Expression analysis of genes located within the common deleted region of del(20q) in patients with myelodysplastic syndromes
•Among 26 genes examined, expression of 8 genes was reduced in patients with del(20q).•Expression of 3 genes was reduced in patients without del(20q).•Reduced BCAS4 expression could be a prognostic marker for MDS. Deletion of the long arm of chromosome 20 (del(20q)) is observed in 5–10% of patients...
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Veröffentlicht in: | Leukemia research 2019-09, Vol.84, p.106175-106175, Article 106175 |
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creator | Shiseki, Masayuki Ishii, Mayuko Okada, Michiko Ohwashi, Mari Wang, Yan-Hua Osanai, Satoko Yoshinaga, Kentaro Mori, Naoki Motoji, Toshiko Tanaka, Junji |
description | •Among 26 genes examined, expression of 8 genes was reduced in patients with del(20q).•Expression of 3 genes was reduced in patients without del(20q).•Reduced BCAS4 expression could be a prognostic marker for MDS.
Deletion of the long arm of chromosome 20 (del(20q)) is observed in 5–10% of patients with myelodysplastic syndromes (MDS). We examined the expression of 28 genes within the common deleted region (CDR) of del(20q), which we previously determined by a CGH array using clinical samples, in 48 MDS patients with (n = 28) or without (n = 20) chromosome 20 abnormalities and control subjects (n = 10). The expression level of 8 of 28 genes was significantly reduced in MDS patients with chromosome 20 abnormalities compared to that of control subjects. In addition, the expression of BCAS4, ADA, and YWHAB genes was significantly reduced in MDS patients without chromosome 20 abnormalities, which suggests that these three genes were commonly involved in the molecular pathogenesis of MDS. To evaluate the clinical significance, we analyzed the impact of the expression level of each gene on overall survival (OS). According to the Cox proportional hazard model, multivariate analysis indicated that reduced BCAS4 expression was associated with inferior OS, but the difference was not significant (HR, 3.77; 95% CI, 0.995-17.17; P = 0.0509). Functional analyses are needed to understand the biological significance of reduced expression of these genes in the pathogenesis of MDS. |
doi_str_mv | 10.1016/j.leukres.2019.106175 |
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Deletion of the long arm of chromosome 20 (del(20q)) is observed in 5–10% of patients with myelodysplastic syndromes (MDS). We examined the expression of 28 genes within the common deleted region (CDR) of del(20q), which we previously determined by a CGH array using clinical samples, in 48 MDS patients with (n = 28) or without (n = 20) chromosome 20 abnormalities and control subjects (n = 10). The expression level of 8 of 28 genes was significantly reduced in MDS patients with chromosome 20 abnormalities compared to that of control subjects. In addition, the expression of BCAS4, ADA, and YWHAB genes was significantly reduced in MDS patients without chromosome 20 abnormalities, which suggests that these three genes were commonly involved in the molecular pathogenesis of MDS. To evaluate the clinical significance, we analyzed the impact of the expression level of each gene on overall survival (OS). According to the Cox proportional hazard model, multivariate analysis indicated that reduced BCAS4 expression was associated with inferior OS, but the difference was not significant (HR, 3.77; 95% CI, 0.995-17.17; P = 0.0509). Functional analyses are needed to understand the biological significance of reduced expression of these genes in the pathogenesis of MDS.</description><identifier>ISSN: 0145-2126</identifier><identifier>EISSN: 1873-5835</identifier><identifier>DOI: 10.1016/j.leukres.2019.106175</identifier><identifier>PMID: 31299412</identifier><language>eng</language><publisher>England: Elsevier Ltd</publisher><subject>Aged ; Aged, 80 and over ; Biomarkers ; Case-Control Studies ; Chromosome Banding ; Chromosome Deletion ; Chromosomes, Human, Pair 20 ; Common deleted region ; Deletion 20q ; Female ; Gene Expression Profiling ; Gene Expression Regulation ; Humans ; Karyotype ; Male ; Middle Aged ; Myelodysplastic syndromes ; Myelodysplastic Syndromes - diagnosis ; Myelodysplastic Syndromes - genetics ; Myelodysplastic Syndromes - therapy</subject><ispartof>Leukemia research, 2019-09, Vol.84, p.106175-106175, Article 106175</ispartof><rights>2019 Elsevier Ltd</rights><rights>Copyright © 2019 Elsevier Ltd. All rights reserved.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c365t-e00dabde9caf82db976821a000195a98eb6a069fed754b628581e5c2b602b63b3</citedby><cites>FETCH-LOGICAL-c365t-e00dabde9caf82db976821a000195a98eb6a069fed754b628581e5c2b602b63b3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/j.leukres.2019.106175$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,780,784,3550,27924,27925,45995</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/31299412$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Shiseki, Masayuki</creatorcontrib><creatorcontrib>Ishii, Mayuko</creatorcontrib><creatorcontrib>Okada, Michiko</creatorcontrib><creatorcontrib>Ohwashi, Mari</creatorcontrib><creatorcontrib>Wang, Yan-Hua</creatorcontrib><creatorcontrib>Osanai, Satoko</creatorcontrib><creatorcontrib>Yoshinaga, Kentaro</creatorcontrib><creatorcontrib>Mori, Naoki</creatorcontrib><creatorcontrib>Motoji, Toshiko</creatorcontrib><creatorcontrib>Tanaka, Junji</creatorcontrib><title>Expression analysis of genes located within the common deleted region of del(20q) in patients with myelodysplastic syndromes</title><title>Leukemia research</title><addtitle>Leuk Res</addtitle><description>•Among 26 genes examined, expression of 8 genes was reduced in patients with del(20q).•Expression of 3 genes was reduced in patients without del(20q).•Reduced BCAS4 expression could be a prognostic marker for MDS.
Deletion of the long arm of chromosome 20 (del(20q)) is observed in 5–10% of patients with myelodysplastic syndromes (MDS). We examined the expression of 28 genes within the common deleted region (CDR) of del(20q), which we previously determined by a CGH array using clinical samples, in 48 MDS patients with (n = 28) or without (n = 20) chromosome 20 abnormalities and control subjects (n = 10). The expression level of 8 of 28 genes was significantly reduced in MDS patients with chromosome 20 abnormalities compared to that of control subjects. In addition, the expression of BCAS4, ADA, and YWHAB genes was significantly reduced in MDS patients without chromosome 20 abnormalities, which suggests that these three genes were commonly involved in the molecular pathogenesis of MDS. To evaluate the clinical significance, we analyzed the impact of the expression level of each gene on overall survival (OS). According to the Cox proportional hazard model, multivariate analysis indicated that reduced BCAS4 expression was associated with inferior OS, but the difference was not significant (HR, 3.77; 95% CI, 0.995-17.17; P = 0.0509). Functional analyses are needed to understand the biological significance of reduced expression of these genes in the pathogenesis of MDS.</description><subject>Aged</subject><subject>Aged, 80 and over</subject><subject>Biomarkers</subject><subject>Case-Control Studies</subject><subject>Chromosome Banding</subject><subject>Chromosome Deletion</subject><subject>Chromosomes, Human, Pair 20</subject><subject>Common deleted region</subject><subject>Deletion 20q</subject><subject>Female</subject><subject>Gene Expression Profiling</subject><subject>Gene Expression Regulation</subject><subject>Humans</subject><subject>Karyotype</subject><subject>Male</subject><subject>Middle Aged</subject><subject>Myelodysplastic syndromes</subject><subject>Myelodysplastic Syndromes - diagnosis</subject><subject>Myelodysplastic Syndromes - genetics</subject><subject>Myelodysplastic Syndromes - therapy</subject><issn>0145-2126</issn><issn>1873-5835</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2019</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkE2PFCEQhonRuOPqT9BwXA89Aj3QzcmYzfqRbOJFz4SG6l1GuumlGLUTf7yMM3r1QEiqnrcq9RDykrMtZ1y92W8jHL5lwK1gXNea4p18RDa879pG9q18TDaM72QjuFAX5BninjEmNddPyUXLhdY7Ljbk183PpQ7BkGZqZxtXDEjTSO9gBqQxOVvA0x-h3IeZlnugLk1TZT1EOHYy3B2jNVErV4I9vKYVXGwJMBf8E6TTCjH5FZdosQRHcZ19ThPgc_JktBHhxfm_JF_f33y5_tjcfv7w6frdbeNaJUsDjHk7eNDOjr3wg-5UL7it53Atre5hUJYpPYLv5G5Qopc9B-nEoFh97dBekqvT3CWnhwNgMVNABzHaGdIBjRCy6-osKSoqT6jLCTHDaJYcJptXw5k5ijd7cxZvjuLNSXzNvTqvOAwT-H-pv6Yr8PYEQD30e4Bs0FVHDnzI4IrxKfxnxW-ilZlR</recordid><startdate>201909</startdate><enddate>201909</enddate><creator>Shiseki, Masayuki</creator><creator>Ishii, Mayuko</creator><creator>Okada, Michiko</creator><creator>Ohwashi, Mari</creator><creator>Wang, Yan-Hua</creator><creator>Osanai, Satoko</creator><creator>Yoshinaga, Kentaro</creator><creator>Mori, Naoki</creator><creator>Motoji, Toshiko</creator><creator>Tanaka, Junji</creator><general>Elsevier Ltd</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>201909</creationdate><title>Expression analysis of genes located within the common deleted region of del(20q) in patients with myelodysplastic syndromes</title><author>Shiseki, Masayuki ; Ishii, Mayuko ; Okada, Michiko ; Ohwashi, Mari ; Wang, Yan-Hua ; Osanai, Satoko ; Yoshinaga, Kentaro ; Mori, Naoki ; Motoji, Toshiko ; Tanaka, Junji</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c365t-e00dabde9caf82db976821a000195a98eb6a069fed754b628581e5c2b602b63b3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2019</creationdate><topic>Aged</topic><topic>Aged, 80 and over</topic><topic>Biomarkers</topic><topic>Case-Control Studies</topic><topic>Chromosome Banding</topic><topic>Chromosome Deletion</topic><topic>Chromosomes, Human, Pair 20</topic><topic>Common deleted region</topic><topic>Deletion 20q</topic><topic>Female</topic><topic>Gene Expression Profiling</topic><topic>Gene Expression Regulation</topic><topic>Humans</topic><topic>Karyotype</topic><topic>Male</topic><topic>Middle Aged</topic><topic>Myelodysplastic syndromes</topic><topic>Myelodysplastic Syndromes - diagnosis</topic><topic>Myelodysplastic Syndromes - genetics</topic><topic>Myelodysplastic Syndromes - therapy</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Shiseki, Masayuki</creatorcontrib><creatorcontrib>Ishii, Mayuko</creatorcontrib><creatorcontrib>Okada, Michiko</creatorcontrib><creatorcontrib>Ohwashi, Mari</creatorcontrib><creatorcontrib>Wang, Yan-Hua</creatorcontrib><creatorcontrib>Osanai, Satoko</creatorcontrib><creatorcontrib>Yoshinaga, Kentaro</creatorcontrib><creatorcontrib>Mori, Naoki</creatorcontrib><creatorcontrib>Motoji, Toshiko</creatorcontrib><creatorcontrib>Tanaka, Junji</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Leukemia research</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Shiseki, Masayuki</au><au>Ishii, Mayuko</au><au>Okada, Michiko</au><au>Ohwashi, Mari</au><au>Wang, Yan-Hua</au><au>Osanai, Satoko</au><au>Yoshinaga, Kentaro</au><au>Mori, Naoki</au><au>Motoji, Toshiko</au><au>Tanaka, Junji</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Expression analysis of genes located within the common deleted region of del(20q) in patients with myelodysplastic syndromes</atitle><jtitle>Leukemia research</jtitle><addtitle>Leuk Res</addtitle><date>2019-09</date><risdate>2019</risdate><volume>84</volume><spage>106175</spage><epage>106175</epage><pages>106175-106175</pages><artnum>106175</artnum><issn>0145-2126</issn><eissn>1873-5835</eissn><abstract>•Among 26 genes examined, expression of 8 genes was reduced in patients with del(20q).•Expression of 3 genes was reduced in patients without del(20q).•Reduced BCAS4 expression could be a prognostic marker for MDS.
Deletion of the long arm of chromosome 20 (del(20q)) is observed in 5–10% of patients with myelodysplastic syndromes (MDS). We examined the expression of 28 genes within the common deleted region (CDR) of del(20q), which we previously determined by a CGH array using clinical samples, in 48 MDS patients with (n = 28) or without (n = 20) chromosome 20 abnormalities and control subjects (n = 10). The expression level of 8 of 28 genes was significantly reduced in MDS patients with chromosome 20 abnormalities compared to that of control subjects. In addition, the expression of BCAS4, ADA, and YWHAB genes was significantly reduced in MDS patients without chromosome 20 abnormalities, which suggests that these three genes were commonly involved in the molecular pathogenesis of MDS. To evaluate the clinical significance, we analyzed the impact of the expression level of each gene on overall survival (OS). According to the Cox proportional hazard model, multivariate analysis indicated that reduced BCAS4 expression was associated with inferior OS, but the difference was not significant (HR, 3.77; 95% CI, 0.995-17.17; P = 0.0509). Functional analyses are needed to understand the biological significance of reduced expression of these genes in the pathogenesis of MDS.</abstract><cop>England</cop><pub>Elsevier Ltd</pub><pmid>31299412</pmid><doi>10.1016/j.leukres.2019.106175</doi><tpages>1</tpages></addata></record> |
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subjects | Aged Aged, 80 and over Biomarkers Case-Control Studies Chromosome Banding Chromosome Deletion Chromosomes, Human, Pair 20 Common deleted region Deletion 20q Female Gene Expression Profiling Gene Expression Regulation Humans Karyotype Male Middle Aged Myelodysplastic syndromes Myelodysplastic Syndromes - diagnosis Myelodysplastic Syndromes - genetics Myelodysplastic Syndromes - therapy |
title | Expression analysis of genes located within the common deleted region of del(20q) in patients with myelodysplastic syndromes |
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