage><pages>7567-7581</pages><issn>0175-7598</issn><eissn>1432-0614</eissn><abstract>The molecular weight (Mw) of hyaluronic acid (HA) determines its suitability for medical and cosmetic applications. Here, we characterize in vitro and in vivo HA synthesis of streptococcal HA synthases (HASs) with a special focus on HA Mw. To date, four streptococcal HA producers are described (
Streptococcus equi
subsp.
equi
,
S. equi
subsp.
zooepidemicus
,
S. pyogenes
, and
S. uberis
). We identified two more potential HA producers in this study:
S. iniae
and
S. parauberis
. Indeed, the HA Mw produced by the different streptococcal HASs differs in vitro. To exploit these different HA Mw synthesis capacities,
Lactococcus lactis
strains expressing the streptococcal HASs were constructed. HA of different Mw was also produced in vivo by these engineered strains, strongly suggesting that the protein sequences of the HASs influence HA Mw. Since the HA Mw in vivo is also influenced by metabolic factors like specific growth rate and HA precursor availability, these were also determined. In summary, the maximal Mw of HA synthesized is specific for the individual synthase, while any decrease from the maximal HA Mw is influenced by physiological and metabolic factors. The results open new avenues for Mw-tailored HA synthesis.</abstract><cop>Berlin/Heidelberg</cop><pub>Springer Berlin Heidelberg</pub><pmid>31367857</pmid><doi>10.1007/s00253-019-10023-w</doi><tpages>15</tpages><orcidid>https://orcid.org/0000-0003-0961-4976</orcidid></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0175-7598 |
| ispartof | Applied microbiology and biotechnology, 2019-09, Vol.103 (18), p.7567-7581 |
| issn | 0175-7598 1432-0614 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_2267747107 |
| source | MEDLINE; SpringerLink Journals |
| subjects | Bacterial Proteins - genetics Bacterial Proteins - metabolism Biomedical and Life Sciences Biotechnologically Relevant Enzymes and Proteins Biotechnology Growth rate Hyaluronan Synthases - genetics Hyaluronan Synthases - metabolism Hyaluronic acid Hyaluronic Acid - biosynthesis Lactococcus lactis Lactococcus lactis - genetics Lactococcus lactis - metabolism Life Sciences Metabolism Microbial Genetics and Genomics Microbiology Molecular Weight Physiological effects Streptococcus Streptococcus - enzymology Streptococcus - genetics Synthesis |
| title | Exploiting the diversity of streptococcal hyaluronan synthases for the production of molecular weight–tailored hyaluronan |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-02-14T06%3A08%3A43IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-gale_proqu&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Exploiting%20the%20diversity%20of%20streptococcal%20hyaluronan%20synthases%20for%20the%20production%20of%20molecular%20weight%E2%80%93tailored%20hyaluronan&rft.jtitle=Applied%20microbiology%20and%20biotechnology&rft.au=Schulte,%20Sandra&rft.date=2019-09-01&rft.volume=103&rft.issue=18&rft.spage=7567&rft.epage=7581&rft.pages=7567-7581&rft.issn=0175-7598&rft.eissn=1432-0614&rft_id=info:doi/10.1007/s00253-019-10023-w&rft_dat=%3Cgale_proqu%3EA597980580%3C/gale_proqu%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=2267285989&rft_id=info:pmid/31367857&rft_galeid=A597980580&rfr_iscdi=true |