Overcoming genetic heterogeneity in industrial fermentations
Engineering the synthesis of massive amounts of therapeutics, enzymes or commodity chemicals can select for subpopulations of nonproducer cells, owing to metabolic burden and product toxicity. Deep DNA sequencing can be used to detect undesirable genetic heterogeneity in producer populations and dia...
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| Veröffentlicht in: | Nature biotechnology 2019-08, Vol.37 (8), p.869-876 |
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| creator | Rugbjerg, Peter Sommer, Morten O. A. |
| description | Engineering the synthesis of massive amounts of therapeutics, enzymes or commodity chemicals can select for subpopulations of nonproducer cells, owing to metabolic burden and product toxicity. Deep DNA sequencing can be used to detect undesirable genetic heterogeneity in producer populations and diagnose associated genetic error modes. Hotspots of genetic heterogeneity can pinpoint mechanisms that underlie load problems and product toxicity. Understanding genetic heterogeneity will inform metabolic engineering and synthetic biology strategies to minimize the emergence of nonproducer mutants in scaled-up fermentations and maximize product quality and yield.
Detection and mitigation of strain instability in industrial microbiology scale-up. |
| doi_str_mv | 10.1038/s41587-019-0171-6 |
| format | Article |
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Detection and mitigation of strain instability in industrial microbiology scale-up.</abstract><cop>New York</cop><pub>Nature Publishing Group US</pub><pmid>31285593</pmid><doi>10.1038/s41587-019-0171-6</doi><tpages>8</tpages><orcidid>https://orcid.org/0000-0003-4005-5674</orcidid><orcidid>https://orcid.org/0000-0003-2561-5063</orcidid></addata></record> |
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| subjects | 631/181/457 631/326/252 631/61/318 631/61/514/2254 Agriculture Bioinformatics Biomedical and Life Sciences Biomedical Engineering/Biotechnology Biomedicine Biotechnology Deoxyribonucleic acid DNA DNA sequencing Error detection Heterogeneity Life Sciences Metabolic engineering Metabolism Nonproducer cells Organic chemistry Perspective Population genetics Subpopulations Toxicity |
| title | Overcoming genetic heterogeneity in industrial fermentations |
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