N-glycosylation and homodimeric folding significantly enhance the immunoreactivity of Mycobacterium tuberculosis virulence factor CFP32 when produced in the yeast Pichia pastoris

We previously reported that immunoreactivity of recombinant CFP32 (Rv0577), a virulence factor of Mycobacterium tuberculosis, was higher when produced in transformed Pichia pastoris as compared to transformed E. coli. In this study, we show that this difference is partly due to the N-glycosylation o...

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Veröffentlicht in:	Biochemical and biophysical research communications 2019-08, Vol.516 (3), p.845-850
Hauptverfasser:	Benabdessalem, Chaouki, Othman, Houcemeddine, Ouni, Rym, Ghouibi, Nabila, Dahman, Amira, Riahi, Rachid, Larguach, Beya, Jihene bettaieb, Srairi-Abid, Najet, Barbouche, Mohamed-Ridha, Fathallah, M. Dahmani
Format:	Artikel
Sprache:	eng
Schlagworte:	CFP32 Immunogenicity N-glycosylation Pichia pastoris
Online-Zugang:	Volltext
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