Biochemical characterization of a highly active ADP-dependent phosphofructokinase from Thermococcus kodakarensis

The genome sequence of Thermococcus kodakarensis contains an open reading frame, TK0376, annotated as ADP-dependent phosphofructokinase belonging to pfkC family. The encoding gene was expressed in Escherichia coli and the gene product was characterized. The recombinant protein was produced in solubl...

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Veröffentlicht in:	Journal of bioscience and bioengineering 2020-01, Vol.129 (1), p.6-15
Hauptverfasser:	Shakir, Nisar A., Bibi, Tahira, Aslam, Mehwish, Rashid, Naeem
Format:	Artikel
Sprache:	eng
Schlagworte:	Allosteric regulation Archaeal Proteins - chemistry Archaeal Proteins - genetics Archaeal Proteins - metabolism Bidirectional Bifunctional Enzyme Stability Euryarchaeota Fructosephosphates - metabolism Gluconeogenesis Glucose - metabolism Glycolysis Hydrogen-Ion Concentration Kinetics Phosphofructokinase Phosphorylation Phosphotransferases (Alcohol Group Acceptor) - chemistry Phosphotransferases (Alcohol Group Acceptor) - genetics Phosphotransferases (Alcohol Group Acceptor) - metabolism Recombinant Proteins - metabolism Thermococcus - chemistry Thermococcus - enzymology Thermococcus - genetics Thermococcus - metabolism
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