A Novel Method of Obtaining Platelet Rich Fibrin from Rats and Quantifying Platelet Count

BACKGROUND AND OBJECTIVESRat experimentation is the first line of research by which a medical hypothesis is usually tested. Platelet Rich Fibrin (PRF) is a relatively new bio-material that has shown promise to enhance healing in the field of bone research and tissue engineering. In order to perform PRF based experiments on rats, a proper protocol of obtaining PRF from rats needs to be established. MATERIALS AND METHODS35 Wistar rats were used to obtain PRF by using cardiac puncture blood draw and quick subsequent centrifugation. The PRF samples wereanalyzed and compared to standard literature PRF composition. RESULTSPRF samples analysis showed persistent results pertaining to known PRF composition. CONCLUSIONSOur experiment has shown that our protocol of obtaining PRF is capable of providing high quality PRF from rats.