Noncovalent tethering of nucleic acid aptamer on DNA nanostructure for targeted photo/chemo/gene therapies
Here, we report various therapeutic cargo-loadable DNA nanostructures that are shelled in polydopamine and noncovalently tethered with cancer cell-targeting DNA aptamers. Initial DNA nanostructure was formed by rolling-circle amplification and condensation with Mu peptides. This DNA nanostructure wa...
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| Veröffentlicht in: | Nanomedicine 2020-02, Vol.24, p.102053-102053, Article 102053 |
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| creator | Shim, Gayong Park, Jinwon Kim, Mi-Gyeong Yang, Geon Lee, Yeon Oh, Yu-Kyoung |
| description | Here, we report various therapeutic cargo-loadable DNA nanostructures that are shelled in polydopamine and noncovalently tethered with cancer cell-targeting DNA aptamers. Initial DNA nanostructure was formed by rolling-circle amplification and condensation with Mu peptides. This DNA nanostructure was loaded with an antisense oligonucleotide, a photosensitizer, or an anticancer chemotherapeutic drug. Each therapeutic agent-loaded DNA nanostructure was then shelled with polydopamine (PDA), and noncovalently decorated with a poly adenine-tailed nucleic acid aptamer (PA) specific for PTK7 receptor, resulting in PA-tethered and PDA-shelled DNA nanostructure (PA/PDN). PDA coating shell enabled photothermal therapy. In the cells overexpressing PTK7 receptor, photosensitizer-loaded PA/PDN showed greater photodynamic activity. Doxorubicin-loaded PA/PDN exerted higher anticancer activity than the other groups. Antisense oligonucleotide-loaded PA/PDN provided selective reduction of target proteins compared with other groups. Our results suggest that the PA-tethered and PDA-shelled DNA nanostructures could enable the specific receptor-targeted phototherapy, chemotherapy, and gene therapy against cancer cells.
DNA nanostructures with various cargoes were shelled with polydopamine and noncovalently tethered with nucleic acid aptamer. The nanostructures enable specific receptor-targeted photo/chemo and gene therapies. [Display omitted] |
| doi_str_mv | 10.1016/j.nano.2019.102053 |
| format | Article |
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DNA nanostructures with various cargoes were shelled with polydopamine and noncovalently tethered with nucleic acid aptamer. The nanostructures enable specific receptor-targeted photo/chemo and gene therapies. [Display omitted]</description><identifier>ISSN: 1549-9634</identifier><identifier>EISSN: 1549-9642</identifier><identifier>DOI: 10.1016/j.nano.2019.102053</identifier><identifier>PMID: 31344502</identifier><language>eng</language><publisher>United States: Elsevier Inc</publisher><subject>DNA nanostructure ; Noncovalent DNA aptamer anchoring ; Polydopamine shell ; Targeted therapy ; Versatile cargoes</subject><ispartof>Nanomedicine, 2020-02, Vol.24, p.102053-102053, Article 102053</ispartof><rights>2019 Elsevier Inc.</rights><rights>Copyright © 2019 Elsevier Inc. All rights reserved.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c356t-a5cccddee085ef8aeec60ee3bc51d64e1d8b4a779956b3f6c6262ece343a90103</citedby><cites>FETCH-LOGICAL-c356t-a5cccddee085ef8aeec60ee3bc51d64e1d8b4a779956b3f6c6262ece343a90103</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/j.nano.2019.102053$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,780,784,3550,27924,27925,45995</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/31344502$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Shim, Gayong</creatorcontrib><creatorcontrib>Park, Jinwon</creatorcontrib><creatorcontrib>Kim, Mi-Gyeong</creatorcontrib><creatorcontrib>Yang, Geon</creatorcontrib><creatorcontrib>Lee, Yeon</creatorcontrib><creatorcontrib>Oh, Yu-Kyoung</creatorcontrib><title>Noncovalent tethering of nucleic acid aptamer on DNA nanostructure for targeted photo/chemo/gene therapies</title><title>Nanomedicine</title><addtitle>Nanomedicine</addtitle><description>Here, we report various therapeutic cargo-loadable DNA nanostructures that are shelled in polydopamine and noncovalently tethered with cancer cell-targeting DNA aptamers. Initial DNA nanostructure was formed by rolling-circle amplification and condensation with Mu peptides. This DNA nanostructure was loaded with an antisense oligonucleotide, a photosensitizer, or an anticancer chemotherapeutic drug. Each therapeutic agent-loaded DNA nanostructure was then shelled with polydopamine (PDA), and noncovalently decorated with a poly adenine-tailed nucleic acid aptamer (PA) specific for PTK7 receptor, resulting in PA-tethered and PDA-shelled DNA nanostructure (PA/PDN). PDA coating shell enabled photothermal therapy. In the cells overexpressing PTK7 receptor, photosensitizer-loaded PA/PDN showed greater photodynamic activity. Doxorubicin-loaded PA/PDN exerted higher anticancer activity than the other groups. Antisense oligonucleotide-loaded PA/PDN provided selective reduction of target proteins compared with other groups. Our results suggest that the PA-tethered and PDA-shelled DNA nanostructures could enable the specific receptor-targeted phototherapy, chemotherapy, and gene therapy against cancer cells.
DNA nanostructures with various cargoes were shelled with polydopamine and noncovalently tethered with nucleic acid aptamer. The nanostructures enable specific receptor-targeted photo/chemo and gene therapies. [Display omitted]</description><subject>DNA nanostructure</subject><subject>Noncovalent DNA aptamer anchoring</subject><subject>Polydopamine shell</subject><subject>Targeted therapy</subject><subject>Versatile cargoes</subject><issn>1549-9634</issn><issn>1549-9642</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2020</creationdate><recordtype>article</recordtype><recordid>eNp9kEtv2zAQhImgQV7tH-ih4LEX23zLAnoJkjYtECSX9kzQy5VNQyJVkgrQf18JTnPsaReLmVnMR8hHztaccbM5rqOLaS0Yb-eDYFqekSuuVbtqjRLv3napLsl1KUfGZMNYe0EuJZdKaSauyPEpRUgvrsdYacV6wBzinqaOxgl6DEAdBE_dWN2AmaZI759u6fK31DxBnTLSLmVaXd5jRU_HQ6ppAwcc0maPEekS6caA5T0571xf8MPrvCG_vn39efd99fj88OPu9nEFUpu6choAvEdkW43d1iGCYYhyB5p7o5D77U65pmlbbXayM2CEEQgolXQt40zekM-n3DGn3xOWaodQAPveRUxTsUIY3TTSqHaWipMUciolY2fHHAaX_1jO7MLYHu3S1S6M7YnxbPr0mj_tBvRvln9QZ8GXkwDnli8Bsy0QMAL6kBGq9Sn8L_8vZfCPhw</recordid><startdate>202002</startdate><enddate>202002</enddate><creator>Shim, Gayong</creator><creator>Park, Jinwon</creator><creator>Kim, Mi-Gyeong</creator><creator>Yang, Geon</creator><creator>Lee, Yeon</creator><creator>Oh, Yu-Kyoung</creator><general>Elsevier Inc</general><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>202002</creationdate><title>Noncovalent tethering of nucleic acid aptamer on DNA nanostructure for targeted photo/chemo/gene therapies</title><author>Shim, Gayong ; Park, Jinwon ; Kim, Mi-Gyeong ; Yang, Geon ; Lee, Yeon ; Oh, Yu-Kyoung</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c356t-a5cccddee085ef8aeec60ee3bc51d64e1d8b4a779956b3f6c6262ece343a90103</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2020</creationdate><topic>DNA nanostructure</topic><topic>Noncovalent DNA aptamer anchoring</topic><topic>Polydopamine shell</topic><topic>Targeted therapy</topic><topic>Versatile cargoes</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Shim, Gayong</creatorcontrib><creatorcontrib>Park, Jinwon</creatorcontrib><creatorcontrib>Kim, Mi-Gyeong</creatorcontrib><creatorcontrib>Yang, Geon</creatorcontrib><creatorcontrib>Lee, Yeon</creatorcontrib><creatorcontrib>Oh, Yu-Kyoung</creatorcontrib><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Nanomedicine</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Shim, Gayong</au><au>Park, Jinwon</au><au>Kim, Mi-Gyeong</au><au>Yang, Geon</au><au>Lee, Yeon</au><au>Oh, Yu-Kyoung</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Noncovalent tethering of nucleic acid aptamer on DNA nanostructure for targeted photo/chemo/gene therapies</atitle><jtitle>Nanomedicine</jtitle><addtitle>Nanomedicine</addtitle><date>2020-02</date><risdate>2020</risdate><volume>24</volume><spage>102053</spage><epage>102053</epage><pages>102053-102053</pages><artnum>102053</artnum><issn>1549-9634</issn><eissn>1549-9642</eissn><abstract>Here, we report various therapeutic cargo-loadable DNA nanostructures that are shelled in polydopamine and noncovalently tethered with cancer cell-targeting DNA aptamers. Initial DNA nanostructure was formed by rolling-circle amplification and condensation with Mu peptides. This DNA nanostructure was loaded with an antisense oligonucleotide, a photosensitizer, or an anticancer chemotherapeutic drug. Each therapeutic agent-loaded DNA nanostructure was then shelled with polydopamine (PDA), and noncovalently decorated with a poly adenine-tailed nucleic acid aptamer (PA) specific for PTK7 receptor, resulting in PA-tethered and PDA-shelled DNA nanostructure (PA/PDN). PDA coating shell enabled photothermal therapy. In the cells overexpressing PTK7 receptor, photosensitizer-loaded PA/PDN showed greater photodynamic activity. Doxorubicin-loaded PA/PDN exerted higher anticancer activity than the other groups. Antisense oligonucleotide-loaded PA/PDN provided selective reduction of target proteins compared with other groups. Our results suggest that the PA-tethered and PDA-shelled DNA nanostructures could enable the specific receptor-targeted phototherapy, chemotherapy, and gene therapy against cancer cells.
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| subjects | DNA nanostructure Noncovalent DNA aptamer anchoring Polydopamine shell Targeted therapy Versatile cargoes |
| title | Noncovalent tethering of nucleic acid aptamer on DNA nanostructure for targeted photo/chemo/gene therapies |
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