Chemical characterization of leaves and calli extracts of Rosmarinus officinalis by UHPLC‐MS
Rosmarinus officinalis L. (Lamiaceae) is an aromatic plant widely popular mainly due to its uses in traditional medicine as an anti‐inflammatory, diuretic and antimicrobial, as well as in the prevention and treatment of diseases. These biological activities are mainly related to the presence of phen...
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| Veröffentlicht in: | Electrophoresis 2020-10, Vol.41 (20), p.1776-1783 |
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| creator | Pérez‐Mendoza, Martha Berenice Llorens‐Escobar, Laura Vanegas‐Espinoza, Pablo Emilio Cifuentes, Alejandro Ibáñez, Elena Villar‐Martínez, Alma Angélica Del |
| description | Rosmarinus officinalis L. (Lamiaceae) is an aromatic plant widely popular mainly due to its uses in traditional medicine as an anti‐inflammatory, diuretic and antimicrobial, as well as in the prevention and treatment of diseases. These biological activities are mainly related to the presence of phenolic and terpenic compounds. This work reports a chemical profile analysis of extracts from leaves and calli of rosemary obtained by both pressurized liquid extraction and maceration. Chemical profiles were determined on calli extracts of 3, 6, 9, and 15 days of culture; chemical characterization and quantification of compounds was carried out using ultrahigh performance liquid chromatography‐mass spectrometry. A total of 53 metabolites were identified in callus and 47 compounds in leaf extracts, of which 25 correspond to phenolic compounds, mainly flavonoids and flavones, 13 terpenes that include phenolic terpenes and one diterpenolactone, two glycosides which correspond to 6‐O‐caffeoyl‐β‐D‐fructofuranosil‐(2→1)‐α‐D‐glucopyranoside and primulaverin, an aromatic compound identified as fenantrenone and a growth regulator 12‐hydroxy jasmonic acid. These results showed that undifferentiated rosemary cells accumulate the same compounds identified mainly in highly specialized tissues such as leaves. The plant cell culture supply the possibility of developing biotechnological processes to obtain compounds of commercial interest. |
| doi_str_mv | 10.1002/elps.201900152 |
| format | Article |
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(Lamiaceae) is an aromatic plant widely popular mainly due to its uses in traditional medicine as an anti‐inflammatory, diuretic and antimicrobial, as well as in the prevention and treatment of diseases. These biological activities are mainly related to the presence of phenolic and terpenic compounds. This work reports a chemical profile analysis of extracts from leaves and calli of rosemary obtained by both pressurized liquid extraction and maceration. Chemical profiles were determined on calli extracts of 3, 6, 9, and 15 days of culture; chemical characterization and quantification of compounds was carried out using ultrahigh performance liquid chromatography‐mass spectrometry. A total of 53 metabolites were identified in callus and 47 compounds in leaf extracts, of which 25 correspond to phenolic compounds, mainly flavonoids and flavones, 13 terpenes that include phenolic terpenes and one diterpenolactone, two glycosides which correspond to 6‐O‐caffeoyl‐β‐D‐fructofuranosil‐(2→1)‐α‐D‐glucopyranoside and primulaverin, an aromatic compound identified as fenantrenone and a growth regulator 12‐hydroxy jasmonic acid. These results showed that undifferentiated rosemary cells accumulate the same compounds identified mainly in highly specialized tissues such as leaves. The plant cell culture supply the possibility of developing biotechnological processes to obtain compounds of commercial interest.</description><identifier>ISSN: 0173-0835</identifier><identifier>EISSN: 1522-2683</identifier><identifier>DOI: 10.1002/elps.201900152</identifier><identifier>PMID: 31267584</identifier><language>eng</language><publisher>Germany: Wiley Subscription Services, Inc</publisher><subject>Antiinfectives and antibacterials ; Aromatic compounds ; Callus ; Carnosic acid ; Carnosol ; Chromatography, High Pressure Liquid - methods ; Cinnamates - analysis ; Cinnamates - chemistry ; Depsides - analysis ; Depsides - chemistry ; Diuretics ; Flavonoids ; Flavonoids - analysis ; Flavonoids - chemistry ; Glycosides - analysis ; Glycosides - chemistry ; Growth regulators ; Jasmonic acid ; Liquid chromatography ; Mass spectrometry ; Mass Spectrometry - methods ; Metabolites ; Phenols ; Plant Extracts - chemistry ; Plant Leaves - chemistry ; Pressurized liquid extraction ; Rosemary ; Rosmarinic Acid ; Rosmarinus - chemistry ; Terpenes ; Terpenes - analysis ; Terpenes - chemistry</subject><ispartof>Electrophoresis, 2020-10, Vol.41 (20), p.1776-1783</ispartof><rights>2019 WILEY‐VCH Verlag GmbH & Co. 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(Lamiaceae) is an aromatic plant widely popular mainly due to its uses in traditional medicine as an anti‐inflammatory, diuretic and antimicrobial, as well as in the prevention and treatment of diseases. These biological activities are mainly related to the presence of phenolic and terpenic compounds. This work reports a chemical profile analysis of extracts from leaves and calli of rosemary obtained by both pressurized liquid extraction and maceration. Chemical profiles were determined on calli extracts of 3, 6, 9, and 15 days of culture; chemical characterization and quantification of compounds was carried out using ultrahigh performance liquid chromatography‐mass spectrometry. A total of 53 metabolites were identified in callus and 47 compounds in leaf extracts, of which 25 correspond to phenolic compounds, mainly flavonoids and flavones, 13 terpenes that include phenolic terpenes and one diterpenolactone, two glycosides which correspond to 6‐O‐caffeoyl‐β‐D‐fructofuranosil‐(2→1)‐α‐D‐glucopyranoside and primulaverin, an aromatic compound identified as fenantrenone and a growth regulator 12‐hydroxy jasmonic acid. These results showed that undifferentiated rosemary cells accumulate the same compounds identified mainly in highly specialized tissues such as leaves. The plant cell culture supply the possibility of developing biotechnological processes to obtain compounds of commercial interest.</description><subject>Antiinfectives and antibacterials</subject><subject>Aromatic compounds</subject><subject>Callus</subject><subject>Carnosic acid</subject><subject>Carnosol</subject><subject>Chromatography, High Pressure Liquid - methods</subject><subject>Cinnamates - analysis</subject><subject>Cinnamates - chemistry</subject><subject>Depsides - analysis</subject><subject>Depsides - chemistry</subject><subject>Diuretics</subject><subject>Flavonoids</subject><subject>Flavonoids - analysis</subject><subject>Flavonoids - chemistry</subject><subject>Glycosides - analysis</subject><subject>Glycosides - chemistry</subject><subject>Growth regulators</subject><subject>Jasmonic acid</subject><subject>Liquid chromatography</subject><subject>Mass spectrometry</subject><subject>Mass Spectrometry - methods</subject><subject>Metabolites</subject><subject>Phenols</subject><subject>Plant Extracts - chemistry</subject><subject>Plant Leaves - chemistry</subject><subject>Pressurized liquid extraction</subject><subject>Rosemary</subject><subject>Rosmarinic Acid</subject><subject>Rosmarinus - chemistry</subject><subject>Terpenes</subject><subject>Terpenes - analysis</subject><subject>Terpenes - chemistry</subject><issn>0173-0835</issn><issn>1522-2683</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2020</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqF0L1OwzAUBWALgWgprIwoEgtLyr1OHDsjqviTikBQViLXsYUrNylxA5SJR-AZeRIcFRhYWGxZ_nzkewjZRxgiAD3WbuGHFDAHQEY3SD-sNKaZSDZJH5AnMYiE9ciO9zMASPM03Sa9BGnGmUj75GH0qOdWSRepR9lItdSNfZNLW1dRbSKn5bP2kazKKBBnI_267JDvLm9rP5eNrdruZKyylXTWR9NVdH9xMx59vn9c3e2SLSOd13vf-4BMzk4no4t4fH1-OToZxyoFRuNsalKFJVVlzvgUtMDSCKa4zMNokqNkUGbaJBkvlVRSG84BBaJgUqDhyYAcrWMXTf3Uar8s5tYr7ZysdN36glKGmcgzzAM9_ENndduErweVMuAMMe_UcK1UU3vfaFMsGhumXRUIRVd80RVf_BYfHhx8x7bTuS5_-U_TAaRr8GKdXv0TV5yOb-44UJp8AZpXjyw</recordid><startdate>202010</startdate><enddate>202010</enddate><creator>Pérez‐Mendoza, Martha Berenice</creator><creator>Llorens‐Escobar, Laura</creator><creator>Vanegas‐Espinoza, Pablo Emilio</creator><creator>Cifuentes, Alejandro</creator><creator>Ibáñez, Elena</creator><creator>Villar‐Martínez, Alma Angélica Del</creator><general>Wiley Subscription Services, Inc</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7U5</scope><scope>8FD</scope><scope>L7M</scope><scope>7X8</scope><orcidid>https://orcid.org/0000-0002-9967-4501</orcidid><orcidid>https://orcid.org/0000-0002-7464-0217</orcidid></search><sort><creationdate>202010</creationdate><title>Chemical characterization of leaves and calli extracts of Rosmarinus officinalis by UHPLC‐MS</title><author>Pérez‐Mendoza, Martha Berenice ; Llorens‐Escobar, Laura ; Vanegas‐Espinoza, Pablo Emilio ; Cifuentes, Alejandro ; Ibáñez, Elena ; Villar‐Martínez, Alma Angélica Del</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c4052-6bf4c1d2cd957b0e81df85c7a9201a71a50d6ef367dcacaef770181185a81f73</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2020</creationdate><topic>Antiinfectives and antibacterials</topic><topic>Aromatic compounds</topic><topic>Callus</topic><topic>Carnosic acid</topic><topic>Carnosol</topic><topic>Chromatography, High Pressure Liquid - methods</topic><topic>Cinnamates - analysis</topic><topic>Cinnamates - chemistry</topic><topic>Depsides - analysis</topic><topic>Depsides - chemistry</topic><topic>Diuretics</topic><topic>Flavonoids</topic><topic>Flavonoids - analysis</topic><topic>Flavonoids - chemistry</topic><topic>Glycosides - analysis</topic><topic>Glycosides - chemistry</topic><topic>Growth regulators</topic><topic>Jasmonic acid</topic><topic>Liquid chromatography</topic><topic>Mass spectrometry</topic><topic>Mass Spectrometry - methods</topic><topic>Metabolites</topic><topic>Phenols</topic><topic>Plant Extracts - chemistry</topic><topic>Plant Leaves - chemistry</topic><topic>Pressurized liquid extraction</topic><topic>Rosemary</topic><topic>Rosmarinic Acid</topic><topic>Rosmarinus - chemistry</topic><topic>Terpenes</topic><topic>Terpenes - analysis</topic><topic>Terpenes - chemistry</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Pérez‐Mendoza, Martha Berenice</creatorcontrib><creatorcontrib>Llorens‐Escobar, Laura</creatorcontrib><creatorcontrib>Vanegas‐Espinoza, Pablo Emilio</creatorcontrib><creatorcontrib>Cifuentes, Alejandro</creatorcontrib><creatorcontrib>Ibáñez, Elena</creatorcontrib><creatorcontrib>Villar‐Martínez, Alma Angélica Del</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Solid State and Superconductivity Abstracts</collection><collection>Technology Research Database</collection><collection>Advanced Technologies Database with Aerospace</collection><collection>MEDLINE - Academic</collection><jtitle>Electrophoresis</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Pérez‐Mendoza, Martha Berenice</au><au>Llorens‐Escobar, Laura</au><au>Vanegas‐Espinoza, Pablo Emilio</au><au>Cifuentes, Alejandro</au><au>Ibáñez, Elena</au><au>Villar‐Martínez, Alma Angélica Del</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Chemical characterization of leaves and calli extracts of Rosmarinus officinalis by UHPLC‐MS</atitle><jtitle>Electrophoresis</jtitle><addtitle>Electrophoresis</addtitle><date>2020-10</date><risdate>2020</risdate><volume>41</volume><issue>20</issue><spage>1776</spage><epage>1783</epage><pages>1776-1783</pages><issn>0173-0835</issn><eissn>1522-2683</eissn><abstract>Rosmarinus officinalis L. (Lamiaceae) is an aromatic plant widely popular mainly due to its uses in traditional medicine as an anti‐inflammatory, diuretic and antimicrobial, as well as in the prevention and treatment of diseases. These biological activities are mainly related to the presence of phenolic and terpenic compounds. This work reports a chemical profile analysis of extracts from leaves and calli of rosemary obtained by both pressurized liquid extraction and maceration. Chemical profiles were determined on calli extracts of 3, 6, 9, and 15 days of culture; chemical characterization and quantification of compounds was carried out using ultrahigh performance liquid chromatography‐mass spectrometry. A total of 53 metabolites were identified in callus and 47 compounds in leaf extracts, of which 25 correspond to phenolic compounds, mainly flavonoids and flavones, 13 terpenes that include phenolic terpenes and one diterpenolactone, two glycosides which correspond to 6‐O‐caffeoyl‐β‐D‐fructofuranosil‐(2→1)‐α‐D‐glucopyranoside and primulaverin, an aromatic compound identified as fenantrenone and a growth regulator 12‐hydroxy jasmonic acid. These results showed that undifferentiated rosemary cells accumulate the same compounds identified mainly in highly specialized tissues such as leaves. The plant cell culture supply the possibility of developing biotechnological processes to obtain compounds of commercial interest.</abstract><cop>Germany</cop><pub>Wiley Subscription Services, Inc</pub><pmid>31267584</pmid><doi>10.1002/elps.201900152</doi><tpages>8</tpages><orcidid>https://orcid.org/0000-0002-9967-4501</orcidid><orcidid>https://orcid.org/0000-0002-7464-0217</orcidid></addata></record> |
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| subjects | Antiinfectives and antibacterials Aromatic compounds Callus Carnosic acid Carnosol Chromatography, High Pressure Liquid - methods Cinnamates - analysis Cinnamates - chemistry Depsides - analysis Depsides - chemistry Diuretics Flavonoids Flavonoids - analysis Flavonoids - chemistry Glycosides - analysis Glycosides - chemistry Growth regulators Jasmonic acid Liquid chromatography Mass spectrometry Mass Spectrometry - methods Metabolites Phenols Plant Extracts - chemistry Plant Leaves - chemistry Pressurized liquid extraction Rosemary Rosmarinic Acid Rosmarinus - chemistry Terpenes Terpenes - analysis Terpenes - chemistry |
| title | Chemical characterization of leaves and calli extracts of Rosmarinus officinalis by UHPLC‐MS |
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