Immobilization of lipase from Pseudomonas fluorescens on glyoxyl-octyl-agarose beads: Improved stability and reusability

The lipase from Pseudomonas fluorescens (PFL) has been immobilized on glyoxyl-octyl agarose and compared to the enzyme immobilized on octyl-agarose. Thus, PFL was immobilized at pH 7 on glyoxyl-octyl support via lipase interfacial activation and later incubated at pH 10.5 for 20 h before reduction t...

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Veröffentlicht in:	Biochimica et biophysica acta. Proteins and proteomics 2019-09, Vol.1867 (9), p.741-747
Hauptverfasser:	Rios, Nathalia S., Mendez-Sanchez, Carmen, Arana-Peña, Sara, Rueda, Nazzoly, Ortiz, Claudia, Gonçalves, Luciana R.B., Fernandez-Lafuente, Roberto
Format:	Artikel
Sprache:	eng
Schlagworte:	Covalent immobilization Enzyme release Heterofunctional supports Lipase interfacial activation Triacetin hydrolysis
Online-Zugang:	Volltext
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