Temperature-responsive extraction of violacein using a tuneable anionic surfactant-based system

A tuneable thermoresponsive system based on combinations of sodium dodecyl sulfate surfactant with tetrabutylammonium chloride salt is presented as an integrated process for the solid-liquid extraction of violacein from bio-engineered Yarrowia lipolytica yeast and its purification by cloud-point separation from contaminant proteins. A tuneable and thermoresponsive ionic system is applied to the extraction and cloud-point separation of violacein from biomass.