Development of a Mouse Model of Knee Osteoarthritis Based on Obesity and Bipedal Walking
ABSTRACT To investigate the influence of obesity and obligatory bipedal walking on osteoarthritis (OA) development, 26‐week‐old C57BL/6 mice were divided into two groups and obesity was induced in one group with a 60% fat diet. After 8 weeks, mice from each group were again divided into two groups a...
Gespeichert in:
| Veröffentlicht in: | Journal of orthopaedic research 2019-11, Vol.37 (11), p.2411-2419 |
|---|---|
| Hauptverfasser: | , , , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 2419 |
|---|---|
| container_issue | 11 |
| container_start_page | 2411 |
| container_title | Journal of orthopaedic research |
| container_volume | 37 |
| creator | Son, Kyeong Min Jung, Hyun A. Hong, Jeong Im Park, In Young Kim, Hyun Ah |
| description | ABSTRACT
To investigate the influence of obesity and obligatory bipedal walking on osteoarthritis (OA) development, 26‐week‐old C57BL/6 mice were divided into two groups and obesity was induced in one group with a 60% fat diet. After 8 weeks, mice from each group were again divided into two groups and obligatory bipedal exercise was induced with a specially designed treadmill in one group, resulting in four experimental groups (control, control bipedal, obese, and obese bipedal). After 8, 10, and 12 weeks of bipedal walking, knee joints were obtained and graded. Surface fibrillation and matrix proteoglycan depletion, began to appear after 8 weeks of exercise in the bipedal groups and progressed as the duration of the exercise increased. At 12 weeks, cartilage loss extending >75% of articular cartilage was observed in none of the control and obese groups, and in 42.8% and 77.7% of control bipedal and obese bipedal animals, respectively. OA grading was significantly higher in the obese bipedal group compared with the control bipedal group. The von Frey fiber test thresholds decreased significantly in the bipedal groups compared with the control and obese groups. This model can be used to study the pathogenesis of human OA and to evaluate its therapeutic agents. © 2019 Orthopaedic Research Society. Published by Wiley Periodicals, Inc. J Orthop Res 37:2411–2419, 2019 |
| doi_str_mv | 10.1002/jor.24411 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_2253830987</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>2253830987</sourcerecordid><originalsourceid>FETCH-LOGICAL-c3601-d344b97b1affc52c7c1800737474d62a9395147cc9238e45c8f3126d48369cb23</originalsourceid><addsrcrecordid>eNp1kMtOwzAURC0EoqWw4AeQl7BI61fiZEnLm6JICER3kePcQEpe2Cmof49LCjs2d6Sro9HMIHRMyZgSwibLxoyZEJTuoCH1feH5TC520ZBIHniEBcEAHVi7JIRIysJ9NOBOOBF0iBYX8All01ZQd7jJscIPzcqCuxmUm8d9DYBj20GjTPdmiq6weKosZLipcZyCLbo1VnWGp0ULmSrxiyrfi_r1EO3lqrRwtNURer66fJrdePP4-nZ2Pvc0Dwj1Mi5EGsmUqjzXPtNS09DF5FJIkQVMRTzyqZBaR4yHIHwd5i58kImQB5FOGR-h0963Nc3HCmyXVIXVUJaqBtckYcznrmsUSoee9ag2jbUG8qQ1RaXMOqEk2QyZuCGTnyEde7K1XaUVZH_k73IOmPTAV1HC-n-n5C5-7C2_ARj-ewE</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>2253830987</pqid></control><display><type>article</type><title>Development of a Mouse Model of Knee Osteoarthritis Based on Obesity and Bipedal Walking</title><source>MEDLINE</source><source>Wiley Online Library Journals Frontfile Complete</source><source>Wiley Free Content</source><creator>Son, Kyeong Min ; Jung, Hyun A. ; Hong, Jeong Im ; Park, In Young ; Kim, Hyun Ah</creator><creatorcontrib>Son, Kyeong Min ; Jung, Hyun A. ; Hong, Jeong Im ; Park, In Young ; Kim, Hyun Ah</creatorcontrib><description>ABSTRACT
To investigate the influence of obesity and obligatory bipedal walking on osteoarthritis (OA) development, 26‐week‐old C57BL/6 mice were divided into two groups and obesity was induced in one group with a 60% fat diet. After 8 weeks, mice from each group were again divided into two groups and obligatory bipedal exercise was induced with a specially designed treadmill in one group, resulting in four experimental groups (control, control bipedal, obese, and obese bipedal). After 8, 10, and 12 weeks of bipedal walking, knee joints were obtained and graded. Surface fibrillation and matrix proteoglycan depletion, began to appear after 8 weeks of exercise in the bipedal groups and progressed as the duration of the exercise increased. At 12 weeks, cartilage loss extending >75% of articular cartilage was observed in none of the control and obese groups, and in 42.8% and 77.7% of control bipedal and obese bipedal animals, respectively. OA grading was significantly higher in the obese bipedal group compared with the control bipedal group. The von Frey fiber test thresholds decreased significantly in the bipedal groups compared with the control and obese groups. This model can be used to study the pathogenesis of human OA and to evaluate its therapeutic agents. © 2019 Orthopaedic Research Society. Published by Wiley Periodicals, Inc. J Orthop Res 37:2411–2419, 2019</description><identifier>ISSN: 0736-0266</identifier><identifier>EISSN: 1554-527X</identifier><identifier>DOI: 10.1002/jor.24411</identifier><identifier>PMID: 31283041</identifier><language>eng</language><publisher>United States</publisher><subject>Animals ; bipedal ; Disease Models, Animal ; Hyperalgesia ; Joints - pathology ; Male ; Mice, Inbred C57BL ; mouse ; obesity ; Obesity - complications ; osteoarthritis ; Osteoarthritis, Knee - etiology ; Osteoarthritis, Knee - pathology ; treadmill running ; Walking</subject><ispartof>Journal of orthopaedic research, 2019-11, Vol.37 (11), p.2411-2419</ispartof><rights>2019 Orthopaedic Research Society. Published by Wiley Periodicals, Inc.</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c3601-d344b97b1affc52c7c1800737474d62a9395147cc9238e45c8f3126d48369cb23</citedby><cites>FETCH-LOGICAL-c3601-d344b97b1affc52c7c1800737474d62a9395147cc9238e45c8f3126d48369cb23</cites><orcidid>0000-0002-7948-9624</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1002%2Fjor.24411$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1002%2Fjor.24411$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>314,780,784,1417,1433,27924,27925,45574,45575,46409,46833</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/31283041$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Son, Kyeong Min</creatorcontrib><creatorcontrib>Jung, Hyun A.</creatorcontrib><creatorcontrib>Hong, Jeong Im</creatorcontrib><creatorcontrib>Park, In Young</creatorcontrib><creatorcontrib>Kim, Hyun Ah</creatorcontrib><title>Development of a Mouse Model of Knee Osteoarthritis Based on Obesity and Bipedal Walking</title><title>Journal of orthopaedic research</title><addtitle>J Orthop Res</addtitle><description>ABSTRACT
To investigate the influence of obesity and obligatory bipedal walking on osteoarthritis (OA) development, 26‐week‐old C57BL/6 mice were divided into two groups and obesity was induced in one group with a 60% fat diet. After 8 weeks, mice from each group were again divided into two groups and obligatory bipedal exercise was induced with a specially designed treadmill in one group, resulting in four experimental groups (control, control bipedal, obese, and obese bipedal). After 8, 10, and 12 weeks of bipedal walking, knee joints were obtained and graded. Surface fibrillation and matrix proteoglycan depletion, began to appear after 8 weeks of exercise in the bipedal groups and progressed as the duration of the exercise increased. At 12 weeks, cartilage loss extending >75% of articular cartilage was observed in none of the control and obese groups, and in 42.8% and 77.7% of control bipedal and obese bipedal animals, respectively. OA grading was significantly higher in the obese bipedal group compared with the control bipedal group. The von Frey fiber test thresholds decreased significantly in the bipedal groups compared with the control and obese groups. This model can be used to study the pathogenesis of human OA and to evaluate its therapeutic agents. © 2019 Orthopaedic Research Society. Published by Wiley Periodicals, Inc. J Orthop Res 37:2411–2419, 2019</description><subject>Animals</subject><subject>bipedal</subject><subject>Disease Models, Animal</subject><subject>Hyperalgesia</subject><subject>Joints - pathology</subject><subject>Male</subject><subject>Mice, Inbred C57BL</subject><subject>mouse</subject><subject>obesity</subject><subject>Obesity - complications</subject><subject>osteoarthritis</subject><subject>Osteoarthritis, Knee - etiology</subject><subject>Osteoarthritis, Knee - pathology</subject><subject>treadmill running</subject><subject>Walking</subject><issn>0736-0266</issn><issn>1554-527X</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2019</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp1kMtOwzAURC0EoqWw4AeQl7BI61fiZEnLm6JICER3kePcQEpe2Cmof49LCjs2d6Sro9HMIHRMyZgSwibLxoyZEJTuoCH1feH5TC520ZBIHniEBcEAHVi7JIRIysJ9NOBOOBF0iBYX8All01ZQd7jJscIPzcqCuxmUm8d9DYBj20GjTPdmiq6weKosZLipcZyCLbo1VnWGp0ULmSrxiyrfi_r1EO3lqrRwtNURer66fJrdePP4-nZ2Pvc0Dwj1Mi5EGsmUqjzXPtNS09DF5FJIkQVMRTzyqZBaR4yHIHwd5i58kImQB5FOGR-h0963Nc3HCmyXVIXVUJaqBtckYcznrmsUSoee9ag2jbUG8qQ1RaXMOqEk2QyZuCGTnyEde7K1XaUVZH_k73IOmPTAV1HC-n-n5C5-7C2_ARj-ewE</recordid><startdate>201911</startdate><enddate>201911</enddate><creator>Son, Kyeong Min</creator><creator>Jung, Hyun A.</creator><creator>Hong, Jeong Im</creator><creator>Park, In Young</creator><creator>Kim, Hyun Ah</creator><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><orcidid>https://orcid.org/0000-0002-7948-9624</orcidid></search><sort><creationdate>201911</creationdate><title>Development of a Mouse Model of Knee Osteoarthritis Based on Obesity and Bipedal Walking</title><author>Son, Kyeong Min ; Jung, Hyun A. ; Hong, Jeong Im ; Park, In Young ; Kim, Hyun Ah</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c3601-d344b97b1affc52c7c1800737474d62a9395147cc9238e45c8f3126d48369cb23</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2019</creationdate><topic>Animals</topic><topic>bipedal</topic><topic>Disease Models, Animal</topic><topic>Hyperalgesia</topic><topic>Joints - pathology</topic><topic>Male</topic><topic>Mice, Inbred C57BL</topic><topic>mouse</topic><topic>obesity</topic><topic>Obesity - complications</topic><topic>osteoarthritis</topic><topic>Osteoarthritis, Knee - etiology</topic><topic>Osteoarthritis, Knee - pathology</topic><topic>treadmill running</topic><topic>Walking</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Son, Kyeong Min</creatorcontrib><creatorcontrib>Jung, Hyun A.</creatorcontrib><creatorcontrib>Hong, Jeong Im</creatorcontrib><creatorcontrib>Park, In Young</creatorcontrib><creatorcontrib>Kim, Hyun Ah</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Journal of orthopaedic research</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Son, Kyeong Min</au><au>Jung, Hyun A.</au><au>Hong, Jeong Im</au><au>Park, In Young</au><au>Kim, Hyun Ah</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Development of a Mouse Model of Knee Osteoarthritis Based on Obesity and Bipedal Walking</atitle><jtitle>Journal of orthopaedic research</jtitle><addtitle>J Orthop Res</addtitle><date>2019-11</date><risdate>2019</risdate><volume>37</volume><issue>11</issue><spage>2411</spage><epage>2419</epage><pages>2411-2419</pages><issn>0736-0266</issn><eissn>1554-527X</eissn><abstract>ABSTRACT
To investigate the influence of obesity and obligatory bipedal walking on osteoarthritis (OA) development, 26‐week‐old C57BL/6 mice were divided into two groups and obesity was induced in one group with a 60% fat diet. After 8 weeks, mice from each group were again divided into two groups and obligatory bipedal exercise was induced with a specially designed treadmill in one group, resulting in four experimental groups (control, control bipedal, obese, and obese bipedal). After 8, 10, and 12 weeks of bipedal walking, knee joints were obtained and graded. Surface fibrillation and matrix proteoglycan depletion, began to appear after 8 weeks of exercise in the bipedal groups and progressed as the duration of the exercise increased. At 12 weeks, cartilage loss extending >75% of articular cartilage was observed in none of the control and obese groups, and in 42.8% and 77.7% of control bipedal and obese bipedal animals, respectively. OA grading was significantly higher in the obese bipedal group compared with the control bipedal group. The von Frey fiber test thresholds decreased significantly in the bipedal groups compared with the control and obese groups. This model can be used to study the pathogenesis of human OA and to evaluate its therapeutic agents. © 2019 Orthopaedic Research Society. Published by Wiley Periodicals, Inc. J Orthop Res 37:2411–2419, 2019</abstract><cop>United States</cop><pmid>31283041</pmid><doi>10.1002/jor.24411</doi><tpages>9</tpages><orcidid>https://orcid.org/0000-0002-7948-9624</orcidid><oa>free_for_read</oa></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0736-0266 |
| ispartof | Journal of orthopaedic research, 2019-11, Vol.37 (11), p.2411-2419 |
| issn | 0736-0266 1554-527X |
| language | eng |
| recordid | cdi_proquest_miscellaneous_2253830987 |
| source | MEDLINE; Wiley Online Library Journals Frontfile Complete; Wiley Free Content |
| subjects | Animals bipedal Disease Models, Animal Hyperalgesia Joints - pathology Male Mice, Inbred C57BL mouse obesity Obesity - complications osteoarthritis Osteoarthritis, Knee - etiology Osteoarthritis, Knee - pathology treadmill running Walking |
| title | Development of a Mouse Model of Knee Osteoarthritis Based on Obesity and Bipedal Walking |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-06T20%3A06%3A28IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Development%20of%20a%20Mouse%20Model%20of%20Knee%20Osteoarthritis%20Based%20on%20Obesity%20and%20Bipedal%20Walking&rft.jtitle=Journal%20of%20orthopaedic%20research&rft.au=Son,%20Kyeong%20Min&rft.date=2019-11&rft.volume=37&rft.issue=11&rft.spage=2411&rft.epage=2419&rft.pages=2411-2419&rft.issn=0736-0266&rft.eissn=1554-527X&rft_id=info:doi/10.1002/jor.24411&rft_dat=%3Cproquest_cross%3E2253830987%3C/proquest_cross%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=2253830987&rft_id=info:pmid/31283041&rfr_iscdi=true |