Oral pyogenic granulomas show MAPK/ERK signaling pathway activation, which occurs independently of BRAF, KRAS, HRAS, NRAS, GNA11, and GNA14 mutations
Background Pyogenic granuloma (PG) is a benign nodular lesion with a prominent vascular component which may affect different sites. Recently, BRAF, KRAS, HRAS, NRAS, GNA11, and GNA14 mutations were reported on PGs of the skin. The present study assessed the role of the MAPK/ERK pathway in oral PG pa...
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| Veröffentlicht in: | Journal of oral pathology & medicine 2019-11, Vol.48 (10), p.906-910 |
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| container_title | Journal of oral pathology & medicine |
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| creator | Pereira, Thaís dos Santos Fontes Amorim, Larissa Stefhanne Damasceno Pereira, Núbia Braga Vitório, Jéssica Gardone Duarte‐Andrade, Filipe Fideles Guimarães, Letícia Martins Diniz, Marina Gonçalves Gomes, Carolina Cavaliéri Gomez, Ricardo Santiago |
| description | Background
Pyogenic granuloma (PG) is a benign nodular lesion with a prominent vascular component which may affect different sites. Recently, BRAF, KRAS, HRAS, NRAS, GNA11, and GNA14 mutations were reported on PGs of the skin. The present study assessed the role of the MAPK/ERK pathway in oral PG pathogenesis.
Methods
Mutations in hotspot regions of genes involved in the MAPK/ERK pathway activation were investigated by Sanger sequencing. The expression of phospho‐ERK1/2 was evaluated by immunohistochemistry.
Results
Oral PGs did not show mutations in the sequenced regions of the genes BRAF, KRAS, HRAS, NRAS, GNA11, or GNA14. Our results also showed activation of the MAPK/ERK pathway demonstrated by phospho‐ERK1/2 immunohistochemical positivity.
Conclusions
Although oral PG shows MAPK/ERK pathway activation, the driver molecular event remains to be elucidated. |
| doi_str_mv | 10.1111/jop.12922 |
| format | Article |
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Pyogenic granuloma (PG) is a benign nodular lesion with a prominent vascular component which may affect different sites. Recently, BRAF, KRAS, HRAS, NRAS, GNA11, and GNA14 mutations were reported on PGs of the skin. The present study assessed the role of the MAPK/ERK pathway in oral PG pathogenesis.
Methods
Mutations in hotspot regions of genes involved in the MAPK/ERK pathway activation were investigated by Sanger sequencing. The expression of phospho‐ERK1/2 was evaluated by immunohistochemistry.
Results
Oral PGs did not show mutations in the sequenced regions of the genes BRAF, KRAS, HRAS, NRAS, GNA11, or GNA14. Our results also showed activation of the MAPK/ERK pathway demonstrated by phospho‐ERK1/2 immunohistochemical positivity.
Conclusions
Although oral PG shows MAPK/ERK pathway activation, the driver molecular event remains to be elucidated.</description><identifier>ISSN: 0904-2512</identifier><identifier>EISSN: 1600-0714</identifier><identifier>DOI: 10.1111/jop.12922</identifier><identifier>PMID: 31310691</identifier><language>eng</language><publisher>Denmark: Wiley Subscription Services, Inc</publisher><subject>Adolescent ; Adult ; Aged ; capillary hemangioma ; Dentistry ; Extracellular signal-regulated kinase ; Female ; Granuloma ; Granuloma, Pyogenic - genetics ; Granuloma, Pyogenic - metabolism ; Granulomas ; GTP Phosphohydrolases - metabolism ; GTP-Binding Protein alpha Subunits - metabolism ; GTP-Binding Protein alpha Subunits, Gq-G11 - metabolism ; Humans ; Immunohistochemistry ; Kinases ; Male ; MAP kinase ; MAP Kinase Signaling System ; MAPK ; Membrane Proteins - metabolism ; Metabolic pathways ; Middle Aged ; Mutation ; oncogene ; Proto-Oncogene Proteins B-raf - metabolism ; Proto-Oncogene Proteins p21(ras) - metabolism ; pyogenic granuloma ; RAS ; Signal Transduction ; Young Adult</subject><ispartof>Journal of oral pathology & medicine, 2019-11, Vol.48 (10), p.906-910</ispartof><rights>2019 John Wiley & Sons A/S. Published by John Wiley & Sons Ltd</rights><rights>2019 John Wiley & Sons A/S. Published by John Wiley & Sons Ltd.</rights><rights>Copyright © 2019 John Wiley & Sons A/S. Published by John Wiley & Sons Ltd</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c3532-6639de93ff9882b9758f40634efd853f9015fc4d791fe2a5f9db0a34617eeb283</citedby><cites>FETCH-LOGICAL-c3532-6639de93ff9882b9758f40634efd853f9015fc4d791fe2a5f9db0a34617eeb283</cites><orcidid>0000-0002-1632-1533 ; 0000-0001-8770-8009 ; 0000-0002-6714-3124 ; 0000-0003-1580-4995</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1111%2Fjop.12922$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1111%2Fjop.12922$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>314,776,780,1411,27901,27902,45550,45551</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/31310691$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Pereira, Thaís dos Santos Fontes</creatorcontrib><creatorcontrib>Amorim, Larissa Stefhanne Damasceno</creatorcontrib><creatorcontrib>Pereira, Núbia Braga</creatorcontrib><creatorcontrib>Vitório, Jéssica Gardone</creatorcontrib><creatorcontrib>Duarte‐Andrade, Filipe Fideles</creatorcontrib><creatorcontrib>Guimarães, Letícia Martins</creatorcontrib><creatorcontrib>Diniz, Marina Gonçalves</creatorcontrib><creatorcontrib>Gomes, Carolina Cavaliéri</creatorcontrib><creatorcontrib>Gomez, Ricardo Santiago</creatorcontrib><title>Oral pyogenic granulomas show MAPK/ERK signaling pathway activation, which occurs independently of BRAF, KRAS, HRAS, NRAS, GNA11, and GNA14 mutations</title><title>Journal of oral pathology & medicine</title><addtitle>J Oral Pathol Med</addtitle><description>Background
Pyogenic granuloma (PG) is a benign nodular lesion with a prominent vascular component which may affect different sites. Recently, BRAF, KRAS, HRAS, NRAS, GNA11, and GNA14 mutations were reported on PGs of the skin. The present study assessed the role of the MAPK/ERK pathway in oral PG pathogenesis.
Methods
Mutations in hotspot regions of genes involved in the MAPK/ERK pathway activation were investigated by Sanger sequencing. The expression of phospho‐ERK1/2 was evaluated by immunohistochemistry.
Results
Oral PGs did not show mutations in the sequenced regions of the genes BRAF, KRAS, HRAS, NRAS, GNA11, or GNA14. Our results also showed activation of the MAPK/ERK pathway demonstrated by phospho‐ERK1/2 immunohistochemical positivity.
Conclusions
Although oral PG shows MAPK/ERK pathway activation, the driver molecular event remains to be elucidated.</description><subject>Adolescent</subject><subject>Adult</subject><subject>Aged</subject><subject>capillary hemangioma</subject><subject>Dentistry</subject><subject>Extracellular signal-regulated kinase</subject><subject>Female</subject><subject>Granuloma</subject><subject>Granuloma, Pyogenic - genetics</subject><subject>Granuloma, Pyogenic - metabolism</subject><subject>Granulomas</subject><subject>GTP Phosphohydrolases - metabolism</subject><subject>GTP-Binding Protein alpha Subunits - metabolism</subject><subject>GTP-Binding Protein alpha Subunits, Gq-G11 - metabolism</subject><subject>Humans</subject><subject>Immunohistochemistry</subject><subject>Kinases</subject><subject>Male</subject><subject>MAP kinase</subject><subject>MAP Kinase Signaling System</subject><subject>MAPK</subject><subject>Membrane Proteins - metabolism</subject><subject>Metabolic pathways</subject><subject>Middle Aged</subject><subject>Mutation</subject><subject>oncogene</subject><subject>Proto-Oncogene Proteins B-raf - metabolism</subject><subject>Proto-Oncogene Proteins p21(ras) - metabolism</subject><subject>pyogenic granuloma</subject><subject>RAS</subject><subject>Signal Transduction</subject><subject>Young Adult</subject><issn>0904-2512</issn><issn>1600-0714</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2019</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp1kctu1DAUhi0EokPbBS-ALLFppUnHx87Ny1D1AlM61QDryOPYMx4ldogTRnkQ3heTKSyQ8OL3WXznW5wfobdAriC8xd61V0A5pS_QDFJCIpJB_BLNCCdxRBOgJ-iN93tCIGMxvEYnDBiQlMMM_Vx1osbt6LbKGom3nbBD7Rrhsd-5A_5cPC0XN-sl9mZrRW3sFrei3x3EiIXszQ_RG2fn-LAzcoedlEPnsbGValUI29cjdhp_WBe3c7xcF1_m-H7KxynvHguAORa2msYYN0M_Cf0ZeqVF7dX583-Kvt3efL2-jx5Wdx-vi4dIsoTRKE0ZrxRnWvM8pxueJbmOScpipas8YZoTSLSMq4yDVlQkmlcbIlicQqbUhubsFF0cvW3nvg_K92VjvFR1Laxygy8pTThL8zyBgL7_B927oQs3CRQDCjwnWRqoyyMlO-d9p3TZdqYR3VgCKX93FbbacuoqsO-ejcOmUdVf8k85AVgcgYOp1fh_U_lp9XRU_gIM-ppg</recordid><startdate>201911</startdate><enddate>201911</enddate><creator>Pereira, Thaís dos Santos Fontes</creator><creator>Amorim, Larissa Stefhanne Damasceno</creator><creator>Pereira, Núbia Braga</creator><creator>Vitório, Jéssica Gardone</creator><creator>Duarte‐Andrade, Filipe Fideles</creator><creator>Guimarães, Letícia Martins</creator><creator>Diniz, Marina Gonçalves</creator><creator>Gomes, Carolina Cavaliéri</creator><creator>Gomez, Ricardo Santiago</creator><general>Wiley Subscription Services, Inc</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QP</scope><scope>7T5</scope><scope>H94</scope><scope>K9.</scope><scope>7X8</scope><orcidid>https://orcid.org/0000-0002-1632-1533</orcidid><orcidid>https://orcid.org/0000-0001-8770-8009</orcidid><orcidid>https://orcid.org/0000-0002-6714-3124</orcidid><orcidid>https://orcid.org/0000-0003-1580-4995</orcidid></search><sort><creationdate>201911</creationdate><title>Oral pyogenic granulomas show MAPK/ERK signaling pathway activation, which occurs independently of BRAF, KRAS, HRAS, NRAS, GNA11, and GNA14 mutations</title><author>Pereira, Thaís dos Santos Fontes ; Amorim, Larissa Stefhanne Damasceno ; Pereira, Núbia Braga ; Vitório, Jéssica Gardone ; Duarte‐Andrade, Filipe Fideles ; Guimarães, Letícia Martins ; Diniz, Marina Gonçalves ; Gomes, Carolina Cavaliéri ; Gomez, Ricardo Santiago</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c3532-6639de93ff9882b9758f40634efd853f9015fc4d791fe2a5f9db0a34617eeb283</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2019</creationdate><topic>Adolescent</topic><topic>Adult</topic><topic>Aged</topic><topic>capillary hemangioma</topic><topic>Dentistry</topic><topic>Extracellular signal-regulated kinase</topic><topic>Female</topic><topic>Granuloma</topic><topic>Granuloma, Pyogenic - genetics</topic><topic>Granuloma, Pyogenic - metabolism</topic><topic>Granulomas</topic><topic>GTP Phosphohydrolases - metabolism</topic><topic>GTP-Binding Protein alpha Subunits - metabolism</topic><topic>GTP-Binding Protein alpha Subunits, Gq-G11 - metabolism</topic><topic>Humans</topic><topic>Immunohistochemistry</topic><topic>Kinases</topic><topic>Male</topic><topic>MAP kinase</topic><topic>MAP Kinase Signaling System</topic><topic>MAPK</topic><topic>Membrane Proteins - metabolism</topic><topic>Metabolic pathways</topic><topic>Middle Aged</topic><topic>Mutation</topic><topic>oncogene</topic><topic>Proto-Oncogene Proteins B-raf - metabolism</topic><topic>Proto-Oncogene Proteins p21(ras) - metabolism</topic><topic>pyogenic granuloma</topic><topic>RAS</topic><topic>Signal Transduction</topic><topic>Young Adult</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Pereira, Thaís dos Santos Fontes</creatorcontrib><creatorcontrib>Amorim, Larissa Stefhanne Damasceno</creatorcontrib><creatorcontrib>Pereira, Núbia Braga</creatorcontrib><creatorcontrib>Vitório, Jéssica Gardone</creatorcontrib><creatorcontrib>Duarte‐Andrade, Filipe Fideles</creatorcontrib><creatorcontrib>Guimarães, Letícia Martins</creatorcontrib><creatorcontrib>Diniz, Marina Gonçalves</creatorcontrib><creatorcontrib>Gomes, Carolina Cavaliéri</creatorcontrib><creatorcontrib>Gomez, Ricardo Santiago</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Calcium & Calcified Tissue Abstracts</collection><collection>Immunology Abstracts</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>MEDLINE - Academic</collection><jtitle>Journal of oral pathology & medicine</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Pereira, Thaís dos Santos Fontes</au><au>Amorim, Larissa Stefhanne Damasceno</au><au>Pereira, Núbia Braga</au><au>Vitório, Jéssica Gardone</au><au>Duarte‐Andrade, Filipe Fideles</au><au>Guimarães, Letícia Martins</au><au>Diniz, Marina Gonçalves</au><au>Gomes, Carolina Cavaliéri</au><au>Gomez, Ricardo Santiago</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Oral pyogenic granulomas show MAPK/ERK signaling pathway activation, which occurs independently of BRAF, KRAS, HRAS, NRAS, GNA11, and GNA14 mutations</atitle><jtitle>Journal of oral pathology & medicine</jtitle><addtitle>J Oral Pathol Med</addtitle><date>2019-11</date><risdate>2019</risdate><volume>48</volume><issue>10</issue><spage>906</spage><epage>910</epage><pages>906-910</pages><issn>0904-2512</issn><eissn>1600-0714</eissn><abstract>Background
Pyogenic granuloma (PG) is a benign nodular lesion with a prominent vascular component which may affect different sites. Recently, BRAF, KRAS, HRAS, NRAS, GNA11, and GNA14 mutations were reported on PGs of the skin. The present study assessed the role of the MAPK/ERK pathway in oral PG pathogenesis.
Methods
Mutations in hotspot regions of genes involved in the MAPK/ERK pathway activation were investigated by Sanger sequencing. The expression of phospho‐ERK1/2 was evaluated by immunohistochemistry.
Results
Oral PGs did not show mutations in the sequenced regions of the genes BRAF, KRAS, HRAS, NRAS, GNA11, or GNA14. Our results also showed activation of the MAPK/ERK pathway demonstrated by phospho‐ERK1/2 immunohistochemical positivity.
Conclusions
Although oral PG shows MAPK/ERK pathway activation, the driver molecular event remains to be elucidated.</abstract><cop>Denmark</cop><pub>Wiley Subscription Services, Inc</pub><pmid>31310691</pmid><doi>10.1111/jop.12922</doi><tpages>5</tpages><orcidid>https://orcid.org/0000-0002-1632-1533</orcidid><orcidid>https://orcid.org/0000-0001-8770-8009</orcidid><orcidid>https://orcid.org/0000-0002-6714-3124</orcidid><orcidid>https://orcid.org/0000-0003-1580-4995</orcidid></addata></record> |
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| subjects | Adolescent Adult Aged capillary hemangioma Dentistry Extracellular signal-regulated kinase Female Granuloma Granuloma, Pyogenic - genetics Granuloma, Pyogenic - metabolism Granulomas GTP Phosphohydrolases - metabolism GTP-Binding Protein alpha Subunits - metabolism GTP-Binding Protein alpha Subunits, Gq-G11 - metabolism Humans Immunohistochemistry Kinases Male MAP kinase MAP Kinase Signaling System MAPK Membrane Proteins - metabolism Metabolic pathways Middle Aged Mutation oncogene Proto-Oncogene Proteins B-raf - metabolism Proto-Oncogene Proteins p21(ras) - metabolism pyogenic granuloma RAS Signal Transduction Young Adult |
| title | Oral pyogenic granulomas show MAPK/ERK signaling pathway activation, which occurs independently of BRAF, KRAS, HRAS, NRAS, GNA11, and GNA14 mutations |
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