Prevalence and genetic characterization of Giardia spp. and Cryptosporidium spp. in dogs in Iqaluit, Nunavut, Canada
There are few epidemiologic studies on the role of dogs in zoonotic parasitic transmission in the Circumpolar North. The objectives of this study were to: (a) estimate the faecal prevalence of Giardia spp. and Cryptosporidium spp. in dogs; (b) investigate potential associations between the type of d...
Gespeichert in:
| Veröffentlicht in: | Zoonoses and public health 2019-11, Vol.66 (7), p.813-825 |
|---|---|
| Hauptverfasser: | , , , , , , , , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 825 |
|---|---|
| container_issue | 7 |
| container_start_page | 813 |
| container_title | Zoonoses and public health |
| container_volume | 66 |
| creator | Julien, Danielle A. Sargeant, Jan M. Guy, Rebecca A. Shapiro, Karen Imai, Rachel K. Bunce, Anna Sudlovenick, Enooyaq Chen, Shu Li, Jiping Harper, Sherilee L. |
| description | There are few epidemiologic studies on the role of dogs in zoonotic parasitic transmission in the Circumpolar North. The objectives of this study were to: (a) estimate the faecal prevalence of Giardia spp. and Cryptosporidium spp. in dogs; (b) investigate potential associations between the type of dog population and the faecal presence of Giardia spp. and Cryptosporidium spp.; and (c) describe the molecular characteristics of Giardia spp. and Cryptosporidium spp. in dogs in Iqaluit, Nunavut. We conducted two cross‐sectional studies in July and September 2016. In July, the team collected daily faecal samples for 3 days from each of 20 sled dogs. In September, the team collected three faecal samples from each of 59 sled dogs, 111 samples from shelter dogs and 104 from community dogs. We analysed faecal samples for the presence of Giardia spp. and Cryptosporidium spp. using rapid immunoassay and flotation techniques. Polymerase chain reaction (PCR) and sequencing of target genes were performed on positive faecal samples. Overall, the faecal prevalence of at least one of the target parasites, when one faecal sample was chosen at random for all dogs, was 8.16% (CI: 5.52–11.92), and for Giardia spp. and Cryptosporidium spp., prevalence was 4.42% (CI: 2.58–7.49) and 6.12% (CI: 3.88–9.53), respectively. The odds of faecal Giardia spp. in sled dogs were significantly higher than those in shelter and community dogs (OR 10.19 [CI: 1.16–89.35]). Sequence analysis revealed that 6 faecal samples were Giardia intestinalis, zoonotic assemblage B (n = 2) and species‐specific assemblages D (n = 3) and E (n = 1), and five faecal samples were Cryptosporidium canis. Giardia intestinalis is zoonotic; however, Cryptosporidium canis is rare in humans and, when present, usually occurs in immunosuppressed individuals. Dogs may be a potential source of zoonotic Giardia intestinalis assemblage B infections in residents in Iqaluit, Nunavut, Canada; however, the direction of transmission is unclear. |
| doi_str_mv | 10.1111/zph.12628 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_2258163982</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>2258163982</sourcerecordid><originalsourceid>FETCH-LOGICAL-c3538-c191f625d82d3a2ac28b86feda357a0dd5fc895f6fdc0df116e29dbf3c5f12c53</originalsourceid><addsrcrecordid>eNp10ctKAzEUBuAgiveFLyADbhRszYVMM0sp2hZEXejGTTjNpY1MkzGZqbRP79SpLgSzOQfy8RPyI3RGcJ-052ZdzfuE5lTsoEMictajbCB2tzspeHGAjlJ6x5jzAg_20QEjDHNMi0NUP0ezhNJ4ZTLwOpsZb2qnMjWHCKo20a2hdsFnwWYjB1E7yFJV9b_xMK6qOqQqRKdds-gunM90mKXNnHxA2bj6OntsPCybdhmCBw0naM9Cmczpdh6j1_u7l-G49_A0mgxvH3qKcSZ6ihTE5pRrQTUDCoqKqcit0cD4ALDW3CpRcJtbrbC2hOSGFnpqmeKWUMXZMbrscqsYPhqTarlwSZmyBG9CkySlXJCcFYK29OIPfQ9N9O3rJGU4H1BO6EZddUrFkFI0VlbRLSCuJMFyU4Vsq5DfVbT2fJvYTBdG_8qfv2_BTQc-XWlW_yfJt-dxF_kFEwyTkg</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>2306725122</pqid></control><display><type>article</type><title>Prevalence and genetic characterization of Giardia spp. and Cryptosporidium spp. in dogs in Iqaluit, Nunavut, Canada</title><source>MEDLINE</source><source>Wiley Online Library Journals Frontfile Complete</source><creator>Julien, Danielle A. ; Sargeant, Jan M. ; Guy, Rebecca A. ; Shapiro, Karen ; Imai, Rachel K. ; Bunce, Anna ; Sudlovenick, Enooyaq ; Chen, Shu ; Li, Jiping ; Harper, Sherilee L.</creator><creatorcontrib>Julien, Danielle A. ; Sargeant, Jan M. ; Guy, Rebecca A. ; Shapiro, Karen ; Imai, Rachel K. ; Bunce, Anna ; Sudlovenick, Enooyaq ; Chen, Shu ; Li, Jiping ; Harper, Sherilee L.</creatorcontrib><description>There are few epidemiologic studies on the role of dogs in zoonotic parasitic transmission in the Circumpolar North. The objectives of this study were to: (a) estimate the faecal prevalence of Giardia spp. and Cryptosporidium spp. in dogs; (b) investigate potential associations between the type of dog population and the faecal presence of Giardia spp. and Cryptosporidium spp.; and (c) describe the molecular characteristics of Giardia spp. and Cryptosporidium spp. in dogs in Iqaluit, Nunavut. We conducted two cross‐sectional studies in July and September 2016. In July, the team collected daily faecal samples for 3 days from each of 20 sled dogs. In September, the team collected three faecal samples from each of 59 sled dogs, 111 samples from shelter dogs and 104 from community dogs. We analysed faecal samples for the presence of Giardia spp. and Cryptosporidium spp. using rapid immunoassay and flotation techniques. Polymerase chain reaction (PCR) and sequencing of target genes were performed on positive faecal samples. Overall, the faecal prevalence of at least one of the target parasites, when one faecal sample was chosen at random for all dogs, was 8.16% (CI: 5.52–11.92), and for Giardia spp. and Cryptosporidium spp., prevalence was 4.42% (CI: 2.58–7.49) and 6.12% (CI: 3.88–9.53), respectively. The odds of faecal Giardia spp. in sled dogs were significantly higher than those in shelter and community dogs (OR 10.19 [CI: 1.16–89.35]). Sequence analysis revealed that 6 faecal samples were Giardia intestinalis, zoonotic assemblage B (n = 2) and species‐specific assemblages D (n = 3) and E (n = 1), and five faecal samples were Cryptosporidium canis. Giardia intestinalis is zoonotic; however, Cryptosporidium canis is rare in humans and, when present, usually occurs in immunosuppressed individuals. Dogs may be a potential source of zoonotic Giardia intestinalis assemblage B infections in residents in Iqaluit, Nunavut, Canada; however, the direction of transmission is unclear.</description><identifier>ISSN: 1863-1959</identifier><identifier>EISSN: 1863-2378</identifier><identifier>DOI: 10.1111/zph.12628</identifier><identifier>PMID: 31305029</identifier><language>eng</language><publisher>Germany: Blackwell Publishing Ltd</publisher><subject>Animal behavior ; Animals ; Circumpolar North ; Communities ; Cryptosporidiosis - epidemiology ; Cryptosporidiosis - parasitology ; Cryptosporidium ; Cryptosporidium - classification ; Cryptosporidium - isolation & purification ; Cryptosporidium canis ; DNA ; dog ; Dog Diseases - epidemiology ; Dog Diseases - parasitology ; Dogs ; Epidemiology ; Feces - parasitology ; Flotation ; Gene sequencing ; Giardia ; Giardia - classification ; Giardia - isolation & purification ; Giardia intestinalis ; Giardiasis - epidemiology ; Giardiasis - parasitology ; Giardiasis - veterinary ; Immunoassay ; Immunoassays ; Nucleotide sequence ; Nunavut ; One Health ; Parasites ; PCR ; Polymerase chain reaction ; Protozoa ; Shelters ; Sleds ; Time Factors ; Zoonoses</subject><ispartof>Zoonoses and public health, 2019-11, Vol.66 (7), p.813-825</ispartof><rights>2019 Blackwell Verlag GmbH</rights><rights>2019 Blackwell Verlag GmbH.</rights><rights>Copyright © 2019 Blackwell Verlag GmbH</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c3538-c191f625d82d3a2ac28b86feda357a0dd5fc895f6fdc0df116e29dbf3c5f12c53</citedby><cites>FETCH-LOGICAL-c3538-c191f625d82d3a2ac28b86feda357a0dd5fc895f6fdc0df116e29dbf3c5f12c53</cites><orcidid>0000-0002-3369-2088 ; 0000-0001-7298-8765</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1111%2Fzph.12628$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1111%2Fzph.12628$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>314,778,782,1414,27907,27908,45557,45558</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/31305029$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Julien, Danielle A.</creatorcontrib><creatorcontrib>Sargeant, Jan M.</creatorcontrib><creatorcontrib>Guy, Rebecca A.</creatorcontrib><creatorcontrib>Shapiro, Karen</creatorcontrib><creatorcontrib>Imai, Rachel K.</creatorcontrib><creatorcontrib>Bunce, Anna</creatorcontrib><creatorcontrib>Sudlovenick, Enooyaq</creatorcontrib><creatorcontrib>Chen, Shu</creatorcontrib><creatorcontrib>Li, Jiping</creatorcontrib><creatorcontrib>Harper, Sherilee L.</creatorcontrib><title>Prevalence and genetic characterization of Giardia spp. and Cryptosporidium spp. in dogs in Iqaluit, Nunavut, Canada</title><title>Zoonoses and public health</title><addtitle>Zoonoses Public Health</addtitle><description>There are few epidemiologic studies on the role of dogs in zoonotic parasitic transmission in the Circumpolar North. The objectives of this study were to: (a) estimate the faecal prevalence of Giardia spp. and Cryptosporidium spp. in dogs; (b) investigate potential associations between the type of dog population and the faecal presence of Giardia spp. and Cryptosporidium spp.; and (c) describe the molecular characteristics of Giardia spp. and Cryptosporidium spp. in dogs in Iqaluit, Nunavut. We conducted two cross‐sectional studies in July and September 2016. In July, the team collected daily faecal samples for 3 days from each of 20 sled dogs. In September, the team collected three faecal samples from each of 59 sled dogs, 111 samples from shelter dogs and 104 from community dogs. We analysed faecal samples for the presence of Giardia spp. and Cryptosporidium spp. using rapid immunoassay and flotation techniques. Polymerase chain reaction (PCR) and sequencing of target genes were performed on positive faecal samples. Overall, the faecal prevalence of at least one of the target parasites, when one faecal sample was chosen at random for all dogs, was 8.16% (CI: 5.52–11.92), and for Giardia spp. and Cryptosporidium spp., prevalence was 4.42% (CI: 2.58–7.49) and 6.12% (CI: 3.88–9.53), respectively. The odds of faecal Giardia spp. in sled dogs were significantly higher than those in shelter and community dogs (OR 10.19 [CI: 1.16–89.35]). Sequence analysis revealed that 6 faecal samples were Giardia intestinalis, zoonotic assemblage B (n = 2) and species‐specific assemblages D (n = 3) and E (n = 1), and five faecal samples were Cryptosporidium canis. Giardia intestinalis is zoonotic; however, Cryptosporidium canis is rare in humans and, when present, usually occurs in immunosuppressed individuals. Dogs may be a potential source of zoonotic Giardia intestinalis assemblage B infections in residents in Iqaluit, Nunavut, Canada; however, the direction of transmission is unclear.</description><subject>Animal behavior</subject><subject>Animals</subject><subject>Circumpolar North</subject><subject>Communities</subject><subject>Cryptosporidiosis - epidemiology</subject><subject>Cryptosporidiosis - parasitology</subject><subject>Cryptosporidium</subject><subject>Cryptosporidium - classification</subject><subject>Cryptosporidium - isolation & purification</subject><subject>Cryptosporidium canis</subject><subject>DNA</subject><subject>dog</subject><subject>Dog Diseases - epidemiology</subject><subject>Dog Diseases - parasitology</subject><subject>Dogs</subject><subject>Epidemiology</subject><subject>Feces - parasitology</subject><subject>Flotation</subject><subject>Gene sequencing</subject><subject>Giardia</subject><subject>Giardia - classification</subject><subject>Giardia - isolation & purification</subject><subject>Giardia intestinalis</subject><subject>Giardiasis - epidemiology</subject><subject>Giardiasis - parasitology</subject><subject>Giardiasis - veterinary</subject><subject>Immunoassay</subject><subject>Immunoassays</subject><subject>Nucleotide sequence</subject><subject>Nunavut</subject><subject>One Health</subject><subject>Parasites</subject><subject>PCR</subject><subject>Polymerase chain reaction</subject><subject>Protozoa</subject><subject>Shelters</subject><subject>Sleds</subject><subject>Time Factors</subject><subject>Zoonoses</subject><issn>1863-1959</issn><issn>1863-2378</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2019</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp10ctKAzEUBuAgiveFLyADbhRszYVMM0sp2hZEXejGTTjNpY1MkzGZqbRP79SpLgSzOQfy8RPyI3RGcJ-052ZdzfuE5lTsoEMictajbCB2tzspeHGAjlJ6x5jzAg_20QEjDHNMi0NUP0ezhNJ4ZTLwOpsZb2qnMjWHCKo20a2hdsFnwWYjB1E7yFJV9b_xMK6qOqQqRKdds-gunM90mKXNnHxA2bj6OntsPCybdhmCBw0naM9Cmczpdh6j1_u7l-G49_A0mgxvH3qKcSZ6ihTE5pRrQTUDCoqKqcit0cD4ALDW3CpRcJtbrbC2hOSGFnpqmeKWUMXZMbrscqsYPhqTarlwSZmyBG9CkySlXJCcFYK29OIPfQ9N9O3rJGU4H1BO6EZddUrFkFI0VlbRLSCuJMFyU4Vsq5DfVbT2fJvYTBdG_8qfv2_BTQc-XWlW_yfJt-dxF_kFEwyTkg</recordid><startdate>201911</startdate><enddate>201911</enddate><creator>Julien, Danielle A.</creator><creator>Sargeant, Jan M.</creator><creator>Guy, Rebecca A.</creator><creator>Shapiro, Karen</creator><creator>Imai, Rachel K.</creator><creator>Bunce, Anna</creator><creator>Sudlovenick, Enooyaq</creator><creator>Chen, Shu</creator><creator>Li, Jiping</creator><creator>Harper, Sherilee L.</creator><general>Blackwell Publishing Ltd</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QL</scope><scope>7T2</scope><scope>7U7</scope><scope>7U9</scope><scope>C1K</scope><scope>F1W</scope><scope>H94</scope><scope>H95</scope><scope>K9.</scope><scope>L.G</scope><scope>M7N</scope><scope>7X8</scope><orcidid>https://orcid.org/0000-0002-3369-2088</orcidid><orcidid>https://orcid.org/0000-0001-7298-8765</orcidid></search><sort><creationdate>201911</creationdate><title>Prevalence and genetic characterization of Giardia spp. and Cryptosporidium spp. in dogs in Iqaluit, Nunavut, Canada</title><author>Julien, Danielle A. ; Sargeant, Jan M. ; Guy, Rebecca A. ; Shapiro, Karen ; Imai, Rachel K. ; Bunce, Anna ; Sudlovenick, Enooyaq ; Chen, Shu ; Li, Jiping ; Harper, Sherilee L.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c3538-c191f625d82d3a2ac28b86feda357a0dd5fc895f6fdc0df116e29dbf3c5f12c53</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2019</creationdate><topic>Animal behavior</topic><topic>Animals</topic><topic>Circumpolar North</topic><topic>Communities</topic><topic>Cryptosporidiosis - epidemiology</topic><topic>Cryptosporidiosis - parasitology</topic><topic>Cryptosporidium</topic><topic>Cryptosporidium - classification</topic><topic>Cryptosporidium - isolation & purification</topic><topic>Cryptosporidium canis</topic><topic>DNA</topic><topic>dog</topic><topic>Dog Diseases - epidemiology</topic><topic>Dog Diseases - parasitology</topic><topic>Dogs</topic><topic>Epidemiology</topic><topic>Feces - parasitology</topic><topic>Flotation</topic><topic>Gene sequencing</topic><topic>Giardia</topic><topic>Giardia - classification</topic><topic>Giardia - isolation & purification</topic><topic>Giardia intestinalis</topic><topic>Giardiasis - epidemiology</topic><topic>Giardiasis - parasitology</topic><topic>Giardiasis - veterinary</topic><topic>Immunoassay</topic><topic>Immunoassays</topic><topic>Nucleotide sequence</topic><topic>Nunavut</topic><topic>One Health</topic><topic>Parasites</topic><topic>PCR</topic><topic>Polymerase chain reaction</topic><topic>Protozoa</topic><topic>Shelters</topic><topic>Sleds</topic><topic>Time Factors</topic><topic>Zoonoses</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Julien, Danielle A.</creatorcontrib><creatorcontrib>Sargeant, Jan M.</creatorcontrib><creatorcontrib>Guy, Rebecca A.</creatorcontrib><creatorcontrib>Shapiro, Karen</creatorcontrib><creatorcontrib>Imai, Rachel K.</creatorcontrib><creatorcontrib>Bunce, Anna</creatorcontrib><creatorcontrib>Sudlovenick, Enooyaq</creatorcontrib><creatorcontrib>Chen, Shu</creatorcontrib><creatorcontrib>Li, Jiping</creatorcontrib><creatorcontrib>Harper, Sherilee L.</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Health and Safety Science Abstracts (Full archive)</collection><collection>Toxicology Abstracts</collection><collection>Virology and AIDS Abstracts</collection><collection>Environmental Sciences and Pollution Management</collection><collection>ASFA: Aquatic Sciences and Fisheries Abstracts</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>Aquatic Science & Fisheries Abstracts (ASFA) 1: Biological Sciences & Living Resources</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Aquatic Science & Fisheries Abstracts (ASFA) Professional</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>MEDLINE - Academic</collection><jtitle>Zoonoses and public health</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Julien, Danielle A.</au><au>Sargeant, Jan M.</au><au>Guy, Rebecca A.</au><au>Shapiro, Karen</au><au>Imai, Rachel K.</au><au>Bunce, Anna</au><au>Sudlovenick, Enooyaq</au><au>Chen, Shu</au><au>Li, Jiping</au><au>Harper, Sherilee L.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Prevalence and genetic characterization of Giardia spp. and Cryptosporidium spp. in dogs in Iqaluit, Nunavut, Canada</atitle><jtitle>Zoonoses and public health</jtitle><addtitle>Zoonoses Public Health</addtitle><date>2019-11</date><risdate>2019</risdate><volume>66</volume><issue>7</issue><spage>813</spage><epage>825</epage><pages>813-825</pages><issn>1863-1959</issn><eissn>1863-2378</eissn><abstract>There are few epidemiologic studies on the role of dogs in zoonotic parasitic transmission in the Circumpolar North. The objectives of this study were to: (a) estimate the faecal prevalence of Giardia spp. and Cryptosporidium spp. in dogs; (b) investigate potential associations between the type of dog population and the faecal presence of Giardia spp. and Cryptosporidium spp.; and (c) describe the molecular characteristics of Giardia spp. and Cryptosporidium spp. in dogs in Iqaluit, Nunavut. We conducted two cross‐sectional studies in July and September 2016. In July, the team collected daily faecal samples for 3 days from each of 20 sled dogs. In September, the team collected three faecal samples from each of 59 sled dogs, 111 samples from shelter dogs and 104 from community dogs. We analysed faecal samples for the presence of Giardia spp. and Cryptosporidium spp. using rapid immunoassay and flotation techniques. Polymerase chain reaction (PCR) and sequencing of target genes were performed on positive faecal samples. Overall, the faecal prevalence of at least one of the target parasites, when one faecal sample was chosen at random for all dogs, was 8.16% (CI: 5.52–11.92), and for Giardia spp. and Cryptosporidium spp., prevalence was 4.42% (CI: 2.58–7.49) and 6.12% (CI: 3.88–9.53), respectively. The odds of faecal Giardia spp. in sled dogs were significantly higher than those in shelter and community dogs (OR 10.19 [CI: 1.16–89.35]). Sequence analysis revealed that 6 faecal samples were Giardia intestinalis, zoonotic assemblage B (n = 2) and species‐specific assemblages D (n = 3) and E (n = 1), and five faecal samples were Cryptosporidium canis. Giardia intestinalis is zoonotic; however, Cryptosporidium canis is rare in humans and, when present, usually occurs in immunosuppressed individuals. Dogs may be a potential source of zoonotic Giardia intestinalis assemblage B infections in residents in Iqaluit, Nunavut, Canada; however, the direction of transmission is unclear.</abstract><cop>Germany</cop><pub>Blackwell Publishing Ltd</pub><pmid>31305029</pmid><doi>10.1111/zph.12628</doi><tpages>13</tpages><orcidid>https://orcid.org/0000-0002-3369-2088</orcidid><orcidid>https://orcid.org/0000-0001-7298-8765</orcidid></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 1863-1959 |
| ispartof | Zoonoses and public health, 2019-11, Vol.66 (7), p.813-825 |
| issn | 1863-1959 1863-2378 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_2258163982 |
| source | MEDLINE; Wiley Online Library Journals Frontfile Complete |
| subjects | Animal behavior Animals Circumpolar North Communities Cryptosporidiosis - epidemiology Cryptosporidiosis - parasitology Cryptosporidium Cryptosporidium - classification Cryptosporidium - isolation & purification Cryptosporidium canis DNA dog Dog Diseases - epidemiology Dog Diseases - parasitology Dogs Epidemiology Feces - parasitology Flotation Gene sequencing Giardia Giardia - classification Giardia - isolation & purification Giardia intestinalis Giardiasis - epidemiology Giardiasis - parasitology Giardiasis - veterinary Immunoassay Immunoassays Nucleotide sequence Nunavut One Health Parasites PCR Polymerase chain reaction Protozoa Shelters Sleds Time Factors Zoonoses |
| title | Prevalence and genetic characterization of Giardia spp. and Cryptosporidium spp. in dogs in Iqaluit, Nunavut, Canada |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-16T06%3A52%3A18IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Prevalence%20and%20genetic%20characterization%20of%20Giardia%20spp.%20and%20Cryptosporidium%20spp.%20in%20dogs%20in%20Iqaluit,%20Nunavut,%20Canada&rft.jtitle=Zoonoses%20and%20public%20health&rft.au=Julien,%20Danielle%20A.&rft.date=2019-11&rft.volume=66&rft.issue=7&rft.spage=813&rft.epage=825&rft.pages=813-825&rft.issn=1863-1959&rft.eissn=1863-2378&rft_id=info:doi/10.1111/zph.12628&rft_dat=%3Cproquest_cross%3E2258163982%3C/proquest_cross%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=2306725122&rft_id=info:pmid/31305029&rfr_iscdi=true |