Adult vascular dysfunction in foetal growth‐restricted guinea‐pigs is associated with a neonate‐adult switching in Nos3 DNA methylation
Aim Foetal growth restriction (FGR) is associated with endothelial dysfunction and cardiovascular diseases in adult subjects. Early vascular remodelling and epigenetic changes occurring on key endothelial genes might precede this altered vascular function. Further, it has been proposed that oxidativ...
Gespeichert in:
| Veröffentlicht in: | Acta Physiologica 2019-11, Vol.227 (3), p.e13328-n/a |
|---|---|
| Hauptverfasser: | , , , , , , , , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | n/a |
|---|---|
| container_issue | 3 |
| container_start_page | e13328 |
| container_title | Acta Physiologica |
| container_volume | 227 |
| creator | Krause, Bernardo J. Peñaloza, Estefanía Candia, Alejandro Cañas, Daniel Hernández, Cherie Arenas, German A. Peralta‐Scholz, María José Valenzuela, Rodrigo García‐Herrera, Claudio Herrera, Emilio A. |
| description | Aim
Foetal growth restriction (FGR) is associated with endothelial dysfunction and cardiovascular diseases in adult subjects. Early vascular remodelling and epigenetic changes occurring on key endothelial genes might precede this altered vascular function. Further, it has been proposed that oxidative stress during development may determine some of these epigenetic modifications. To address this issue, we studied the in vivo and ex vivo vascular function and Nos3 promoter DNA methylation in arteries from eight‐month‐old guinea‐pig born from control, FGR‐treated and FGR‐NAC‐treated pregnancies.
Methods
Femoral and carotid arteries in vivo vascular function were determined by Doppler, whilst ex vivo vascular function and biomechanical properties were assessed by wire myography. Levels of eNOS mRNA and site‐specific DNA methylation in Nos3 promoter in aorta endothelial cells (AEC) were determined by qPCR and pyrosequencing respectively.
Results
FGR adult showed an increased femoral vascular resistance (P |
| doi_str_mv | 10.1111/apha.13328 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_2256103884</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>2301739226</sourcerecordid><originalsourceid>FETCH-LOGICAL-c3578-42e68ade7c0cbbbaec7cb8cdb574ec4875d4f86c50a08a8ca345ac688dc7ef1b3</originalsourceid><addsrcrecordid>eNp9kc9O3DAQh62qCBDshQeoLHFBSEv9J4nNMaJQKqGFQ3u2JhNnY5SNFzvpam99gUo8Y5-kDks5cKgvtsefvhnrR8gJZxc8rc-wbuGCSyn0B3LIVabnXPHi49uZ6QMyi_GRMcYFl5kQ--RAcq5UIS4Pye-yHruB_oSIYweB1tvYjD0OzvfU9bTxdoCOLoPfDO2fX8_BxiE4HGxNl6PrLaTa2i0jdZFCjB4dTG8bN7QUaG99n-6JgZcuMdWxdf1yUi98lPTLoqQrO7TbDqaWx2SvgS7a2et-RH7cXH-_up3f3X_9dlXezVHmSs8zYQsNtVXIsKoqsKiw0lhXucosZlrlddboAnMGTINGkFkOWGhdo7INr-QROdt518E_jelPZuUi2q6DNPIYjRB5wZnUOkvo6Tv00Y-hT9MZIRlX8lKIIlHnOwqDjzHYxqyDW0HYGs7MlJOZcjIvOSX406tyrFa2fkP_pZIAvgM2rrPb_6hM-XBb7qR_AfXso0Y</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>2301739226</pqid></control><display><type>article</type><title>Adult vascular dysfunction in foetal growth‐restricted guinea‐pigs is associated with a neonate‐adult switching in Nos3 DNA methylation</title><source>Wiley Online Library Journals Frontfile Complete</source><creator>Krause, Bernardo J. ; Peñaloza, Estefanía ; Candia, Alejandro ; Cañas, Daniel ; Hernández, Cherie ; Arenas, German A. ; Peralta‐Scholz, María José ; Valenzuela, Rodrigo ; García‐Herrera, Claudio ; Herrera, Emilio A.</creator><creatorcontrib>Krause, Bernardo J. ; Peñaloza, Estefanía ; Candia, Alejandro ; Cañas, Daniel ; Hernández, Cherie ; Arenas, German A. ; Peralta‐Scholz, María José ; Valenzuela, Rodrigo ; García‐Herrera, Claudio ; Herrera, Emilio A.</creatorcontrib><description><![CDATA[Aim
Foetal growth restriction (FGR) is associated with endothelial dysfunction and cardiovascular diseases in adult subjects. Early vascular remodelling and epigenetic changes occurring on key endothelial genes might precede this altered vascular function. Further, it has been proposed that oxidative stress during development may determine some of these epigenetic modifications. To address this issue, we studied the in vivo and ex vivo vascular function and Nos3 promoter DNA methylation in arteries from eight‐month‐old guinea‐pig born from control, FGR‐treated and FGR‐NAC‐treated pregnancies.
Methods
Femoral and carotid arteries in vivo vascular function were determined by Doppler, whilst ex vivo vascular function and biomechanical properties were assessed by wire myography. Levels of eNOS mRNA and site‐specific DNA methylation in Nos3 promoter in aorta endothelial cells (AEC) were determined by qPCR and pyrosequencing respectively.
Results
FGR adult showed an increased femoral vascular resistance (P < .05), stiffness (P < .05) and arterial remodelling (P < .01), along with an impaired NO‐mediated relaxation (P < .001). These effects were prevented by maternal treatment with NAC. Endothelial‐NOS mRNA levels were decreased in FGR adult compared with control and FGR‐NAC (P < .05), associated with increased DNA methylation levels (P < .01). Comparison of Nos3 DNA methylation in AEC showed a differential methylation pattern between foetal and adult guinea‐pigs (P < .05).
Conclusion
Altogether, these data suggest that adult vascular dysfunction in the FGR does not result from early changes in Nos3 promoter DNA methylation, but from an altered vessel structure established during foetal development.]]></description><identifier>ISSN: 1748-1708</identifier><identifier>EISSN: 1748-1716</identifier><identifier>DOI: 10.1111/apha.13328</identifier><identifier>PMID: 31177629</identifier><language>eng</language><publisher>England: Wiley Subscription Services, Inc</publisher><subject>Aorta ; Cardiovascular diseases ; Carotid artery ; Deoxyribonucleic acid ; DNA ; DNA methylation ; Endothelial cells ; endothelial function ; Epigenetics ; Femur ; foetal growth restriction ; Mechanical properties ; mRNA ; NOS3 ; Oxidative stress</subject><ispartof>Acta Physiologica, 2019-11, Vol.227 (3), p.e13328-n/a</ispartof><rights>2019 Scandinavian Physiological Society. Published by John Wiley & Sons Ltd</rights><rights>2019 Scandinavian Physiological Society. Published by John Wiley & Sons Ltd.</rights><rights>Copyright © 2019 Scandinavian Physiological Society</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c3578-42e68ade7c0cbbbaec7cb8cdb574ec4875d4f86c50a08a8ca345ac688dc7ef1b3</citedby><cites>FETCH-LOGICAL-c3578-42e68ade7c0cbbbaec7cb8cdb574ec4875d4f86c50a08a8ca345ac688dc7ef1b3</cites><orcidid>0000-0002-3563-6143</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1111%2Fapha.13328$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1111%2Fapha.13328$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>314,776,780,1411,27901,27902,45550,45551</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/31177629$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Krause, Bernardo J.</creatorcontrib><creatorcontrib>Peñaloza, Estefanía</creatorcontrib><creatorcontrib>Candia, Alejandro</creatorcontrib><creatorcontrib>Cañas, Daniel</creatorcontrib><creatorcontrib>Hernández, Cherie</creatorcontrib><creatorcontrib>Arenas, German A.</creatorcontrib><creatorcontrib>Peralta‐Scholz, María José</creatorcontrib><creatorcontrib>Valenzuela, Rodrigo</creatorcontrib><creatorcontrib>García‐Herrera, Claudio</creatorcontrib><creatorcontrib>Herrera, Emilio A.</creatorcontrib><title>Adult vascular dysfunction in foetal growth‐restricted guinea‐pigs is associated with a neonate‐adult switching in Nos3 DNA methylation</title><title>Acta Physiologica</title><addtitle>Acta Physiol (Oxf)</addtitle><description><![CDATA[Aim
Foetal growth restriction (FGR) is associated with endothelial dysfunction and cardiovascular diseases in adult subjects. Early vascular remodelling and epigenetic changes occurring on key endothelial genes might precede this altered vascular function. Further, it has been proposed that oxidative stress during development may determine some of these epigenetic modifications. To address this issue, we studied the in vivo and ex vivo vascular function and Nos3 promoter DNA methylation in arteries from eight‐month‐old guinea‐pig born from control, FGR‐treated and FGR‐NAC‐treated pregnancies.
Methods
Femoral and carotid arteries in vivo vascular function were determined by Doppler, whilst ex vivo vascular function and biomechanical properties were assessed by wire myography. Levels of eNOS mRNA and site‐specific DNA methylation in Nos3 promoter in aorta endothelial cells (AEC) were determined by qPCR and pyrosequencing respectively.
Results
FGR adult showed an increased femoral vascular resistance (P < .05), stiffness (P < .05) and arterial remodelling (P < .01), along with an impaired NO‐mediated relaxation (P < .001). These effects were prevented by maternal treatment with NAC. Endothelial‐NOS mRNA levels were decreased in FGR adult compared with control and FGR‐NAC (P < .05), associated with increased DNA methylation levels (P < .01). Comparison of Nos3 DNA methylation in AEC showed a differential methylation pattern between foetal and adult guinea‐pigs (P < .05).
Conclusion
Altogether, these data suggest that adult vascular dysfunction in the FGR does not result from early changes in Nos3 promoter DNA methylation, but from an altered vessel structure established during foetal development.]]></description><subject>Aorta</subject><subject>Cardiovascular diseases</subject><subject>Carotid artery</subject><subject>Deoxyribonucleic acid</subject><subject>DNA</subject><subject>DNA methylation</subject><subject>Endothelial cells</subject><subject>endothelial function</subject><subject>Epigenetics</subject><subject>Femur</subject><subject>foetal growth restriction</subject><subject>Mechanical properties</subject><subject>mRNA</subject><subject>NOS3</subject><subject>Oxidative stress</subject><issn>1748-1708</issn><issn>1748-1716</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2019</creationdate><recordtype>article</recordtype><recordid>eNp9kc9O3DAQh62qCBDshQeoLHFBSEv9J4nNMaJQKqGFQ3u2JhNnY5SNFzvpam99gUo8Y5-kDks5cKgvtsefvhnrR8gJZxc8rc-wbuGCSyn0B3LIVabnXPHi49uZ6QMyi_GRMcYFl5kQ--RAcq5UIS4Pye-yHruB_oSIYweB1tvYjD0OzvfU9bTxdoCOLoPfDO2fX8_BxiE4HGxNl6PrLaTa2i0jdZFCjB4dTG8bN7QUaG99n-6JgZcuMdWxdf1yUi98lPTLoqQrO7TbDqaWx2SvgS7a2et-RH7cXH-_up3f3X_9dlXezVHmSs8zYQsNtVXIsKoqsKiw0lhXucosZlrlddboAnMGTINGkFkOWGhdo7INr-QROdt518E_jelPZuUi2q6DNPIYjRB5wZnUOkvo6Tv00Y-hT9MZIRlX8lKIIlHnOwqDjzHYxqyDW0HYGs7MlJOZcjIvOSX406tyrFa2fkP_pZIAvgM2rrPb_6hM-XBb7qR_AfXso0Y</recordid><startdate>201911</startdate><enddate>201911</enddate><creator>Krause, Bernardo J.</creator><creator>Peñaloza, Estefanía</creator><creator>Candia, Alejandro</creator><creator>Cañas, Daniel</creator><creator>Hernández, Cherie</creator><creator>Arenas, German A.</creator><creator>Peralta‐Scholz, María José</creator><creator>Valenzuela, Rodrigo</creator><creator>García‐Herrera, Claudio</creator><creator>Herrera, Emilio A.</creator><general>Wiley Subscription Services, Inc</general><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7TK</scope><scope>7TS</scope><scope>7X8</scope><orcidid>https://orcid.org/0000-0002-3563-6143</orcidid></search><sort><creationdate>201911</creationdate><title>Adult vascular dysfunction in foetal growth‐restricted guinea‐pigs is associated with a neonate‐adult switching in Nos3 DNA methylation</title><author>Krause, Bernardo J. ; Peñaloza, Estefanía ; Candia, Alejandro ; Cañas, Daniel ; Hernández, Cherie ; Arenas, German A. ; Peralta‐Scholz, María José ; Valenzuela, Rodrigo ; García‐Herrera, Claudio ; Herrera, Emilio A.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c3578-42e68ade7c0cbbbaec7cb8cdb574ec4875d4f86c50a08a8ca345ac688dc7ef1b3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2019</creationdate><topic>Aorta</topic><topic>Cardiovascular diseases</topic><topic>Carotid artery</topic><topic>Deoxyribonucleic acid</topic><topic>DNA</topic><topic>DNA methylation</topic><topic>Endothelial cells</topic><topic>endothelial function</topic><topic>Epigenetics</topic><topic>Femur</topic><topic>foetal growth restriction</topic><topic>Mechanical properties</topic><topic>mRNA</topic><topic>NOS3</topic><topic>Oxidative stress</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Krause, Bernardo J.</creatorcontrib><creatorcontrib>Peñaloza, Estefanía</creatorcontrib><creatorcontrib>Candia, Alejandro</creatorcontrib><creatorcontrib>Cañas, Daniel</creatorcontrib><creatorcontrib>Hernández, Cherie</creatorcontrib><creatorcontrib>Arenas, German A.</creatorcontrib><creatorcontrib>Peralta‐Scholz, María José</creatorcontrib><creatorcontrib>Valenzuela, Rodrigo</creatorcontrib><creatorcontrib>García‐Herrera, Claudio</creatorcontrib><creatorcontrib>Herrera, Emilio A.</creatorcontrib><collection>PubMed</collection><collection>CrossRef</collection><collection>Neurosciences Abstracts</collection><collection>Physical Education Index</collection><collection>MEDLINE - Academic</collection><jtitle>Acta Physiologica</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Krause, Bernardo J.</au><au>Peñaloza, Estefanía</au><au>Candia, Alejandro</au><au>Cañas, Daniel</au><au>Hernández, Cherie</au><au>Arenas, German A.</au><au>Peralta‐Scholz, María José</au><au>Valenzuela, Rodrigo</au><au>García‐Herrera, Claudio</au><au>Herrera, Emilio A.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Adult vascular dysfunction in foetal growth‐restricted guinea‐pigs is associated with a neonate‐adult switching in Nos3 DNA methylation</atitle><jtitle>Acta Physiologica</jtitle><addtitle>Acta Physiol (Oxf)</addtitle><date>2019-11</date><risdate>2019</risdate><volume>227</volume><issue>3</issue><spage>e13328</spage><epage>n/a</epage><pages>e13328-n/a</pages><issn>1748-1708</issn><eissn>1748-1716</eissn><abstract><![CDATA[Aim
Foetal growth restriction (FGR) is associated with endothelial dysfunction and cardiovascular diseases in adult subjects. Early vascular remodelling and epigenetic changes occurring on key endothelial genes might precede this altered vascular function. Further, it has been proposed that oxidative stress during development may determine some of these epigenetic modifications. To address this issue, we studied the in vivo and ex vivo vascular function and Nos3 promoter DNA methylation in arteries from eight‐month‐old guinea‐pig born from control, FGR‐treated and FGR‐NAC‐treated pregnancies.
Methods
Femoral and carotid arteries in vivo vascular function were determined by Doppler, whilst ex vivo vascular function and biomechanical properties were assessed by wire myography. Levels of eNOS mRNA and site‐specific DNA methylation in Nos3 promoter in aorta endothelial cells (AEC) were determined by qPCR and pyrosequencing respectively.
Results
FGR adult showed an increased femoral vascular resistance (P < .05), stiffness (P < .05) and arterial remodelling (P < .01), along with an impaired NO‐mediated relaxation (P < .001). These effects were prevented by maternal treatment with NAC. Endothelial‐NOS mRNA levels were decreased in FGR adult compared with control and FGR‐NAC (P < .05), associated with increased DNA methylation levels (P < .01). Comparison of Nos3 DNA methylation in AEC showed a differential methylation pattern between foetal and adult guinea‐pigs (P < .05).
Conclusion
Altogether, these data suggest that adult vascular dysfunction in the FGR does not result from early changes in Nos3 promoter DNA methylation, but from an altered vessel structure established during foetal development.]]></abstract><cop>England</cop><pub>Wiley Subscription Services, Inc</pub><pmid>31177629</pmid><doi>10.1111/apha.13328</doi><tpages>13</tpages><orcidid>https://orcid.org/0000-0002-3563-6143</orcidid></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 1748-1708 |
| ispartof | Acta Physiologica, 2019-11, Vol.227 (3), p.e13328-n/a |
| issn | 1748-1708 1748-1716 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_2256103884 |
| source | Wiley Online Library Journals Frontfile Complete |
| subjects | Aorta Cardiovascular diseases Carotid artery Deoxyribonucleic acid DNA DNA methylation Endothelial cells endothelial function Epigenetics Femur foetal growth restriction Mechanical properties mRNA NOS3 Oxidative stress |
| title | Adult vascular dysfunction in foetal growth‐restricted guinea‐pigs is associated with a neonate‐adult switching in Nos3 DNA methylation |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-02-11T06%3A31%3A05IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Adult%20vascular%20dysfunction%20in%20foetal%20growth%E2%80%90restricted%20guinea%E2%80%90pigs%20is%20associated%20with%20a%20neonate%E2%80%90adult%20switching%20in%20Nos3%20DNA%20methylation&rft.jtitle=Acta%20Physiologica&rft.au=Krause,%20Bernardo%20J.&rft.date=2019-11&rft.volume=227&rft.issue=3&rft.spage=e13328&rft.epage=n/a&rft.pages=e13328-n/a&rft.issn=1748-1708&rft.eissn=1748-1716&rft_id=info:doi/10.1111/apha.13328&rft_dat=%3Cproquest_cross%3E2301739226%3C/proquest_cross%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=2301739226&rft_id=info:pmid/31177629&rfr_iscdi=true |