Calcium homeostasis in cisplatin resistant epithelial ovarian cancer

Calcium homeostasis in cisplatin resistant epithelial ovarian cancer

Intracellular calcium concentration ([Ca2+]i) may have an important role in the development of chemoresistance, which is an essential problem in cancer chemotherapy. Cisplatin (DDP), which modulates the intracellular calcium concentration by different mechanisms, is an antineoplastic agent with high...

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Veröffentlicht in:General physiology and biophysics 2019-01, Vol.38 (4), p.353-363
Hauptverfasser: Kucukkaya, Bahire, Basoglu, Harun, Erdag, Demet, Akbas, Fahri, Susgun, Seda, Yalcintepe, Leman
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container_start_page 353
container_title General physiology and biophysics
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creator Kucukkaya, Bahire
Basoglu, Harun
Erdag, Demet
Akbas, Fahri
Susgun, Seda
Yalcintepe, Leman
description Intracellular calcium concentration ([Ca2+]i) may have an important role in the development of chemoresistance, which is an essential problem in cancer chemotherapy. Cisplatin (DDP), which modulates the intracellular calcium concentration by different mechanisms, is an antineoplastic agent with high success rate in cancer therapies. We investigated the regulatory role of [Ca2+] in cisplatin resistance in epithelial ovarian cancer cell line, in MDAH-2774, and its chemoresistant subclone MDAH-2774/DDP. The measurement of [Ca2+]i using fluorescence microscope, and flow cytometry revealed that the amount of intracellular calcium decreased in cisplatin resistant cells compared to the amounts in parental cells. mRNA expression profiles of calcium homeostasis-associated major genes (IP3R1/2/3, RYR1/2, SERCA1/2/3, NCX1/2/3, PMCA1/2/3, and PMCA4) decreased in cisplatin resistant cell line in comparison to the expression profiles in parental cells. Owing to the changes in the expression of genes involved in calcium regulation, these results show, drug resistance may be prevented by introducing a new perspective on the use of inhibitors and activators of these genes, and thus of cytostatic treatment strategies, due to changes in the expression of genes involved in calcium regulation.
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title Calcium homeostasis in cisplatin resistant epithelial ovarian cancer
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