The chondroprotective effect of diosmin on human articular chondrocytes under oxidative stress
Excessive oxidative stress, which can amplify inflammatory responses, is involved in the pathologic progression of knee osteoarthritis. Diosmin is known to possess a variety of biological functions such as antiinflammatory and antioxidant activities. We therefore demonstrated the chondroprotective p...
Gespeichert in:
| Veröffentlicht in: | Phytotherapy research 2019-09, Vol.33 (9), p.2378-2386 |
|---|---|
| Hauptverfasser: | , , , , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 2386 |
|---|---|
| container_issue | 9 |
| container_start_page | 2378 |
| container_title | Phytotherapy research |
| container_volume | 33 |
| creator | Chen, Yi‐Ru Yang, Kai‐Chiang Lu, Dai‐Hua Wu, Wen‐Tien Wang, Chen‐Chie Tsai, Mong‐Hsun |
| description | Excessive oxidative stress, which can amplify inflammatory responses, is involved in the pathologic progression of knee osteoarthritis. Diosmin is known to possess a variety of biological functions such as antiinflammatory and antioxidant activities. We therefore demonstrated the chondroprotective potentials of diosmin on human articular chondrocytes under oxidative stress. The cytotoxicity of diosmin (5, 10, 50, and 100 μM) to chondrocytes was first evaluated. Subsequently, the cells were treated with diosmin (5 and 10 μM) after hydrogen peroxide (H2O2) exposure. We found that the cytotoxicity of diosmin occurred in a dose‐dependent manner (10, 50, and 100 μM), and low‐dose diosmin (5 μM) slightly impaired cell viability. Diosmin supplementations (5 and 10 μM) did not show beneficial effects on mitochondrial activity, cytotoxicity, proliferation, and survival and the cell senescence was ameliorated in H2O2‐exposed chondrocytes. On the other hand, diosmin down‐regulated the mRNA levels of iNOS, COX‐2, IL‐1β, COL1A1, MMP‐3, and MMP‐9; up‐regulated TIMP‐1 and SOX9; and improved COL2A1 in chondrocytes under oxidative stresses. Furthermore, diosmin also regulated glutathione reductase and glutathione peroxidase of H2O2‐exposed chondrocytes. In conclusion, diosmin displayed a remarkable antiinflammatory effect compared with the antioxidant capacity on human chondrocytes. Diosmin can maintain the homeostasis of extracellular matrix of articular cartilage. |
| doi_str_mv | 10.1002/ptr.6425 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_2252269844</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>2284429255</sourcerecordid><originalsourceid>FETCH-LOGICAL-c3495-3bcc2d9893516c0641cb92910b2f209806175d0385777966189288185f5bd90b3</originalsourceid><addsrcrecordid>eNp1kMtKxDAUQIMozvgAv0ACbtx0vEmbNFnK4AsGFBnBlaFNU6ZD24xJq87fm3kpCK5yF-cebg5CZwRGBIBeLTo34glle2hIQMqIsDTeR0OQjEQJEa8DdOT9HAAkheQQDWJCUxCCD9HbdGawntm2cHbhbGd0V30YbMoyTNiWuKisb6oW2xbP-iZrcea6Svd15nZretkZj_u2MA7br6rI1gbfOeP9CToos9qb0-17jF5ub6bj-2jyePcwvp5EOk4ki-Jca1pIIWNGuAaeEJ1LKgnktKQgBXCSsgJiwdI0lZwTIakQRLCS5YWEPD5Glxtv-MN7b3ynmsprU9dZa2zvFaWMUi5FkgT04g86t71rw3WBCgCVlLFfoXbWe2dKtXBVk7mlIqBWzVVorlbNA3q-FfZ5Y4ofcBc5ANEG-Kxqs_xXpJ6mz2vhN75pieA</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>2284429255</pqid></control><display><type>article</type><title>The chondroprotective effect of diosmin on human articular chondrocytes under oxidative stress</title><source>MEDLINE</source><source>Wiley Online Library Journals Frontfile Complete</source><creator>Chen, Yi‐Ru ; Yang, Kai‐Chiang ; Lu, Dai‐Hua ; Wu, Wen‐Tien ; Wang, Chen‐Chie ; Tsai, Mong‐Hsun</creator><creatorcontrib>Chen, Yi‐Ru ; Yang, Kai‐Chiang ; Lu, Dai‐Hua ; Wu, Wen‐Tien ; Wang, Chen‐Chie ; Tsai, Mong‐Hsun</creatorcontrib><description>Excessive oxidative stress, which can amplify inflammatory responses, is involved in the pathologic progression of knee osteoarthritis. Diosmin is known to possess a variety of biological functions such as antiinflammatory and antioxidant activities. We therefore demonstrated the chondroprotective potentials of diosmin on human articular chondrocytes under oxidative stress. The cytotoxicity of diosmin (5, 10, 50, and 100 μM) to chondrocytes was first evaluated. Subsequently, the cells were treated with diosmin (5 and 10 μM) after hydrogen peroxide (H2O2) exposure. We found that the cytotoxicity of diosmin occurred in a dose‐dependent manner (10, 50, and 100 μM), and low‐dose diosmin (5 μM) slightly impaired cell viability. Diosmin supplementations (5 and 10 μM) did not show beneficial effects on mitochondrial activity, cytotoxicity, proliferation, and survival and the cell senescence was ameliorated in H2O2‐exposed chondrocytes. On the other hand, diosmin down‐regulated the mRNA levels of iNOS, COX‐2, IL‐1β, COL1A1, MMP‐3, and MMP‐9; up‐regulated TIMP‐1 and SOX9; and improved COL2A1 in chondrocytes under oxidative stresses. Furthermore, diosmin also regulated glutathione reductase and glutathione peroxidase of H2O2‐exposed chondrocytes. In conclusion, diosmin displayed a remarkable antiinflammatory effect compared with the antioxidant capacity on human chondrocytes. Diosmin can maintain the homeostasis of extracellular matrix of articular cartilage.</description><identifier>ISSN: 0951-418X</identifier><identifier>EISSN: 1099-1573</identifier><identifier>DOI: 10.1002/ptr.6425</identifier><identifier>PMID: 31270886</identifier><language>eng</language><publisher>England: Wiley Subscription Services, Inc</publisher><subject>Aged ; Antioxidants ; articular cartilage ; Biomedical materials ; Cartilage ; Cartilage (articular) ; Cartilage diseases ; Cartilage, Articular - drug effects ; Cell Survival ; Cell viability ; Chondrocytes ; Chondrocytes - drug effects ; Collagen (type I) ; Cytotoxicity ; diosmin ; Diosmin - pharmacology ; Diosmin - therapeutic use ; Exposure ; Extracellular matrix ; Glutathione ; Glutathione peroxidase ; Glutathione reductase ; Homeostasis ; Humans ; Hydrogen peroxide ; Inflammation ; Knee ; Matrix metalloproteinases ; Middle Aged ; Mitochondria ; mRNA ; Nitric-oxide synthase ; Osteoarthritis ; Osteoarthritis, Knee - drug therapy ; Oxidative stress ; Oxidative Stress - drug effects ; Peroxidase ; Reductases ; Senescence ; Tissue inhibitor of metalloproteinases ; Toxicity ; Vitamin E</subject><ispartof>Phytotherapy research, 2019-09, Vol.33 (9), p.2378-2386</ispartof><rights>2019 John Wiley & Sons, Ltd.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c3495-3bcc2d9893516c0641cb92910b2f209806175d0385777966189288185f5bd90b3</citedby><cites>FETCH-LOGICAL-c3495-3bcc2d9893516c0641cb92910b2f209806175d0385777966189288185f5bd90b3</cites><orcidid>0000-0002-0979-4463 ; 0000-0002-1694-7579</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1002%2Fptr.6425$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1002%2Fptr.6425$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>314,776,780,1411,27901,27902,45550,45551</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/31270886$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Chen, Yi‐Ru</creatorcontrib><creatorcontrib>Yang, Kai‐Chiang</creatorcontrib><creatorcontrib>Lu, Dai‐Hua</creatorcontrib><creatorcontrib>Wu, Wen‐Tien</creatorcontrib><creatorcontrib>Wang, Chen‐Chie</creatorcontrib><creatorcontrib>Tsai, Mong‐Hsun</creatorcontrib><title>The chondroprotective effect of diosmin on human articular chondrocytes under oxidative stress</title><title>Phytotherapy research</title><addtitle>Phytother Res</addtitle><description>Excessive oxidative stress, which can amplify inflammatory responses, is involved in the pathologic progression of knee osteoarthritis. Diosmin is known to possess a variety of biological functions such as antiinflammatory and antioxidant activities. We therefore demonstrated the chondroprotective potentials of diosmin on human articular chondrocytes under oxidative stress. The cytotoxicity of diosmin (5, 10, 50, and 100 μM) to chondrocytes was first evaluated. Subsequently, the cells were treated with diosmin (5 and 10 μM) after hydrogen peroxide (H2O2) exposure. We found that the cytotoxicity of diosmin occurred in a dose‐dependent manner (10, 50, and 100 μM), and low‐dose diosmin (5 μM) slightly impaired cell viability. Diosmin supplementations (5 and 10 μM) did not show beneficial effects on mitochondrial activity, cytotoxicity, proliferation, and survival and the cell senescence was ameliorated in H2O2‐exposed chondrocytes. On the other hand, diosmin down‐regulated the mRNA levels of iNOS, COX‐2, IL‐1β, COL1A1, MMP‐3, and MMP‐9; up‐regulated TIMP‐1 and SOX9; and improved COL2A1 in chondrocytes under oxidative stresses. Furthermore, diosmin also regulated glutathione reductase and glutathione peroxidase of H2O2‐exposed chondrocytes. In conclusion, diosmin displayed a remarkable antiinflammatory effect compared with the antioxidant capacity on human chondrocytes. Diosmin can maintain the homeostasis of extracellular matrix of articular cartilage.</description><subject>Aged</subject><subject>Antioxidants</subject><subject>articular cartilage</subject><subject>Biomedical materials</subject><subject>Cartilage</subject><subject>Cartilage (articular)</subject><subject>Cartilage diseases</subject><subject>Cartilage, Articular - drug effects</subject><subject>Cell Survival</subject><subject>Cell viability</subject><subject>Chondrocytes</subject><subject>Chondrocytes - drug effects</subject><subject>Collagen (type I)</subject><subject>Cytotoxicity</subject><subject>diosmin</subject><subject>Diosmin - pharmacology</subject><subject>Diosmin - therapeutic use</subject><subject>Exposure</subject><subject>Extracellular matrix</subject><subject>Glutathione</subject><subject>Glutathione peroxidase</subject><subject>Glutathione reductase</subject><subject>Homeostasis</subject><subject>Humans</subject><subject>Hydrogen peroxide</subject><subject>Inflammation</subject><subject>Knee</subject><subject>Matrix metalloproteinases</subject><subject>Middle Aged</subject><subject>Mitochondria</subject><subject>mRNA</subject><subject>Nitric-oxide synthase</subject><subject>Osteoarthritis</subject><subject>Osteoarthritis, Knee - drug therapy</subject><subject>Oxidative stress</subject><subject>Oxidative Stress - drug effects</subject><subject>Peroxidase</subject><subject>Reductases</subject><subject>Senescence</subject><subject>Tissue inhibitor of metalloproteinases</subject><subject>Toxicity</subject><subject>Vitamin E</subject><issn>0951-418X</issn><issn>1099-1573</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2019</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp1kMtKxDAUQIMozvgAv0ACbtx0vEmbNFnK4AsGFBnBlaFNU6ZD24xJq87fm3kpCK5yF-cebg5CZwRGBIBeLTo34glle2hIQMqIsDTeR0OQjEQJEa8DdOT9HAAkheQQDWJCUxCCD9HbdGawntm2cHbhbGd0V30YbMoyTNiWuKisb6oW2xbP-iZrcea6Svd15nZretkZj_u2MA7br6rI1gbfOeP9CToos9qb0-17jF5ub6bj-2jyePcwvp5EOk4ki-Jca1pIIWNGuAaeEJ1LKgnktKQgBXCSsgJiwdI0lZwTIakQRLCS5YWEPD5Glxtv-MN7b3ynmsprU9dZa2zvFaWMUi5FkgT04g86t71rw3WBCgCVlLFfoXbWe2dKtXBVk7mlIqBWzVVorlbNA3q-FfZ5Y4ofcBc5ANEG-Kxqs_xXpJ6mz2vhN75pieA</recordid><startdate>201909</startdate><enddate>201909</enddate><creator>Chen, Yi‐Ru</creator><creator>Yang, Kai‐Chiang</creator><creator>Lu, Dai‐Hua</creator><creator>Wu, Wen‐Tien</creator><creator>Wang, Chen‐Chie</creator><creator>Tsai, Mong‐Hsun</creator><general>Wiley Subscription Services, Inc</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QO</scope><scope>7QP</scope><scope>7QR</scope><scope>7TK</scope><scope>7TM</scope><scope>8FD</scope><scope>FR3</scope><scope>K9.</scope><scope>P64</scope><scope>RC3</scope><scope>7X8</scope><orcidid>https://orcid.org/0000-0002-0979-4463</orcidid><orcidid>https://orcid.org/0000-0002-1694-7579</orcidid></search><sort><creationdate>201909</creationdate><title>The chondroprotective effect of diosmin on human articular chondrocytes under oxidative stress</title><author>Chen, Yi‐Ru ; Yang, Kai‐Chiang ; Lu, Dai‐Hua ; Wu, Wen‐Tien ; Wang, Chen‐Chie ; Tsai, Mong‐Hsun</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c3495-3bcc2d9893516c0641cb92910b2f209806175d0385777966189288185f5bd90b3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2019</creationdate><topic>Aged</topic><topic>Antioxidants</topic><topic>articular cartilage</topic><topic>Biomedical materials</topic><topic>Cartilage</topic><topic>Cartilage (articular)</topic><topic>Cartilage diseases</topic><topic>Cartilage, Articular - drug effects</topic><topic>Cell Survival</topic><topic>Cell viability</topic><topic>Chondrocytes</topic><topic>Chondrocytes - drug effects</topic><topic>Collagen (type I)</topic><topic>Cytotoxicity</topic><topic>diosmin</topic><topic>Diosmin - pharmacology</topic><topic>Diosmin - therapeutic use</topic><topic>Exposure</topic><topic>Extracellular matrix</topic><topic>Glutathione</topic><topic>Glutathione peroxidase</topic><topic>Glutathione reductase</topic><topic>Homeostasis</topic><topic>Humans</topic><topic>Hydrogen peroxide</topic><topic>Inflammation</topic><topic>Knee</topic><topic>Matrix metalloproteinases</topic><topic>Middle Aged</topic><topic>Mitochondria</topic><topic>mRNA</topic><topic>Nitric-oxide synthase</topic><topic>Osteoarthritis</topic><topic>Osteoarthritis, Knee - drug therapy</topic><topic>Oxidative stress</topic><topic>Oxidative Stress - drug effects</topic><topic>Peroxidase</topic><topic>Reductases</topic><topic>Senescence</topic><topic>Tissue inhibitor of metalloproteinases</topic><topic>Toxicity</topic><topic>Vitamin E</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Chen, Yi‐Ru</creatorcontrib><creatorcontrib>Yang, Kai‐Chiang</creatorcontrib><creatorcontrib>Lu, Dai‐Hua</creatorcontrib><creatorcontrib>Wu, Wen‐Tien</creatorcontrib><creatorcontrib>Wang, Chen‐Chie</creatorcontrib><creatorcontrib>Tsai, Mong‐Hsun</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Biotechnology Research Abstracts</collection><collection>Calcium & Calcified Tissue Abstracts</collection><collection>Chemoreception Abstracts</collection><collection>Neurosciences Abstracts</collection><collection>Nucleic Acids Abstracts</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Genetics Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Phytotherapy research</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Chen, Yi‐Ru</au><au>Yang, Kai‐Chiang</au><au>Lu, Dai‐Hua</au><au>Wu, Wen‐Tien</au><au>Wang, Chen‐Chie</au><au>Tsai, Mong‐Hsun</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>The chondroprotective effect of diosmin on human articular chondrocytes under oxidative stress</atitle><jtitle>Phytotherapy research</jtitle><addtitle>Phytother Res</addtitle><date>2019-09</date><risdate>2019</risdate><volume>33</volume><issue>9</issue><spage>2378</spage><epage>2386</epage><pages>2378-2386</pages><issn>0951-418X</issn><eissn>1099-1573</eissn><abstract>Excessive oxidative stress, which can amplify inflammatory responses, is involved in the pathologic progression of knee osteoarthritis. Diosmin is known to possess a variety of biological functions such as antiinflammatory and antioxidant activities. We therefore demonstrated the chondroprotective potentials of diosmin on human articular chondrocytes under oxidative stress. The cytotoxicity of diosmin (5, 10, 50, and 100 μM) to chondrocytes was first evaluated. Subsequently, the cells were treated with diosmin (5 and 10 μM) after hydrogen peroxide (H2O2) exposure. We found that the cytotoxicity of diosmin occurred in a dose‐dependent manner (10, 50, and 100 μM), and low‐dose diosmin (5 μM) slightly impaired cell viability. Diosmin supplementations (5 and 10 μM) did not show beneficial effects on mitochondrial activity, cytotoxicity, proliferation, and survival and the cell senescence was ameliorated in H2O2‐exposed chondrocytes. On the other hand, diosmin down‐regulated the mRNA levels of iNOS, COX‐2, IL‐1β, COL1A1, MMP‐3, and MMP‐9; up‐regulated TIMP‐1 and SOX9; and improved COL2A1 in chondrocytes under oxidative stresses. Furthermore, diosmin also regulated glutathione reductase and glutathione peroxidase of H2O2‐exposed chondrocytes. In conclusion, diosmin displayed a remarkable antiinflammatory effect compared with the antioxidant capacity on human chondrocytes. Diosmin can maintain the homeostasis of extracellular matrix of articular cartilage.</abstract><cop>England</cop><pub>Wiley Subscription Services, Inc</pub><pmid>31270886</pmid><doi>10.1002/ptr.6425</doi><tpages>9</tpages><orcidid>https://orcid.org/0000-0002-0979-4463</orcidid><orcidid>https://orcid.org/0000-0002-1694-7579</orcidid></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0951-418X |
| ispartof | Phytotherapy research, 2019-09, Vol.33 (9), p.2378-2386 |
| issn | 0951-418X 1099-1573 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_2252269844 |
| source | MEDLINE; Wiley Online Library Journals Frontfile Complete |
| subjects | Aged Antioxidants articular cartilage Biomedical materials Cartilage Cartilage (articular) Cartilage diseases Cartilage, Articular - drug effects Cell Survival Cell viability Chondrocytes Chondrocytes - drug effects Collagen (type I) Cytotoxicity diosmin Diosmin - pharmacology Diosmin - therapeutic use Exposure Extracellular matrix Glutathione Glutathione peroxidase Glutathione reductase Homeostasis Humans Hydrogen peroxide Inflammation Knee Matrix metalloproteinases Middle Aged Mitochondria mRNA Nitric-oxide synthase Osteoarthritis Osteoarthritis, Knee - drug therapy Oxidative stress Oxidative Stress - drug effects Peroxidase Reductases Senescence Tissue inhibitor of metalloproteinases Toxicity Vitamin E |
| title | The chondroprotective effect of diosmin on human articular chondrocytes under oxidative stress |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-02-03T01%3A43%3A36IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=The%20chondroprotective%20effect%20of%20diosmin%20on%20human%20articular%20chondrocytes%20under%20oxidative%20stress&rft.jtitle=Phytotherapy%20research&rft.au=Chen,%20Yi%E2%80%90Ru&rft.date=2019-09&rft.volume=33&rft.issue=9&rft.spage=2378&rft.epage=2386&rft.pages=2378-2386&rft.issn=0951-418X&rft.eissn=1099-1573&rft_id=info:doi/10.1002/ptr.6425&rft_dat=%3Cproquest_cross%3E2284429255%3C/proquest_cross%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=2284429255&rft_id=info:pmid/31270886&rfr_iscdi=true |