A mitochondrial delivery system using liposome-based nanocarriers that target myoblast cells
Mitochondrial function is reduced in skeletal muscles of many patients with systemic diseases and it is difficult to deliver medicinal substances to mitochondria in such tissue. In this study, we report on attempts to develop liposome-based carriers for mitochondrial delivery using mouse myoblasts (...
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| Veröffentlicht in: | Mitochondrion 2019-11, Vol.49, p.66-72 |
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| container_title | Mitochondrion |
| container_volume | 49 |
| creator | Katayama, Takashi Kinugawa, Shintaro Takada, Shingo Furihata, Takaaki Fukushima, Arata Yokota, Takashi Anzai, Toshihisa Hibino, Mitsue Harashima, Hideyoshi Yamada, Yuma |
| description | Mitochondrial function is reduced in skeletal muscles of many patients with systemic diseases and it is difficult to deliver medicinal substances to mitochondria in such tissue. In this study, we report on attempts to develop liposome-based carriers for mitochondrial delivery using mouse myoblasts (C2C12) by varying the lipid composition of the carriers. We found that a liposome that contains an optimal lipid modified with the KALA peptide (a cellular uptake and mitochondrial targeting device) was the most effective nanocarrier for achieving mitochondrial delivery in C2C12 cells. We also report on successful mitochondrial transgene expression using the carriers encapsulating a mitochondrial DNA vector as we previously reported.
•We succeeded in developing a nano carrier for mitochondrial delivery using myoblasts.•We also report on successful mitochondrial transgene expression using this carrier.•A KALA peptide modification to a liposome was effective for mitochondrial delivery. |
| doi_str_mv | 10.1016/j.mito.2019.07.005 |
| format | Article |
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•We succeeded in developing a nano carrier for mitochondrial delivery using myoblasts.•We also report on successful mitochondrial transgene expression using this carrier.•A KALA peptide modification to a liposome was effective for mitochondrial delivery.</description><identifier>ISSN: 1567-7249</identifier><identifier>EISSN: 1872-8278</identifier><identifier>DOI: 10.1016/j.mito.2019.07.005</identifier><identifier>PMID: 31326598</identifier><language>eng</language><publisher>Netherlands: Elsevier B.V</publisher><subject>Animals ; C2C12 myoblast cell line ; Cell Line ; Gene Transfer Techniques ; Liposome ; Liposomes ; Mice ; MITO-Porter ; Mitochondria ; Mitochondria, Muscle - genetics ; Mitochondria, Muscle - metabolism ; Mitochondrial gene delivery ; Mitochondrial transgene expression ; Myoblasts - cytology ; Myoblasts - metabolism ; Nanostructures - chemistry</subject><ispartof>Mitochondrion, 2019-11, Vol.49, p.66-72</ispartof><rights>2019 Elsevier B.V. and Mitochondria Research Society</rights><rights>Copyright © 2019 Elsevier B.V. and Mitochondria Research Society. All rights reserved.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c356t-ea8b3a03965b1312c547f46922c482225e033ab9cfc822718c1a641715554f4d3</citedby><cites>FETCH-LOGICAL-c356t-ea8b3a03965b1312c547f46922c482225e033ab9cfc822718c1a641715554f4d3</cites><orcidid>0000-0002-1568-9547 ; 0000-0001-5470-6648</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/j.mito.2019.07.005$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,776,780,3536,27903,27904,45974</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/31326598$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Katayama, Takashi</creatorcontrib><creatorcontrib>Kinugawa, Shintaro</creatorcontrib><creatorcontrib>Takada, Shingo</creatorcontrib><creatorcontrib>Furihata, Takaaki</creatorcontrib><creatorcontrib>Fukushima, Arata</creatorcontrib><creatorcontrib>Yokota, Takashi</creatorcontrib><creatorcontrib>Anzai, Toshihisa</creatorcontrib><creatorcontrib>Hibino, Mitsue</creatorcontrib><creatorcontrib>Harashima, Hideyoshi</creatorcontrib><creatorcontrib>Yamada, Yuma</creatorcontrib><title>A mitochondrial delivery system using liposome-based nanocarriers that target myoblast cells</title><title>Mitochondrion</title><addtitle>Mitochondrion</addtitle><description>Mitochondrial function is reduced in skeletal muscles of many patients with systemic diseases and it is difficult to deliver medicinal substances to mitochondria in such tissue. In this study, we report on attempts to develop liposome-based carriers for mitochondrial delivery using mouse myoblasts (C2C12) by varying the lipid composition of the carriers. We found that a liposome that contains an optimal lipid modified with the KALA peptide (a cellular uptake and mitochondrial targeting device) was the most effective nanocarrier for achieving mitochondrial delivery in C2C12 cells. We also report on successful mitochondrial transgene expression using the carriers encapsulating a mitochondrial DNA vector as we previously reported.
•We succeeded in developing a nano carrier for mitochondrial delivery using myoblasts.•We also report on successful mitochondrial transgene expression using this carrier.•A KALA peptide modification to a liposome was effective for mitochondrial delivery.</description><subject>Animals</subject><subject>C2C12 myoblast cell line</subject><subject>Cell Line</subject><subject>Gene Transfer Techniques</subject><subject>Liposome</subject><subject>Liposomes</subject><subject>Mice</subject><subject>MITO-Porter</subject><subject>Mitochondria</subject><subject>Mitochondria, Muscle - genetics</subject><subject>Mitochondria, Muscle - metabolism</subject><subject>Mitochondrial gene delivery</subject><subject>Mitochondrial transgene expression</subject><subject>Myoblasts - cytology</subject><subject>Myoblasts - metabolism</subject><subject>Nanostructures - chemistry</subject><issn>1567-7249</issn><issn>1872-8278</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2019</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9kEtr3TAQhUVpaB7tH-iiaJmNHT0syYZsQmibQCCbZBcQsjxOdLGtG41u4P77yNyky65mBs45zPkI-clZzRnXF5t6DjnWgvGuZqZmTH0hJ7w1omqFab-WXWlTGdF0x-QUccMYN1yIb-RYcim06toT8nRF1xD_EpchBTfRAabwBmlPcY8ZZrrDsDzTKWwjxhmq3iEMdHFL9C6lAAlpfnGZZpeeIdN5H_vJYaYepgm_k6PRTQg_PuYZefzz--H6prq7_3t7fXVXeal0rsC1vXRMdlr1XHLhVWPGRndC-KYVQihgUrq-86Mvp-Gt5043pYtSqhmbQZ6R80PuNsXXHWC2c8D1A7dA3KEVQvPO6LZTRSoOUp8iYoLRblOYXdpbzuxK1W7sCsSuVC0ztlAtpl8f-bt-huGf5RNjEVweBFBavhUqFn2AxcMQEvhshxj-l_8Ok9SJXQ</recordid><startdate>201911</startdate><enddate>201911</enddate><creator>Katayama, Takashi</creator><creator>Kinugawa, Shintaro</creator><creator>Takada, Shingo</creator><creator>Furihata, Takaaki</creator><creator>Fukushima, Arata</creator><creator>Yokota, Takashi</creator><creator>Anzai, Toshihisa</creator><creator>Hibino, Mitsue</creator><creator>Harashima, Hideyoshi</creator><creator>Yamada, Yuma</creator><general>Elsevier B.V</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><orcidid>https://orcid.org/0000-0002-1568-9547</orcidid><orcidid>https://orcid.org/0000-0001-5470-6648</orcidid></search><sort><creationdate>201911</creationdate><title>A mitochondrial delivery system using liposome-based nanocarriers that target myoblast cells</title><author>Katayama, Takashi ; Kinugawa, Shintaro ; Takada, Shingo ; Furihata, Takaaki ; Fukushima, Arata ; Yokota, Takashi ; Anzai, Toshihisa ; Hibino, Mitsue ; Harashima, Hideyoshi ; Yamada, Yuma</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c356t-ea8b3a03965b1312c547f46922c482225e033ab9cfc822718c1a641715554f4d3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2019</creationdate><topic>Animals</topic><topic>C2C12 myoblast cell line</topic><topic>Cell Line</topic><topic>Gene Transfer Techniques</topic><topic>Liposome</topic><topic>Liposomes</topic><topic>Mice</topic><topic>MITO-Porter</topic><topic>Mitochondria</topic><topic>Mitochondria, Muscle - genetics</topic><topic>Mitochondria, Muscle - metabolism</topic><topic>Mitochondrial gene delivery</topic><topic>Mitochondrial transgene expression</topic><topic>Myoblasts - cytology</topic><topic>Myoblasts - metabolism</topic><topic>Nanostructures - chemistry</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Katayama, Takashi</creatorcontrib><creatorcontrib>Kinugawa, Shintaro</creatorcontrib><creatorcontrib>Takada, Shingo</creatorcontrib><creatorcontrib>Furihata, Takaaki</creatorcontrib><creatorcontrib>Fukushima, Arata</creatorcontrib><creatorcontrib>Yokota, Takashi</creatorcontrib><creatorcontrib>Anzai, Toshihisa</creatorcontrib><creatorcontrib>Hibino, Mitsue</creatorcontrib><creatorcontrib>Harashima, Hideyoshi</creatorcontrib><creatorcontrib>Yamada, Yuma</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Mitochondrion</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Katayama, Takashi</au><au>Kinugawa, Shintaro</au><au>Takada, Shingo</au><au>Furihata, Takaaki</au><au>Fukushima, Arata</au><au>Yokota, Takashi</au><au>Anzai, Toshihisa</au><au>Hibino, Mitsue</au><au>Harashima, Hideyoshi</au><au>Yamada, Yuma</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>A mitochondrial delivery system using liposome-based nanocarriers that target myoblast cells</atitle><jtitle>Mitochondrion</jtitle><addtitle>Mitochondrion</addtitle><date>2019-11</date><risdate>2019</risdate><volume>49</volume><spage>66</spage><epage>72</epage><pages>66-72</pages><issn>1567-7249</issn><eissn>1872-8278</eissn><abstract>Mitochondrial function is reduced in skeletal muscles of many patients with systemic diseases and it is difficult to deliver medicinal substances to mitochondria in such tissue. In this study, we report on attempts to develop liposome-based carriers for mitochondrial delivery using mouse myoblasts (C2C12) by varying the lipid composition of the carriers. We found that a liposome that contains an optimal lipid modified with the KALA peptide (a cellular uptake and mitochondrial targeting device) was the most effective nanocarrier for achieving mitochondrial delivery in C2C12 cells. We also report on successful mitochondrial transgene expression using the carriers encapsulating a mitochondrial DNA vector as we previously reported.
•We succeeded in developing a nano carrier for mitochondrial delivery using myoblasts.•We also report on successful mitochondrial transgene expression using this carrier.•A KALA peptide modification to a liposome was effective for mitochondrial delivery.</abstract><cop>Netherlands</cop><pub>Elsevier B.V</pub><pmid>31326598</pmid><doi>10.1016/j.mito.2019.07.005</doi><tpages>7</tpages><orcidid>https://orcid.org/0000-0002-1568-9547</orcidid><orcidid>https://orcid.org/0000-0001-5470-6648</orcidid></addata></record> |
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| subjects | Animals C2C12 myoblast cell line Cell Line Gene Transfer Techniques Liposome Liposomes Mice MITO-Porter Mitochondria Mitochondria, Muscle - genetics Mitochondria, Muscle - metabolism Mitochondrial gene delivery Mitochondrial transgene expression Myoblasts - cytology Myoblasts - metabolism Nanostructures - chemistry |
| title | A mitochondrial delivery system using liposome-based nanocarriers that target myoblast cells |
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