PRDM14 and BLIMP1 control the development of chicken primordial germ cells
The differentiation of primordial germ cells (PGCs) is a fundamental step in development. PR domain-containing protein 14 (PRDM14) and B lymphocyte-induced maturation protein 1 (BLIMP1) play pivotal roles in mouse PGC specification. In the present study, we assessed the roles of chicken orthologs of...
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| Veröffentlicht in: | Developmental biology 2019-11, Vol.455 (1), p.32-41 |
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| creator | Okuzaki, Yuya Kaneoka, Hidenori Suzuki, Takayuki Hagihara, Yota Nakayama, Yuki Murakami, Seitaro Murase, Yusuke Kuroiwa, Atsushi Iijima, Shinji Nishijima, Ken-ichi |
| description | The differentiation of primordial germ cells (PGCs) is a fundamental step in development. PR domain-containing protein 14 (PRDM14) and B lymphocyte-induced maturation protein 1 (BLIMP1) play pivotal roles in mouse PGC specification. In the present study, we assessed the roles of chicken orthologs of PRDM14 and BLIMP1 in PGC development. PRDM14 and BLIMP1 were expressed in blastodermal cells and PGCs. The in vivo knockdown of PRDM14 or BLIMP1 by introducing a replication-competent retroviral vector expressing shRNAs to the blastodermal stage of embryos reduced the number of SSEA-1 or chicken vasa homologue-positive PGCs on day 5.5–6.5. Since the inhibition of Activin receptor-like kinase 4/5/7 in cultured PGCs reduced the expression of PRDM14, BLIMP1, and NANOG, and that of MEK inhibited PRDM14 expression, the expression of these genes seems to be controlled by Activin A and FGF2 signaling. Overall, PRDM14, BLIMP1, and NANOG seem to be involved in the self-renewal of PGCs in cultured PGCs and embryos.
[Display omitted]
•Chicken PRDM14 and BLIMP1 are needed for proper development of primordial germ cells in vivo.•Knocking down BLIMP1 reduces PRDM14 in cultured primordial germ cells.•BLIMP1 and PRDM14 regulate NANOG expression in primordial germ cells.•The in vivo knockdown of PRDM14 or BLIMP1 reduced the number of PGCs on day 5.5–6.5. |
| doi_str_mv | 10.1016/j.ydbio.2019.06.018 |
| format | Article |
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[Display omitted]
•Chicken PRDM14 and BLIMP1 are needed for proper development of primordial germ cells in vivo.•Knocking down BLIMP1 reduces PRDM14 in cultured primordial germ cells.•BLIMP1 and PRDM14 regulate NANOG expression in primordial germ cells.•The in vivo knockdown of PRDM14 or BLIMP1 reduced the number of PGCs on day 5.5–6.5.</description><identifier>ISSN: 0012-1606</identifier><identifier>EISSN: 1095-564X</identifier><identifier>DOI: 10.1016/j.ydbio.2019.06.018</identifier><identifier>PMID: 31271752</identifier><language>eng</language><publisher>United States: Elsevier Inc</publisher><subject>Animals ; Avian Proteins - genetics ; Avian Proteins - metabolism ; Blastoderm - cytology ; Blastoderm - metabolism ; BLIMP1 ; Cell Differentiation - genetics ; Cell Self Renewal - genetics ; Cells, Cultured ; Chick Embryo ; Chicken ; Chickens ; CVH ; Gene Expression Regulation, Developmental ; Germ Cells - cytology ; Germ Cells - metabolism ; Lewis X Antigen - genetics ; Lewis X Antigen - metabolism ; Nanog Homeobox Protein - genetics ; Nanog Homeobox Protein - metabolism ; Positive Regulatory Domain I-Binding Factor 1 - genetics ; Positive Regulatory Domain I-Binding Factor 1 - metabolism ; PRDM14 ; Primordial germ cells ; RNA Interference</subject><ispartof>Developmental biology, 2019-11, Vol.455 (1), p.32-41</ispartof><rights>2019 Elsevier Inc.</rights><rights>Copyright © 2019 Elsevier Inc. All rights reserved.</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c514t-e75b17335090e3169f7b0a03028f534a60656cb6ad828db0be632c783089e38e3</citedby><cites>FETCH-LOGICAL-c514t-e75b17335090e3169f7b0a03028f534a60656cb6ad828db0be632c783089e38e3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://www.sciencedirect.com/science/article/pii/S0012160618307991$$EHTML$$P50$$Gelsevier$$Hfree_for_read</linktohtml><link.rule.ids>314,776,780,3537,27901,27902,65306</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/31271752$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Okuzaki, Yuya</creatorcontrib><creatorcontrib>Kaneoka, Hidenori</creatorcontrib><creatorcontrib>Suzuki, Takayuki</creatorcontrib><creatorcontrib>Hagihara, Yota</creatorcontrib><creatorcontrib>Nakayama, Yuki</creatorcontrib><creatorcontrib>Murakami, Seitaro</creatorcontrib><creatorcontrib>Murase, Yusuke</creatorcontrib><creatorcontrib>Kuroiwa, Atsushi</creatorcontrib><creatorcontrib>Iijima, Shinji</creatorcontrib><creatorcontrib>Nishijima, Ken-ichi</creatorcontrib><title>PRDM14 and BLIMP1 control the development of chicken primordial germ cells</title><title>Developmental biology</title><addtitle>Dev Biol</addtitle><description>The differentiation of primordial germ cells (PGCs) is a fundamental step in development. PR domain-containing protein 14 (PRDM14) and B lymphocyte-induced maturation protein 1 (BLIMP1) play pivotal roles in mouse PGC specification. In the present study, we assessed the roles of chicken orthologs of PRDM14 and BLIMP1 in PGC development. PRDM14 and BLIMP1 were expressed in blastodermal cells and PGCs. The in vivo knockdown of PRDM14 or BLIMP1 by introducing a replication-competent retroviral vector expressing shRNAs to the blastodermal stage of embryos reduced the number of SSEA-1 or chicken vasa homologue-positive PGCs on day 5.5–6.5. Since the inhibition of Activin receptor-like kinase 4/5/7 in cultured PGCs reduced the expression of PRDM14, BLIMP1, and NANOG, and that of MEK inhibited PRDM14 expression, the expression of these genes seems to be controlled by Activin A and FGF2 signaling. Overall, PRDM14, BLIMP1, and NANOG seem to be involved in the self-renewal of PGCs in cultured PGCs and embryos.
[Display omitted]
•Chicken PRDM14 and BLIMP1 are needed for proper development of primordial germ cells in vivo.•Knocking down BLIMP1 reduces PRDM14 in cultured primordial germ cells.•BLIMP1 and PRDM14 regulate NANOG expression in primordial germ cells.•The in vivo knockdown of PRDM14 or BLIMP1 reduced the number of PGCs on day 5.5–6.5.</description><subject>Animals</subject><subject>Avian Proteins - genetics</subject><subject>Avian Proteins - metabolism</subject><subject>Blastoderm - cytology</subject><subject>Blastoderm - metabolism</subject><subject>BLIMP1</subject><subject>Cell Differentiation - genetics</subject><subject>Cell Self Renewal - genetics</subject><subject>Cells, Cultured</subject><subject>Chick Embryo</subject><subject>Chicken</subject><subject>Chickens</subject><subject>CVH</subject><subject>Gene Expression Regulation, Developmental</subject><subject>Germ Cells - cytology</subject><subject>Germ Cells - metabolism</subject><subject>Lewis X Antigen - genetics</subject><subject>Lewis X Antigen - metabolism</subject><subject>Nanog Homeobox Protein - genetics</subject><subject>Nanog Homeobox Protein - metabolism</subject><subject>Positive Regulatory Domain I-Binding Factor 1 - genetics</subject><subject>Positive Regulatory Domain I-Binding Factor 1 - metabolism</subject><subject>PRDM14</subject><subject>Primordial germ cells</subject><subject>RNA Interference</subject><issn>0012-1606</issn><issn>1095-564X</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2019</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9kE1v1DAQhq2Kim6X_oJKyEcuCTP22nEOHKBQKNqKCoHUm5XYE-olibd2tlL_PVm2cOxpLu_XPIydI5QIqN9uykffhlgKwLoEXQKaI7ZAqFWh9Or2BVsAoChQgz5hpzlvAEAaI1-yE4miwkqJBft68_3jNa54M3r-YX11fYPcxXFKsefTHXFPD9TH7UDjxGPH3V1wv2nk2xSGmHxoev6L0sAd9X1-xY67ps909nSX7Oflpx8XX4r1t89XF-_XhVO4mgqqVIuVlApqIIm67qoWGpAgTKfkqpnnKu1a3XgjjG-hJS2Fq4wEU5M0JJfszSF3m-L9jvJkh5D3C5qR4i5bIZSUCHq2LJk8SF2KOSfq7H55kx4tgt1DtBv7F6LdQ7Sg7Qxxdr1-Kti1A_n_nn_UZsG7g4DmNx8CJZtdoNGRD4ncZH0Mzxb8AcPAgVM</recordid><startdate>20191101</startdate><enddate>20191101</enddate><creator>Okuzaki, Yuya</creator><creator>Kaneoka, Hidenori</creator><creator>Suzuki, Takayuki</creator><creator>Hagihara, Yota</creator><creator>Nakayama, Yuki</creator><creator>Murakami, Seitaro</creator><creator>Murase, Yusuke</creator><creator>Kuroiwa, Atsushi</creator><creator>Iijima, Shinji</creator><creator>Nishijima, Ken-ichi</creator><general>Elsevier Inc</general><scope>6I.</scope><scope>AAFTH</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>20191101</creationdate><title>PRDM14 and BLIMP1 control the development of chicken primordial germ cells</title><author>Okuzaki, Yuya ; Kaneoka, Hidenori ; Suzuki, Takayuki ; Hagihara, Yota ; Nakayama, Yuki ; Murakami, Seitaro ; Murase, Yusuke ; Kuroiwa, Atsushi ; Iijima, Shinji ; Nishijima, Ken-ichi</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c514t-e75b17335090e3169f7b0a03028f534a60656cb6ad828db0be632c783089e38e3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2019</creationdate><topic>Animals</topic><topic>Avian Proteins - genetics</topic><topic>Avian Proteins - metabolism</topic><topic>Blastoderm - cytology</topic><topic>Blastoderm - metabolism</topic><topic>BLIMP1</topic><topic>Cell Differentiation - genetics</topic><topic>Cell Self Renewal - genetics</topic><topic>Cells, Cultured</topic><topic>Chick Embryo</topic><topic>Chicken</topic><topic>Chickens</topic><topic>CVH</topic><topic>Gene Expression Regulation, Developmental</topic><topic>Germ Cells - cytology</topic><topic>Germ Cells - metabolism</topic><topic>Lewis X Antigen - genetics</topic><topic>Lewis X Antigen - metabolism</topic><topic>Nanog Homeobox Protein - genetics</topic><topic>Nanog Homeobox Protein - metabolism</topic><topic>Positive Regulatory Domain I-Binding Factor 1 - genetics</topic><topic>Positive Regulatory Domain I-Binding Factor 1 - metabolism</topic><topic>PRDM14</topic><topic>Primordial germ cells</topic><topic>RNA Interference</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Okuzaki, Yuya</creatorcontrib><creatorcontrib>Kaneoka, Hidenori</creatorcontrib><creatorcontrib>Suzuki, Takayuki</creatorcontrib><creatorcontrib>Hagihara, Yota</creatorcontrib><creatorcontrib>Nakayama, Yuki</creatorcontrib><creatorcontrib>Murakami, Seitaro</creatorcontrib><creatorcontrib>Murase, Yusuke</creatorcontrib><creatorcontrib>Kuroiwa, Atsushi</creatorcontrib><creatorcontrib>Iijima, Shinji</creatorcontrib><creatorcontrib>Nishijima, Ken-ichi</creatorcontrib><collection>ScienceDirect Open Access Titles</collection><collection>Elsevier:ScienceDirect:Open Access</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Developmental biology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Okuzaki, Yuya</au><au>Kaneoka, Hidenori</au><au>Suzuki, Takayuki</au><au>Hagihara, Yota</au><au>Nakayama, Yuki</au><au>Murakami, Seitaro</au><au>Murase, Yusuke</au><au>Kuroiwa, Atsushi</au><au>Iijima, Shinji</au><au>Nishijima, Ken-ichi</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>PRDM14 and BLIMP1 control the development of chicken primordial germ cells</atitle><jtitle>Developmental biology</jtitle><addtitle>Dev Biol</addtitle><date>2019-11-01</date><risdate>2019</risdate><volume>455</volume><issue>1</issue><spage>32</spage><epage>41</epage><pages>32-41</pages><issn>0012-1606</issn><eissn>1095-564X</eissn><abstract>The differentiation of primordial germ cells (PGCs) is a fundamental step in development. PR domain-containing protein 14 (PRDM14) and B lymphocyte-induced maturation protein 1 (BLIMP1) play pivotal roles in mouse PGC specification. In the present study, we assessed the roles of chicken orthologs of PRDM14 and BLIMP1 in PGC development. PRDM14 and BLIMP1 were expressed in blastodermal cells and PGCs. The in vivo knockdown of PRDM14 or BLIMP1 by introducing a replication-competent retroviral vector expressing shRNAs to the blastodermal stage of embryos reduced the number of SSEA-1 or chicken vasa homologue-positive PGCs on day 5.5–6.5. Since the inhibition of Activin receptor-like kinase 4/5/7 in cultured PGCs reduced the expression of PRDM14, BLIMP1, and NANOG, and that of MEK inhibited PRDM14 expression, the expression of these genes seems to be controlled by Activin A and FGF2 signaling. Overall, PRDM14, BLIMP1, and NANOG seem to be involved in the self-renewal of PGCs in cultured PGCs and embryos.
[Display omitted]
•Chicken PRDM14 and BLIMP1 are needed for proper development of primordial germ cells in vivo.•Knocking down BLIMP1 reduces PRDM14 in cultured primordial germ cells.•BLIMP1 and PRDM14 regulate NANOG expression in primordial germ cells.•The in vivo knockdown of PRDM14 or BLIMP1 reduced the number of PGCs on day 5.5–6.5.</abstract><cop>United States</cop><pub>Elsevier Inc</pub><pmid>31271752</pmid><doi>10.1016/j.ydbio.2019.06.018</doi><tpages>10</tpages><oa>free_for_read</oa></addata></record> |
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| subjects | Animals Avian Proteins - genetics Avian Proteins - metabolism Blastoderm - cytology Blastoderm - metabolism BLIMP1 Cell Differentiation - genetics Cell Self Renewal - genetics Cells, Cultured Chick Embryo Chicken Chickens CVH Gene Expression Regulation, Developmental Germ Cells - cytology Germ Cells - metabolism Lewis X Antigen - genetics Lewis X Antigen - metabolism Nanog Homeobox Protein - genetics Nanog Homeobox Protein - metabolism Positive Regulatory Domain I-Binding Factor 1 - genetics Positive Regulatory Domain I-Binding Factor 1 - metabolism PRDM14 Primordial germ cells RNA Interference |
| title | PRDM14 and BLIMP1 control the development of chicken primordial germ cells |
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