PLD1 promotes dendritic spine morphogenesis via activating PKD1
Dendritic spines on the dendrites of pyramidal neurons are one of the most important components for excitatory synapses, where excitatory information exchanges and integrates. The defects of dendritic spine development have been closely connected with many nervous system diseases including autism, i...
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| Veröffentlicht in: | Molecular and cellular neuroscience 2019-09, Vol.99, p.103394-103394, Article 103394 |
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| container_title | Molecular and cellular neuroscience |
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| creator | Li, Wen-Qi Luo, Li-Da Hu, Zhi-Wen Lyu, Tian-Jie Cen, Cheng Wang, Yun |
| description | Dendritic spines on the dendrites of pyramidal neurons are one of the most important components for excitatory synapses, where excitatory information exchanges and integrates. The defects of dendritic spine development have been closely connected with many nervous system diseases including autism, intellectual disability and so forth. Based on our previous studies, we here report a new functional signaling link between phospholipase D1 (PLD1) and protein kinase D1 (PKD1) in dendritic spine morphogenesis. Coimmunoprecipitation assays showed that PLD1 associates with PKD1. A series of knocking down and rescuing experiments demonstrated that PLD1 acts upstream of PKD1 in positively regulating dendritic spine morphogenesis. Using PLD1 inhibitor, we found that PLD1 activates PKD1 to promote dendritic spine morphogenesis. Thus, we further reveal the roles of the two different enzymes in neuronal development.
•PLD1 directly interacts with PKD1.•PLD1 functions upstream of PKD1 to promote dendritic spine development.•The enzymatic activity of PLD1 is required to promote dendritic spine development. |
| doi_str_mv | 10.1016/j.mcn.2019.103394 |
| format | Article |
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•PLD1 directly interacts with PKD1.•PLD1 functions upstream of PKD1 to promote dendritic spine development.•The enzymatic activity of PLD1 is required to promote dendritic spine development.</description><identifier>ISSN: 1044-7431</identifier><identifier>EISSN: 1095-9327</identifier><identifier>DOI: 10.1016/j.mcn.2019.103394</identifier><identifier>PMID: 31356881</identifier><language>eng</language><publisher>United States: Elsevier Inc</publisher><subject>Animals ; Cell Line ; Cells, Cultured ; Dendritic spine morphogenesis ; Dendritic Spines - metabolism ; Dendritic Spines - physiology ; Mice ; Neurogenesis ; Phospholipase D - antagonists & inhibitors ; Phospholipase D - metabolism ; Phospholipase D1 (PLD1) ; Protein Binding ; Protein kinase D1 (PKD1) ; Rats ; Rats, Sprague-Dawley ; TRPP Cation Channels - metabolism</subject><ispartof>Molecular and cellular neuroscience, 2019-09, Vol.99, p.103394-103394, Article 103394</ispartof><rights>2019 Elsevier Inc.</rights><rights>Copyright © 2019 Elsevier Inc. All rights reserved.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c353t-c6b559079096e425cebb3c97bb2be64f247f947a8db9dc1a6ae8f7a186f80e2d3</citedby><cites>FETCH-LOGICAL-c353t-c6b559079096e425cebb3c97bb2be64f247f947a8db9dc1a6ae8f7a186f80e2d3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://www.sciencedirect.com/science/article/pii/S1044743119300326$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,776,780,3537,27901,27902,65306</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/31356881$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Li, Wen-Qi</creatorcontrib><creatorcontrib>Luo, Li-Da</creatorcontrib><creatorcontrib>Hu, Zhi-Wen</creatorcontrib><creatorcontrib>Lyu, Tian-Jie</creatorcontrib><creatorcontrib>Cen, Cheng</creatorcontrib><creatorcontrib>Wang, Yun</creatorcontrib><title>PLD1 promotes dendritic spine morphogenesis via activating PKD1</title><title>Molecular and cellular neuroscience</title><addtitle>Mol Cell Neurosci</addtitle><description>Dendritic spines on the dendrites of pyramidal neurons are one of the most important components for excitatory synapses, where excitatory information exchanges and integrates. The defects of dendritic spine development have been closely connected with many nervous system diseases including autism, intellectual disability and so forth. Based on our previous studies, we here report a new functional signaling link between phospholipase D1 (PLD1) and protein kinase D1 (PKD1) in dendritic spine morphogenesis. Coimmunoprecipitation assays showed that PLD1 associates with PKD1. A series of knocking down and rescuing experiments demonstrated that PLD1 acts upstream of PKD1 in positively regulating dendritic spine morphogenesis. Using PLD1 inhibitor, we found that PLD1 activates PKD1 to promote dendritic spine morphogenesis. Thus, we further reveal the roles of the two different enzymes in neuronal development.
•PLD1 directly interacts with PKD1.•PLD1 functions upstream of PKD1 to promote dendritic spine development.•The enzymatic activity of PLD1 is required to promote dendritic spine development.</description><subject>Animals</subject><subject>Cell Line</subject><subject>Cells, Cultured</subject><subject>Dendritic spine morphogenesis</subject><subject>Dendritic Spines - metabolism</subject><subject>Dendritic Spines - physiology</subject><subject>Mice</subject><subject>Neurogenesis</subject><subject>Phospholipase D - antagonists & inhibitors</subject><subject>Phospholipase D - metabolism</subject><subject>Phospholipase D1 (PLD1)</subject><subject>Protein Binding</subject><subject>Protein kinase D1 (PKD1)</subject><subject>Rats</subject><subject>Rats, Sprague-Dawley</subject><subject>TRPP Cation Channels - metabolism</subject><issn>1044-7431</issn><issn>1095-9327</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2019</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9kMtOwzAQRS0EoqXwAWxQlmxS_IjtWCwQanmJSnQBa8t2JsVV88BOK_H3pEphyWpmpDNXMwehS4KnBBNxs55Wrp5STFQ_M6ayIzQmWPFUMSqP932WpTJjZITOYlxjjDlV7BSNGGFc5DkZo7vlYk6SNjRV00FMCqiL4Dvvktj6GpKqCe1ns4Iaoo_JzpvEuM7vTOfrVbJ8nZNzdFKaTYSLQ52gj8eH99lzunh7epndL1LHOOtSJyznCkuFlYCMcgfWMqektdSCyEqayVJl0uSFVYUjRhjIS2lILsocAy3YBF0Puf2pX1uIna58dLDZmBqabdSUCokJ5yzvUTKgLjQxBih1G3xlwrcmWO-96bXuvem9Nz1463euDvFbW0Hxt_ErqgduBwD6J3cego7OQ-2g8AFcp4vG_xP_A57VfQU</recordid><startdate>201909</startdate><enddate>201909</enddate><creator>Li, Wen-Qi</creator><creator>Luo, Li-Da</creator><creator>Hu, Zhi-Wen</creator><creator>Lyu, Tian-Jie</creator><creator>Cen, Cheng</creator><creator>Wang, Yun</creator><general>Elsevier Inc</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>201909</creationdate><title>PLD1 promotes dendritic spine morphogenesis via activating PKD1</title><author>Li, Wen-Qi ; Luo, Li-Da ; Hu, Zhi-Wen ; Lyu, Tian-Jie ; Cen, Cheng ; Wang, Yun</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c353t-c6b559079096e425cebb3c97bb2be64f247f947a8db9dc1a6ae8f7a186f80e2d3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2019</creationdate><topic>Animals</topic><topic>Cell Line</topic><topic>Cells, Cultured</topic><topic>Dendritic spine morphogenesis</topic><topic>Dendritic Spines - metabolism</topic><topic>Dendritic Spines - physiology</topic><topic>Mice</topic><topic>Neurogenesis</topic><topic>Phospholipase D - antagonists & inhibitors</topic><topic>Phospholipase D - metabolism</topic><topic>Phospholipase D1 (PLD1)</topic><topic>Protein Binding</topic><topic>Protein kinase D1 (PKD1)</topic><topic>Rats</topic><topic>Rats, Sprague-Dawley</topic><topic>TRPP Cation Channels - metabolism</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Li, Wen-Qi</creatorcontrib><creatorcontrib>Luo, Li-Da</creatorcontrib><creatorcontrib>Hu, Zhi-Wen</creatorcontrib><creatorcontrib>Lyu, Tian-Jie</creatorcontrib><creatorcontrib>Cen, Cheng</creatorcontrib><creatorcontrib>Wang, Yun</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Molecular and cellular neuroscience</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Li, Wen-Qi</au><au>Luo, Li-Da</au><au>Hu, Zhi-Wen</au><au>Lyu, Tian-Jie</au><au>Cen, Cheng</au><au>Wang, Yun</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>PLD1 promotes dendritic spine morphogenesis via activating PKD1</atitle><jtitle>Molecular and cellular neuroscience</jtitle><addtitle>Mol Cell Neurosci</addtitle><date>2019-09</date><risdate>2019</risdate><volume>99</volume><spage>103394</spage><epage>103394</epage><pages>103394-103394</pages><artnum>103394</artnum><issn>1044-7431</issn><eissn>1095-9327</eissn><abstract>Dendritic spines on the dendrites of pyramidal neurons are one of the most important components for excitatory synapses, where excitatory information exchanges and integrates. The defects of dendritic spine development have been closely connected with many nervous system diseases including autism, intellectual disability and so forth. Based on our previous studies, we here report a new functional signaling link between phospholipase D1 (PLD1) and protein kinase D1 (PKD1) in dendritic spine morphogenesis. Coimmunoprecipitation assays showed that PLD1 associates with PKD1. A series of knocking down and rescuing experiments demonstrated that PLD1 acts upstream of PKD1 in positively regulating dendritic spine morphogenesis. Using PLD1 inhibitor, we found that PLD1 activates PKD1 to promote dendritic spine morphogenesis. Thus, we further reveal the roles of the two different enzymes in neuronal development.
•PLD1 directly interacts with PKD1.•PLD1 functions upstream of PKD1 to promote dendritic spine development.•The enzymatic activity of PLD1 is required to promote dendritic spine development.</abstract><cop>United States</cop><pub>Elsevier Inc</pub><pmid>31356881</pmid><doi>10.1016/j.mcn.2019.103394</doi><tpages>1</tpages></addata></record> |
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| ispartof | Molecular and cellular neuroscience, 2019-09, Vol.99, p.103394-103394, Article 103394 |
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| subjects | Animals Cell Line Cells, Cultured Dendritic spine morphogenesis Dendritic Spines - metabolism Dendritic Spines - physiology Mice Neurogenesis Phospholipase D - antagonists & inhibitors Phospholipase D - metabolism Phospholipase D1 (PLD1) Protein Binding Protein kinase D1 (PKD1) Rats Rats, Sprague-Dawley TRPP Cation Channels - metabolism |
| title | PLD1 promotes dendritic spine morphogenesis via activating PKD1 |
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