Effect of a novel glass ionomer cement containing fluoro-zinc-silicate fillers on biofilm formation and dentin ion incorporation
Objectives This study is aimed at evaluating the effect of a new glass ionomer cement (GIC) containing fluoro-zinc-silicate fillers on biofilm formation and ion incorporation. Materials and methods Streptococcus mutans biofilms were developed on two GIC materials: Caredyne Restore (CD) and Fuji VII...
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| Veröffentlicht in: | Clinical oral investigations 2020-02, Vol.24 (2), p.963-970 |
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| creator | Hasegawa, Taisuke Takenaka, Shoji Ohsumi, Tatsuya Ida, Takako Ohshima, Hayato Terao, Yutaka Naksagoon, Traithawit Maeda, Takeyasu Noiri, Yuichiro |
| description | Objectives
This study is aimed at evaluating the effect of a new glass ionomer cement (GIC) containing fluoro-zinc-silicate fillers on biofilm formation and ion incorporation.
Materials and methods
Streptococcus mutans
biofilms were developed on two GIC materials: Caredyne Restore (CD) and Fuji VII (FJ); and hydroxyapatite (HA) for 24 h at 37 °C using a flow cell system. The morphological structure and bacterial viability were analyzed using a confocal laser scanning microscopy. Bacterial adhesion during the initial 2 h was also assessed by viable cell counting. To study the ion incorporation, restored cavities prepared on the root surfaces of human incisors were subjected to the elemental mapping of the zinc and fluoride ions in the GIC-dentin interface using a wavelength-dispersive X-ray spectroscopy electron probe microanalyzer.
Results
Morphological observations revealed that biofilm formation in the CD group was remarkably inhibited compared with the HA and FJ groups, exhibiting sparse, thinner biofilm clusters. The microorganisms adhering to the CD group were significantly inhibited, revealing 2.9 ± 0.4 for CD, 4.9 ± 0.2 for FJ, and 5.4 ± 0.4 log colony-forming units (CFU) for HA. The CD zinc ion incorporation depth was 72.2 ± 8.0 μm. The fluoride penetration of CD was three times deeper than that of FJ; this difference was statistically significant (
p
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| doi_str_mv | 10.1007/s00784-019-02991-0 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_2253271772</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>2253271772</sourcerecordid><originalsourceid>FETCH-LOGICAL-c441t-9b149d6eb408cd5a9a31ecb14fdb0d466a831369245303de164be6c93964702d3</originalsourceid><addsrcrecordid>eNp9kUuPFCEURonROOPoH3BhSNy4QXk1FEszGR_JJG50TSi4dJhQ0EKVia786dLdoyYu3ACXe-6B5EPoOaOvGaX6TR_LJAllhlBuDCP0AbpkUigitGYPT2dOlJnYBXrS-x2lTCotHqMLwbgWOz5dop83MYJfcY3Y4VK_Qcb77HrHqZa6QMMeFigr9rWsLpVU9jjmrbZKfqTiSU85ebcCjilnaB3XgudUR7XgWNvi1uHBrgQchiWVoxaPwdoOtZ2aT9Gj6HKHZ_f7Ffry7ubz9Qdy--n9x-u3t8RLyVZiZiZNUDBLOvmwc8YJBn5cxjDTIJVyk2BCGS53gooATMkZlDfCKKkpD-IKvTp7D61-3aCvdkndQ86uQN265XwnuGZa84G-_Ae9q1sr43dHik1KCCkGxc-Ub7X3BtEeWlpc-24Ztcd87DkfO_Kxp3wsHUMv7tXbvED4M_I7kAGIM9BHq-yh_X37P9pfNjacUA</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>2251863343</pqid></control><display><type>article</type><title>Effect of a novel glass ionomer cement containing fluoro-zinc-silicate fillers on biofilm formation and dentin ion incorporation</title><source>MEDLINE</source><source>SpringerLink Journals</source><creator>Hasegawa, Taisuke ; Takenaka, Shoji ; Ohsumi, Tatsuya ; Ida, Takako ; Ohshima, Hayato ; Terao, Yutaka ; Naksagoon, Traithawit ; Maeda, Takeyasu ; Noiri, Yuichiro</creator><creatorcontrib>Hasegawa, Taisuke ; Takenaka, Shoji ; Ohsumi, Tatsuya ; Ida, Takako ; Ohshima, Hayato ; Terao, Yutaka ; Naksagoon, Traithawit ; Maeda, Takeyasu ; Noiri, Yuichiro</creatorcontrib><description>Objectives
This study is aimed at evaluating the effect of a new glass ionomer cement (GIC) containing fluoro-zinc-silicate fillers on biofilm formation and ion incorporation.
Materials and methods
Streptococcus mutans
biofilms were developed on two GIC materials: Caredyne Restore (CD) and Fuji VII (FJ); and hydroxyapatite (HA) for 24 h at 37 °C using a flow cell system. The morphological structure and bacterial viability were analyzed using a confocal laser scanning microscopy. Bacterial adhesion during the initial 2 h was also assessed by viable cell counting. To study the ion incorporation, restored cavities prepared on the root surfaces of human incisors were subjected to the elemental mapping of the zinc and fluoride ions in the GIC-dentin interface using a wavelength-dispersive X-ray spectroscopy electron probe microanalyzer.
Results
Morphological observations revealed that biofilm formation in the CD group was remarkably inhibited compared with the HA and FJ groups, exhibiting sparse, thinner biofilm clusters. The microorganisms adhering to the CD group were significantly inhibited, revealing 2.9 ± 0.4 for CD, 4.9 ± 0.2 for FJ, and 5.4 ± 0.4 log colony-forming units (CFU) for HA. The CD zinc ion incorporation depth was 72.2 ± 8.0 μm. The fluoride penetration of CD was three times deeper than that of FJ; this difference was statistically significant (
p
< 0.05).
Conclusions
Enhanced by the incorporation of zinc and fluoride ions, the new GIC inhibited biofilm formation by interfering with bacterial adhesion.
Clinical relevance
A novel GIC comprised of fluoro-zinc-silicate fillers may improve clinical outcomes, such as root caries and minimally invasive dentistry.</description><identifier>ISSN: 1432-6981</identifier><identifier>EISSN: 1436-3771</identifier><identifier>DOI: 10.1007/s00784-019-02991-0</identifier><identifier>PMID: 31273528</identifier><language>eng</language><publisher>Berlin/Heidelberg: Springer Berlin Heidelberg</publisher><subject>Bacteria ; Biofilms ; Cement ; Confocal microscopy ; Dental caries ; Dentin ; Dentistry ; Electron probe ; Fluorides ; Glass Ionomer Cements ; Humans ; Hydroxyapatite ; Incisors ; Materials Testing ; Medicine ; Morphology ; Original Article ; Silicates ; Statistical analysis ; Streptococcus infections ; X-ray spectroscopy ; Zinc</subject><ispartof>Clinical oral investigations, 2020-02, Vol.24 (2), p.963-970</ispartof><rights>Springer-Verlag GmbH Germany, part of Springer Nature 2019</rights><rights>Clinical Oral Investigations is a copyright of Springer, (2019). All Rights Reserved.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c441t-9b149d6eb408cd5a9a31ecb14fdb0d466a831369245303de164be6c93964702d3</citedby><cites>FETCH-LOGICAL-c441t-9b149d6eb408cd5a9a31ecb14fdb0d466a831369245303de164be6c93964702d3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://link.springer.com/content/pdf/10.1007/s00784-019-02991-0$$EPDF$$P50$$Gspringer$$H</linktopdf><linktohtml>$$Uhttps://link.springer.com/10.1007/s00784-019-02991-0$$EHTML$$P50$$Gspringer$$H</linktohtml><link.rule.ids>314,776,780,27903,27904,41467,42536,51298</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/31273528$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Hasegawa, Taisuke</creatorcontrib><creatorcontrib>Takenaka, Shoji</creatorcontrib><creatorcontrib>Ohsumi, Tatsuya</creatorcontrib><creatorcontrib>Ida, Takako</creatorcontrib><creatorcontrib>Ohshima, Hayato</creatorcontrib><creatorcontrib>Terao, Yutaka</creatorcontrib><creatorcontrib>Naksagoon, Traithawit</creatorcontrib><creatorcontrib>Maeda, Takeyasu</creatorcontrib><creatorcontrib>Noiri, Yuichiro</creatorcontrib><title>Effect of a novel glass ionomer cement containing fluoro-zinc-silicate fillers on biofilm formation and dentin ion incorporation</title><title>Clinical oral investigations</title><addtitle>Clin Oral Invest</addtitle><addtitle>Clin Oral Investig</addtitle><description>Objectives
This study is aimed at evaluating the effect of a new glass ionomer cement (GIC) containing fluoro-zinc-silicate fillers on biofilm formation and ion incorporation.
Materials and methods
Streptococcus mutans
biofilms were developed on two GIC materials: Caredyne Restore (CD) and Fuji VII (FJ); and hydroxyapatite (HA) for 24 h at 37 °C using a flow cell system. The morphological structure and bacterial viability were analyzed using a confocal laser scanning microscopy. Bacterial adhesion during the initial 2 h was also assessed by viable cell counting. To study the ion incorporation, restored cavities prepared on the root surfaces of human incisors were subjected to the elemental mapping of the zinc and fluoride ions in the GIC-dentin interface using a wavelength-dispersive X-ray spectroscopy electron probe microanalyzer.
Results
Morphological observations revealed that biofilm formation in the CD group was remarkably inhibited compared with the HA and FJ groups, exhibiting sparse, thinner biofilm clusters. The microorganisms adhering to the CD group were significantly inhibited, revealing 2.9 ± 0.4 for CD, 4.9 ± 0.2 for FJ, and 5.4 ± 0.4 log colony-forming units (CFU) for HA. The CD zinc ion incorporation depth was 72.2 ± 8.0 μm. The fluoride penetration of CD was three times deeper than that of FJ; this difference was statistically significant (
p
< 0.05).
Conclusions
Enhanced by the incorporation of zinc and fluoride ions, the new GIC inhibited biofilm formation by interfering with bacterial adhesion.
Clinical relevance
A novel GIC comprised of fluoro-zinc-silicate fillers may improve clinical outcomes, such as root caries and minimally invasive dentistry.</description><subject>Bacteria</subject><subject>Biofilms</subject><subject>Cement</subject><subject>Confocal microscopy</subject><subject>Dental caries</subject><subject>Dentin</subject><subject>Dentistry</subject><subject>Electron probe</subject><subject>Fluorides</subject><subject>Glass Ionomer Cements</subject><subject>Humans</subject><subject>Hydroxyapatite</subject><subject>Incisors</subject><subject>Materials Testing</subject><subject>Medicine</subject><subject>Morphology</subject><subject>Original Article</subject><subject>Silicates</subject><subject>Statistical analysis</subject><subject>Streptococcus infections</subject><subject>X-ray spectroscopy</subject><subject>Zinc</subject><issn>1432-6981</issn><issn>1436-3771</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2020</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><sourceid>GNUQQ</sourceid><recordid>eNp9kUuPFCEURonROOPoH3BhSNy4QXk1FEszGR_JJG50TSi4dJhQ0EKVia786dLdoyYu3ACXe-6B5EPoOaOvGaX6TR_LJAllhlBuDCP0AbpkUigitGYPT2dOlJnYBXrS-x2lTCotHqMLwbgWOz5dop83MYJfcY3Y4VK_Qcb77HrHqZa6QMMeFigr9rWsLpVU9jjmrbZKfqTiSU85ebcCjilnaB3XgudUR7XgWNvi1uHBrgQchiWVoxaPwdoOtZ2aT9Gj6HKHZ_f7Ffry7ubz9Qdy--n9x-u3t8RLyVZiZiZNUDBLOvmwc8YJBn5cxjDTIJVyk2BCGS53gooATMkZlDfCKKkpD-IKvTp7D61-3aCvdkndQ86uQN265XwnuGZa84G-_Ae9q1sr43dHik1KCCkGxc-Ub7X3BtEeWlpc-24Ztcd87DkfO_Kxp3wsHUMv7tXbvED4M_I7kAGIM9BHq-yh_X37P9pfNjacUA</recordid><startdate>20200201</startdate><enddate>20200201</enddate><creator>Hasegawa, Taisuke</creator><creator>Takenaka, Shoji</creator><creator>Ohsumi, Tatsuya</creator><creator>Ida, Takako</creator><creator>Ohshima, Hayato</creator><creator>Terao, Yutaka</creator><creator>Naksagoon, Traithawit</creator><creator>Maeda, Takeyasu</creator><creator>Noiri, Yuichiro</creator><general>Springer Berlin Heidelberg</general><general>Springer Nature B.V</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7X7</scope><scope>7XB</scope><scope>88E</scope><scope>8AO</scope><scope>8C1</scope><scope>8FE</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BHPHI</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>LK8</scope><scope>M0S</scope><scope>M1P</scope><scope>M7P</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>7X8</scope></search><sort><creationdate>20200201</creationdate><title>Effect of a novel glass ionomer cement containing fluoro-zinc-silicate fillers on biofilm formation and dentin ion incorporation</title><author>Hasegawa, Taisuke ; Takenaka, Shoji ; Ohsumi, Tatsuya ; Ida, Takako ; Ohshima, Hayato ; Terao, Yutaka ; Naksagoon, Traithawit ; Maeda, Takeyasu ; Noiri, Yuichiro</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c441t-9b149d6eb408cd5a9a31ecb14fdb0d466a831369245303de164be6c93964702d3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2020</creationdate><topic>Bacteria</topic><topic>Biofilms</topic><topic>Cement</topic><topic>Confocal microscopy</topic><topic>Dental caries</topic><topic>Dentin</topic><topic>Dentistry</topic><topic>Electron probe</topic><topic>Fluorides</topic><topic>Glass Ionomer Cements</topic><topic>Humans</topic><topic>Hydroxyapatite</topic><topic>Incisors</topic><topic>Materials Testing</topic><topic>Medicine</topic><topic>Morphology</topic><topic>Original Article</topic><topic>Silicates</topic><topic>Statistical analysis</topic><topic>Streptococcus infections</topic><topic>X-ray spectroscopy</topic><topic>Zinc</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Hasegawa, Taisuke</creatorcontrib><creatorcontrib>Takenaka, Shoji</creatorcontrib><creatorcontrib>Ohsumi, Tatsuya</creatorcontrib><creatorcontrib>Ida, Takako</creatorcontrib><creatorcontrib>Ohshima, Hayato</creatorcontrib><creatorcontrib>Terao, Yutaka</creatorcontrib><creatorcontrib>Naksagoon, Traithawit</creatorcontrib><creatorcontrib>Maeda, Takeyasu</creatorcontrib><creatorcontrib>Noiri, Yuichiro</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Medical Database (Alumni Edition)</collection><collection>ProQuest Pharma Collection</collection><collection>Public Health Database</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest Central UK/Ireland</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>ProQuest Central</collection><collection>Natural Science Collection</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central Korea</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>SciTech Premium Collection</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>ProQuest Biological Science Collection</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Medical Database</collection><collection>Biological Science Database</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central China</collection><collection>MEDLINE - Academic</collection><jtitle>Clinical oral investigations</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Hasegawa, Taisuke</au><au>Takenaka, Shoji</au><au>Ohsumi, Tatsuya</au><au>Ida, Takako</au><au>Ohshima, Hayato</au><au>Terao, Yutaka</au><au>Naksagoon, Traithawit</au><au>Maeda, Takeyasu</au><au>Noiri, Yuichiro</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Effect of a novel glass ionomer cement containing fluoro-zinc-silicate fillers on biofilm formation and dentin ion incorporation</atitle><jtitle>Clinical oral investigations</jtitle><stitle>Clin Oral Invest</stitle><addtitle>Clin Oral Investig</addtitle><date>2020-02-01</date><risdate>2020</risdate><volume>24</volume><issue>2</issue><spage>963</spage><epage>970</epage><pages>963-970</pages><issn>1432-6981</issn><eissn>1436-3771</eissn><abstract>Objectives
This study is aimed at evaluating the effect of a new glass ionomer cement (GIC) containing fluoro-zinc-silicate fillers on biofilm formation and ion incorporation.
Materials and methods
Streptococcus mutans
biofilms were developed on two GIC materials: Caredyne Restore (CD) and Fuji VII (FJ); and hydroxyapatite (HA) for 24 h at 37 °C using a flow cell system. The morphological structure and bacterial viability were analyzed using a confocal laser scanning microscopy. Bacterial adhesion during the initial 2 h was also assessed by viable cell counting. To study the ion incorporation, restored cavities prepared on the root surfaces of human incisors were subjected to the elemental mapping of the zinc and fluoride ions in the GIC-dentin interface using a wavelength-dispersive X-ray spectroscopy electron probe microanalyzer.
Results
Morphological observations revealed that biofilm formation in the CD group was remarkably inhibited compared with the HA and FJ groups, exhibiting sparse, thinner biofilm clusters. The microorganisms adhering to the CD group were significantly inhibited, revealing 2.9 ± 0.4 for CD, 4.9 ± 0.2 for FJ, and 5.4 ± 0.4 log colony-forming units (CFU) for HA. The CD zinc ion incorporation depth was 72.2 ± 8.0 μm. The fluoride penetration of CD was three times deeper than that of FJ; this difference was statistically significant (
p
< 0.05).
Conclusions
Enhanced by the incorporation of zinc and fluoride ions, the new GIC inhibited biofilm formation by interfering with bacterial adhesion.
Clinical relevance
A novel GIC comprised of fluoro-zinc-silicate fillers may improve clinical outcomes, such as root caries and minimally invasive dentistry.</abstract><cop>Berlin/Heidelberg</cop><pub>Springer Berlin Heidelberg</pub><pmid>31273528</pmid><doi>10.1007/s00784-019-02991-0</doi><tpages>8</tpages></addata></record> |
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| ispartof | Clinical oral investigations, 2020-02, Vol.24 (2), p.963-970 |
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| subjects | Bacteria Biofilms Cement Confocal microscopy Dental caries Dentin Dentistry Electron probe Fluorides Glass Ionomer Cements Humans Hydroxyapatite Incisors Materials Testing Medicine Morphology Original Article Silicates Statistical analysis Streptococcus infections X-ray spectroscopy Zinc |
| title | Effect of a novel glass ionomer cement containing fluoro-zinc-silicate fillers on biofilm formation and dentin ion incorporation |
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