Reduction of IL‐2 fragmentation during manufacturing of a novel immunocytokine by DoE process optimization

Interleukin‐2 (IL‐2) is a potent molecule in cancer therapy. Clinical application, however, is limited due to its strong side effects during the treatment. We developed an IL‐2 variant (IL‐2v) immunocytokine to circumvent the drawbacks of the current IL‐2 therapy. During the production of the IL‐2v...

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Veröffentlicht in:	Biotechnology and bioengineering 2019-10, Vol.116 (10), p.2503-2513
Hauptverfasser:	Schneider, Alina, Gorr, Ingo H., Larraillet, Vincent, Frensing, Timo, Popp, Oliver
Format:	Artikel
Sprache:	eng
Schlagworte:	Cell density CHO cells Design of experiments Design optimization DoE Fermentation Fragmentation Inoculation Interleukins interleukin‐2 Menopause pH effects proteolytic cleavage Side effects Therapy
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