Self-Assembly and Biogenesis of the Cellular Membrane are Dictated by Membrane Stretch and Composition
The cell plasma membrane is a highly dynamic organelle governing a wide range of cellular activities including ion transport, secretion, cell division, growth, and development. The fundamental process involved in the addition of new membranes to pre-existing plasma membranes, however, is unclear. He...
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| Veröffentlicht in: | The journal of physical chemistry. B 2019-08, Vol.123 (32), p.6997-7005 |
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| container_issue | 32 |
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| container_title | The journal of physical chemistry. B |
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| creator | Naik, Akshata R Kuhn, Eric R Lewis, Kenneth T Kokotovich, Keith M Maddipati, Krishna R Chen, Xuequn Hörber, J. H. K Taatjes, Douglas J Potoff, Jeffrey J Jena, Bhanu P |
| description | The cell plasma membrane is a highly dynamic organelle governing a wide range of cellular activities including ion transport, secretion, cell division, growth, and development. The fundamental process involved in the addition of new membranes to pre-existing plasma membranes, however, is unclear. Here, we report, using biophysical, morphological, biochemical, and molecular dynamic simulations, the selective incorporation of proteins and lipids from the cytosol into the cell plasma membrane dictated by membrane stretch and composition. Stretching of the cell membrane as a consequence of volume increase following incubation in a hypotonic solution and results in the incorporation of cytosolic proteins and lipids into the existing plasma membrane. Molecular dynamic simulations further confirm that increased membrane stretch results in the rapid insertion of lipids into the existing plasma membrane. Similarly, depletion of cholesterol from the cell plasma membrane selectively alters the incorporation of lipids into the membrane. |
| doi_str_mv | 10.1021/acs.jpcb.9b04769 |
| format | Article |
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Stretching of the cell membrane as a consequence of volume increase following incubation in a hypotonic solution and results in the incorporation of cytosolic proteins and lipids into the existing plasma membrane. Molecular dynamic simulations further confirm that increased membrane stretch results in the rapid insertion of lipids into the existing plasma membrane. 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H. K</creatorcontrib><creatorcontrib>Taatjes, Douglas J</creatorcontrib><creatorcontrib>Potoff, Jeffrey J</creatorcontrib><creatorcontrib>Jena, Bhanu P</creatorcontrib><title>Self-Assembly and Biogenesis of the Cellular Membrane are Dictated by Membrane Stretch and Composition</title><title>The journal of physical chemistry. B</title><addtitle>J. Phys. Chem. B</addtitle><description>The cell plasma membrane is a highly dynamic organelle governing a wide range of cellular activities including ion transport, secretion, cell division, growth, and development. The fundamental process involved in the addition of new membranes to pre-existing plasma membranes, however, is unclear. Here, we report, using biophysical, morphological, biochemical, and molecular dynamic simulations, the selective incorporation of proteins and lipids from the cytosol into the cell plasma membrane dictated by membrane stretch and composition. Stretching of the cell membrane as a consequence of volume increase following incubation in a hypotonic solution and results in the incorporation of cytosolic proteins and lipids into the existing plasma membrane. Molecular dynamic simulations further confirm that increased membrane stretch results in the rapid insertion of lipids into the existing plasma membrane. Similarly, depletion of cholesterol from the cell plasma membrane selectively alters the incorporation of lipids into the membrane.</description><subject>Animals</subject><subject>Blood Proteins - metabolism</subject><subject>Cell Membrane - chemistry</subject><subject>Cell Membrane - metabolism</subject><subject>Cytosol - metabolism</subject><subject>Erythrocytes - metabolism</subject><subject>Insulinoma - metabolism</subject><subject>Membrane Lipids - metabolism</subject><subject>Mice</subject><subject>Molecular Dynamics Simulation</subject><subject>Pancreatic Neoplasms - metabolism</subject><subject>Proteome - analysis</subject><subject>Proteome - metabolism</subject><subject>Rats</subject><subject>Rats, Sprague-Dawley</subject><subject>Tumor Cells, Cultured</subject><issn>1520-6106</issn><issn>1520-5207</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2019</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp1kLtPwzAQxi0EoqWwMyGPDKT4kTjNWMJTKmIozNHZvdBUSVzsZOh_j_sAJobTnXS_79PdR8glZ2POBL8F48ertdHjTLM4VdkRGfJEsChUenyYFWdqQM68XzEmEjFRp2QguRRikrEhKedYl9HUe2x0vaHQLuhdZT-xRV95akvaLZHmWNd9DY6-BspBixQc0vvKdNDhgurN32LeOezMcmeU22ZtfdVVtj0nJyXUHi8OfUQ-Hh_e8-do9vb0kk9nEchUdlEMykgdQ5aliY4TEAKRax6X8UJqITWEiTMNKSSCQ3h4kkAaS6EUGI1GyRG53vuunf3q0XdFU3kTzg-32d4XQiieKSUSFlC2R42z3jssi7WrGnCbgrNim24R0i226RaHdIPk6uDe6wYXv4KfOANwswd2Utu7Njz7v983suGGSg</recordid><startdate>20190815</startdate><enddate>20190815</enddate><creator>Naik, Akshata R</creator><creator>Kuhn, Eric R</creator><creator>Lewis, Kenneth T</creator><creator>Kokotovich, Keith M</creator><creator>Maddipati, Krishna R</creator><creator>Chen, Xuequn</creator><creator>Hörber, J. 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| source | ACS Publications; MEDLINE |
| subjects | Animals Blood Proteins - metabolism Cell Membrane - chemistry Cell Membrane - metabolism Cytosol - metabolism Erythrocytes - metabolism Insulinoma - metabolism Membrane Lipids - metabolism Mice Molecular Dynamics Simulation Pancreatic Neoplasms - metabolism Proteome - analysis Proteome - metabolism Rats Rats, Sprague-Dawley Tumor Cells, Cultured |
| title | Self-Assembly and Biogenesis of the Cellular Membrane are Dictated by Membrane Stretch and Composition |
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