Maternal HLA‐DR, HLA‐DQ, and HLA‐DP loci are linked with altered risk of recurrent pregnancy loss in Lebanese women: A case‐control study
Problem We investigated the association between idiopathic recurrent pregnancy loss (RPL) and HLA‐DPB1, HLA‐DQB1, and HLA‐DRB1 alleles and DPB1‐DQB1‐DRB1 haplotypes. Method of study Case‐control retrospective study involved 93 Lebanese women with unexplained RPL, and 113 multiparous Lebanese women w...
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| Veröffentlicht in: | American journal of reproductive immunology (1989) 2019-10, Vol.82 (4), p.e13173-n/a |
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| container_title | American journal of reproductive immunology (1989) |
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| creator | Aimagambetova, Gulzhanat Hajjej, Abdelhafidh Malalla, Zainab H. Finan, Ramzi R. Sarray, Sameh Almawi, Wassim Y. |
| description | Problem
We investigated the association between idiopathic recurrent pregnancy loss (RPL) and HLA‐DPB1, HLA‐DQB1, and HLA‐DRB1 alleles and DPB1‐DQB1‐DRB1 haplotypes.
Method of study
Case‐control retrospective study involved 93 Lebanese women with unexplained RPL, and 113 multiparous Lebanese women with two or more successful pregnancies, and no miscarriages who served as controls. DPB1, DQB1, and DRB1 genotyping was performed by PCR‐SSP.
Results
Expected and observed DRB1, DQB1, and DPB1 frequencies were comparable, and HLA genotype frequencies were in Hardy‐Weinberg equilibrium. Significantly higher frequencies of DRB1*04:01:01 and DRB1*08:01:01, and decreased DRB1*07:01:01 frequency were seen in RPL cases than in controls. On the other hand, the distribution of DQB1 alleles was comparable between cases and control groups. Significantly lower frequencies of DPB1*04:01:01 and DPB1*14:01:01 were seen in women with RPL than control subjects. While the frequency DPB1*02:01:01 was markedly higher in RPL cases than in controls, the difference was not significant. DPB1‐DQB1‐DRB1 haplotype analysis identified haplotype DPB1*04:01:01‐DQB1*03:02:01‐DRB1*04:01:01 to be positively associated, while haplotype DPB1*04:01:01‐DQB1*02:01:01‐DRB1*07:01:01 to be negatively associated with RPL. Of these two haplotypes, only DPB1*04:01:01‐DQB1*02:01:01‐DRB1*07:01:01 remained significant after correction for multiple tests (Pc = .0008).
Conclusion
Our results confirm an association of select DRB1 and DPB1 alleles with RPL in Lebanese women, and the first to identify DPB1‐DQB1‐DRB1 linked with altered RPL susceptibility, further highlighting the immunological/inflammatory nature of RPL.
Association of HLA class II alleles with altered risk of RPL among Lebanese women |
| doi_str_mv | 10.1111/aji.13173 |
| format | Article |
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We investigated the association between idiopathic recurrent pregnancy loss (RPL) and HLA‐DPB1, HLA‐DQB1, and HLA‐DRB1 alleles and DPB1‐DQB1‐DRB1 haplotypes.
Method of study
Case‐control retrospective study involved 93 Lebanese women with unexplained RPL, and 113 multiparous Lebanese women with two or more successful pregnancies, and no miscarriages who served as controls. DPB1, DQB1, and DRB1 genotyping was performed by PCR‐SSP.
Results
Expected and observed DRB1, DQB1, and DPB1 frequencies were comparable, and HLA genotype frequencies were in Hardy‐Weinberg equilibrium. Significantly higher frequencies of DRB1*04:01:01 and DRB1*08:01:01, and decreased DRB1*07:01:01 frequency were seen in RPL cases than in controls. On the other hand, the distribution of DQB1 alleles was comparable between cases and control groups. Significantly lower frequencies of DPB1*04:01:01 and DPB1*14:01:01 were seen in women with RPL than control subjects. While the frequency DPB1*02:01:01 was markedly higher in RPL cases than in controls, the difference was not significant. DPB1‐DQB1‐DRB1 haplotype analysis identified haplotype DPB1*04:01:01‐DQB1*03:02:01‐DRB1*04:01:01 to be positively associated, while haplotype DPB1*04:01:01‐DQB1*02:01:01‐DRB1*07:01:01 to be negatively associated with RPL. Of these two haplotypes, only DPB1*04:01:01‐DQB1*02:01:01‐DRB1*07:01:01 remained significant after correction for multiple tests (Pc = .0008).
Conclusion
Our results confirm an association of select DRB1 and DPB1 alleles with RPL in Lebanese women, and the first to identify DPB1‐DQB1‐DRB1 linked with altered RPL susceptibility, further highlighting the immunological/inflammatory nature of RPL.
Association of HLA class II alleles with altered risk of RPL among Lebanese women</description><identifier>ISSN: 1046-7408</identifier><identifier>EISSN: 1600-0897</identifier><identifier>DOI: 10.1111/aji.13173</identifier><identifier>PMID: 31339184</identifier><language>eng</language><publisher>Denmark: Wiley Subscription Services, Inc</publisher><subject>Alleles ; Drb1 protein ; Genotypes ; Genotyping ; Haplotypes ; Histocompatibility antigen HLA ; HLA class II ; Inflammation ; Miscarriage ; Pregnancy ; recurrent pregnancy loss</subject><ispartof>American journal of reproductive immunology (1989), 2019-10, Vol.82 (4), p.e13173-n/a</ispartof><rights>2019 John Wiley & Sons A/S. Published by John Wiley & Sons Ltd</rights><rights>2019 John Wiley & Sons A/S. Published by John Wiley & Sons Ltd.</rights><rights>Copyright © 2019 John Wiley & Sons A/S. Published by John Wiley & Sons Ltd</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c3533-ea810efe0afdb4e5f1d3db5ac2b161da30f0b3510c34656e32cf3d95f9eabbe13</citedby><cites>FETCH-LOGICAL-c3533-ea810efe0afdb4e5f1d3db5ac2b161da30f0b3510c34656e32cf3d95f9eabbe13</cites><orcidid>0000-0003-1633-9757</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1111%2Faji.13173$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1111%2Faji.13173$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>314,780,784,1417,27924,27925,45574,45575</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/31339184$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Aimagambetova, Gulzhanat</creatorcontrib><creatorcontrib>Hajjej, Abdelhafidh</creatorcontrib><creatorcontrib>Malalla, Zainab H.</creatorcontrib><creatorcontrib>Finan, Ramzi R.</creatorcontrib><creatorcontrib>Sarray, Sameh</creatorcontrib><creatorcontrib>Almawi, Wassim Y.</creatorcontrib><title>Maternal HLA‐DR, HLA‐DQ, and HLA‐DP loci are linked with altered risk of recurrent pregnancy loss in Lebanese women: A case‐control study</title><title>American journal of reproductive immunology (1989)</title><addtitle>Am J Reprod Immunol</addtitle><description>Problem
We investigated the association between idiopathic recurrent pregnancy loss (RPL) and HLA‐DPB1, HLA‐DQB1, and HLA‐DRB1 alleles and DPB1‐DQB1‐DRB1 haplotypes.
Method of study
Case‐control retrospective study involved 93 Lebanese women with unexplained RPL, and 113 multiparous Lebanese women with two or more successful pregnancies, and no miscarriages who served as controls. DPB1, DQB1, and DRB1 genotyping was performed by PCR‐SSP.
Results
Expected and observed DRB1, DQB1, and DPB1 frequencies were comparable, and HLA genotype frequencies were in Hardy‐Weinberg equilibrium. Significantly higher frequencies of DRB1*04:01:01 and DRB1*08:01:01, and decreased DRB1*07:01:01 frequency were seen in RPL cases than in controls. On the other hand, the distribution of DQB1 alleles was comparable between cases and control groups. Significantly lower frequencies of DPB1*04:01:01 and DPB1*14:01:01 were seen in women with RPL than control subjects. While the frequency DPB1*02:01:01 was markedly higher in RPL cases than in controls, the difference was not significant. DPB1‐DQB1‐DRB1 haplotype analysis identified haplotype DPB1*04:01:01‐DQB1*03:02:01‐DRB1*04:01:01 to be positively associated, while haplotype DPB1*04:01:01‐DQB1*02:01:01‐DRB1*07:01:01 to be negatively associated with RPL. Of these two haplotypes, only DPB1*04:01:01‐DQB1*02:01:01‐DRB1*07:01:01 remained significant after correction for multiple tests (Pc = .0008).
Conclusion
Our results confirm an association of select DRB1 and DPB1 alleles with RPL in Lebanese women, and the first to identify DPB1‐DQB1‐DRB1 linked with altered RPL susceptibility, further highlighting the immunological/inflammatory nature of RPL.
Association of HLA class II alleles with altered risk of RPL among Lebanese women</description><subject>Alleles</subject><subject>Drb1 protein</subject><subject>Genotypes</subject><subject>Genotyping</subject><subject>Haplotypes</subject><subject>Histocompatibility antigen HLA</subject><subject>HLA class II</subject><subject>Inflammation</subject><subject>Miscarriage</subject><subject>Pregnancy</subject><subject>recurrent pregnancy loss</subject><issn>1046-7408</issn><issn>1600-0897</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2019</creationdate><recordtype>article</recordtype><recordid>eNp1kctOGzEUhq0KVG5d8AKVJTatxIA9Z67sIgoFFMRFZT3y2Metg-NJ7RlF2fEI8Ip9EhxCuqhUb3yO9Z1Ptn9C9jk74nEdi4k54sBL-EC2ecFYwqq63Ig1y4qkzFi1RXZCmDAWz6H8SLaAA9S8yrbJy7Xo0Tth6cV49Ofp-dv94bq6O6TCqXV3S20nDRUeqTXuERWdm_4XFTaOx8ab8Eg7TT3KwXt0PZ15_OmEk4s4GAI1jo6xFQ4D0nk3RXdCR1SKgFEuO9f7ztLQD2qxRza1sAE_ve-75OH87MfpRTK--X55OhonEnKABEXFGWpkQqs2w1xzBarNhUxbXnAlgGnWQs6ZhKzIC4RUalB1rmsUbYscdsmXlXfmu98Dhr6ZmiDR2njHbghNmhYAKasZRPTgH3TSDctPW1JVWWd5xvNIfV1R0scHe9TNzJup8IuGs2aZUxNzat5yiuznd-PQTlH9JdfBROB4BcyNxcX_Tc3o6nKlfAXbzZ-B</recordid><startdate>201910</startdate><enddate>201910</enddate><creator>Aimagambetova, Gulzhanat</creator><creator>Hajjej, Abdelhafidh</creator><creator>Malalla, Zainab H.</creator><creator>Finan, Ramzi R.</creator><creator>Sarray, Sameh</creator><creator>Almawi, Wassim Y.</creator><general>Wiley Subscription Services, Inc</general><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7T5</scope><scope>7U9</scope><scope>H94</scope><scope>K9.</scope><scope>7X8</scope><orcidid>https://orcid.org/0000-0003-1633-9757</orcidid></search><sort><creationdate>201910</creationdate><title>Maternal HLA‐DR, HLA‐DQ, and HLA‐DP loci are linked with altered risk of recurrent pregnancy loss in Lebanese women: A case‐control study</title><author>Aimagambetova, Gulzhanat ; Hajjej, Abdelhafidh ; Malalla, Zainab H. ; Finan, Ramzi R. ; Sarray, Sameh ; Almawi, Wassim Y.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c3533-ea810efe0afdb4e5f1d3db5ac2b161da30f0b3510c34656e32cf3d95f9eabbe13</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2019</creationdate><topic>Alleles</topic><topic>Drb1 protein</topic><topic>Genotypes</topic><topic>Genotyping</topic><topic>Haplotypes</topic><topic>Histocompatibility antigen HLA</topic><topic>HLA class II</topic><topic>Inflammation</topic><topic>Miscarriage</topic><topic>Pregnancy</topic><topic>recurrent pregnancy loss</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Aimagambetova, Gulzhanat</creatorcontrib><creatorcontrib>Hajjej, Abdelhafidh</creatorcontrib><creatorcontrib>Malalla, Zainab H.</creatorcontrib><creatorcontrib>Finan, Ramzi R.</creatorcontrib><creatorcontrib>Sarray, Sameh</creatorcontrib><creatorcontrib>Almawi, Wassim Y.</creatorcontrib><collection>PubMed</collection><collection>CrossRef</collection><collection>Immunology Abstracts</collection><collection>Virology and AIDS Abstracts</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>MEDLINE - Academic</collection><jtitle>American journal of reproductive immunology (1989)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Aimagambetova, Gulzhanat</au><au>Hajjej, Abdelhafidh</au><au>Malalla, Zainab H.</au><au>Finan, Ramzi R.</au><au>Sarray, Sameh</au><au>Almawi, Wassim Y.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Maternal HLA‐DR, HLA‐DQ, and HLA‐DP loci are linked with altered risk of recurrent pregnancy loss in Lebanese women: A case‐control study</atitle><jtitle>American journal of reproductive immunology (1989)</jtitle><addtitle>Am J Reprod Immunol</addtitle><date>2019-10</date><risdate>2019</risdate><volume>82</volume><issue>4</issue><spage>e13173</spage><epage>n/a</epage><pages>e13173-n/a</pages><issn>1046-7408</issn><eissn>1600-0897</eissn><abstract>Problem
We investigated the association between idiopathic recurrent pregnancy loss (RPL) and HLA‐DPB1, HLA‐DQB1, and HLA‐DRB1 alleles and DPB1‐DQB1‐DRB1 haplotypes.
Method of study
Case‐control retrospective study involved 93 Lebanese women with unexplained RPL, and 113 multiparous Lebanese women with two or more successful pregnancies, and no miscarriages who served as controls. DPB1, DQB1, and DRB1 genotyping was performed by PCR‐SSP.
Results
Expected and observed DRB1, DQB1, and DPB1 frequencies were comparable, and HLA genotype frequencies were in Hardy‐Weinberg equilibrium. Significantly higher frequencies of DRB1*04:01:01 and DRB1*08:01:01, and decreased DRB1*07:01:01 frequency were seen in RPL cases than in controls. On the other hand, the distribution of DQB1 alleles was comparable between cases and control groups. Significantly lower frequencies of DPB1*04:01:01 and DPB1*14:01:01 were seen in women with RPL than control subjects. While the frequency DPB1*02:01:01 was markedly higher in RPL cases than in controls, the difference was not significant. DPB1‐DQB1‐DRB1 haplotype analysis identified haplotype DPB1*04:01:01‐DQB1*03:02:01‐DRB1*04:01:01 to be positively associated, while haplotype DPB1*04:01:01‐DQB1*02:01:01‐DRB1*07:01:01 to be negatively associated with RPL. Of these two haplotypes, only DPB1*04:01:01‐DQB1*02:01:01‐DRB1*07:01:01 remained significant after correction for multiple tests (Pc = .0008).
Conclusion
Our results confirm an association of select DRB1 and DPB1 alleles with RPL in Lebanese women, and the first to identify DPB1‐DQB1‐DRB1 linked with altered RPL susceptibility, further highlighting the immunological/inflammatory nature of RPL.
Association of HLA class II alleles with altered risk of RPL among Lebanese women</abstract><cop>Denmark</cop><pub>Wiley Subscription Services, Inc</pub><pmid>31339184</pmid><doi>10.1111/aji.13173</doi><tpages>7</tpages><orcidid>https://orcid.org/0000-0003-1633-9757</orcidid></addata></record> |
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| identifier | ISSN: 1046-7408 |
| ispartof | American journal of reproductive immunology (1989), 2019-10, Vol.82 (4), p.e13173-n/a |
| issn | 1046-7408 1600-0897 |
| language | eng |
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| source | Access via Wiley Online Library |
| subjects | Alleles Drb1 protein Genotypes Genotyping Haplotypes Histocompatibility antigen HLA HLA class II Inflammation Miscarriage Pregnancy recurrent pregnancy loss |
| title | Maternal HLA‐DR, HLA‐DQ, and HLA‐DP loci are linked with altered risk of recurrent pregnancy loss in Lebanese women: A case‐control study |
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