Cationic penetrating antioxidants switch off Mn cluster of photosystem II in situ
Mitochondria-targeted antioxidants (also known as ‘Skulachev Ions’ electrophoretically accumulated by mitochondria) exert anti-ageing and ROS-protecting effects well documented in animal and human cells. However, their effects on chloroplast in photosynthetic cells and corresponding mechanisms are s...
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| Veröffentlicht in: | Photosynthesis research 2019-11, Vol.142 (2), p.229-240 |
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| creator | Ptushenko, Vasily V. Solovchenko, Alexei E. Bychkov, Andrew Y. Chivkunova, Olga B. Golovin, Andrey V. Gorelova, Olga A. Ismagulova, Tatiana T. Kulik, Leonid V. Lobakova, Elena S. Lukyanov, Alexandr A. Samoilova, Rima I. Scherbakov, Pavel N. Selyakh, Irina O. Semenova, Larisa R. Vasilieva, Svetlana G. Baulina, Olga I. Skulachev, Maxim V. Kirpichnikov, Mikhail P. |
| description | Mitochondria-targeted antioxidants (also known as ‘Skulachev Ions’ electrophoretically accumulated by mitochondria) exert anti-ageing and ROS-protecting effects well documented in animal and human cells. However, their effects on chloroplast in photosynthetic cells and corresponding mechanisms are scarcely known. For the first time, we describe a dramatic quenching effect of (10-(6-plastoquinonyl)decyl triphenylphosphonium (SkQ1) on chlorophyll fluorescence, apparently mediated by redox interaction of SkQ1 with Mn cluster in Photosystem II (PSII) of chlorophyte microalga
Chlorella vulgaris
and disabling the oxygen-evolving complex (OEC). Microalgal cells displayed a vigorous uptake of SkQ1 which internal concentration built up to a very high level. Using optical and EPR spectroscopy, as well as electron donors and in silico molecular simulation techniques, we found that SkQ1 molecule can interact with Mn atoms of the OEC in PSII. This stops water splitting giving rise to potent quencher(s), e.g. oxidized reaction centre of PSII. Other components of the photosynthetic apparatus proved to be mostly intact. This effect of the Skulachev ions might help to develop in vivo models of photosynthetic cells with impaired OEC function but essentially intact otherwise. The observed phenomenon suggests that SkQ1 can be applied to study stress-induced damages to OEC in photosynthetic organisms. |
| doi_str_mv | 10.1007/s11120-019-00657-2 |
| format | Article |
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Chlorella vulgaris
and disabling the oxygen-evolving complex (OEC). Microalgal cells displayed a vigorous uptake of SkQ1 which internal concentration built up to a very high level. Using optical and EPR spectroscopy, as well as electron donors and in silico molecular simulation techniques, we found that SkQ1 molecule can interact with Mn atoms of the OEC in PSII. This stops water splitting giving rise to potent quencher(s), e.g. oxidized reaction centre of PSII. Other components of the photosynthetic apparatus proved to be mostly intact. This effect of the Skulachev ions might help to develop in vivo models of photosynthetic cells with impaired OEC function but essentially intact otherwise. The observed phenomenon suggests that SkQ1 can be applied to study stress-induced damages to OEC in photosynthetic organisms.</description><identifier>ISSN: 0166-8595</identifier><identifier>EISSN: 1573-5079</identifier><identifier>DOI: 10.1007/s11120-019-00657-2</identifier><identifier>PMID: 31302832</identifier><language>eng</language><publisher>Dordrecht: Springer Netherlands</publisher><subject>Aging ; Antioxidants ; Antioxidants - metabolism ; Biochemistry ; Biomedical and Life Sciences ; Cations ; Cells ; Chlorella vulgaris - drug effects ; Chlorella vulgaris - metabolism ; Chlorophyll ; Chlorophyll - metabolism ; Chloroplasts ; Fluorescence ; Hydrophobic and Hydrophilic Interactions ; Kinetics ; Life Sciences ; Light ; Manganese - metabolism ; Mitochondria ; Molecular Docking Simulation ; Original Article ; Oxygen - metabolism ; Photosynthesis ; Photosynthetic apparatus ; Photosystem II ; Photosystem II Protein Complex - metabolism ; Plant Genetics and Genomics ; Plant Physiology ; Plant Sciences ; Plastoquinone - analogs & derivatives ; Plastoquinone - pharmacology ; Spectroscopy</subject><ispartof>Photosynthesis research, 2019-11, Vol.142 (2), p.229-240</ispartof><rights>Springer Nature B.V. 2019</rights><rights>COPYRIGHT 2019 Springer</rights><rights>Photosynthesis Research is a copyright of Springer, (2019). All Rights Reserved.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c476t-91775a0bdffc767a2daf4449b2149c9e2b84d3d1b10b5f9cd89a87c9f2c7e12c3</citedby><cites>FETCH-LOGICAL-c476t-91775a0bdffc767a2daf4449b2149c9e2b84d3d1b10b5f9cd89a87c9f2c7e12c3</cites><orcidid>0000-0003-1268-4414</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://link.springer.com/content/pdf/10.1007/s11120-019-00657-2$$EPDF$$P50$$Gspringer$$H</linktopdf><linktohtml>$$Uhttps://link.springer.com/10.1007/s11120-019-00657-2$$EHTML$$P50$$Gspringer$$H</linktohtml><link.rule.ids>314,776,780,27901,27902,41464,42533,51294</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/31302832$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Ptushenko, Vasily V.</creatorcontrib><creatorcontrib>Solovchenko, Alexei E.</creatorcontrib><creatorcontrib>Bychkov, Andrew Y.</creatorcontrib><creatorcontrib>Chivkunova, Olga B.</creatorcontrib><creatorcontrib>Golovin, Andrey V.</creatorcontrib><creatorcontrib>Gorelova, Olga A.</creatorcontrib><creatorcontrib>Ismagulova, Tatiana T.</creatorcontrib><creatorcontrib>Kulik, Leonid V.</creatorcontrib><creatorcontrib>Lobakova, Elena S.</creatorcontrib><creatorcontrib>Lukyanov, Alexandr A.</creatorcontrib><creatorcontrib>Samoilova, Rima I.</creatorcontrib><creatorcontrib>Scherbakov, Pavel N.</creatorcontrib><creatorcontrib>Selyakh, Irina O.</creatorcontrib><creatorcontrib>Semenova, Larisa R.</creatorcontrib><creatorcontrib>Vasilieva, Svetlana G.</creatorcontrib><creatorcontrib>Baulina, Olga I.</creatorcontrib><creatorcontrib>Skulachev, Maxim V.</creatorcontrib><creatorcontrib>Kirpichnikov, Mikhail P.</creatorcontrib><title>Cationic penetrating antioxidants switch off Mn cluster of photosystem II in situ</title><title>Photosynthesis research</title><addtitle>Photosynth Res</addtitle><addtitle>Photosynth Res</addtitle><description>Mitochondria-targeted antioxidants (also known as ‘Skulachev Ions’ electrophoretically accumulated by mitochondria) exert anti-ageing and ROS-protecting effects well documented in animal and human cells. However, their effects on chloroplast in photosynthetic cells and corresponding mechanisms are scarcely known. For the first time, we describe a dramatic quenching effect of (10-(6-plastoquinonyl)decyl triphenylphosphonium (SkQ1) on chlorophyll fluorescence, apparently mediated by redox interaction of SkQ1 with Mn cluster in Photosystem II (PSII) of chlorophyte microalga
Chlorella vulgaris
and disabling the oxygen-evolving complex (OEC). Microalgal cells displayed a vigorous uptake of SkQ1 which internal concentration built up to a very high level. Using optical and EPR spectroscopy, as well as electron donors and in silico molecular simulation techniques, we found that SkQ1 molecule can interact with Mn atoms of the OEC in PSII. This stops water splitting giving rise to potent quencher(s), e.g. oxidized reaction centre of PSII. Other components of the photosynthetic apparatus proved to be mostly intact. This effect of the Skulachev ions might help to develop in vivo models of photosynthetic cells with impaired OEC function but essentially intact otherwise. The observed phenomenon suggests that SkQ1 can be applied to study stress-induced damages to OEC in photosynthetic organisms.</description><subject>Aging</subject><subject>Antioxidants</subject><subject>Antioxidants - metabolism</subject><subject>Biochemistry</subject><subject>Biomedical and Life Sciences</subject><subject>Cations</subject><subject>Cells</subject><subject>Chlorella vulgaris - drug effects</subject><subject>Chlorella vulgaris - metabolism</subject><subject>Chlorophyll</subject><subject>Chlorophyll - metabolism</subject><subject>Chloroplasts</subject><subject>Fluorescence</subject><subject>Hydrophobic and Hydrophilic Interactions</subject><subject>Kinetics</subject><subject>Life Sciences</subject><subject>Light</subject><subject>Manganese - metabolism</subject><subject>Mitochondria</subject><subject>Molecular Docking Simulation</subject><subject>Original Article</subject><subject>Oxygen - metabolism</subject><subject>Photosynthesis</subject><subject>Photosynthetic apparatus</subject><subject>Photosystem II</subject><subject>Photosystem II Protein Complex - metabolism</subject><subject>Plant Genetics and Genomics</subject><subject>Plant Physiology</subject><subject>Plant Sciences</subject><subject>Plastoquinone - analogs & derivatives</subject><subject>Plastoquinone - pharmacology</subject><subject>Spectroscopy</subject><issn>0166-8595</issn><issn>1573-5079</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2019</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>BENPR</sourceid><recordid>eNp9kU1v1DAQhi0EosvCH-CALHGhh7RjJ47jY7WCslIrVD7OluPYW1eJvdiO2v57vGxptQghH0YzfubVzLwIvSVwQgD4aSKEUKiAiAqgZbyiz9CCMF5XDLh4jhZA2rbqmGBH6FVKNwDQtaR-iY5qUgPtarpAVyuVXfBO463xJseS-Q1WvhTv3FBiwunWZX2Ng7X40mM9zimbWFK8vQ45pPuSTni9xs7j5PL8Gr2wakzmzUNcoh-fPn5ffa4uvpyvV2cXlW54mytBOGcK-sFazVuu6KBs0zSip6QRWhjad81QD6Qn0DMr9NAJ1XEtLNXcEKrrJfqw193G8HM2KcvJJW3GUXkT5iQpZR1hHYi6oO__Qm_CHH2Zbkdx4IR29InaqNFI520o59A7UXnWAmONgHK9JTr5B1XeYCangzfWlfpBw_FBQ2GyucsbNack19--HrJ0z-oYUorGym10k4r3koDceS73nsviufztudzN_e5hu7mfzPDY8sfkAtR7IJUvvzHxaf3_yP4CySG0LA</recordid><startdate>20191101</startdate><enddate>20191101</enddate><creator>Ptushenko, Vasily V.</creator><creator>Solovchenko, Alexei E.</creator><creator>Bychkov, Andrew Y.</creator><creator>Chivkunova, Olga B.</creator><creator>Golovin, Andrey V.</creator><creator>Gorelova, Olga A.</creator><creator>Ismagulova, Tatiana T.</creator><creator>Kulik, Leonid V.</creator><creator>Lobakova, Elena S.</creator><creator>Lukyanov, Alexandr A.</creator><creator>Samoilova, Rima I.</creator><creator>Scherbakov, Pavel N.</creator><creator>Selyakh, Irina O.</creator><creator>Semenova, Larisa R.</creator><creator>Vasilieva, Svetlana G.</creator><creator>Baulina, Olga I.</creator><creator>Skulachev, Maxim V.</creator><creator>Kirpichnikov, Mikhail P.</creator><general>Springer Netherlands</general><general>Springer</general><general>Springer Nature B.V</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>ISR</scope><scope>3V.</scope><scope>7QP</scope><scope>7X7</scope><scope>7XB</scope><scope>88A</scope><scope>88E</scope><scope>88I</scope><scope>8AO</scope><scope>8FE</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BHPHI</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>LK8</scope><scope>M0S</scope><scope>M1P</scope><scope>M2P</scope><scope>M7N</scope><scope>M7P</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>Q9U</scope><scope>7X8</scope><orcidid>https://orcid.org/0000-0003-1268-4414</orcidid></search><sort><creationdate>20191101</creationdate><title>Cationic penetrating antioxidants switch off Mn cluster of photosystem II in situ</title><author>Ptushenko, Vasily V. ; Solovchenko, Alexei E. ; Bychkov, Andrew Y. ; Chivkunova, Olga B. ; Golovin, Andrey V. ; Gorelova, Olga A. ; Ismagulova, Tatiana T. ; Kulik, Leonid V. ; Lobakova, Elena S. ; Lukyanov, Alexandr A. ; Samoilova, Rima I. ; Scherbakov, Pavel N. ; Selyakh, Irina O. ; Semenova, Larisa R. ; Vasilieva, Svetlana G. ; Baulina, Olga I. ; Skulachev, Maxim V. ; Kirpichnikov, Mikhail P.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c476t-91775a0bdffc767a2daf4449b2149c9e2b84d3d1b10b5f9cd89a87c9f2c7e12c3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2019</creationdate><topic>Aging</topic><topic>Antioxidants</topic><topic>Antioxidants - metabolism</topic><topic>Biochemistry</topic><topic>Biomedical and Life Sciences</topic><topic>Cations</topic><topic>Cells</topic><topic>Chlorella vulgaris - drug effects</topic><topic>Chlorella vulgaris - metabolism</topic><topic>Chlorophyll</topic><topic>Chlorophyll - metabolism</topic><topic>Chloroplasts</topic><topic>Fluorescence</topic><topic>Hydrophobic and Hydrophilic Interactions</topic><topic>Kinetics</topic><topic>Life Sciences</topic><topic>Light</topic><topic>Manganese - metabolism</topic><topic>Mitochondria</topic><topic>Molecular Docking Simulation</topic><topic>Original Article</topic><topic>Oxygen - metabolism</topic><topic>Photosynthesis</topic><topic>Photosynthetic apparatus</topic><topic>Photosystem II</topic><topic>Photosystem II Protein Complex - metabolism</topic><topic>Plant Genetics and Genomics</topic><topic>Plant Physiology</topic><topic>Plant Sciences</topic><topic>Plastoquinone - analogs & derivatives</topic><topic>Plastoquinone - pharmacology</topic><topic>Spectroscopy</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Ptushenko, Vasily V.</creatorcontrib><creatorcontrib>Solovchenko, Alexei E.</creatorcontrib><creatorcontrib>Bychkov, Andrew Y.</creatorcontrib><creatorcontrib>Chivkunova, Olga B.</creatorcontrib><creatorcontrib>Golovin, Andrey V.</creatorcontrib><creatorcontrib>Gorelova, Olga A.</creatorcontrib><creatorcontrib>Ismagulova, Tatiana T.</creatorcontrib><creatorcontrib>Kulik, Leonid V.</creatorcontrib><creatorcontrib>Lobakova, Elena S.</creatorcontrib><creatorcontrib>Lukyanov, Alexandr A.</creatorcontrib><creatorcontrib>Samoilova, Rima I.</creatorcontrib><creatorcontrib>Scherbakov, Pavel N.</creatorcontrib><creatorcontrib>Selyakh, Irina O.</creatorcontrib><creatorcontrib>Semenova, Larisa R.</creatorcontrib><creatorcontrib>Vasilieva, Svetlana G.</creatorcontrib><creatorcontrib>Baulina, Olga I.</creatorcontrib><creatorcontrib>Skulachev, Maxim V.</creatorcontrib><creatorcontrib>Kirpichnikov, Mikhail P.</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Gale In Context: Science</collection><collection>ProQuest Central (Corporate)</collection><collection>Calcium & Calcified Tissue Abstracts</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Biology Database (Alumni Edition)</collection><collection>Medical Database (Alumni Edition)</collection><collection>Science Database (Alumni Edition)</collection><collection>ProQuest Pharma Collection</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest Central UK/Ireland</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>ProQuest Central</collection><collection>Natural Science Collection</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central Korea</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>SciTech Premium Collection</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>ProQuest Biological Science Collection</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Medical Database</collection><collection>Science Database</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>Biological Science Database</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central China</collection><collection>ProQuest Central Basic</collection><collection>MEDLINE - Academic</collection><jtitle>Photosynthesis research</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Ptushenko, Vasily V.</au><au>Solovchenko, Alexei E.</au><au>Bychkov, Andrew Y.</au><au>Chivkunova, Olga B.</au><au>Golovin, Andrey V.</au><au>Gorelova, Olga A.</au><au>Ismagulova, Tatiana T.</au><au>Kulik, Leonid V.</au><au>Lobakova, Elena S.</au><au>Lukyanov, Alexandr A.</au><au>Samoilova, Rima I.</au><au>Scherbakov, Pavel N.</au><au>Selyakh, Irina O.</au><au>Semenova, Larisa R.</au><au>Vasilieva, Svetlana G.</au><au>Baulina, Olga I.</au><au>Skulachev, Maxim V.</au><au>Kirpichnikov, Mikhail P.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Cationic penetrating antioxidants switch off Mn cluster of photosystem II in situ</atitle><jtitle>Photosynthesis research</jtitle><stitle>Photosynth Res</stitle><addtitle>Photosynth Res</addtitle><date>2019-11-01</date><risdate>2019</risdate><volume>142</volume><issue>2</issue><spage>229</spage><epage>240</epage><pages>229-240</pages><issn>0166-8595</issn><eissn>1573-5079</eissn><abstract>Mitochondria-targeted antioxidants (also known as ‘Skulachev Ions’ electrophoretically accumulated by mitochondria) exert anti-ageing and ROS-protecting effects well documented in animal and human cells. However, their effects on chloroplast in photosynthetic cells and corresponding mechanisms are scarcely known. For the first time, we describe a dramatic quenching effect of (10-(6-plastoquinonyl)decyl triphenylphosphonium (SkQ1) on chlorophyll fluorescence, apparently mediated by redox interaction of SkQ1 with Mn cluster in Photosystem II (PSII) of chlorophyte microalga
Chlorella vulgaris
and disabling the oxygen-evolving complex (OEC). Microalgal cells displayed a vigorous uptake of SkQ1 which internal concentration built up to a very high level. Using optical and EPR spectroscopy, as well as electron donors and in silico molecular simulation techniques, we found that SkQ1 molecule can interact with Mn atoms of the OEC in PSII. This stops water splitting giving rise to potent quencher(s), e.g. oxidized reaction centre of PSII. Other components of the photosynthetic apparatus proved to be mostly intact. This effect of the Skulachev ions might help to develop in vivo models of photosynthetic cells with impaired OEC function but essentially intact otherwise. The observed phenomenon suggests that SkQ1 can be applied to study stress-induced damages to OEC in photosynthetic organisms.</abstract><cop>Dordrecht</cop><pub>Springer Netherlands</pub><pmid>31302832</pmid><doi>10.1007/s11120-019-00657-2</doi><tpages>12</tpages><orcidid>https://orcid.org/0000-0003-1268-4414</orcidid></addata></record> |
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| ispartof | Photosynthesis research, 2019-11, Vol.142 (2), p.229-240 |
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| subjects | Aging Antioxidants Antioxidants - metabolism Biochemistry Biomedical and Life Sciences Cations Cells Chlorella vulgaris - drug effects Chlorella vulgaris - metabolism Chlorophyll Chlorophyll - metabolism Chloroplasts Fluorescence Hydrophobic and Hydrophilic Interactions Kinetics Life Sciences Light Manganese - metabolism Mitochondria Molecular Docking Simulation Original Article Oxygen - metabolism Photosynthesis Photosynthetic apparatus Photosystem II Photosystem II Protein Complex - metabolism Plant Genetics and Genomics Plant Physiology Plant Sciences Plastoquinone - analogs & derivatives Plastoquinone - pharmacology Spectroscopy |
| title | Cationic penetrating antioxidants switch off Mn cluster of photosystem II in situ |
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